Subunit sequences of the 4 x 6-mer hemocyanin from the golden orb-web spider, Nephila inaurata.
Identifieur interne : 003194 ( Main/Exploration ); précédent : 003193; suivant : 003195Subunit sequences of the 4 x 6-mer hemocyanin from the golden orb-web spider, Nephila inaurata.
Auteurs : Anne Averdam [Allemagne] ; Jürgen Markl ; Thorsten BurmesterSource :
- European journal of biochemistry [ 0014-2956 ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Hemocyanins.
- chemical , genetics : Hemocyanins.
- chemical , metabolism : Hemocyanins.
- chemistry : Spiders.
- genetics : Spiders.
- Amino Acid Sequence, Animals, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Evolution, Molecular, Immunoelectrophoresis, Molecular Sequence Data, Phylogeny.
Abstract
The transport of oxygen in the hemolymph of many arthropod and mollusc species is mediated by large copper-proteins that are referred to as hemocyanins. Arthropod hemocyanins are composed of hexamers and oligomers of hexamers. Arachnid hemocyanins usually form 4 x 6-mers consisting of seven distinct subunit types (termed a-g), although in some spider taxa deviations from this standard scheme have been observed. Applying immunological and electrophoretic methods, six distinct hemocyanin subunits were identified in the red-legged golden orb-web spider Nephila inaurata madagascariensis (Araneae: Tetragnathidae). The complete cDNA sequences of six subunits were obtained that corresponded to a-, b-, d-, e-, f- and g-type subunits. No evidence for a c-type subunit was found in this species. The inclusion of the N. inaurata hemocyanins in a multiple alignment of the arthropod hemocyanins and the application of the Bayesian method of phylogenetic inference allow, for the first time, a solid reconstruction of the intramolecular evolution of the chelicerate hemocyanin subunits. The branch leading to subunit a diverged first, followed by the common branch of the dimer-forming b and c subunits, while subunits d and f, as well as subunits e and g form common branches. Assuming a clock-like evolution of the chelicerate hemocyanins, a timescale for the evolution of the Chelicerata was obtained that agrees with the fossil record.
DOI: 10.1046/j.1432-1033.2003.03730.x
PubMed: 12899700
Affiliations:
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sort="Burmester, Thorsten" uniqKey="Burmester T" first="Thorsten" last="Burmester">Thorsten Burmester</name></author></analytic><series><title level="j">European journal of biochemistry</title><idno type="ISSN">0014-2956</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Blotting, Western</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Evolution, Molecular</term><term>Hemocyanins (chemistry)</term><term>Hemocyanins (genetics)</term><term>Hemocyanins (metabolism)</term><term>Immunoelectrophoresis</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Spiders (chemistry)</term><term>Spiders (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Araignées ()</term><term>Araignées (génétique)</term><term>Données de séquences 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Polyacrylamide Gel</term><term>Evolution, Molecular</term><term>Immunoelectrophoresis</term><term>Molecular Sequence Data</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Araignées</term><term>Données de séquences moléculaires</term><term>Immunoélectrophorèse</term><term>Phylogénie</term><term>Séquence d'acides aminés</term><term>Technique de Western</term><term>Électrophorèse sur gel de polyacrylamide</term><term>Évolution moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The transport of oxygen in the hemolymph of many arthropod and mollusc species is mediated by large copper-proteins that are referred to as hemocyanins. Arthropod hemocyanins are composed of hexamers and oligomers of hexamers. Arachnid hemocyanins usually form 4 x 6-mers consisting of seven distinct subunit types (termed a-g), although in some spider taxa deviations from this standard scheme have been observed. Applying immunological and electrophoretic methods, six distinct hemocyanin subunits were identified in the red-legged golden orb-web spider Nephila inaurata madagascariensis (Araneae: Tetragnathidae). The complete cDNA sequences of six subunits were obtained that corresponded to a-, b-, d-, e-, f- and g-type subunits. No evidence for a c-type subunit was found in this species. The inclusion of the N. inaurata hemocyanins in a multiple alignment of the arthropod hemocyanins and the application of the Bayesian method of phylogenetic inference allow, for the first time, a solid reconstruction of the intramolecular evolution of the chelicerate hemocyanin subunits. The branch leading to subunit a diverged first, followed by the common branch of the dimer-forming b and c subunits, while subunits d and f, as well as subunits e and g form common branches. Assuming a clock-like evolution of the chelicerate hemocyanins, a timescale for the evolution of the Chelicerata was obtained that agrees with the fossil record.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Rhénanie-Palatinat</li></region><settlement><li>Mayence</li></settlement></list><tree><noCountry><name sortKey="Burmester, Thorsten" sort="Burmester, Thorsten" uniqKey="Burmester T" first="Thorsten" last="Burmester">Thorsten Burmester</name><name sortKey="Markl, Jurgen" sort="Markl, Jurgen" uniqKey="Markl J" first="Jürgen" last="Markl">Jürgen Markl</name></noCountry><country name="Allemagne"><region name="Rhénanie-Palatinat"><name sortKey="Averdam, Anne" sort="Averdam, Anne" uniqKey="Averdam A" first="Anne" last="Averdam">Anne Averdam</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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