DNA Microarrays: Applications, Future Trends, and the Need for Standardization
Identifieur interne : 000B14 ( Istex/Corpus ); précédent : 000B13; suivant : 000B15DNA Microarrays: Applications, Future Trends, and the Need for Standardization
Auteurs : Sige Zou ; Hua-Jun He ; Yaping Zong ; Leming Shi ; Lili WangSource :
- Springer Series on Fluorescence [ 1617-1306 ]
Abstract
Abstract: Microarray technology allows high-throughput analysis of tens or even hundreds of thousands of genes in a single experiment, and has been applied broadly to address a wide variety of questions in basic and applied sciences. The applications of the microarray technology range from gene-expression profiling and genotyping to DNA–protein interactions and genome sequencing, and the list of applications keeps growing, especially when combined with other technologies such as proteomic technologies. However, many steps are involved in each microarray experiment and a number of microarray platforms exist, each with its unique features. The challenge is how to standardize the methods and materials to allow intra- and inter-comparison of microarray data collected in the same or different set of experiments. With the challenge in mind, we address the need for standardization for each step of the microarray experiments with emphasis on quality control of array fabrication and scanner calibration and verification. The proposed standards are designed for checking the quality of mRNA, fabricating slides, hybridization, and collecting, analyzing, and storing data. By implementing standards for each step of the microarray process, the full potential of the microarray technology will be realized, especially in the area of disease diagnosis and drug development.
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DOI: 10.1007/4243_2008_036
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The applications of the microarray technology range from gene-expression profiling and genotyping to DNA–protein interactions and genome sequencing, and the list of applications keeps growing, especially when combined with other technologies such as proteomic technologies. However, many steps are involved in each microarray experiment and a number of microarray platforms exist, each with its unique features. The challenge is how to standardize the methods and materials to allow intra- and inter-comparison of microarray data collected in the same or different set of experiments. With the challenge in mind, we address the need for standardization for each step of the microarray experiments with emphasis on quality control of array fabrication and scanner calibration and verification. The proposed standards are designed for checking the quality of mRNA, fabricating slides, hybridization, and collecting, analyzing, and storing data. By implementing standards for each step of the microarray process, the full potential of the microarray technology will be realized, especially in the area of disease diagnosis and drug development.</div></front></TEI><istex><corpusName>springer-ebooks</corpusName><author><json:item><name>Sige Zou</name><affiliations><json:string>Laboratory of Experimental Gerontology, National Institute on Aging, National Institutes of Health (NIH), 5600 Nathan Shock Drive, 21224, Baltimore, MD, USA</json:string></affiliations></json:item><json:item><name>Hua-Jun He</name><affiliations><json:string>Biochemical Science Division, National Institute of Standards and Technology (NIST), 100 Bureau Drive MS 8312, 20899-8312, Gaithersburg, Maryland, USA</json:string></affiliations></json:item><json:item><name>Yaping Zong</name><affiliations><json:string>Full Moon Biosystems, 754 N. 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Pastoria Avenue</street><postCode>94085</postCode><settlement>Sunnyvale</settlement><region>CA</region><country key="US">UNITED STATES</country></address></affiliation></author><author><persName><forename type="first">Leming</forename><surname>Shi</surname></persName><affiliation><orgName type="department">National Center for Toxicological Research</orgName><orgName type="institution">US Food and Drug Administration (FDA)</orgName><address><street>3900 NCTR Road</street><postCode>72079</postCode><settlement>Jefferson</settlement><region>AR</region><country key="US">UNITED STATES</country></address></affiliation></author><author role="corresp"><persName><forename type="first">Lili</forename><surname>Wang</surname></persName><email>lili.wang@nist.gov</email><affiliation><orgName type="department">Biochemical Science Division</orgName><orgName type="institution">National Institute of Standards and Technology (NIST)</orgName><address><street>100 Bureau Drive MS 8312</street><postCode>20899-8312</postCode><settlement>Gaithersburg</settlement><region>Maryland</region><country key="US">UNITED STATES</country></address></affiliation></author><idno type="istex">DCAC7330DBF0E7C0C5227FD5A2791AFF6A99B8DB</idno><idno type="ark">ark:/67375/HCB-5W7H6B06-H</idno><idno type="DOI">10.1007/4243_2008_036</idno></analytic><monogr><title level="m" type="main">Standardization and Quality Assurance in Fluorescence Measurements II</title><title level="m" type="sub">Bioanalytical and Biomedical Applications</title><title level="m" type="part">Fluorescence-Based Microarray Technology:
