Activation of natural killer-like YT-INDY cells by oligodeoxynucleotides and binding by homologous pattern recognition proteins.
Identifieur interne : 002F21 ( Main/Exploration ); précédent : 002F20; suivant : 002F22Activation of natural killer-like YT-INDY cells by oligodeoxynucleotides and binding by homologous pattern recognition proteins.
Auteurs : H. Kaur [États-Unis] ; L. Jaso-Friedmann ; J H Leary ; K. Praveen ; Z. Brahmi ; D L EvansSource :
- Scandinavian journal of immunology [ 0300-9475 ] ; 2005.
Descripteurs français
- KwdFr :
- Activation des lymphocytes (), Activation des lymphocytes (immunologie), Adjuvants immunologiques (pharmacologie), Anticorps, Calcium (métabolisme), Cellules tueuses naturelles (), Cellules tueuses naturelles (immunologie), Cellules tueuses naturelles (métabolisme), Histone (immunologie), Humains, Ilots CpG (génétique), Ilots CpG (immunologie), Liaison aux protéines (immunologie), Lignée cellulaire tumorale, Oligodésoxyribonucléotides (immunologie), Oligodésoxyribonucléotides (pharmacologie), Protéines de liaison à l'ADN (métabolisme), Récepteurs de reconnaissance de motifs moléculaires (métabolisme), Technique de Southwestern.
- MESH :
- génétique : Ilots CpG.
- immunologie : Activation des lymphocytes, Cellules tueuses naturelles, Histone, Ilots CpG, Liaison aux protéines, Oligodésoxyribonucléotides.
- métabolisme : Calcium, Cellules tueuses naturelles, Protéines de liaison à l'ADN, Récepteurs de reconnaissance de motifs moléculaires.
- pharmacologie : Adjuvants immunologiques, Oligodésoxyribonucléotides.
- Activation des lymphocytes, Anticorps, Cellules tueuses naturelles, Humains, Lignée cellulaire tumorale, Technique de Southwestern.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (pharmacology), Antibodies, Blotting, Southwestern, Calcium (metabolism), Cell Line, Tumor, CpG Islands (genetics), CpG Islands (immunology), DNA-Binding Proteins (metabolism), Histones (immunology), Humans, Killer Cells, Natural (drug effects), Killer Cells, Natural (immunology), Killer Cells, Natural (metabolism), Lymphocyte Activation (drug effects), Lymphocyte Activation (immunology), Oligodeoxyribonucleotides (immunology), Oligodeoxyribonucleotides (pharmacology), Protein Binding (immunology), Receptors, Pattern Recognition (metabolism).
- MESH :
- chemical , immunology : Histones, Oligodeoxyribonucleotides.
- chemical , metabolism : Calcium, DNA-Binding Proteins, Receptors, Pattern Recognition.
- chemical , pharmacology : Adjuvants, Immunologic, Oligodeoxyribonucleotides.
- chemical : Antibodies.
- drug effects : Killer Cells, Natural, Lymphocyte Activation.
- genetics : CpG Islands.
- immunology : CpG Islands, Killer Cells, Natural, Lymphocyte Activation, Protein Binding.
- metabolism : Killer Cells, Natural.
- Blotting, Southwestern, Cell Line, Tumor, Humans.
Abstract
The present study was designed to examine the binding and signalling effects of single base and CpG dinucleotide phosphodiester (Po) oligodeoxynucleotides (ODN) on the human natural killer (NK)-like cell line (YT-INDY). Single base Po ODN composed of 20-mers of guanosine (dG20), adenosine (dA20), cytosine (dC20) or thymidine (dT20) as well as 'conventional' Po CpG ODN were examined for their ability to bind and activate YT-INDY cells. Binding by dG20 and CpG ODN to YT-INDY cells was saturable and specific. dG20 binding was competitively inhibited by homologous dG20 and heterologous CpG ODN but not by dC20 and dA20. Two different YT-INDY membrane proteins (18 and 29 kDa) were identified by ligand (Southwestern) blotting with biotinylated dG20 and CpG. The specificity of the ODN-binding protein(s) was further confirmed by ODN depletion experiments using a teleost recombinant protein orthologue [nonspecific cytotoxic cells (NCC) cationic antimicrobial protein-1 (ncamp-1)] known to bind CpG and dG20. Cell proliferation and activation studies showed that dG20 and CpG treatment of YT-INDY cells induced cellular DNA synthesis (i.e. G1 to S-phase conversion). This signalling function was accompanied in dG20-treated cells by proliferation 10 h posttreatment. Both dG20 and CpG ODN binding induced a calcium flux in YT-INDY cells within seconds of treatment. These experiments demonstrated that Po single base dG20 and CpG ODN bind to a (potential) new class of cell-surface proteins that mediate the activation of YT-INDY cells.
DOI: 10.1111/j.1365-3083.2005.01665.x
PubMed: 16253123
Affiliations:
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Le document en format XML
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Single base Po ODN composed of 20-mers of guanosine (dG20), adenosine (dA20), cytosine (dC20) or thymidine (dT20) as well as 'conventional' Po CpG ODN were examined for their ability to bind and activate YT-INDY cells. Binding by dG20 and CpG ODN to YT-INDY cells was saturable and specific. dG20 binding was competitively inhibited by homologous dG20 and heterologous CpG ODN but not by dC20 and dA20. Two different YT-INDY membrane proteins (18 and 29 kDa) were identified by ligand (Southwestern) blotting with biotinylated dG20 and CpG. The specificity of the ODN-binding protein(s) was further confirmed by ODN depletion experiments using a teleost recombinant protein orthologue [nonspecific cytotoxic cells (NCC) cationic antimicrobial protein-1 (ncamp-1)] known to bind CpG and dG20. Cell proliferation and activation studies showed that dG20 and CpG treatment of YT-INDY cells induced cellular DNA synthesis (i.e. G1 to S-phase conversion). This signalling function was accompanied in dG20-treated cells by proliferation 10 h posttreatment. Both dG20 and CpG ODN binding induced a calcium flux in YT-INDY cells within seconds of treatment. These experiments demonstrated that Po single base dG20 and CpG ODN bind to a (potential) new class of cell-surface proteins that mediate the activation of YT-INDY cells.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Brahmi, Z" sort="Brahmi, Z" uniqKey="Brahmi Z" first="Z" last="Brahmi">Z. Brahmi</name><name sortKey="Evans, D L" sort="Evans, D L" uniqKey="Evans D" first="D L" last="Evans">D L Evans</name><name sortKey="Jaso Friedmann, L" sort="Jaso Friedmann, L" uniqKey="Jaso Friedmann L" first="L" last="Jaso-Friedmann">L. Jaso-Friedmann</name><name sortKey="Leary, J H" sort="Leary, J H" uniqKey="Leary J" first="J H" last="Leary">J H Leary</name><name sortKey="Praveen, K" sort="Praveen, K" uniqKey="Praveen K" first="K" last="Praveen">K. Praveen</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Kaur, H" sort="Kaur, H" uniqKey="Kaur H" first="H" last="Kaur">H. Kaur</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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