Downregulation of IL-15 and IL-18 in Chicken Embryo Fibroblasts in Response to Virulent or Lentogenic Newcastle Disease Virus Infection
Identifieur interne : 000C05 ( Istex/Corpus ); précédent : 000C04; suivant : 000C06Downregulation of IL-15 and IL-18 in Chicken Embryo Fibroblasts in Response to Virulent or Lentogenic Newcastle Disease Virus Infection
Auteurs : Yuanping Wang ; Wenquan Liu ; Mingxing Tian ; Yan Lin ; Fangfang Zhao ; Min Shi ; Sanjie Cao ; Xintian Wen ; Yong HuangSource :
- Advances in Intelligent and Soft Computing [ 1867-5662 ]
Abstract
Abstract: Newcastle disease virus (NDV) is an important pathogen hazardous to poultry industry, and the pathogenicity of NDV strains varies with different virulence. Chicken Embryo Fibroblasts can serve as a laboratory host systems for NDV, and cytopathic effects (CPE) has some relationship to the strain’s virulence. In order to explore the rhythm of cytokine expression in CEF following velogenic or lentogenic strain infection, NDV virulent strain F48E9 and vaccine strain Lasota were used to infect CEF separately, and cells were collected at 0, 4, 8, 12, 24, 36 hours post-infection (h.p.i.). The total RNA was isolated and real-time quantitative RT-PCR was used to detect the expression of interleukin (IL)-15 and IL-18 gene. The results showed that the expression of both IL-15 and IL-18 gene of F48E9 and Lasota groups were significantly lower than the control group at 4 h, 8 h, 12 h, 24 h.p.i.. But at 36 h.p.i., the expression level of both IL-15 and IL-18 gene of F48E9 group increased rapidly to the level higher than that of control group, while in the Lasota group, the picture was quite different, the expression level of both IL-15 and IL-18 are still lower than that of the control group. The expression change of other cytokines showed no clear regularity in F48E9 or Lasota group. Expression of IL-15 and IL-18 may play an important role in mediation of NDV pathogenesis in CEF.
Url:
DOI: 10.1007/978-3-642-27537-1_93
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sort="Cao, Sanjie" uniqKey="Cao S" first="Sanjie" last="Cao">Sanjie Cao</name><affiliation><mods:affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Wen, Xintian" sort="Wen, Xintian" uniqKey="Wen X" first="Xintian" last="Wen">Xintian Wen</name><affiliation><mods:affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Huang, Yong" sort="Huang, Yong" uniqKey="Huang Y" first="Yong" last="Huang">Yong Huang</name><affiliation><mods:affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: hyong601@163.com</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno 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first="Wenquan" last="Liu">Wenquan Liu</name><affiliation><mods:affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Tian, Mingxing" sort="Tian, Mingxing" uniqKey="Tian M" first="Mingxing" last="Tian">Mingxing Tian</name><affiliation><mods:affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Lin, Yan" sort="Lin, Yan" uniqKey="Lin Y" first="Yan" last="Lin">Yan Lin</name><affiliation><mods:affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Zhao, Fangfang" sort="Zhao, Fangfang" uniqKey="Zhao F" first="Fangfang" last="Zhao">Fangfang Zhao</name><affiliation><mods:affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Shi, Min" sort="Shi, Min" uniqKey="Shi M" first="Min" last="Shi">Min Shi</name><affiliation><mods:affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Cao, Sanjie" sort="Cao, Sanjie" uniqKey="Cao S" first="Sanjie" last="Cao">Sanjie Cao</name><affiliation><mods:affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Wen, Xintian" sort="Wen, Xintian" uniqKey="Wen X" first="Xintian" last="Wen">Xintian Wen</name><affiliation><mods:affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Huang, Yong" sort="Huang, Yong" uniqKey="Huang Y" first="Yong" last="Huang">Yong Huang</name><affiliation><mods:affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: hyong601@163.com</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="s" type="main" xml:lang="en">Advances in Intelligent and Soft Computing</title><title level="s" type="abbrev">Adv.Intel.,Soft Computing</title><idno type="ISSN">1867-5662</idno><idno