MersV1, Main, Exploration, bibRecord, 002383

Downregulation of IL-15 and IL-18 in Chicken Embryo Fibroblasts in Response to Virulent or Lentogenic Newcastle Disease Virus Infection

Identifieur interne : 002383 ( Main/Exploration ); précédent : 002382; suivant : 002384

Downregulation of IL-15 and IL-18 in Chicken Embryo Fibroblasts in Response to Virulent or Lentogenic Newcastle Disease Virus Infection

Auteurs : Yuanping Wang [République populaire de Chine] ; Wenquan Liu [République populaire de Chine] ; Mingxing Tian [République populaire de Chine] ; Yan Lin [République populaire de Chine] ; Fangfang Zhao [République populaire de Chine] ; Min Shi [République populaire de Chine] ; Sanjie Cao [République populaire de Chine] ; Xintian Wen [République populaire de Chine] ; Yong Huang [République populaire de Chine]

Source :

Advances in Intelligent and Soft Computing [ 1867-5662 ]

RBID : ISTEX:4D722153E98BFF5C412635185CE245BDEAC2F739

Abstract

Abstract: Newcastle disease virus (NDV) is an important pathogen hazardous to poultry industry, and the pathogenicity of NDV strains varies with different virulence. Chicken Embryo Fibroblasts can serve as a laboratory host systems for NDV, and cytopathic effects (CPE) has some relationship to the strain’s virulence. In order to explore the rhythm of cytokine expression in CEF following velogenic or lentogenic strain infection, NDV virulent strain F48E9 and vaccine strain Lasota were used to infect CEF separately, and cells were collected at 0, 4, 8, 12, 24, 36 hours post-infection (h.p.i.). The total RNA was isolated and real-time quantitative RT-PCR was used to detect the expression of interleukin (IL)-15 and IL-18 gene. The results showed that the expression of both IL-15 and IL-18 gene of F48E9 and Lasota groups were significantly lower than the control group at 4 h, 8 h, 12 h, 24 h.p.i.. But at 36 h.p.i., the expression level of both IL-15 and IL-18 gene of F48E9 group increased rapidly to the level higher than that of control group, while in the Lasota group, the picture was quite different, the expression level of both IL-15 and IL-18 are still lower than that of the control group. The expression change of other cytokines showed no clear regularity in F48E9 or Lasota group. Expression of IL-15 and IL-18 may play an important role in mediation of NDV pathogenesis in CEF.

Url:

https://api.istex.fr/ark:/67375/HCB-M8069HH3-Q/fulltext.pdf

DOI: 10.1007/978-3-642-27537-1_93

Affiliations:

République populaire de Chine

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to stream Main, to step Curation: 002383

Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: Newcastle disease virus (NDV) is an important pathogen hazardous to poultry industry, and the pathogenicity of NDV strains varies with different virulence. Chicken Embryo Fibroblasts can serve as a laboratory host systems for NDV, and cytopathic effects (CPE) has some relationship to the strain’s virulence. In order to explore the rhythm of cytokine expression in CEF following velogenic or lentogenic strain infection, NDV virulent strain F48E9 and vaccine strain Lasota were used to infect CEF separately, and cells were collected at 0, 4, 8, 12, 24, 36 hours post-infection (h.p.i.). The total RNA was isolated and real-time quantitative RT-PCR was used to detect the expression of interleukin (IL)-15 and IL-18 gene. The results showed that the expression of both IL-15 and IL-18 gene of F48E9 and Lasota groups were significantly lower than the control group at 4 h, 8 h, 12 h, 24 h.p.i.. But at 36 h.p.i., the expression level of both IL-15 and IL-18 gene of F48E9 group increased rapidly to the level higher than that of control group, while in the Lasota group, the picture was quite different, the expression level of both IL-15 and IL-18 are still lower than that of the control group. The expression change of other cytokines showed no clear regularity in F48E9 or Lasota group. Expression of IL-15 and IL-18 may play an important role in mediation of NDV pathogenesis in CEF.</div>
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Downregulation of IL-15 and IL-18 in Chicken Embryo Fibroblasts in Response to Virulent or Lentogenic Newcastle Disease Virus Infection

Downregulation of IL-15 and IL-18 in Chicken Embryo Fibroblasts in Response to Virulent or Lentogenic Newcastle Disease Virus Infection

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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