Selection of primers for polymerase chain reaction
Identifieur interne : 004138 ( Main/Exploration ); précédent : 004137; suivant : 004139Selection of primers for polymerase chain reaction
Auteurs : Wojciech Rychlik [États-Unis]Source :
- Molecular Biotechnology [ 1073-6085 ] ; 1995-04-01.
English descriptors
- KwdEn :
- Teeft :
- Amplification, Annealing temperature, Annealing time, Base pairs, Degenerate primers, Dimer formation, Duplex, Duplex formation, Duplex stability, Efficient method, Efficient primers, Free energy, General rules, Hairpin loop, High fidelity, Human genomic, Important factors, Nonspecific product formation, Nucleic acids, Nucleotide, Nucleotide concentration, Nucleotide pairs, Polymerase, Polymerase chain reaction, Primer, Primer design, Reaction mixture, Recognition site, Restriction site, Sequence information, Serious problems, Specific primers.
Abstract
Abstract: One of the most important factors affecting the quality of PCR is the choice of primers. In general, the longer the PCR product the more difficult it is to select efficient primers and set appropriate designing primers, and in general, the more DNA sequence information is available, the better the ch0ance of finding an optimal primer pair. Efficient primers can be designed by avoiding the following flaws: primer-dimer formation, self-complementarity, too lowT m of the primers, and/or their incorrect internal stability profile. Tips on subcloning PCR products, calculating duplex stability (predicting dimer formation strength), and designing degenerate primers are given.
Url:
DOI: 10.1007/BF02789108
Affiliations:
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Le document en format XML
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