type="eISSN">1867-5670</idno><idno type="ISSN">1867-5662</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1867-5662</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Newcastle disease virus (NDV) is an important pathogen hazardous to poultry industry, and the pathogenicity of NDV strains varies with different virulence. Chicken Embryo Fibroblasts can serve as a laboratory host systems for NDV, and cytopathic effects (CPE) has some relationship to the strain’s virulence. In order to explore the rhythm of cytokine expression in CEF following velogenic or lentogenic strain infection, NDV virulent strain F48E9 and vaccine strain Lasota were used to infect CEF separately, and cells were collected at 0, 4, 8, 12, 24, 36 hours post-infection (h.p.i.). The total RNA was isolated and real-time quantitative RT-PCR was used to detect the expression of interleukin (IL)-15 and IL-18 gene. The results showed that the expression of both IL-15 and IL-18 gene of F48E9 and Lasota groups were significantly lower than the control group at 4 h, 8 h, 12 h, 24 h.p.i.. But at 36 h.p.i., the expression level of both IL-15 and IL-18 gene of F48E9 group increased rapidly to the level higher than that of control group, while in the Lasota group, the picture was quite different, the expression level of both IL-15 and IL-18 are still lower than that of the control group. The expression change of other cytokines showed no clear regularity in F48E9 or Lasota group. Expression of IL-15 and IL-18 may play an important role in mediation of NDV pathogenesis in CEF.</div></front></TEI><istex><corpusName>springer-ebooks</corpusName><author><json:item><name>Yuanping Wang</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>Wenquan Liu</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>Mingxing Tian</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>Yan Lin</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>Fangfang Zhao</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>Min Shi</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>Sanjie Cao</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>Xintian Wen</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>Yong Huang</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</json:string><json:string>E-mail: hyong601@163.com</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>CEF</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Newcastle disease virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cytokine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>IL-15</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>IL-18</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Real-time PCR</value></json:item></subject><arkIstex>ark:/67375/HCB-M8069HH3-Q</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Newcastle disease virus (NDV) is an important pathogen hazardous to poultry industry, and the pathogenicity of NDV strains varies with different virulence. Chicken Embryo Fibroblasts can serve as a laboratory host systems for NDV, and cytopathic effects (CPE) has some relationship to the strain’s virulence. In order to explore the rhythm of cytokine expression in CEF following velogenic or lentogenic strain infection, NDV virulent strain F48E9 and vaccine strain Lasota were used to infect CEF separately, and cells were collected at 0, 4, 8, 12, 24, 36 hours post-infection (h.p.i.). The total RNA was isolated and real-time quantitative RT-PCR was used to detect the expression of interleukin (IL)-15 and IL-18 gene. The results showed that the expression of both IL-15 and IL-18 gene of F48E9 and Lasota groups were significantly lower than the control group at 4 h, 8 h, 12 h, 24 h.p.i.. But at 36 h.p.i., the expression level of both IL-15 and IL-18 gene of F48E9 group increased rapidly to the level higher than that of control group, while in the Lasota group, the picture was quite different, the expression level of both IL-15 and IL-18 are still lower than that of the control group. The expression change of other cytokines showed no clear regularity in F48E9 or Lasota group. Expression of IL-15 and IL-18 may play an important role in mediation of NDV pathogenesis in CEF.