Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide
Identifieur interne : 001714 ( Istex/Corpus ); précédent : 001713; suivant : 001715Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide
Auteurs : John C. Pugh ; Jesse W. SummersSource :
- Virology [ 0042-6822 ] ; 1989.
English descriptors
- Teeft :
- Culture medium, Cultured hepatocytes, Dhbcag, Dhbv, Dhbv infection, Dmso, Dmso medium, Duck hepatitis, Duck hepatocytes, Duck serum, Equivalent amounts, Hepatocyte, Hepatocyte cultures, Hepatocyte membrane, Hepatocytes, Hybridization, Indirect immunofluorescence, Infection, Infection cells, Inoculum, Maximal infection, Plating, Primary duck hepatocytes, Receptor, Replicative dhbv, Same amount, Southern blot hybridization, Surface antigen particles, Total intracellular, Tuttleman, Virus, Virus infection, Virus particles, Warm medium.
Abstract
Abstract: Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37° appears slow, with uptake occurring over a period of at least 16 hr.
Url:
DOI: 10.1016/0042-6822(89)90199-2
Links to Exploration step
ISTEX:D746914D95FF6CB4EF85F36F0EFECC6B535561BFLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</title>
<author><name sortKey="Pugh, John C" sort="Pugh, John C" uniqKey="Pugh J" first="John C." last="Pugh">John C. Pugh</name>
<affiliation><mods:affiliation>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Summers, Jesse W" sort="Summers, Jesse W" uniqKey="Summers J" first="Jesse W." last="Summers">Jesse W. Summers</name>
<affiliation><mods:affiliation>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</mods:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:D746914D95FF6CB4EF85F36F0EFECC6B535561BF</idno>
<date when="1989" year="1989">1989</date>
<idno type="doi">10.1016/0042-6822(89)90199-2</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-47ND7VWV-7/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001714</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001714</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</title>
<author><name sortKey="Pugh, John C" sort="Pugh, John C" uniqKey="Pugh J" first="John C." last="Pugh">John C. Pugh</name>
<affiliation><mods:affiliation>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Summers, Jesse W" sort="Summers, Jesse W" uniqKey="Summers J" first="Jesse W." last="Summers">Jesse W. Summers</name>
<affiliation><mods:affiliation>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</mods:affiliation>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Virology</title>
<title level="j" type="abbrev">YVIRO</title>
<idno type="ISSN">0042-6822</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1989">1989</date>
<biblScope unit="volume">172</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="564">564</biblScope>
<biblScope unit="page" to="572">572</biblScope>
</imprint>
<idno type="ISSN">0042-6822</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0042-6822</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Culture medium</term>
<term>Cultured hepatocytes</term>
<term>Dhbcag</term>
<term>Dhbv</term>
<term>Dhbv infection</term>
<term>Dmso</term>
<term>Dmso medium</term>
<term>Duck hepatitis</term>
<term>Duck hepatocytes</term>
<term>Duck serum</term>
<term>Equivalent amounts</term>
<term>Hepatocyte</term>
<term>Hepatocyte cultures</term>
<term>Hepatocyte membrane</term>
<term>Hepatocytes</term>
<term>Hybridization</term>
<term>Indirect immunofluorescence</term>
<term>Infection</term>
<term>Infection cells</term>
<term>Inoculum</term>
<term>Maximal infection</term>
<term>Plating</term>
<term>Primary duck hepatocytes</term>
<term>Receptor</term>
<term>Replicative dhbv</term>
<term>Same amount</term>
<term>Southern blot hybridization</term>
<term>Surface antigen particles</term>
<term>Total intracellular</term>
<term>Tuttleman</term>
<term>Virus</term>
<term>Virus infection</term>
<term>Virus particles</term>
<term>Warm medium</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37° appears slow, with uptake occurring over a period of at least 16 hr.</div>
</front>
</TEI>
<istex><corpusName>elsevier</corpusName>
<keywords><teeft><json:string>dhbv</json:string>
<json:string>hepatocytes</json:string>
<json:string>dmso</json:string>
<json:string>receptor</json:string>
<json:string>dmso medium</json:string>
<json:string>hepatocyte</json:string>
<json:string>dhbcag</json:string>
<json:string>inoculum</json:string>
<json:string>plating</json:string>
<json:string>hybridization</json:string>
<json:string>duck hepatitis</json:string>
<json:string>southern blot hybridization</json:string>
<json:string>tuttleman</json:string>
<json:string>virus infection</json:string>
<json:string>virus particles</json:string>
