DNA Microarrays: Applications, Future Trends, and the Need for Standardization
Identifieur interne : 002A96 ( Main/Exploration ); précédent : 002A95; suivant : 002A97DNA Microarrays: Applications, Future Trends, and the Need for Standardization
Auteurs : Sige Zou [États-Unis] ; Hua-Jun He [États-Unis] ; Yaping Zong [États-Unis] ; Leming Shi [États-Unis] ; Lili Wang [États-Unis]Source :
- Springer Series on Fluorescence [ 1617-1306 ]
Abstract
Abstract: Microarray technology allows high-throughput analysis of tens or even hundreds of thousands of genes in a single experiment, and has been applied broadly to address a wide variety of questions in basic and applied sciences. The applications of the microarray technology range from gene-expression profiling and genotyping to DNA–protein interactions and genome sequencing, and the list of applications keeps growing, especially when combined with other technologies such as proteomic technologies. However, many steps are involved in each microarray experiment and a number of microarray platforms exist, each with its unique features. The challenge is how to standardize the methods and materials to allow intra- and inter-comparison of microarray data collected in the same or different set of experiments. With the challenge in mind, we address the need for standardization for each step of the microarray experiments with emphasis on quality control of array fabrication and scanner calibration and verification. The proposed standards are designed for checking the quality of mRNA, fabricating slides, hybridization, and collecting, analyzing, and storing data. By implementing standards for each step of the microarray process, the full potential of the microarray technology will be realized, especially in the area of disease diagnosis and drug development.
Url:
DOI: 10.1007/4243_2008_036
Affiliations:
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The applications of the microarray technology range from gene-expression profiling and genotyping to DNA–protein interactions and genome sequencing, and the list of applications keeps growing, especially when combined with other technologies such as proteomic technologies. However, many steps are involved in each microarray experiment and a number of microarray platforms exist, each with its unique features. The challenge is how to standardize the methods and materials to allow intra- and inter-comparison of microarray data collected in the same or different set of experiments. With the challenge in mind, we address the need for standardization for each step of the microarray experiments with emphasis on quality control of array fabrication and scanner calibration and verification. The proposed standards are designed for checking the quality of mRNA, fabricating slides, hybridization, and collecting, analyzing, and storing data. 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