</abstract><qualityIndicators><score>9.291</score><pdfWordCount>4519</pdfWordCount><pdfCharCount>27765</pdfCharCount><pdfVersion>1.6</pdfVersion><pdfPageCount>12</pdfPageCount><pdfPageSize>429.442 x 659.895 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>231</abstractWordCount><abstractCharCount>1399</abstractCharCount><keywordCount>6</keywordCount></qualityIndicators><title>Downregulation of IL-15 and IL-18 in Chicken Embryo Fibroblasts in Response to Virulent or Lentogenic Newcastle Disease Virus Infection</title><chapterId><json:string>93</json:string><json:string>Chap93</json:string></chapterId><genre><json:string>research-article</json:string></genre><serie><title>Advances in Intelligent and Soft Computing</title><language><json:string>unknown</json:string></language><copyrightDate>2012</copyrightDate><issn><json:string>1867-5662</json:string></issn><eissn><json:string>1867-5670</json:string></eissn></serie><host><title>Information Technology and Agricultural Engineering</title><language><json:string>unknown</json:string></language><copyrightDate>2012</copyrightDate><doi><json:string>10.1007/978-3-642-27537-1</json:string></doi><issn><json:string>1867-5662</json:string></issn><eissn><json:string>1867-5670</json:string></eissn><eisbn><json:string>978-3-642-27537-1</json:string></eisbn><bookId><json:string>978-3-642-27537-1</json:string></bookId><isbn><json:string>978-3-642-27536-4</json:string></isbn><volume>134</volume><pages><first>789</first><last>800</last></pages><genre><json:string>book-series</json:string></genre><editor><json:item><name>Egui Zhu</name><affiliations><json:string>South China Normal University, 510631, Guangzhou, China, People's Republic</json:string><json:string>E-mail: scnuzhu@yahoo.ca</json:string></affiliations></json:item><json:item><name>Sabo Sambath</name><affiliations><json:string>South China Normal University, 510631, Guangzhou, China, People's Republic</json:string><json:string>E-mail: sambath@yeah.net</json:string></affiliations></json:item></editor><subject><json:item><value>Engineering</value></json:item><json:item><value>Engineering</value></json:item><json:item><value>Computational Intelligence</value></json:item><json:item><value>Artificial Intelligence (incl. Robotics)</value></json:item><json:item><value>Machinery and Machine Elements</value></json:item></subject></host><ark><json:string>ark:/67375/HCB-M8069HH3-Q</json:string></ark><publicationDate>2012</publicationDate><copyrightDate>2012</copyrightDate><doi><json:string>10.1007/978-3-642-27537-1_93</json:string></doi><id>4D722153E98BFF5C412635185CE245BDEAC2F739</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/HCB-M8069HH3-Q/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/HCB-M8069HH3-Q/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/HCB-M8069HH3-Q/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Downregulation of IL-15 and IL-18 in Chicken Embryo Fibroblasts in Response to Virulent 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type="institution">Sichuan Agricultural University</orgName><address><settlement>Ya’an</settlement><region>Sichuan</region><postCode>625014</postCode><country key="CN">CHINA</country></address></affiliation></author><author><persName><forename type="first">Wenquan</forename><surname>Liu</surname></persName><affiliation><orgName type="department">College of Veterinary Medicine</orgName><orgName type="institution">Sichuan Agricultural University</orgName><address><settlement>Ya’an</settlement><region>Sichuan</region><postCode>625014</postCode><country key="CN">CHINA</country></address></affiliation></author><author><persName><forename type="first">Mingxing</forename><surname>Tian</surname></persName><affiliation><orgName type="department">College of Veterinary Medicine</orgName><orgName type="institution">Sichuan Agricultural University</orgName><address><settlement>Ya’an</settlement><region>Sichuan</region><postCode>625014</postCode><country key="CN">CHINA</country></address></affiliation></author><author><persName><forename type="first">Yan</forename><surname>Lin</surname></persName><affiliation><orgName type="department">College of Veterinary Medicine</orgName><orgName type="institution">Sichuan