Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide
Identifieur interne : 001714 ( Istex/Corpus ); précédent : 001713; suivant : 001715Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide
Auteurs : John C. Pugh ; Jesse W. SummersSource :
- Virology [ 0042-6822 ] ; 1989.
English descriptors
- Teeft :
- Culture medium, Cultured hepatocytes, Dhbcag, Dhbv, Dhbv infection, Dmso, Dmso medium, Duck hepatitis, Duck hepatocytes, Duck serum, Equivalent amounts, Hepatocyte, Hepatocyte cultures, Hepatocyte membrane, Hepatocytes, Hybridization, Indirect immunofluorescence, Infection, Infection cells, Inoculum, Maximal infection, Plating, Primary duck hepatocytes, Receptor, Replicative dhbv, Same amount, Southern blot hybridization, Surface antigen particles, Total intracellular, Tuttleman, Virus, Virus infection, Virus particles, Warm medium.
Abstract
Abstract: Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37° appears slow, with uptake occurring over a period of at least 16 hr.
Url:
DOI: 10.1016/0042-6822(89)90199-2
Links to Exploration step
ISTEX:D746914D95FF6CB4EF85F36F0EFECC6B535561BFLe document en format XML
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Pugh</name><affiliation><mods:affiliation>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</mods:affiliation></affiliation></author><author><name sortKey="Summers, Jesse W" sort="Summers, Jesse W" uniqKey="Summers J" first="Jesse W." last="Summers">Jesse W. Summers</name><affiliation><mods:affiliation>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989">1989</date><biblScope unit="volume">172</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="564">564</biblScope><biblScope unit="page" to="572">572</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Culture medium</term><term>Cultured hepatocytes</term><term>Dhbcag</term><term>Dhbv</term><term>Dhbv infection</term><term>Dmso</term><term>Dmso medium</term><term>Duck hepatitis</term><term>Duck hepatocytes</term><term>Duck serum</term><term>Equivalent amounts</term><term>Hepatocyte</term><term>Hepatocyte cultures</term><term>Hepatocyte membrane</term><term>Hepatocytes</term><term>Hybridization</term><term>Indirect immunofluorescence</term><term>Infection</term><term>Infection cells</term><term>Inoculum</term><term>Maximal infection</term><term>Plating</term><term>Primary duck hepatocytes</term><term>Receptor</term><term>Replicative dhbv</term><term>Same amount</term><term>Southern blot hybridization</term><term>Surface antigen particles</term><term>Total intracellular</term><term>Tuttleman</term><term>Virus</term><term>Virus infection</term><term>Virus particles</term><term>Warm medium</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. 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Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37° appears slow, with uptake occurring over a period of at least 16 hr.</abstract><qualityIndicators><score>8.38</score><pdfWordCount>5083</pdfWordCount><pdfCharCount>26338</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>115</abstractWordCount><abstractCharCount>734</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</title><pmid><json:string>2678730</json:string></pmid><pii><json:string>0042-6822(89)90199-2</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. Sci. 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Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37° appears slow, with uptake occurring over a period of at least 16 hr.</p></abstract></profileDesc><revisionDesc><change when="1989">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-47ND7VWV-7/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>89901992</aid><ce:pii>0042-6822(89)90199-2</ce:pii><ce:doi>10.1016/0042-6822(89)90199-2</ce:doi><ce:copyright type="unknown" year="1989"></ce:copyright></item-info><head><ce:title>Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</ce:title><ce:author-group><ce:author><ce:given-name>John C.</ce:given-name><ce:surname>Pugh</ce:surname><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jesse W.</ce:given-name><ce:surname>Summers</ce:surname><ce:cross-ref refid="FN1"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>To whom requests for reprints should be addressed at present address: Dept. of Medicine, St. Mary's Hospital Medical School, South Wharf Road, London W2 1 NY, UK.</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>2</ce:label><ce:note-para>Present address: School of Medicine, Department of Cell Biology, University of New Mexico, 900 Camino de Salud, NE, Albuquerque, NM 87131.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="2" month="11" year="1988"></ce:date-received><ce:date-accepted day="5" month="6" year="1989"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection <ce:italic>in vitro</ce:italic>. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37° appears slow, with uptake occurring over a period of at least 16 hr.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</title></titleInfo><name type="personal"><namePart type="given">John C.</namePart><namePart type="family">Pugh</namePart><affiliation>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</affiliation><description>To whom requests for reprints should be addressed at present address: Dept. of Medicine, St. Mary's Hospital Medical School, South Wharf Road, London W2 1 NY, UK.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jesse W.</namePart><namePart type="family">Summers</namePart><affiliation>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</affiliation><description>Present address: School of Medicine, Department of Cell Biology, University of New Mexico, 900 Camino de Salud, NE, Albuquerque, NM 87131.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1989</dateIssued><copyrightDate encoding="w3cdtf">1989</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. 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