Applications, Future Trends, and Need for Standardization</title><idno type="DOI">10.1007/978-3-540-70571-0</idno><idno type="book-id">978-3-540-70571-0</idno><idno type="ISBN">978-3-540-70570-3</idno><idno type="eISBN">978-3-540-70571-0</idno><idno type="chapter-id">Chap8</idno><idno type="part-id">Part3</idno><editor><persName><forename type="first">Ute</forename><surname>Resch-Genger</surname></persName><email>ute.resch@bam.de</email></editor><imprint><biblScope unit="vol">6</biblScope><biblScope unit="page" from="215">215</biblScope><biblScope unit="page" to="237">237</biblScope><biblScope unit="chapter-count">22</biblScope><biblScope unit="part-chapter-count">4</biblScope></imprint></monogr><series><title level="s" type="main" xml:lang="en">Springer Series on Fluorescence</title><idno type="pISSN">1617-1306</idno><idno type="eISSN">1865-1313</idno><idno type="seriesID">4243</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en"><head>Abstract</head><p>Microarray technology allows high-throughput analysis of tens or even hundreds of thousands of genes
in a single experiment, and has been applied broadly to address a wide variety of questions in
basic and applied sciences. The applications of the microarray technology range from gene-expression profiling
and genotyping to DNA–protein interactions and genome sequencing, and the list of applications keeps
growing, especially when combined with other technologies such as proteomic technologies. However, many
steps are involved in each microarray experiment and a number of microarray platforms exist, each with
its unique features. The challenge is how to standardize the methods and materials to allow intra- and
inter-comparison of microarray data collected in the same or different set of experiments. With the challenge
in mind, we address the need for standardization for each step of the microarray experiments with emphasis
on quality control of array fabrication and scanner calibration and verification. The proposed standards
are designed for checking the quality of mRNA, fabricating slides, hybridization, and collecting, analyzing,
and storing data. By implementing standards for each step of the microarray process, the full potential
of the microarray technology will be realized, especially in the area of disease diagnosis and drug development.
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in a single experiment, and has been applied broadly to address a wide variety of questions in
basic and applied sciences. The applications of the microarray technology range from gene-expression profiling
and genotyping to DNA–protein interactions and genome sequencing, and the list of applications keeps
growing, especially when combined with other technologies such as proteomic technologies. However, many
steps are involved in each microarray experiment and a number of microarray platforms exist, each with
its unique features. The challenge is how to standardize the methods and materials to allow intra- and
inter-comparison of microarray data collected in the same or different set of experiments. With the challenge
in mind, we address the need for standardization for each step of the microarray experiments with emphasis
on quality control of array fabrication and scanner calibration and verification. The proposed standards
are designed for checking the quality of mRNA, fabricating slides, hybridization, and collecting, analyzing,
and storing data. By implementing standards for each step of the microarray process, the full potential
of the microarray technology will be realized, especially in the area of disease diagnosis and drug development.
</Para><Figure Category="Standard" Float="No" ID="Figa"><MediaObject ID="MOESM1"><ImageObject Color="BlackWhite" FileRef="MediaObjects/978-3-540-70571-0_36_Fig1_HTML.gif" Format="GIF" Rendition="HTML" Type="Linedraw"></ImageObject></MediaObject></Figure></Abstract><KeywordGroup Language="En"><Keyword>Bead-based arrays</Keyword><Keyword>cDNA arrays</Keyword><Keyword>Expression profiling</Keyword><Keyword>External RNA control</Keyword><Keyword>Genotyping</Keyword><Keyword>Oligonucleotide arrays</Keyword><Keyword>Scanner performance validation </Keyword></KeywordGroup></ChapterHeader><NoBody></NoBody></Chapter></Part></Book></Series></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>DNA Microarrays: Applications, Future Trends, and the Need for Standardization</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>DNA Microarrays: Applications, Future Trends, and the Need for Standardization</title></titleInfo><name type="personal"><namePart type="given">Sige</namePart><namePart type="family">Zou</namePart><affiliation>Laboratory of Experimental Gerontology, National Institute on Aging, National Institutes of Health (NIH), 5600 Nathan Shock Drive, 21224, Baltimore, MD, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hua-Jun</namePart><namePart type="family">He</namePart><affiliation>Biochemical Science Division, National Institute of Standards and Technology (NIST), 100 Bureau Drive MS 8312, 20899-8312, Gaithersburg, Maryland, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yaping</namePart><namePart type="family">Zong</namePart><affiliation>Full Moon Biosystems, 754 N. Pastoria Avenue, 94085, Sunnyvale, CA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Leming</namePart><namePart type="family">Shi</namePart><affiliation>National Center for Toxicological Research, US Food and Drug Administration (FDA), 3900 NCTR Road, 72079, Jefferson, AR, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Lili</namePart><namePart type="family">Wang</namePart><affiliation>Biochemical Science Division, National Institute of Standards and Technology (NIST), 100 Bureau Drive MS 8312, 20899-8312, Gaithersburg, Maryland, USA</affiliation><affiliation>E-mail: lili.wang@nist.gov</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" type="research-article" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Berlin