Translational modulation Invitro of a eukaryotic viral mRNA encoding overlapping genes: Ribosome scanning and potential roles of conformational changes in the PC mRNA of sendai virus
Identifieur interne : 004E12 ( Main/Exploration ); précédent : 004E11; suivant : 004E13Translational modulation Invitro of a eukaryotic viral mRNA encoding overlapping genes: Ribosome scanning and potential roles of conformational changes in the PC mRNA of sendai virus
Auteurs : Kailash C. Gupta [États-Unis] ; David W. Kingsbury [États-Unis]Source :
- Biochemical and Biophysical Research Communications [ 0006-291X ] ; 1985.
English descriptors
- Teeft :
Abstract
Abstract: Expression of proteins from three overlapping genes in a single mRNA species of Sendai virus was modulated in a cell-free rabbit reticulocyte translation system. Hybrid-arrested translation by oligodeoxynucleotides complementary to specific regions of the mRNA that specifies the viral P, C, and C′ proteins demonstrated that ribosomes scan the RNA from its 5′ end to find initiation codons, and suggested that the secondary structure of the mRNA influences the selection of alternative initiation codons. Translational modulation of P, C, and C′ proteins by Mg++ and spermidine indicated that RNA folding is involved in this selection process.
Url:
DOI: 10.1016/0006-291X(85)91774-7
Affiliations:
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Gupta</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Virology and Molecular Biology St. Jude Children's Research Hospital 332 N. Lauderdale, P.O. Box 318 Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Kingsbury, David W" sort="Kingsbury, David W" uniqKey="Kingsbury D" first="David W." last="Kingsbury">David W. Kingsbury</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Virology and Molecular Biology St. Jude Children's Research Hospital 332 N. Lauderdale, P.O. Box 318 Memphis</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Biochemical and Biophysical Research Communications</title><title level="j" type="abbrev">YBBRC</title><idno type="ISSN">0006-291X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1985">1985</date><biblScope unit="volume">131</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="91">91</biblScope><biblScope unit="page" to="97">97</biblScope></imprint><idno type="ISSN">0006-291X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-291X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Codon</term><term>Hairpin</term><term>Hybridization</term><term>Initiation</term><term>Initiation codons</term><term>Kingsbury</term><term>Mrna</term><term>Nucleic acids</term><term>Oligonucleotide</term><term>Oligonucleotides</term><term>Proteins codons</term><term>Research communications</term><term>Ribosome</term><term>Sendai</term><term>Specific regions</term><term>Spermidine</term><term>Translational</term><term>Translational modulation</term><term>Virol</term><term>Virology</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Expression of proteins from three overlapping genes in a single mRNA species of Sendai virus was modulated in a cell-free rabbit reticulocyte translation system. Hybrid-arrested translation by oligodeoxynucleotides complementary to specific regions of the mRNA that specifies the viral P, C, and C′ proteins demonstrated that ribosomes scan the RNA from its 5′ end to find initiation codons, and suggested that the secondary structure of the mRNA influences the selection of alternative initiation codons. Translational modulation of P, C, and C′ proteins by Mg++ and spermidine indicated that RNA folding is involved in this selection process.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Tennessee</li></region></list><tree><country name="États-Unis"><region name="Tennessee"><name sortKey="Gupta, Kailash C" sort="Gupta, Kailash C" uniqKey="Gupta K" first="Kailash C." last="Gupta">Kailash C. Gupta</name></region><name sortKey="Kingsbury, David W" sort="Kingsbury, David W" uniqKey="Kingsbury D" first="David W." last="Kingsbury">David W. 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