Functional mapping of the genome of the B19 (human) parvovirus by in vitro translation after negative hybrid selection.
Identifieur interne : 004B86 ( Main/Exploration ); précédent : 004B85; suivant : 004B87Functional mapping of the genome of the B19 (human) parvovirus by in vitro translation after negative hybrid selection.
Auteurs : K. Ozawa [États-Unis] ; J. Ayub ; N. YoungSource :
- Journal of virology [ 0022-538X ] ; 1988.
Descripteurs français
- KwdFr :
- MESH :
- génétique : ARN viral, Parvoviridae, Protéines virales.
- métabolisme : ARN viral, Oligodésoxyribonucléotides, Érythroblastes.
- Biosynthèse des protéines, Gènes viraux, Humains, Hybridation d'acides nucléiques.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : RNA, Viral, Viral Proteins.
- chemical , metabolism : Oligodeoxyribonucleotides, RNA, Viral.
- genetics : Parvoviridae.
- metabolism : Erythroblasts.
- Genes, Viral, Humans, Nucleic Acid Hybridization, Protein Biosynthesis.
Abstract
We have analyzed the coding capacity of B19 parvovirus transcripts by in vitro translation using the negative hybrid selection technique. Five different antisense oligonucleotides (18-mers) corresponding to different portions of the B19 genome were hybridized to RNA samples extracted from human erythroid bone marrow cells infected with B19 parvovirus in vitro, and RNase H was added to cleave specific B19 RNA molecules at selected sites. B19-specific translation products of these RNA samples were determined by immunoprecipitation. We localized the B19 nonstructural protein to the left-side transcript and the two capsid proteins to overlapping transcripts from the right side of the genome.
PubMed: 3373576
Affiliations:
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Le document en format XML
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Young</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1988">1988</date><idno type="RBID">pubmed:3373576</idno><idno type="pmid">3373576</idno><idno type="wicri:Area/PubMed/Corpus">002A58</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A58</idno><idno type="wicri:Area/PubMed/Curation">002A58</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A58</idno><idno type="wicri:Area/PubMed/Checkpoint">002901</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002901</idno><idno type="wicri:Area/Ncbi/Merge">002837</idno><idno type="wicri:Area/Ncbi/Curation">002837</idno><idno type="wicri:Area/Ncbi/Checkpoint">002837</idno><idno type="wicri:doubleKey">0022-538X:1988:Ozawa K:functional:mapping:of</idno><idno type="wicri:Area/Main/Merge">004C65</idno><idno type="wicri:Area/Main/Curation">004B86</idno><idno type="wicri:Area/Main/Exploration">004B86</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Functional mapping of the genome of the B19 (human) parvovirus by in vitro translation after negative hybrid selection.</title><author><name sortKey="Ozawa, K" sort="Ozawa, K" uniqKey="Ozawa K" first="K" last="Ozawa">K. Ozawa</name><affiliation wicri:level="2"><nlm:affiliation>Cell Biology Section, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Cell Biology Section, National Heart, Lung, and Blood Institute, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Ayub, J" sort="Ayub, J" uniqKey="Ayub J" first="J" last="Ayub">J. Ayub</name></author><author><name sortKey="Young, N" sort="Young, N" uniqKey="Young N" first="N" last="Young">N. Young</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="1988" type="published">1988</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Erythroblasts (metabolism)</term><term>Genes, Viral</term><term>Humans</term><term>Nucleic Acid Hybridization</term><term>Oligodeoxyribonucleotides (metabolism)</term><term>Parvoviridae (genetics)</term><term>Protein Biosynthesis</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (metabolism)</term><term>Viral Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>ARN viral (métabolisme)</term><term>Biosynthèse des protéines</term><term>Gènes viraux</term><term>Humains</term><term>Hybridation d'acides nucléiques</term><term>Oligodésoxyribonucléotides (métabolisme)</term><term>Parvoviridae (génétique)</term><term>Protéines virales (génétique)</term><term>Érythroblastes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Oligodeoxyribonucleotides</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Parvoviridae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Parvoviridae</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Erythroblasts</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term><term>Oligodésoxyribonucléotides</term><term>Érythroblastes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genes, Viral</term><term>Humans</term><term>Nucleic Acid Hybridization</term><term>Protein Biosynthesis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biosynthèse des protéines</term><term>Gènes viraux</term><term>Humains</term><term>Hybridation d'acides nucléiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have analyzed the coding capacity of B19 parvovirus transcripts by in vitro translation using the negative hybrid selection technique. Five different antisense oligonucleotides (18-mers) corresponding to different portions of the B19 genome were hybridized to RNA samples extracted from human erythroid bone marrow cells infected with B19 parvovirus in vitro, and RNase H was added to cleave specific B19 RNA molecules at selected sites. B19-specific translation products of these RNA samples were determined by immunoprecipitation. We localized the B19 nonstructural protein to the left-side transcript and the two capsid proteins to overlapping transcripts from the right side of the genome.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><noCountry><name sortKey="Ayub, J" sort="Ayub, J" uniqKey="Ayub J" first="J" last="Ayub">J. Ayub</name><name sortKey="Young, N" sort="Young, N" uniqKey="Young N" first="N" last="Young">N. Young</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Ozawa, K" sort="Ozawa, K" uniqKey="Ozawa K" first="K" last="Ozawa">K. Ozawa</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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