Translational modulation Invitro of a eukaryotic viral mRNA encoding overlapping genes: Ribosome scanning and potential roles of conformational changes in the PC mRNA of sendai virus
Identifieur interne : 000C91 ( Istex/Curation ); précédent : 000C90; suivant : 000C92Translational modulation Invitro of a eukaryotic viral mRNA encoding overlapping genes: Ribosome scanning and potential roles of conformational changes in the PC mRNA of sendai virus
Auteurs : Kailash C. Gupta [États-Unis] ; David W. Kingsbury [États-Unis]Source :
- Biochemical and Biophysical Research Communications [ 0006-291X ] ; 1985.
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Abstract
Abstract: Expression of proteins from three overlapping genes in a single mRNA species of Sendai virus was modulated in a cell-free rabbit reticulocyte translation system. Hybrid-arrested translation by oligodeoxynucleotides complementary to specific regions of the mRNA that specifies the viral P, C, and C′ proteins demonstrated that ribosomes scan the RNA from its 5′ end to find initiation codons, and suggested that the secondary structure of the mRNA influences the selection of alternative initiation codons. Translational modulation of P, C, and C′ proteins by Mg++ and spermidine indicated that RNA folding is involved in this selection process.
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DOI: 10.1016/0006-291X(85)91774-7
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ISTEX:10A9BE9C631C2FC4FA83C05A20CF34474ECB2135Le document en format XML
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<term>Codon</term>
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<term>Oligonucleotide</term>
<term>Oligonucleotides</term>
<term>Proteins codons</term>
<term>Research communications</term>
<term>Ribosome</term>
<term>Sendai</term>
<term>Specific regions</term>
<term>Spermidine</term>
<term>Translational</term>
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<term>Virol</term>
<term>Virology</term>
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<front><div type="abstract" xml:lang="en">Abstract: Expression of proteins from three overlapping genes in a single mRNA species of Sendai virus was modulated in a cell-free rabbit reticulocyte translation system. Hybrid-arrested translation by oligodeoxynucleotides complementary to specific regions of the mRNA that specifies the viral P, C, and C′ proteins demonstrated that ribosomes scan the RNA from its 5′ end to find initiation codons, and suggested that the secondary structure of the mRNA influences the selection of alternative initiation codons. Translational modulation of P, C, and C′ proteins by Mg++ and spermidine indicated that RNA folding is involved in this selection process.</div>
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