Inhibition of expression of a mouse alpha-globin gene by plasmids that include antisense oligonucleotides.
Identifieur interne : 004724 ( Main/Exploration ); précédent : 004723; suivant : 004725Inhibition of expression of a mouse alpha-globin gene by plasmids that include antisense oligonucleotides.
Auteurs : K. Yokoyama [Japon] ; D X Hou ; H. Gao ; X. Tang ; I. Kitabayashi ; K. Nishikura ; G. GachelinSource :
- Cell structure and function [ 0386-7196 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN recombiné (génétique), ARN antisens (pharmacologie), ARN messager (biosynthèse), ARN messager (génétique), Acétamides (pharmacologie), Animaux, Cellules cancéreuses en culture, Coiffes des ARN (génétique), Différenciation cellulaire (), Données de séquences moléculaires, Globines (biosynthèse), Globines (génétique), Gènes myc, Leucémie érythroblastique aigüe, Modèles génétiques, Plasmides, Régulation de l'expression des gènes (), Souris, Séquence nucléotidique, Séquences d'acides nucléiques régulatrices.
- MESH :
- biosynthèse : ARN messager, Globines.
- génétique : ADN recombiné, ARN messager, Coiffes des ARN, Globines.
- pharmacologie : ARN antisens, Acétamides.
- Animaux, Cellules cancéreuses en culture, Différenciation cellulaire, Données de séquences moléculaires, Gènes myc, Leucémie érythroblastique aigüe, Modèles génétiques, Plasmides, Régulation de l'expression des gènes, Souris, Séquence nucléotidique, Séquences d'acides nucléiques régulatrices.
English descriptors
- KwdEn :
- Acetamides (pharmacology), Animals, Base Sequence, Cell Differentiation (drug effects), DNA, Recombinant (genetics), Gene Expression Regulation (drug effects), Genes, myc, Globins (biosynthesis), Globins (genetics), Leukemia, Erythroblastic, Acute, Mice, Models, Genetic, Molecular Sequence Data, Plasmids, RNA Caps (genetics), RNA, Antisense (pharmacology), RNA, Messenger (biosynthesis), RNA, Messenger (genetics), Regulatory Sequences, Nucleic Acid, Tumor Cells, Cultured.
- MESH :
- chemical , biosynthesis : Globins, RNA, Messenger.
- chemical , genetics : DNA, Recombinant, Globins, RNA Caps, RNA, Messenger.
- chemical , pharmacology : Acetamides, RNA, Antisense.
- drug effects : Cell Differentiation, Gene Expression Regulation.
- Animals, Base Sequence, Genes, myc, Leukemia, Erythroblastic, Acute, Mice, Models, Genetic, Molecular Sequence Data, Plasmids, Regulatory Sequences, Nucleic Acid, Tumor Cells, Cultured.
Abstract
Plasmid-borne DNAs, corresponding to 68-base oligodeoxynucleotides, synthesized in the antisense or sense configuration and based on the nucleotide sequences of various regions of the mouse alpha-globin mRNA, were introduced with the gene for xanthine-guanine phosphoribosyl transferase from E. coli (Ecogpt) into mouse erythroleukemia (MEL) cells by protoplast fusion. Specific inhibition of the synthesis of alpha-globin was observed only in the cells transformed with the plasmids with antisense 68-mers that corresponded to the cap site as well as the site of initiation of translation of alpha-globin mRNA (Oligo-A); Other plasmids with antisense 68-mers that corresponded to the regions of the exon/intron junctions, the individual exons, or the 3' untranslated region were ineffective. This antisense RNA efficiently reduced the production of alpha-globin to 9-18% of the endogenous level after induction with hexylmethylene-bis-acetoamide (HMBA). Moreover, most of the antisense transformants did not show any decrease in the expression of the c-myc gene at the early phases of differentiation of MEL cells. Thus, we propose a hypothesis that the early decline in levels of c-myc mRNA may be independent of and uncoupled from the program of globin synthesis during the differentiation of MEL cells.
