Activation of natural killer-like YT-INDY cells by oligodeoxynucleotides and binding by homologous pattern recognition proteins.
Identifieur interne : 002301 ( PubMed/Corpus ); précédent : 002300; suivant : 002302Activation of natural killer-like YT-INDY cells by oligodeoxynucleotides and binding by homologous pattern recognition proteins.
Auteurs : H. Kaur ; L. Jaso-Friedmann ; J H Leary ; K. Praveen ; Z. Brahmi ; D L EvansSource :
- Scandinavian journal of immunology [ 0300-9475 ] ; 2005.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (pharmacology), Antibodies, Blotting, Southwestern, Calcium (metabolism), Cell Line, Tumor, CpG Islands (genetics), CpG Islands (immunology), DNA-Binding Proteins (metabolism), Histones (immunology), Humans, Killer Cells, Natural (drug effects), Killer Cells, Natural (immunology), Killer Cells, Natural (metabolism), Lymphocyte Activation (drug effects), Lymphocyte Activation (immunology), Oligodeoxyribonucleotides (immunology), Oligodeoxyribonucleotides (pharmacology), Protein Binding (immunology), Receptors, Pattern Recognition (metabolism).
- MESH :
- chemical , immunology : Histones, Oligodeoxyribonucleotides.
- chemical , metabolism : Calcium, DNA-Binding Proteins, Receptors, Pattern Recognition.
- chemical , pharmacology : Adjuvants, Immunologic, Oligodeoxyribonucleotides.
- chemical : Antibodies.
- drug effects : Killer Cells, Natural, Lymphocyte Activation.
- genetics : CpG Islands.
- immunology : CpG Islands, Killer Cells, Natural, Lymphocyte Activation, Protein Binding.
- metabolism : Killer Cells, Natural.
- Blotting, Southwestern, Cell Line, Tumor, Humans.
Abstract
The present study was designed to examine the binding and signalling effects of single base and CpG dinucleotide phosphodiester (Po) oligodeoxynucleotides (ODN) on the human natural killer (NK)-like cell line (YT-INDY). Single base Po ODN composed of 20-mers of guanosine (dG20), adenosine (dA20), cytosine (dC20) or thymidine (dT20) as well as 'conventional' Po CpG ODN were examined for their ability to bind and activate YT-INDY cells. Binding by dG20 and CpG ODN to YT-INDY cells was saturable and specific. dG20 binding was competitively inhibited by homologous dG20 and heterologous CpG ODN but not by dC20 and dA20. Two different YT-INDY membrane proteins (18 and 29 kDa) were identified by ligand (Southwestern) blotting with biotinylated dG20 and CpG. The specificity of the ODN-binding protein(s) was further confirmed by ODN depletion experiments using a teleost recombinant protein orthologue [nonspecific cytotoxic cells (NCC) cationic antimicrobial protein-1 (ncamp-1)] known to bind CpG and dG20. Cell proliferation and activation studies showed that dG20 and CpG treatment of YT-INDY cells induced cellular DNA synthesis (i.e. G1 to S-phase conversion). This signalling function was accompanied in dG20-treated cells by proliferation 10 h posttreatment. Both dG20 and CpG ODN binding induced a calcium flux in YT-INDY cells within seconds of treatment. These experiments demonstrated that Po single base dG20 and CpG ODN bind to a (potential) new class of cell-surface proteins that mediate the activation of YT-INDY cells.
DOI: 10.1111/j.1365-3083.2005.01665.x
PubMed: 16253123
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pubmed:16253123Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Activation of natural killer-like YT-INDY cells by oligodeoxynucleotides and binding by homologous pattern recognition proteins.</title><author><name sortKey="Kaur, H" sort="Kaur, H" uniqKey="Kaur H" first="H" last="Kaur">H. Kaur</name><affiliation><nlm:affiliation>Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, 30602, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Jaso Friedmann, L" sort="Jaso Friedmann, L" uniqKey="Jaso Friedmann L" first="L" last="Jaso-Friedmann">L. Jaso-Friedmann</name></author><author><name sortKey="Leary, J H" sort="Leary, J H" uniqKey="Leary J" first="J H" last="Leary">J H Leary</name></author><author><name sortKey="Praveen, K" sort="Praveen, K" uniqKey="Praveen K" first="K" last="Praveen">K. Praveen</name></author><author><name sortKey="Brahmi, Z" sort="Brahmi, Z" uniqKey="Brahmi Z" first="Z" last="Brahmi">Z. Brahmi</name></author><author><name sortKey="Evans, D L" sort="Evans, D L" uniqKey="Evans D" first="D L" last="Evans">D L Evans</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16253123</idno><idno type="pmid">16253123</idno><idno type="doi">10.1111/j.1365-3083.2005.01665.x</idno><idno type="wicri:Area/PubMed/Corpus">002301</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002301</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Activation of natural killer-like YT-INDY cells by oligodeoxynucleotides and binding by homologous pattern recognition proteins.</title><author><name sortKey="Kaur, H" sort="Kaur, H" uniqKey="Kaur H" first="H" last="Kaur">H. Kaur</name><affiliation><nlm:affiliation>Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, 30602, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Jaso Friedmann, L" sort="Jaso Friedmann, L" uniqKey="Jaso Friedmann L" first="L" last="Jaso-Friedmann">L. Jaso-Friedmann</name></author><author><name sortKey="Leary, J H" sort="Leary, J H" uniqKey="Leary J" first="J H" last="Leary">J H Leary</name></author><author><name sortKey="Praveen, K" sort="Praveen, K" uniqKey="Praveen K" first="K" last="Praveen">K. Praveen</name></author><author><name sortKey="Brahmi, Z" sort="Brahmi, Z" uniqKey="Brahmi Z" first="Z" last="Brahmi">Z. Brahmi</name></author><author><name sortKey="Evans, D L" sort="Evans, D L" uniqKey="Evans D" first="D L" last="Evans">D L Evans</name></author></analytic><series><title level="j">Scandinavian journal of immunology</title><idno type="ISSN">0300-9475</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic (pharmacology)</term><term>Antibodies</term><term>Blotting, Southwestern</term><term>Calcium (metabolism)</term><term>Cell Line, Tumor</term><term>CpG Islands (genetics)</term><term>CpG Islands (immunology)</term><term>DNA-Binding Proteins (metabolism)</term><term>Histones (immunology)</term><term>Humans</term><term>Killer Cells, Natural (drug effects)</term><term>Killer Cells, Natural (immunology)</term><term>Killer Cells, Natural (metabolism)</term><term>Lymphocyte Activation (drug effects)</term><term>Lymphocyte Activation (immunology)</term><term>Oligodeoxyribonucleotides (immunology)</term><term>Oligodeoxyribonucleotides (pharmacology)</term><term>Protein Binding (immunology)</term><term>Receptors, Pattern Recognition (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Histones</term><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term><term>DNA-Binding Proteins</term><term>Receptors, Pattern Recognition</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Adjuvants, Immunologic</term><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Killer Cells, Natural</term><term>Lymphocyte Activation</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>CpG Islands</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CpG Islands</term><term>Killer Cells, Natural</term><term>Lymphocyte Activation</term><term>Protein Binding</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Killer Cells, Natural</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Southwestern</term><term>Cell Line, Tumor</term><term>Humans</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The present study was designed to examine the binding and signalling effects of single base and CpG dinucleotide phosphodiester (Po) oligodeoxynucleotides (ODN) on the human natural killer (NK)-like cell line (YT-INDY). Single base Po ODN composed of 20-mers of guanosine (dG20), adenosine (dA20), cytosine (dC20) or thymidine (dT20) as well as 'conventional' Po CpG ODN were examined for their ability to bind and activate YT-INDY cells. Binding by dG20 and CpG ODN to YT-INDY cells was saturable and specific. dG20 binding was competitively inhibited by homologous dG20 and heterologous CpG ODN but not by dC20 and dA20. Two different YT-INDY membrane proteins (18 and 29 kDa) were identified by ligand (Southwestern) blotting with biotinylated dG20 and CpG. The specificity of the ODN-binding protein(s) was further confirmed by ODN depletion experiments using a teleost recombinant protein orthologue [nonspecific cytotoxic cells (NCC) cationic antimicrobial protein-1 (ncamp-1)] known to bind CpG and dG20. Cell proliferation and activation studies showed that dG20 and CpG treatment of YT-INDY cells induced cellular DNA synthesis (i.e. G1 to S-phase conversion). This signalling function was accompanied in dG20-treated cells by proliferation 10 h posttreatment. Both dG20 and CpG ODN binding induced a calcium flux in YT-INDY cells within seconds of treatment. These experiments demonstrated that Po single base dG20 and CpG ODN bind to a (potential) new class of cell-surface proteins that mediate the activation of YT-INDY cells.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16253123</PMID><DateCompleted><Year>2005</Year><Month>12</Month><Day>07</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0300-9475</ISSN><JournalIssue CitedMedium="Print"><Volume>62</Volume><Issue>4</Issue><PubDate><Year>2005</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Scandinavian journal of immunology</Title><ISOAbbreviation>Scand. J. Immunol.</ISOAbbreviation></Journal><ArticleTitle>Activation of natural killer-like YT-INDY cells by oligodeoxynucleotides and binding by homologous pattern recognition proteins.</ArticleTitle><Pagination><MedlinePgn>361-70</MedlinePgn></Pagination><Abstract><AbstractText>The present study was designed to examine the binding and signalling effects of single base and CpG dinucleotide phosphodiester (Po) oligodeoxynucleotides (ODN) on the human natural killer (NK)-like cell line (YT-INDY). Single base Po ODN composed of 20-mers of guanosine (dG20), adenosine (dA20), cytosine (dC20) or thymidine (dT20) as well as 'conventional' Po CpG ODN were examined for their ability to bind and activate YT-INDY cells. Binding by dG20 and CpG ODN to YT-INDY cells was saturable and specific. dG20 binding was competitively inhibited by homologous dG20 and heterologous CpG ODN but not by dC20 and dA20. Two different YT-INDY membrane proteins (18 and 29 kDa) were identified by ligand (Southwestern) blotting with biotinylated dG20 and CpG. The specificity of the ODN-binding protein(s) was further confirmed by ODN depletion experiments using a teleost recombinant protein orthologue [nonspecific cytotoxic cells (NCC) cationic antimicrobial protein-1 (ncamp-1)] known to bind CpG and dG20. Cell proliferation and activation studies showed that dG20 and CpG treatment of YT-INDY cells induced cellular DNA synthesis (i.e. G1 to S-phase conversion). This signalling function was accompanied in dG20-treated cells by proliferation 10 h posttreatment. Both dG20 and CpG ODN binding induced a calcium flux in YT-INDY cells within seconds of treatment. These experiments demonstrated that Po single base dG20 and CpG ODN bind to a (potential) new class of cell-surface proteins that mediate the activation of YT-INDY cells.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kaur</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, 30602, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jaso-Friedmann</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Leary</LastName><ForeName>J H</ForeName><Initials>JH</Initials><Suffix>3rd</Suffix></Author><Author ValidYN="Y"><LastName>Praveen</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Brahmi</LastName><ForeName>Z</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Evans</LastName><ForeName>D L</ForeName><Initials>DL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Scand J Immunol</MedlineTA><NlmUniqueID>0323767</NlmUniqueID><ISSNLinking>0300-9475</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000276">Adjuvants, Immunologic</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000906">Antibodies</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C408982">CPG-oligonucleotide</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004268">DNA-Binding Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006657">Histones</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009838">Oligodeoxyribonucleotides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D051192">Receptors, Pattern Recognition</NameOfSubstance></Chemical><Chemical><RegistryNumber>SY7Q814VUP</RegistryNumber><NameOfSubstance UI="D002118">Calcium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000276" MajorTopicYN="N">Adjuvants, Immunologic</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000906" MajorTopicYN="N">Antibodies</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D026501" MajorTopicYN="N">Blotting, 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