Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide
Identifieur interne : 004B29 ( Main/Exploration ); précédent : 004B28; suivant : 004B30Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide
Auteurs : John C. Pugh [États-Unis] ; Jesse W. Summers [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1989.
English descriptors
- Teeft :
- Culture medium, Cultured hepatocytes, Dhbcag, Dhbv, Dhbv infection, Dmso, Dmso medium, Duck hepatitis, Duck hepatocytes, Duck serum, Equivalent amounts, Hepatocyte, Hepatocyte cultures, Hepatocyte membrane, Hepatocytes, Hybridization, Indirect immunofluorescence, Infection, Infection cells, Inoculum, Maximal infection, Plating, Primary duck hepatocytes, Receptor, Replicative dhbv, Same amount, Southern blot hybridization, Surface antigen particles, Total intracellular, Tuttleman, Virus, Virus infection, Virus particles, Warm medium.
Abstract
Abstract: Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37° appears slow, with uptake occurring over a period of at least 16 hr.
Url:
DOI: 10.1016/0042-6822(89)90199-2
Affiliations:
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Le document en format XML
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Summers</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D746914D95FF6CB4EF85F36F0EFECC6B535561BF</idno><date when="1989" year="1989">1989</date><idno type="doi">10.1016/0042-6822(89)90199-2</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-47ND7VWV-7/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001714</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001714</idno><idno type="wicri:Area/Istex/Curation">001714</idno><idno type="wicri:Area/Istex/Checkpoint">002034</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002034</idno><idno type="wicri:doubleKey">0042-6822:1989:Pugh J:infection:and:uptake</idno><idno type="wicri:Area/Main/Merge">004C07</idno><idno type="wicri:Area/Main/Curation">004B29</idno><idno type="wicri:Area/Main/Exploration">004B29</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</title><author><name sortKey="Pugh, John C" sort="Pugh, John C" uniqKey="Pugh J" first="John C." last="Pugh">John C. Pugh</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111</wicri:regionArea><wicri:noRegion>Pennsylvania 19111</wicri:noRegion></affiliation></author><author><name sortKey="Summers, Jesse W" sort="Summers, Jesse W" uniqKey="Summers J" first="Jesse W." last="Summers">Jesse W. Summers</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111</wicri:regionArea><wicri:noRegion>Pennsylvania 19111</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989">1989</date><biblScope unit="volume">172</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="564">564</biblScope><biblScope unit="page" to="572">572</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Culture medium</term><term>Cultured hepatocytes</term><term>Dhbcag</term><term>Dhbv</term><term>Dhbv infection</term><term>Dmso</term><term>Dmso medium</term><term>Duck hepatitis</term><term>Duck hepatocytes</term><term>Duck serum</term><term>Equivalent amounts</term><term>Hepatocyte</term><term>Hepatocyte cultures</term><term>Hepatocyte membrane</term><term>Hepatocytes</term><term>Hybridization</term><term>Indirect immunofluorescence</term><term>Infection</term><term>Infection cells</term><term>Inoculum</term><term>Maximal infection</term><term>Plating</term><term>Primary duck hepatocytes</term><term>Receptor</term><term>Replicative dhbv</term><term>Same amount</term><term>Southern blot hybridization</term><term>Surface antigen particles</term><term>Total intracellular</term><term>Tuttleman</term><term>Virus</term><term>Virus infection</term><term>Virus particles</term><term>Warm medium</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37° appears slow, with uptake occurring over a period of at least 16 hr.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Pugh, John C" sort="Pugh, John C" uniqKey="Pugh J" first="John C." last="Pugh">John C. Pugh</name></noRegion><name sortKey="Summers, Jesse W" sort="Summers, Jesse W" uniqKey="Summers J" first="Jesse W." last="Summers">Jesse W. 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