Heidelberg</publisher><place><placeTerm type="text">Berlin, Heidelberg</placeTerm></place><dateIssued encoding="w3cdtf">2008</dateIssued><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Microarray technology allows high-throughput analysis of tens or even hundreds of thousands of genes in a single experiment, and has been applied broadly to address a wide variety of questions in basic and applied sciences. The applications of the microarray technology range from gene-expression profiling and genotyping to DNA–protein interactions and genome sequencing, and the list of applications keeps growing, especially when combined with other technologies such as proteomic technologies. However, many steps are involved in each microarray experiment and a number of microarray platforms exist, each with its unique features. The challenge is how to standardize the methods and materials to allow intra- and inter-comparison of microarray data collected in the same or different set of experiments. With the challenge in mind, we address the need for standardization for each step of the microarray experiments with emphasis on quality control of array fabrication and scanner calibration and verification. The proposed standards are designed for checking the quality of mRNA, fabricating slides, hybridization, and collecting, analyzing, and storing data. By implementing standards for each step of the microarray process, the full potential of the microarray technology will be realized, especially in the area of disease diagnosis and drug development.</abstract><subject lang="en"><topic>Bead-based arrays</topic><topic>cDNA arrays</topic><topic>Expression profiling</topic><topic>External RNA control</topic><topic>Genotyping</topic><topic>Oligonucleotide arrays</topic><topic>Scanner performance validation</topic></subject><relatedItem type="host"><titleInfo><title>Standardization and Quality Assurance in Fluorescence Measurements II</title><subTitle>Bioanalytical and Biomedical Applications</subTitle></titleInfo><name type="personal"><namePart type="given">Ute</namePart><namePart type="family">Resch-Genger</namePart><role><roleTerm type="text">editor</roleTerm></role></name><genre type="book-series" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0G6R5W5T-Z">book-series</genre><originInfo><publisher>Springer</publisher><copyrightDate encoding="w3cdtf">2008</copyrightDate><issuance>monographic</issuance></originInfo><subject><genre>Book-Subject-Collection</genre><topic authority="SpringerSubjectCodes" authorityURI="SUCO11644">Chemistry and Materials Science</topic></subject><subject><genre>Book-Subject-Group</genre><topic authority="SpringerSubjectCodes" authorityURI="SCC">Chemistry</topic><topic authority="SpringerSubjectCodes" authorityURI="SCC11006">Analytical Chemistry</topic><topic authority="SpringerSubjectCodes" authorityURI="SCB1700X">Molecular Medicine</topic><topic authority="SpringerSubjectCodes" authorityURI="SCH35005">Medical Biochemistry</topic><topic authority="SpringerSubjectCodes" authorityURI="SCP31040">Measurement Science and Instrumentation</topic><topic authority="SpringerSubjectCodes" authorityURI="SCZ17000">Characterization and Evaluation of Materials</topic></subject><identifier type="DOI">10.1007/978-3-540-70571-0</identifier><identifier type="ISBN">978-3-540-70570-3</identifier><identifier type="eISBN">978-3-540-70571-0</identifier><identifier type="ISSN">1617-1306</identifier><identifier type="eISSN">1865-1313</identifier><identifier type="BookTitleID">175955</identifier><identifier type="BookID">978-3-540-70571-0</identifier><identifier type="BookChapterCount">22</identifier><identifier type="BookVolumeNumber">6</identifier><identifier type="BookSequenceNumber">6</identifier><identifier type="PartChapterCount">4</identifier><part><date>2008</date><detail type="part"><title>Part C: Fluorescence-Based Microarray Technology: Applications, Future Trends, and Need for Standardization</title></detail><detail type="volume"><number>6</number><caption>vol.</caption></detail><extent unit="pages"><start>215</start><end>237</end></extent></part><recordInfo><recordOrigin>Springer-Verlag Berlin Heidelberg, 2008</recordOrigin></recordInfo></relatedItem><relatedItem type="series"><titleInfo><title>Springer Series on Fluorescence</title></titleInfo><originInfo><publisher>Springer</publisher><copyrightDate encoding="w3cdtf">2008</copyrightDate><issuance>serial</issuance></originInfo><identifier type="ISSN">1617-1306</identifier><identifier type="eISSN">1865-1313</identifier><identifier type="SeriesID">4243</identifier><part><detail type="volume"><number>Part C</number><caption>vol.</caption></detail></part><recordInfo><recordOrigin>Springer-Verlag Berlin Heidelberg, 2008</recordOrigin></recordInfo></relatedItem><identifier type="istex">DCAC7330DBF0E7C0C5227FD5A2791AFF6A99B8DB</identifier><identifier type="ark">ark:/67375/HCB-5W7H6B06-H</identifier><identifier type="DOI">10.1007/4243_2008_036</identifier><identifier type="ChapterID">36</identifier><identifier type="ChapterID">Chap8</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag Berlin Heidelberg, 2008</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-RLRX46XW-4">springer</recordContentSource><recordOrigin>Springer-Verlag Berlin Heidelberg, 2008</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/HCB-5W7H6B06-H/record.json</uri></json:item></metadata><annexes><json:item><extension>txt</extension><original>true</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/HCB-5W7H6B06-H/annexes.txt</uri></json:item></annexes></istex></record>Pour manipuler ce document sous Unix (Dilib)
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