MersV1, Main, Exploration, bibRecord, 003785

Sequence specific hybridization properties of methylphosphonate oligodeoxynucleotides.

Identifieur interne : 003785 ( Main/Exploration ); précédent : 003784; suivant : 003786

Sequence specific hybridization properties of methylphosphonate oligodeoxynucleotides.

Auteurs : M. Schweitzer [Allemagne] ; J W Engels

Source :

Journal of biomolecular structure & dynamics [ 0739-1102 ] ; 1999.

RBID : pubmed:10447202

Descripteurs français

KwdFr :
- Composés organiques du phosphore (), Conformation d'acide nucléique, Hybridation d'acides nucléiques, Hétéroduplexes d'acides nucléiques, Oligodésoxyribonucléotides (), Électricité statique.
MESH :
- Composés organiques du phosphore, Conformation d'acide nucléique, Hybridation d'acides nucléiques, Hétéroduplexes d'acides nucléiques, Oligodésoxyribonucléotides, Électricité statique.

English descriptors

KwdEn :
- Nucleic Acid Conformation, Nucleic Acid Heteroduplexes, Nucleic Acid Hybridization, Oligodeoxyribonucleotides (chemistry), Organophosphorus Compounds (chemistry), Static Electricity.
MESH :
- chemical , chemistry : Oligodeoxyribonucleotides, Organophosphorus Compounds.
- chemical : Nucleic Acid Heteroduplexes.
- Nucleic Acid Conformation, Nucleic Acid Hybridization, Static Electricity.

Abstract

Methylphosphonate oligodeoxynucleotides (MPO's) with isomerically pure Rp-configurated methylphosphonates (MP's) were synthesized by block coupling of ApT and TpA dinucleoside methylphosphonates (DMP's, p indicating MP-linkage). Oligonucleotide duplexes (20 mers) with these Rp-MP's showed almost the same melting temperatures (Tm) as those with phosphorodiester bonds. Further a dependence of the duplex stability from the nucleosides (bases) adjacent to the MP moiety was observed. For the first time thermodynamic parameters for the duplex to coil transition of isomerically pure MP's were determined from the concentration dependence of the Tm. CD-spectra of the duplexes show structural changes which can be associated with the transition to a compact helix with higher helix winding angle.

DOI: 10.1080/07391102.1999.10508326
PubMed: 10447202

Affiliations:

Le document en format XML

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<term>Oligodésoxyribonucléotides ()</term>
<term>Électricité statique</term>
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<term>Nucleic Acid Hybridization</term>
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<term>Conformation d'acide nucléique</term>
<term>Hybridation d'acides nucléiques</term>
<term>Hétéroduplexes d'acides nucléiques</term>
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<front><div type="abstract" xml:lang="en">Methylphosphonate oligodeoxynucleotides (MPO's) with isomerically pure Rp-configurated methylphosphonates (MP's) were synthesized by block coupling of ApT and TpA dinucleoside methylphosphonates (DMP's, p indicating MP-linkage). Oligonucleotide duplexes (20 mers) with these Rp-MP's showed almost the same melting temperatures (Tm) as those with phosphorodiester bonds. Further a dependence of the duplex stability from the nucleosides (bases) adjacent to the MP moiety was observed. For the first time thermodynamic parameters for the duplex to coil transition of isomerically pure MP's were determined from the concentration dependence of the Tm. CD-spectra of the duplexes show structural changes which can be associated with the transition to a compact helix with higher helix winding angle.</div>
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Serveur d'exploration MERS

Sequence specific hybridization properties of methylphosphonate oligodeoxynucleotides.

Sequence specific hybridization properties of methylphosphonate oligodeoxynucleotides.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
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