Fraying and electron autodetachment dynamics of trapped gas phase oligonucleotides.
Identifieur interne : 002424 ( PubMed/Curation ); précédent : 002423; suivant : 002425Fraying and electron autodetachment dynamics of trapped gas phase oligonucleotides.
Auteurs : Allison S. Danell [États-Unis] ; Joel H. ParksSource :
- Journal of the American Society for Mass Spectrometry [ 1044-0305 ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Oligonucleotides.
- chemical : Boron Compounds, Fluorescent Dyes.
- Electrons, Models, Molecular, Nanotechnology, Spectrometry, Fluorescence, Spectrometry, Mass, Electrospray Ionization, Temperature.
Abstract
Sensitive methods recently developed to measure laser-induced fluorescence from trapped ions have been applied to study the dynamics of double- and single-stranded oligonucleotides. In this paper, the fraying of duplex terminal base pairs has been identified by measuring the donor fluorescence as a function of temperature from an oligonucleotide duplex labeled with a pair of FRET dyes. Comparison of the degree of dissociation of 14-mer duplexes observed in the mass spectra with the fluorescence intensity of the donor enables intermediate conformations of the unzipping duplex at the weaker binding end of the duplex to be identified. The autodetachment of electrons from double- and single-stranded oligonucleotide anions has been observed in a gas phase environment. To characterize this process, measurements were performed on 7-mers prepared without FRET fluorophores attached. The dependence of the decay rates of trapped anions have been measured as a function of charge state and temperature for various base compositions. An exceptionally strong dependence of the decay rate on base composition has been identified. The physical basis for this process will be discussed.
DOI: 10.1016/S1044-0305(03)00578-6
PubMed: 14652182
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<term>Spectrometry, Fluorescence</term>
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<term>Modèles moléculaires</term>
<term>Nanotechnologie</term>
<term>Oligonucléotides ()</term>
<term>Spectrométrie de fluorescence</term>
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<term>Oligonucléotides</term>
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<front><div type="abstract" xml:lang="en">Sensitive methods recently developed to measure laser-induced fluorescence from trapped ions have been applied to study the dynamics of double- and single-stranded oligonucleotides. In this paper, the fraying of duplex terminal base pairs has been identified by measuring the donor fluorescence as a function of temperature from an oligonucleotide duplex labeled with a pair of FRET dyes. Comparison of the degree of dissociation of 14-mer duplexes observed in the mass spectra with the fluorescence intensity of the donor enables intermediate conformations of the unzipping duplex at the weaker binding end of the duplex to be identified. The autodetachment of electrons from double- and single-stranded oligonucleotide anions has been observed in a gas phase environment. To characterize this process, measurements were performed on 7-mers prepared without FRET fluorophores attached. The dependence of the decay rates of trapped anions have been measured as a function of charge state and temperature for various base compositions. An exceptionally strong dependence of the decay rate on base composition has been identified. The physical basis for this process will be discussed.</div>
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<Abstract><AbstractText>Sensitive methods recently developed to measure laser-induced fluorescence from trapped ions have been applied to study the dynamics of double- and single-stranded oligonucleotides. In this paper, the fraying of duplex terminal base pairs has been identified by measuring the donor fluorescence as a function of temperature from an oligonucleotide duplex labeled with a pair of FRET dyes. Comparison of the degree of dissociation of 14-mer duplexes observed in the mass spectra with the fluorescence intensity of the donor enables intermediate conformations of the unzipping duplex at the weaker binding end of the duplex to be identified. The autodetachment of electrons from double- and single-stranded oligonucleotide anions has been observed in a gas phase environment. To characterize this process, measurements were performed on 7-mers prepared without FRET fluorophores attached. The dependence of the decay rates of trapped anions have been measured as a function of charge state and temperature for various base compositions. An exceptionally strong dependence of the decay rate on base composition has been identified. The physical basis for this process will be discussed.</AbstractText>
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<ReferenceList><Reference><Citation>J Mol Biol. 1999 Jan 8;285(1):245-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9878403</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chem Rev. 1999 Oct 13;99(10):3037-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11749510</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Struct Biol. 2000 Sep;7(9):730-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10966639</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 1995 Jan 10;34(1):293-300</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7819210</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phys Rev A Gen Phys. 1989 Oct 15;40(8):4392-4399</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9902681</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biophys J. 2003 May;84(5):3212-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12719250</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Am Chem Soc. 1998 Sep 23;120(37):9605-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16498487</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phys Rev Lett. 2000 Sep 11;85(11):2400-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10978020</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Phys Chem. 2000;51:179-207</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11031280</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4156-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11929991</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Am Soc Mass Spectrom. 2002 Jun;13(6):696-708</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12056569</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8602-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9671724</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
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