High-performance liquid chromatography and capillary gel electrophoresis as applied to antisense DNA
Identifieur interne : 004576 ( Main/Exploration ); précédent : 004575; suivant : 004577High-performance liquid chromatography and capillary gel electrophoresis as applied to antisense DNA
Auteurs : Aharon S. Cohen [États-Unis] ; Maria Vilenchik [États-Unis] ; Judith L. Dudley [États-Unis] ; Mark W. Gemborys [États-Unis] ; André J. Bourque [États-Unis]Source :
- Journal of Chromatography A [ 0021-9673 ] ; 1993.
English descriptors
- Teeft :
- Analogue, Antisense, Antisense pharmaceuticals, Artificial mixture, Base length, Base sequence, Chromatogr, Cohen, Effective length, Electric field, Electrophoretic methods, Experimental conditions, Failure sequence, Failure sequences, Formic acid, Hpce, Hpce separation, Hplc, Laser densitometer, Liquid chromatography, Migration time, Mobile phase, Mobile phases, Nucleic acids, Oligomers, Oxygen atom, Peak numbers, Power supply, Rapid separation, Retention times, Sequencing, Sodn, Sodn analogues, Sodns, Total length.
Abstract
Abstract: Reversed-phase and ion-exchange HPLC are compared with both slab gel and capillary gel electrophoresis for the separation of antisense phosphorothioate oligomers. The chromatographic separations were found to be markedly inferior to the electrophoretic separations, especially for oligomers greater than 20 bases in length. The potential of gel high-performance capillary electrophoresis for the analysis of phosphorothioate analogues is shown.
Url:
DOI: 10.1016/0021-9673(93)83441-T
Affiliations:
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Le document en format XML
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<term>Base sequence</term>
<term>Chromatogr</term>
<term>Cohen</term>
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<term>Retention times</term>
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<front><div type="abstract" xml:lang="en">Abstract: Reversed-phase and ion-exchange HPLC are compared with both slab gel and capillary gel electrophoresis for the separation of antisense phosphorothioate oligomers. The chromatographic separations were found to be markedly inferior to the electrophoretic separations, especially for oligomers greater than 20 bases in length. The potential of gel high-performance capillary electrophoresis for the analysis of phosphorothioate analogues is shown.</div>
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