<json:string>duck hepatocytes</json:string>
<json:string>dhbv infection</json:string>
<json:string>total intracellular</json:string>
<json:string>infection</json:string>
<json:string>hepatocyte cultures</json:string>
<json:string>cultured hepatocytes</json:string>
<json:string>infection cells</json:string>
<json:string>primary duck hepatocytes</json:string>
<json:string>culture medium</json:string>
<json:string>replicative dhbv</json:string>
<json:string>virus</json:string>
<json:string>surface antigen particles</json:string>
<json:string>hepatocyte membrane</json:string>
<json:string>indirect immunofluorescence</json:string>
<json:string>maximal infection</json:string>
<json:string>same amount</json:string>
<json:string>duck serum</json:string>
<json:string>warm medium</json:string>
<json:string>equivalent amounts</json:string>
</teeft>
</keywords>
<author><json:item><name>John C. Pugh</name>
<affiliations><json:string>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</json:string>
</affiliations>
</json:item>
<json:item><name>Jesse W. Summers</name>
<affiliations><json:string>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</json:string>
</affiliations>
</json:item>
</author>
<arkIstex>ark:/67375/6H6-47ND7VWV-7</arkIstex>
<language><json:string>eng</json:string>
</language>
<originalGenre><json:string>Full-length article</json:string>
</originalGenre>
<abstract>Abstract: Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37° appears slow, with uptake occurring over a period of at least 16 hr.</abstract>
<qualityIndicators><score>8.38</score>
<pdfWordCount>5083</pdfWordCount>
<pdfCharCount>26338</pdfCharCount>
<pdfVersion>1.4</pdfVersion>
<pdfPageCount>9</pdfPageCount>
<pdfPageSize>612 x 792 pts (letter)</pdfPageSize>
<refBibsNative>true</refBibsNative>
<abstractWordCount>115</abstractWordCount>
<abstractCharCount>734</abstractCharCount>
<keywordCount>0</keywordCount>
</qualityIndicators>
<title>Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</title>
<pmid><json:string>2678730</json:string>
</pmid>
<pii><json:string>0042-6822(89)90199-2</json:string>
</pii>
<genre><json:string>research-article</json:string>
</genre>
<serie><title>Proc. Natl. Acad. Sci. USA</title>
<language><json:string>unknown</json:string>
</language>
<volume>81</volume>
<pages><first>1411</first>
<last>1415</last>
</pages>
</serie>
<host><title>Virology</title>
<language><json:string>unknown</json:string>
</language>
<publicationDate>1989</publicationDate>
<issn><json:string>0042-6822</json:string>
</issn>
<pii><json:string>S0042-6822(00)X0263-2</json:string>
</pii>
<volume>172</volume>
<issue>2</issue>
<pages><first>564</first>
<last>572</last>
</pages>
<genre><json:string>journal</json:string>
</genre>
</host>
<namedEntities><unitex><date><json:string>1989</json:string>
</date>
<geogName></geogName>
<orgName><json:string>Department of Cell Biology, University of New Mexico</json:string>
<json:string>National Institutes of Health</json:string>
<json:string>Mary's Hospital Medical School, South Wharf Road, London W</json:string>
<json:string>Public Health Service Grant CA-42542</json:string>
<json:string>RR</json:string>
<json:string>Public Health Service Grants CA06927</json:string>
</orgName>
<orgName_funder><json:string>Public Health Service Grant CA-42542</json:string>
<json:string>RR</json:string>
<json:string>Public Health Service Grants CA06927</json:string>
</orgName_funder>
<orgName_provider></orgName_provider>
<persName><json:string>Carol Aldrich</json:string>
<json:string>William Mason</json:string>
<json:string>Carrie House</json:string>
<json:string>Bill Mason</json:string>
<json:string>Laura Coates</json:string>
</persName>
<placeName><json:string>Albuquerque</json:string>
<json:string>NM</json:string>
</placeName>
<ref_url></ref_url>
<ref_bibl><json:string>Steck and Rubin, 1966a,b</json:string>
<json:string>Mason et al</json:string>
<json:string>Tuttleman et al.</json:string>
<json:string>Guguen-Guillouzo et al., 1983</json:string>
<json:string>Institute for Cancer Research, Fox Chase Cancer Center, 7701</json:string>
<json:string>Tuttleman et al</json:string>
<json:string>Tuttleman etat, 1986b</json:string>
<json:string>Berry and Friend, 1969</json:string>
<json:string>Summers and Mason, 1982</json:string>
<json:string>Isom et al., 1985, 1987</json:string>
<json:string>Pugh et al., 1988</json:string>
</ref_bibl>
<bibl></bibl>
</unitex>
</namedEntities>
<ark><json:string>ark:/67375/6H6-47ND7VWV-7</json:string>
</ark>
<categories><wos><json:string>1 - science</json:string>
<json:string>2 - virology</json:string>
</wos>
<scienceMetrix><json:string>1 - health sciences</json:string>
<json:string>2 - biomedical research</json:string>
<json:string>3 - virology</json:string>
</scienceMetrix>
<scopus><json:string>1 - Life Sciences</json:string>
<json:string>2 - Immunology and Microbiology</json:string>
<json:string>3 - Virology</json:string>
</scopus>
<inist><json:string>1 - sciences appliquees, technologies et medecines</json:string>
<json:string>2 - sciences biologiques et medicales</json:string>
<json:string>3 - sciences medicales</json:string>
<json:string>4 - hemopathies</json:string>
</inist>
</categories>
<publicationDate>1989</publicationDate>
<copyrightDate>1989</copyrightDate>
<doi><json:string>10.1016/0042-6822(89)90199-2</json:string>
</doi>
<id>D746914D95FF6CB4EF85F36F0EFECC6B535561BF</id>
<score>1</score>
<fulltext><json:item><extension>pdf</extension>
<original>true</original>
<mimetype>application/pdf</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-47ND7VWV-7/fulltext.pdf</uri>
</json:item>
<json:item><extension>zip</extension>
<original>false</original>
<mimetype>application/zip</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-47ND7VWV-7/bundle.zip</uri>
</json:item>
<istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-47ND7VWV-7/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</title>
</titleStmt>
<publicationStmt><authority>ISTEX</authority>
<publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher>
<availability><licence><p>elsevier</p>
</licence>
</availability>
<p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p>
<date>1989</date>
</publicationStmt>
<notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note>
<note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note>
</notesStmt>
<sourceDesc><biblStruct type="inbook"><analytic><title level="a">Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</title>
<author xml:id="author-0000"><persName><forename type="first">John C.</forename>
<surname>Pugh</surname>
</persName>
<note type="biography">To whom requests for reprints should be addressed at present address: Dept. of Medicine, St. Mary's Hospital Medical School, South Wharf Road, London W2 1 NY, UK.</note>
<affiliation>To whom requests for reprints should be addressed at present address: Dept. of Medicine, St. Mary's Hospital Medical School, South Wharf Road, London W2 1 NY, UK.</affiliation>
<affiliation>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</affiliation>
</author>
<author xml:id="author-0001"><persName><forename type="first">Jesse W.</forename>
<surname>Summers</surname>
</persName>
<note type="biography">Present address: School of Medicine, Department of Cell Biology, University of New Mexico, 900 Camino de Salud, NE, Albuquerque, NM 87131.</note>
<affiliation>Present address: School of Medicine, Department of Cell Biology, University of New Mexico, 900 Camino de Salud, NE, Albuquerque, NM 87131.</affiliation>
<affiliation>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</affiliation>
</author>
<idno type="istex">D746914D95FF6CB4EF85F36F0EFECC6B535561BF</idno>
<idno type="ark">ark:/67375/6H6-47ND7VWV-7</idno>
<idno type="DOI">10.1016/0042-6822(89)90199-2</idno>
<idno type="PII">0042-6822(89)90199-2</idno>
</analytic>
<monogr><title level="j">Virology</title>
<title level="j" type="abbrev">YVIRO</title>
<idno type="pISSN">0042-6822</idno>
<idno type="PII">S0042-6822(00)X0263-2</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1989"></date>
<biblScope unit="volume">172</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="564">564</biblScope>
<biblScope unit="page" to="572">572</biblScope>
</imprint>
</monogr>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><creation><date>1989</date>
</creation>
<langUsage><language ident="en">en</language>
</langUsage>
<abstract xml:lang="en"><p>Abstract: Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37° appears slow, with uptake occurring over a period of at least 16 hr.</p>
</abstract>
</profileDesc>
<revisionDesc><change when="1989">Published</change>
</revisionDesc>
</teiHeader>
</istex:fulltextTEI>
<json:item><extension>txt</extension>
<original>false</original>
<mimetype>text/plain</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-47ND7VWV-7/fulltext.txt</uri>
</json:item>
</fulltext>
<metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration>
<istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType>
<istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid>
<aid>89901992</aid>
<ce:pii>0042-6822(89)90199-2</ce:pii>
<ce:doi>10.1016/0042-6822(89)90199-2</ce:doi>
<ce:copyright type="unknown" year="1989"></ce:copyright>
</item-info>
<head><ce:title>Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</ce:title>
<ce:author-group><ce:author><ce:given-name>John C.</ce:given-name>
<ce:surname>Pugh</ce:surname>