Agricultural University</orgName><address><settlement>Ya’an</settlement><region>Sichuan</region><postCode>625014</postCode><country key="CN">CHINA</country></address></affiliation></author><author><persName><forename type="first">Fangfang</forename><surname>Zhao</surname></persName><affiliation><orgName type="department">College of Veterinary Medicine</orgName><orgName type="institution">Sichuan Agricultural University</orgName><address><settlement>Ya’an</settlement><region>Sichuan</region><postCode>625014</postCode><country key="CN">CHINA</country></address></affiliation></author><author><persName><forename type="first">Min</forename><surname>Shi</surname></persName><affiliation><orgName type="department">College of Veterinary Medicine</orgName><orgName type="institution">Sichuan Agricultural University</orgName><address><settlement>Ya’an</settlement><region>Sichuan</region><postCode>625014</postCode><country key="CN">CHINA</country></address></affiliation></author><author><persName><forename type="first">Sanjie</forename><surname>Cao</surname></persName><affiliation><orgName type="department">College of Veterinary Medicine</orgName><orgName type="institution">Sichuan Agricultural University</orgName><address><settlement>Ya’an</settlement><region>Sichuan</region><postCode>625014</postCode><country key="CN">CHINA</country></address></affiliation></author><author><persName><forename type="first">Xintian</forename><surname>Wen</surname></persName><affiliation><orgName type="department">College of Veterinary Medicine</orgName><orgName type="institution">Sichuan Agricultural University</orgName><address><settlement>Ya’an</settlement><region>Sichuan</region><postCode>625014</postCode><country key="CN">CHINA</country></address></affiliation></author><author><persName><forename type="first">Yong</forename><surname>Huang</surname></persName><email>hyong601@163.com</email><affiliation><orgName type="department">College of Veterinary Medicine</orgName><orgName type="institution">Sichuan Agricultural University</orgName><address><settlement>Ya’an</settlement><region>Sichuan</region><postCode>625014</postCode><country key="CN">CHINA</country></address></affiliation></author><idno type="istex">4D722153E98BFF5C412635185CE245BDEAC2F739</idno><idno type="ark">ark:/67375/HCB-M8069HH3-Q</idno><idno type="DOI">10.1007/978-3-642-27537-1_93</idno></analytic><monogr><title level="m" type="main">Information Technology and Agricultural Engineering</title><idno type="DOI">10.1007/978-3-642-27537-1</idno><idno type="book-id">978-3-642-27537-1</idno><idno type="ISBN">978-3-642-27536-4</idno><idno type="eISBN">978-3-642-27537-1</idno><idno type="chapter-id">Chap93</idno><editor><persName><forename type="first">Egui</forename><surname>Zhu</surname></persName><email>scnuzhu@yahoo.ca</email><affiliation><orgName type="institution">South China Normal University</orgName><address><settlement>Guangzhou</settlement><postCode>510631</postCode><country key=""></country></address></affiliation></editor><editor><persName><forename type="first">Sabo</forename><surname>Sambath</surname></persName><email>sambath@yeah.net</email><affiliation><orgName type="institution">South China Normal University</orgName><address><settlement>Guangzhou</settlement><postCode>510631</postCode><country key=""></country></address></affiliation></editor><imprint><biblScope unit="vol">134</biblScope><biblScope unit="page" from="789">789</biblScope><biblScope unit="page" to="800">800</biblScope><biblScope unit="chapter-count">117</biblScope></imprint></monogr><series><title level="s" type="main" xml:lang="en">Advances in Intelligent and Soft Computing</title><title level="s" type="abbrev">Adv.Intel.,Soft Computing</title><idno type="pISSN">1867-5662</idno><idno type="eISSN">1867-5670</idno><idno type="seriesID">4240</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en"><head>Abstract</head><p>Newcastle disease virus (NDV) is an important pathogen hazardous to poultry industry, and the pathogenicity of NDV strains varies with different virulence. Chicken Embryo Fibroblasts can serve as a laboratory host systems for NDV, and cytopathic effects (CPE) has some relationship to the strain’s