Self-stabilized antisense oligodeoxynucleotide phosphorothioates: properties and anti-HIV activity
Identifieur interne : 004544 ( Main/Exploration ); précédent : 004543; suivant : 004545Self-stabilized antisense oligodeoxynucleotide phosphorothioates: properties and anti-HIV activity
Auteurs : Jin Yan Tang [États-Unis] ; Jamal Temsamani [États-Unis] ; Sudhir Agrawal [États-Unis]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 1993.
Abstract
A new class of oligodeoxyribonucleotides has been designed, referred to here as ‘self-stabilized’ oligonucleotides. These oligonucleotides have hairpin loop structures at their 3′ ends, and show increased resistance to degradation by snake venom phosphodiesterase, DNA polymerase I and fetal bovine serum. The self-stabilized region of the oligonucleotide does not interfere in hybridization with complementary nucleic acids as shown by melting temperature, mobility-shift and RNase H cleavage studies. Various self-stabilized oligonucleotides containing increasingly stable hairpin loop regions were studied for their anti-HIV activity. Pharmacokinetic and stability studies in mice showed increased In vivo persistence of self-stabilized oligonucleotides with respect to their linear counterparts.
Url:
DOI: 10.1093/nar/21.11.2729
Affiliations:
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