Transcription in vivo within the replication origin of the Escherichia coli chromosome: a mechanism for activating initiation of replication
Identifieur interne : 004794 ( Main/Exploration ); précédent : 004793; suivant : 004795Transcription in vivo within the replication origin of the Escherichia coli chromosome: a mechanism for activating initiation of replication
Auteurs : Tsuneaki Asai [Japon] ; Chi-Pien Chen [Taïwan] ; Toshio Nagata [Japon] ; Mitsuru Takanami [Japon] ; Mutsuo Imai [Japon]Source :
- Molecular and General Genetics MGG [ 0026-8925 ] ; 1992-01-01.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- Base Sequence, Chromosomes, Bacterial, DNA Replication, DnaA protein, Escherichia coli (genetics), Gene Expression Regulation, Bacterial, Molecular Sequence Data, Promoter Regions, Genetic, Replication initiation, Replicon, Transcription regulation, Transcription, Genetic, Transcriptional activation, oriC.
- MESH :
- genetics : Escherichia coli.
- Teeft :
- Amber mutation, Asai, Bacterial strains, Bamhi, Base Sequence, Biol, Chromosomal, Chromosome, Chromosomes, Bacterial, Coli, Coli chromosome, Copy number, DNA Replication, Deletion, Dnaa, Dnaa boxes, Dnaa gene, Dnaa protein, Dnaa strain, Duplex, Duplex opening, Escherichia, Escherichia coli, Escherichia coli chromosome, Fragment, Gene Expression Regulation, Bacterial, Genet, Hindiii, Initiation event, Kornberg, Leftward, Leftward origin transcripts, Leftward transcription, Minimal oric, Molecular Sequence Data, Mutation, Negative supercoiling, Nozaki, Nucleic acids, Oric, Oric fragment, Oric fragments, Oric plasmid replication, Oric plasmids, Oric region, Oric sequence, Origin activity, Phage, Plasmid, Polymerase, Positive supercoiling, Present work, Proc natl acad, Promoter, Promoter Regions, Genetic, Promoter fragment, Replication, Replication origin, Replicon, Right side, Rightward, Rightward origin transcription, Rightward origin transcripts, Rightward synthesis, Rightward transcription, Rightward transcripts, Rpob mutations, Sali, Schauzu, Sugimoto, Supercoiled, Supercoiling, Takanami, Transcript, Transcription, Transcription proceeds, Transcription, Genetic, Transcriptional, Transcriptional activation, Xhoi, Xhoi sites.
Abstract
Summary: Within the replication origin, oriC, of the Escherichia coli chromosome, novel in vivo transcripts were detected which proceeded rightward and whose production was activated by DnaA protein. In contrast, DnaA protein repressed the previously described ori-L leftward transcription. The former should introduce negative supercoiling, and the latter positive supercoiling, into the 13-mers. The effects of transcription on the initiation of replication were also investigated by making constructs with promoters placed near oriC. Transcription was found to enhance the origin activity only when it was oriented in such a way as to introduce negative supercoiling into the 13-mers. From these results, we propose that transcription within oriC regulates replication initiation by altering the topology of the 13-mer region.
Url:
DOI: 10.1007/BF00279788
Affiliations:
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Le document en format XML
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<term>DNA Replication</term>
<term>DnaA protein</term>
<term>Escherichia coli (genetics)</term>
<term>Gene Expression Regulation, Bacterial</term>
<term>Molecular Sequence Data</term>
<term>Promoter Regions, Genetic</term>
<term>Replication initiation</term>
<term>Replicon</term>
<term>Transcription regulation</term>
<term>Transcription, Genetic</term>
<term>Transcriptional activation</term>
<term>oriC</term>
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<term>Données de séquences moléculaires</term>
<term>Escherichia coli (génétique)</term>
<term>Régions promotrices (génétique)</term>
<term>Régulation de l'expression des gènes bactériens</term>
<term>Réplication de l'ADN</term>
<term>Réplicon</term>
<term>Séquence nucléotidique</term>
<term>Transcription génétique</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
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<term>Asai</term>
<term>Bacterial strains</term>
<term>Bamhi</term>
<term>Base Sequence</term>
<term>Biol</term>
<term>Chromosomal</term>
<term>Chromosome</term>
<term>Chromosomes, Bacterial</term>
<term>Coli</term>
<term>Coli chromosome</term>
<term>Copy number</term>
<term>DNA Replication</term>
<term>Deletion</term>
<term>Dnaa</term>
<term>Dnaa boxes</term>
<term>Dnaa gene</term>
<term>Dnaa protein</term>
<term>Dnaa strain</term>
<term>Duplex</term>
<term>Duplex opening</term>
<term>Escherichia</term>
<term>Escherichia coli</term>
<term>Escherichia coli chromosome</term>
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<term>Gene Expression Regulation, Bacterial</term>
<term>Genet</term>
<term>Hindiii</term>
<term>Initiation event</term>
<term>Kornberg</term>
<term>Leftward</term>
<term>Leftward origin transcripts</term>
<term>Leftward transcription</term>
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<term>Mutation</term>
<term>Negative supercoiling</term>
<term>Nozaki</term>
<term>Nucleic acids</term>
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<term>Oric fragment</term>
<term>Oric fragments</term>
<term>Oric plasmid replication</term>
<term>Oric plasmids</term>
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<term>Oric sequence</term>
<term>Origin activity</term>
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<term>Plasmid</term>
<term>Polymerase</term>
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<term>Present work</term>
<term>Proc natl acad</term>
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<term>Promoter Regions, Genetic</term>
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<term>Rightward origin transcription</term>
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<term>Rightward transcripts</term>
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<term>Schauzu</term>
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<term>Supercoiling</term>
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<term>Transcription</term>
<term>Transcription proceeds</term>
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<term>Transcriptional activation</term>
<term>Xhoi</term>
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<term>Régulation de l'expression des gènes bactériens</term>
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<term>Réplicon</term>
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<front><div type="abstract" xml:lang="en">Summary: Within the replication origin, oriC, of the Escherichia coli chromosome, novel in vivo transcripts were detected which proceeded rightward and whose production was activated by DnaA protein. In contrast, DnaA protein repressed the previously described ori-L leftward transcription. The former should introduce negative supercoiling, and the latter positive supercoiling, into the 13-mers. The effects of transcription on the initiation of replication were also investigated by making constructs with promoters placed near oriC. Transcription was found to enhance the origin activity only when it was oriented in such a way as to introduce negative supercoiling into the 13-mers. From these results, we propose that transcription within oriC regulates replication initiation by altering the topology of the 13-mer region.</div>
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