DOI: 10.1247/csf.17.433
PubMed: 1295698
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002938
- to stream PubMed, to step Curation: 002938
- to stream PubMed, to step Checkpoint: 002804
- to stream Ncbi, to step Merge: 000214
- to stream Ncbi, to step Curation: 000214
- to stream Ncbi, to step Checkpoint: 000214
- to stream Main, to step Merge: 004794
- to stream Main, to step Curation: 004724
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibition of expression of a mouse alpha-globin gene by plasmids that include antisense oligonucleotides.</title><author><name sortKey="Yokoyama, K" sort="Yokoyama, K" uniqKey="Yokoyama K" first="K" last="Yokoyama">K. Yokoyama</name><affiliation wicri:level="1"><nlm:affiliation>Gene Bank, Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical Research), Ibaraki, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Gene Bank, Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical Research), Ibaraki</wicri:regionArea><wicri:noRegion>Ibaraki</wicri:noRegion></affiliation></author><author><name sortKey="Hou, D X" sort="Hou, D X" uniqKey="Hou D" first="D X" last="Hou">D X Hou</name></author><author><name sortKey="Gao, H" sort="Gao, H" uniqKey="Gao H" first="H" last="Gao">H. Gao</name></author><author><name sortKey="Tang, X" sort="Tang, X" uniqKey="Tang X" first="X" last="Tang">X. Tang</name></author><author><name sortKey="Kitabayashi, I" sort="Kitabayashi, I" uniqKey="Kitabayashi I" first="I" last="Kitabayashi">I. Kitabayashi</name></author><author><name sortKey="Nishikura, K" sort="Nishikura, K" uniqKey="Nishikura K" first="K" last="Nishikura">K. Nishikura</name></author><author><name sortKey="Gachelin, G" sort="Gachelin, G" uniqKey="Gachelin G" first="G" last="Gachelin">G. Gachelin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1295698</idno><idno type="pmid">1295698</idno><idno type="doi">10.1247/csf.17.433</idno><idno type="wicri:Area/PubMed/Corpus">002938</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002938</idno><idno type="wicri:Area/PubMed/Curation">002938</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002938</idno><idno type="wicri:Area/PubMed/Checkpoint">002804</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002804</idno><idno type="wicri:Area/Ncbi/Merge">000214</idno><idno type="wicri:Area/Ncbi/Curation">000214</idno><idno type="wicri:Area/Ncbi/Checkpoint">000214</idno><idno type="wicri:doubleKey">0386-7196:1992:Yokoyama K:inhibition:of:expression</idno><idno type="wicri:Area/Main/Merge">004794</idno><idno type="wicri:Area/Main/Curation">004724</idno><idno type="wicri:Area/Main/Exploration">004724</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Inhibition of expression of a mouse alpha-globin gene by plasmids that include antisense oligonucleotides.</title><author><name sortKey="Yokoyama, K" sort="Yokoyama, K" uniqKey="Yokoyama K" first="K" last="Yokoyama">K. Yokoyama</name><affiliation wicri:level="1"><nlm:affiliation>Gene Bank, Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical Research), Ibaraki, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Gene Bank, Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical Research), Ibaraki</wicri:regionArea><wicri:noRegion>Ibaraki</wicri:noRegion></affiliation></author><author><name sortKey="Hou, D X" sort="Hou, D X" uniqKey="Hou D" first="D X" last="Hou">D X Hou</name></author><author><name sortKey="Gao, H" sort="Gao, H" uniqKey="Gao H" first="H" last="Gao">H. Gao</name></author><author><name sortKey="Tang, X" sort="Tang, X" uniqKey="Tang X" first="X" last="Tang">X. Tang</name></author><author><name sortKey="Kitabayashi, I" sort="Kitabayashi, I" uniqKey="Kitabayashi I" first="I" last="Kitabayashi">I. Kitabayashi</name></author><author><name sortKey="Nishikura, K" sort="Nishikura, K" uniqKey="Nishikura K" first="K" last="Nishikura">K. Nishikura</name></author><author><name sortKey="Gachelin, G" sort="Gachelin, G" uniqKey="Gachelin G" first="G" last="Gachelin">G. Gachelin</name></author></analytic><series><title level="j">Cell structure and function</title><idno type="ISSN">0386-7196</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetamides (pharmacology)</term><term>Animals</term><term>Base Sequence</term><term>Cell Differentiation (drug effects)</term><term>DNA, Recombinant (genetics)</term><term>Gene Expression Regulation (drug effects)</term><term>Genes, myc</term><term>Globins (biosynthesis)</term><term>Globins (genetics)</term><term>Leukemia, Erythroblastic, Acute</term><term>Mice</term><term>Models, Genetic</term><term>Molecular Sequence Data</term><term>Plasmids</term><term>RNA Caps (genetics)</term><term>RNA, Antisense (pharmacology)</term><term>RNA, Messenger (biosynthesis)</term><term>RNA, Messenger (genetics)</term><term>Regulatory Sequences, Nucleic Acid</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN recombiné (génétique)</term><term>ARN antisens (pharmacologie)</term><term>ARN messager (biosynthèse)</term><term>ARN messager (génétique)</term><term>Acétamides (pharmacologie)</term><term>Animaux</term><term>Cellules cancéreuses en culture</term><term>Coiffes des ARN (génétique)</term><term>Différenciation cellulaire ()</term><term>Données de séquences moléculaires</term><term>Globines (biosynthèse)</term><term>Globines (génétique)</term><term>Gènes myc</term><term>Leucémie érythroblastique aigüe</term><term>Modèles génétiques</term><term>Plasmides</term><term>Régulation de l'expression des gènes ()</term><term>Souris</term><term>Séquence nucléotidique</term><term>Séquences d'acides nucléiques régulatrices</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Globins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Recombinant</term><term>Globins</term><term>RNA Caps</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Acetamides</term><term>RNA, Antisense</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN messager</term><term>Globines</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Differentiation</term><term>Gene Expression Regulation</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN recombiné</term><term>ARN messager</term><term>Coiffes des ARN</term><term>Globines</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>ARN antisens</term><term>Acétamides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Genes, myc</term><term>Leukemia, Erythroblastic, Acute</term><term>Mice</term><term>Models, Genetic</term><term>Molecular Sequence Data</term><term>Plasmids</term><term>Regulatory Sequences, Nucleic Acid</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cancéreuses en culture</term><term>Différenciation cellulaire</term><term>Données de séquences moléculaires</term><term>Gènes myc</term><term>Leucémie érythroblastique aigüe</term><term>Modèles génétiques</term><term>Plasmides</term><term>Régulation de l'expression des gènes</term><term>Souris</term><term>Séquence nucléotidique</term><term>Séquences d'acides nucléiques régulatrices</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plasmid-borne DNAs, corresponding to 68-base oligodeoxynucleotides, synthesized in the antisense or sense configuration and based on the nucleotide sequences of various regions of the mouse alpha-globin mRNA, were introduced with the gene for xanthine-guanine phosphoribosyl transferase from E. coli (Ecogpt) into mouse erythroleukemia (MEL) cells by protoplast fusion. Specific inhibition of the synthesis of alpha-globin was observed only in the cells transformed with the plasmids with antisense 68-mers that corresponded to the cap site as well as the site of initiation of translation of alpha-globin mRNA (Oligo-A); Other plasmids with antisense 68-mers that corresponded to the regions of the exon/intron junctions, the individual exons, or the 3' untranslated region were ineffective. This antisense RNA efficiently reduced the production of alpha-globin to 9-18% of the endogenous level after induction with hexylmethylene-bis-acetoamide (HMBA). Moreover, most of the antisense transformants did not show any decrease in the expression of the c-myc gene at the early phases of differentiation of MEL cells. Thus, we propose a hypothesis that the early decline in levels of c-myc mRNA may be independent of and uncoupled from the program of globin synthesis during the differentiation of MEL cells.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Gachelin, G" sort="Gachelin, G" uniqKey="Gachelin G" first="G" last="Gachelin">G. Gachelin</name><name sortKey="Gao, H" sort="Gao, H" uniqKey="Gao H" first="H" last="Gao">H. Gao</name><name sortKey="Hou, D X" sort="Hou, D X" uniqKey="Hou D" first="D X" last="Hou">D X Hou</name><name sortKey="Kitabayashi, I" sort="Kitabayashi, I" uniqKey="Kitabayashi I" first="I" last="Kitabayashi">I. Kitabayashi</name><name sortKey="Nishikura, K" sort="Nishikura, K" uniqKey="Nishikura K" first="K" last="Nishikura">K. Nishikura</name><name sortKey="Tang, X" sort="Tang, X" uniqKey="Tang X" first="X" last="Tang">X. Tang</name></noCountry><country name="Japon"><noRegion><name sortKey="Yokoyama, K" sort="Yokoyama, K" uniqKey="Yokoyama K" first="K" last="Yokoyama">K. Yokoyama</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004724 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004724 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:1295698
|texte= Inhibition of expression of a mouse alpha-globin gene by plasmids that include antisense oligonucleotides.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:1295698" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |