Sequence specific hybridization properties of methylphosphonate oligodeoxynucleotides.
Identifieur interne : 003785 ( Main/Curation ); précédent : 003784; suivant : 003786Sequence specific hybridization properties of methylphosphonate oligodeoxynucleotides.
Auteurs : M. Schweitzer [Allemagne] ; J W EngelsSource :
- Journal of biomolecular structure & dynamics [ 0739-1102 ] ; 1999.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Oligodeoxyribonucleotides, Organophosphorus Compounds.
- chemical : Nucleic Acid Heteroduplexes.
- Nucleic Acid Conformation, Nucleic Acid Hybridization, Static Electricity.
Abstract
Methylphosphonate oligodeoxynucleotides (MPO's) with isomerically pure Rp-configurated methylphosphonates (MP's) were synthesized by block coupling of ApT and TpA dinucleoside methylphosphonates (DMP's, p indicating MP-linkage). Oligonucleotide duplexes (20 mers) with these Rp-MP's showed almost the same melting temperatures (Tm) as those with phosphorodiester bonds. Further a dependence of the duplex stability from the nucleosides (bases) adjacent to the MP moiety was observed. For the first time thermodynamic parameters for the duplex to coil transition of isomerically pure MP's were determined from the concentration dependence of the Tm. CD-spectra of the duplexes show structural changes which can be associated with the transition to a compact helix with higher helix winding angle.
DOI: 10.1080/07391102.1999.10508326
PubMed: 10447202
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<term>Organophosphorus Compounds (chemistry)</term>
<term>Static Electricity</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Composés organiques du phosphore ()</term>
<term>Conformation d'acide nucléique</term>
<term>Hybridation d'acides nucléiques</term>
<term>Hétéroduplexes d'acides nucléiques</term>
<term>Oligodésoxyribonucléotides ()</term>
<term>Électricité statique</term>
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<term>Organophosphorus Compounds</term>
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<term>Hybridation d'acides nucléiques</term>
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<front><div type="abstract" xml:lang="en">Methylphosphonate oligodeoxynucleotides (MPO's) with isomerically pure Rp-configurated methylphosphonates (MP's) were synthesized by block coupling of ApT and TpA dinucleoside methylphosphonates (DMP's, p indicating MP-linkage). Oligonucleotide duplexes (20 mers) with these Rp-MP's showed almost the same melting temperatures (Tm) as those with phosphorodiester bonds. Further a dependence of the duplex stability from the nucleosides (bases) adjacent to the MP moiety was observed. For the first time thermodynamic parameters for the duplex to coil transition of isomerically pure MP's were determined from the concentration dependence of the Tm. CD-spectra of the duplexes show structural changes which can be associated with the transition to a compact helix with higher helix winding angle.</div>
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