<ce:cross-ref refid="COR1"><ce:sup>1</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Jesse W.</ce:given-name>
<ce:surname>Summers</ce:surname>
<ce:cross-ref refid="FN1"><ce:sup>2</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:affiliation><ce:textfn>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</ce:textfn>
</ce:affiliation>
<ce:correspondence id="COR1"><ce:label>1</ce:label>
<ce:text>To whom requests for reprints should be addressed at present address: Dept. of Medicine, St. Mary's Hospital Medical School, South Wharf Road, London W2 1 NY, UK.</ce:text>
</ce:correspondence>
<ce:footnote id="FN1"><ce:label>2</ce:label>
<ce:note-para>Present address: School of Medicine, Department of Cell Biology, University of New Mexico, 900 Camino de Salud, NE, Albuquerque, NM 87131.</ce:note-para>
</ce:footnote>
</ce:author-group>
<ce:date-received day="2" month="11" year="1988"></ce:date-received>
<ce:date-accepted day="5" month="6" year="1989"></ce:date-accepted>
<ce:abstract><ce:section-title>Abstract</ce:section-title>
<ce:abstract-sec><ce:simple-para>Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection <ce:italic>in vitro</ce:italic>
. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37° appears slow, with uptake occurring over a period of at least 16 hr.</ce:simple-para>
</ce:abstract-sec>
</ce:abstract>
</head>
</converted-article>
</istex:document>
</istex:metadataXml>
<mods version="3.6"><titleInfo><title>Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</title>
</titleInfo>
<titleInfo type="alternative" contentType="CDATA"><title>Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide</title>
</titleInfo>
<name type="personal"><namePart type="given">John C.</namePart>
<namePart type="family">Pugh</namePart>
<affiliation>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</affiliation>
<description>To whom requests for reprints should be addressed at present address: Dept. of Medicine, St. Mary's Hospital Medical School, South Wharf Road, London W2 1 NY, UK.</description>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Jesse W.</namePart>
<namePart type="family">Summers</namePart>
<affiliation>Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA</affiliation>
<description>Present address: School of Medicine, Department of Cell Biology, University of New Mexico, 900 Camino de Salud, NE, Albuquerque, NM 87131.</description>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
<genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre>
<originInfo><publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1989</dateIssued>
<copyrightDate encoding="w3cdtf">1989</copyrightDate>
</originInfo>
<language><languageTerm type="code" authority="iso639-2b">eng</languageTerm>
<languageTerm type="code" authority="rfc3066">en</languageTerm>
</language>
<abstract lang="en">Abstract: Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37°. We have indentified a fraction of infecting virus which binds tightly to cells at 4°, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37° appears slow, with uptake occurring over a period of at least 16 hr.</abstract>
<relatedItem type="host"><titleInfo><title>Virology</title>
</titleInfo>
<titleInfo type="abbreviated"><title>YVIRO</title>
</titleInfo>
<genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre>
<originInfo><publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1989</dateIssued>
</originInfo>
<identifier type="ISSN">0042-6822</identifier>
<identifier type="PII">S0042-6822(00)X0263-2</identifier>
<part><date>1989</date>
<detail type="volume"><number>172</number>
<caption>vol.</caption>
</detail>
<detail type="issue"><number>2</number>
<caption>no.</caption>
</detail>
<extent unit="issue-pages"><start>399</start>
<end>679</end>
</extent>
<extent unit="pages"><start>564</start>
<end>572</end>
</extent>
</part>
</relatedItem>
<identifier type="istex">D746914D95FF6CB4EF85F36F0EFECC6B535561BF</identifier>
<identifier type="ark">ark:/67375/6H6-47ND7VWV-7</identifier>
<identifier type="DOI">10.1016/0042-6822(89)90199-2</identifier>
<identifier type="PII">0042-6822(89)90199-2</identifier>
<recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource>
</recordInfo>
</mods>
<json:item><extension>json</extension>
<original>false</original>
<mimetype>application/json</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-47ND7VWV-7/record.json</uri>
</json:item>
</metadata>
</istex>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001714 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001714 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Istex |étape= Corpus |type= RBID |clé= ISTEX:D746914D95FF6CB4EF85F36F0EFECC6B535561BF |texte= Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |