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Functional mapping of the genome of the B19 (human) parvovirus by in vitro translation after negative hybrid selection.

Identifieur interne : 002A58 ( PubMed/Curation ); précédent : 002A57; suivant : 002A59

Functional mapping of the genome of the B19 (human) parvovirus by in vitro translation after negative hybrid selection.

Auteurs : K. Ozawa [États-Unis] ; J. Ayub ; N. Young

Source :

Journal of virology [ 0022-538X ] ; 1988.

RBID : pubmed:3373576

Descripteurs français

KwdFr :
- ARN viral (génétique), ARN viral (métabolisme), Biosynthèse des protéines, Gènes viraux, Humains, Hybridation d'acides nucléiques, Oligodésoxyribonucléotides (métabolisme), Parvoviridae (génétique), Protéines virales (génétique), Érythroblastes (métabolisme).
MESH :
- génétique : ARN viral, Parvoviridae, Protéines virales.
- métabolisme : ARN viral, Oligodésoxyribonucléotides, Érythroblastes.
- Biosynthèse des protéines, Gènes viraux, Humains, Hybridation d'acides nucléiques.

English descriptors

KwdEn :
- Erythroblasts (metabolism), Genes, Viral, Humans, Nucleic Acid Hybridization, Oligodeoxyribonucleotides (metabolism), Parvoviridae (genetics), Protein Biosynthesis, RNA, Viral (genetics), RNA, Viral (metabolism), Viral Proteins (genetics).
MESH :
- chemical , genetics : RNA, Viral, Viral Proteins.
- chemical , metabolism : Oligodeoxyribonucleotides, RNA, Viral.
- genetics : Parvoviridae.
- metabolism : Erythroblasts.
- Genes, Viral, Humans, Nucleic Acid Hybridization, Protein Biosynthesis.

Abstract

We have analyzed the coding capacity of B19 parvovirus transcripts by in vitro translation using the negative hybrid selection technique. Five different antisense oligonucleotides (18-mers) corresponding to different portions of the B19 genome were hybridized to RNA samples extracted from human erythroid bone marrow cells infected with B19 parvovirus in vitro, and RNase H was added to cleave specific B19 RNA molecules at selected sites. B19-specific translation products of these RNA samples were determined by immunoprecipitation. We localized the B19 nonstructural protein to the left-side transcript and the two capsid proteins to overlapping transcripts from the right side of the genome.

PubMed: 3373576

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Le document en format XML

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<author><name sortKey="Ozawa, K" sort="Ozawa, K" uniqKey="Ozawa K" first="K" last="Ozawa">K. Ozawa</name>
<affiliation wicri:level="2"><nlm:affiliation>Cell Biology Section, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Maryland</region>
</placeName>
<wicri:cityArea>Cell Biology Section, National Heart, Lung, and Blood Institute, Bethesda</wicri:cityArea>
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<author><name sortKey="Ayub, J" sort="Ayub, J" uniqKey="Ayub J" first="J" last="Ayub">J. Ayub</name>
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<author><name sortKey="Young, N" sort="Young, N" uniqKey="Young N" first="N" last="Young">N. Young</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Erythroblasts (metabolism)</term>
<term>Genes, Viral</term>
<term>Humans</term>
<term>Nucleic Acid Hybridization</term>
<term>Oligodeoxyribonucleotides (metabolism)</term>
<term>Parvoviridae (genetics)</term>
<term>Protein Biosynthesis</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term>
<term>ARN viral (métabolisme)</term>
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<term>Gènes viraux</term>
<term>Humains</term>
<term>Hybridation d'acides nucléiques</term>
<term>Oligodésoxyribonucléotides (métabolisme)</term>
<term>Parvoviridae (génétique)</term>
<term>Protéines virales (génétique)</term>
<term>Érythroblastes (métabolisme)</term>
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<term>Parvoviridae</term>
<term>Protéines virales</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Erythroblasts</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term>
<term>Oligodésoxyribonucléotides</term>
<term>Érythroblastes</term>
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<term>Humans</term>
<term>Nucleic Acid Hybridization</term>
<term>Protein Biosynthesis</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Biosynthèse des protéines</term>
<term>Gènes viraux</term>
<term>Humains</term>
<term>Hybridation d'acides nucléiques</term>
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<front><div type="abstract" xml:lang="en">We have analyzed the coding capacity of B19 parvovirus transcripts by in vitro translation using the negative hybrid selection technique. Five different antisense oligonucleotides (18-mers) corresponding to different portions of the B19 genome were hybridized to RNA samples extracted from human erythroid bone marrow cells infected with B19 parvovirus in vitro, and RNase H was added to cleave specific B19 RNA molecules at selected sites. B19-specific translation products of these RNA samples were determined by immunoprecipitation. We localized the B19 nonstructural protein to the left-side transcript and the two capsid proteins to overlapping transcripts from the right side of the genome.</div>
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<Title>Journal of virology</Title>
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<Abstract><AbstractText>We have analyzed the coding capacity of B19 parvovirus transcripts by in vitro translation using the negative hybrid selection technique. Five different antisense oligonucleotides (18-mers) corresponding to different portions of the B19 genome were hybridized to RNA samples extracted from human erythroid bone marrow cells infected with B19 parvovirus in vitro, and RNase H was added to cleave specific B19 RNA molecules at selected sites. B19-specific translation products of these RNA samples were determined by immunoprecipitation. We localized the B19 nonstructural protein to the left-side transcript and the two capsid proteins to overlapping transcripts from the right side of the genome.</AbstractText>
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Functional mapping of the genome of the B19 (human) parvovirus by in vitro translation after negative hybrid selection.

Functional mapping of the genome of the B19 (human) parvovirus by in vitro translation after negative hybrid selection.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

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Pour générer des pages wiki