Selection of primers for polymerase chain reaction
Identifieur interne : 000358 ( Istex/Corpus ); précédent : 000357; suivant : 000359Selection of primers for polymerase chain reaction
Auteurs : Wojciech RychlikSource :
- Molecular Biotechnology [ 1073-6085 ] ; 1995-04-01.
English descriptors
- KwdEn :
- Teeft :
- Amplification, Annealing temperature, Annealing time, Base pairs, Degenerate primers, Dimer formation, Duplex, Duplex formation, Duplex stability, Efficient method, Efficient primers, Free energy, General rules, Hairpin loop, High fidelity, Human genomic, Important factors, Nonspecific product formation, Nucleic acids, Nucleotide, Nucleotide concentration, Nucleotide pairs, Polymerase, Polymerase chain reaction, Primer, Primer design, Reaction mixture, Recognition site, Restriction site, Sequence information, Serious problems, Specific primers.
Abstract
Abstract: One of the most important factors affecting the quality of PCR is the choice of primers. In general, the longer the PCR product the more difficult it is to select efficient primers and set appropriate designing primers, and in general, the more DNA sequence information is available, the better the ch0ance of finding an optimal primer pair. Efficient primers can be designed by avoiding the following flaws: primer-dimer formation, self-complementarity, too lowT m of the primers, and/or their incorrect internal stability profile. Tips on subcloning PCR products, calculating duplex stability (predicting dimer formation strength), and designing degenerate primers are given.
Url:
DOI: 10.1007/BF02789108
Links to Exploration step
ISTEX:A74AD32CC3269F561ECA80818222B4E34740D72ELe document en format XML
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In general, the longer the PCR product the more difficult it is to select efficient primers and set appropriate designing primers, and in general, the more DNA sequence information is available, the better the ch0ance of finding an optimal primer pair. Efficient primers can be designed by avoiding the following flaws: primer-dimer formation, self-complementarity, too lowT m of the primers, and/or their incorrect internal stability profile. Tips on subcloning PCR products, calculating duplex stability (predicting dimer formation strength), and designing degenerate primers are given.</div></front></TEI><istex><corpusName>springer-journals</corpusName><keywords><teeft><json:string>primer</json:string><json:string>polymerase</json:string><json:string>duplex</json:string><json:string>duplex formation</json:string><json:string>nucleic acids</json:string><json:string>duplex stability</json:string><json:string>nucleotide</json:string><json:string>degenerate primers</json:string><json:string>polymerase chain reaction</json:string><json:string>primer design</json:string><json:string>base pairs</json:string><json:string>free energy</json:string><json:string>general rules</json:string><json:string>sequence information</json:string><json:string>reaction mixture</json:string><json:string>high fidelity</json:string><json:string>important factors</json:string><json:string>nucleotide pairs</json:string><json:string>efficient primers</json:string><json:string>serious problems</json:string><json:string>annealing temperature</json:string><json:string>hairpin loop</json:string><json:string>human genomic</json:string><json:string>specific primers</json:string><json:string>annealing time</json:string><json:string>nonspecific product formation</json:string><json:string>nucleotide concentration</json:string><json:string>restriction site</json:string><json:string>recognition site</json:string><json:string>efficient method</json:string><json:string>dimer formation</json:string><json:string>amplification</json:string></teeft></keywords><author><json:item><name>Wojciech Rychlik</name><affiliations><json:string>National Biosciences Inc., 3650 Annapolis Lane North, #140, 55447-5434, Plymouth, MN</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>PCR</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>primer design</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>primer internal stability</value></json:item></subject><articleId><json:string>BF02789108</json:string><json:string>Art6</json:string></articleId><arkIstex>ark:/67375/1BB-PNM042LQ-8</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: One of the most important factors affecting the quality of PCR is the choice of primers. 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In general, the longer the PCR product the more difficult it is to select efficient primers and set appropriate designing primers, and in general, the more DNA sequence information is available, the better the ch0ance of finding an optimal primer pair. Efficient primers can be designed by avoiding the following flaws: primer-dimer formation, self-complementarity, too lowT m of the primers, and/or their incorrect internal stability profile. 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In general, the longer the PCR product the more difficult it is to select efficient primers and set appropriate designing primers, and in general, the more DNA sequence information is available, the better the ch0ance of finding an optimal primer pair. Efficient primers can be designed by avoiding the following flaws: primer-dimer formation, self-complementarity, too low<Emphasis Type="Italic">T</Emphasis><Subscript><Emphasis Type="Italic">m</Emphasis></Subscript> of the primers, and/or their incorrect internal stability profile. Tips on subcloning PCR products, calculating duplex stability (predicting dimer formation strength), and designing degenerate primers are given.</Para></Abstract><KeywordGroup Language="En"><Heading>Index entries</Heading><Keyword>PCR</Keyword><Keyword>primer design</Keyword><Keyword>primer internal stability</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Selection of primers for polymerase chain reaction</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Selection of primers for polymerase chain reaction</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Wojciech</namePart><namePart type="family">Rychlik</namePart><affiliation>National Biosciences Inc., 3650 Annapolis Lane North, #140, 55447-5434, Plymouth, MN</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Humana Press</publisher><place><placeTerm type="text">Totowa</placeTerm></place><dateIssued encoding="w3cdtf">1995-04-01</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: One of the most important factors affecting the quality of PCR is the choice of primers. In general, the longer the PCR product the more difficult it is to select efficient primers and set appropriate designing primers, and in general, the more DNA sequence information is available, the better the ch0ance of finding an optimal primer pair. Efficient primers can be designed by avoiding the following flaws: primer-dimer formation, self-complementarity, too lowT m of the primers, and/or their incorrect internal stability profile. 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