virulence. In order to explore the rhythm of cytokine expression in CEF following velogenic or lentogenic strain infection, NDV virulent strain F48E9 and vaccine strain Lasota were used to infect CEF separately, and cells were collected at 0, 4, 8, 12, 24, 36 hours post-infection (h.p.i.). The total RNA was isolated and real-time quantitative RT-PCR was used to detect the expression of interleukin (IL)-15 and IL-18 gene. The results showed that the expression of both IL-15 and IL-18 gene of F48E9 and Lasota groups were significantly lower than the control group at 4 h, 8 h, 12 h, 24 h.p.i.. But at 36 h.p.i., the expression level of both IL-15 and IL-18 gene of F48E9 group increased rapidly to the level higher than that of control group, while in the Lasota group, the picture was quite different, the expression level of both IL-15 and IL-18 are still lower than that of the control group. The expression change of other cytokines showed no clear regularity in F48E9 or Lasota group. Expression of IL-15 and IL-18 may play an important role in mediation of NDV pathogenesis in CEF.</p></abstract><textClass ana="keyword"><keywords xml:lang="en"><term>CEF</term><term>Newcastle disease virus</term><term>cytokine</term><term>IL-15</term><term>IL-18</term><term>Real-time PCR</term></keywords></textClass><textClass ana="subject"><keywords scheme="book-subject-collection"><list><label>SUCO11647</label><item><term>Engineering</term></item></list></keywords></textClass><textClass ana="subject"><keywords scheme="book-subject"><list><label>SCT</label><item><term type="Primary">Engineering</term></item><label>SCT11014</label><item><term type="Secondary" subtype="priority-1">Computational Intelligence</term></item><label>SCI21017</label><item><term type="Secondary" subtype="priority-2">Artificial Intelligence (incl. Robotics)</term></item><label>SCT17039</label><item><term type="Secondary" subtype="priority-3">Machinery and Machine Elements</term></item></list></keywords></textClass><langUsage><language ident="EN"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/HCB-M8069HH3-Q/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-ebooks not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer Berlin Heidelberg</PublisherName><PublisherLocation>Berlin, Heidelberg</PublisherLocation></PublisherInfo><Series><SeriesInfo SeriesType="Series" TocLevels="0"><SeriesID>4240</SeriesID><SeriesPrintISSN>1867-5662</SeriesPrintISSN><SeriesElectronicISSN>1867-5670</SeriesElectronicISSN><SeriesTitle Language="En">Advances in Intelligent and Soft Computing</SeriesTitle><SeriesAbbreviatedTitle>Adv.Intel.,Soft Computing</SeriesAbbreviatedTitle></SeriesInfo><Book Language="En" OutputMedium="All"><BookInfo BookProductType="Monograph" ContainsESM="No" Language="En" MediaType="eBook" NumberingDepth="2" NumberingStyle="ContentOnly" OutputMedium="All" TocLevels="0"><BookID>978-3-642-27537-1</BookID><BookTitle>Information Technology and Agricultural Engineering</BookTitle><BookVolumeNumber>134</BookVolumeNumber><BookSequenceNumber>134</BookSequenceNumber><BookDOI>10.1007/978-3-642-27537-1</BookDOI><BookTitleID>272260</BookTitleID><BookPrintISBN>978-3-642-27536-4</BookPrintISBN><BookElectronicISBN>978-3-642-27537-1</BookElectronicISBN><BookEdition>2012</BookEdition><BookChapterCount>117</BookChapterCount><BookCopyright><CopyrightHolderName>Springer-Verlag GmbH Berlin Heidelberg</CopyrightHolderName><CopyrightYear>2012</CopyrightYear></BookCopyright><BookSubjectGroup><BookSubject Code="SCT" Type="Primary">Engineering</BookSubject><BookSubject Code="SCT11014" Priority="1" Type="Secondary">Computational Intelligence</BookSubject><BookSubject Code="SCI21017" Priority="2" Type="Secondary">Artificial Intelligence (incl. Robotics)</BookSubject><BookSubject Code="SCT17039" Priority="3" Type="Secondary">Machinery and Machine Elements</BookSubject><SubjectCollection Code="SUCO11647">Engineering</SubjectCollection></BookSubjectGroup><BookContext><SeriesID>4240</SeriesID></BookContext></BookInfo><BookHeader><EditorGroup><Editor AffiliationIDS="AffID1"><EditorName DisplayOrder="Western"><GivenName>Egui</GivenName><FamilyName>Zhu</FamilyName></EditorName><Contact><Email>scnuzhu@yahoo.ca</Email></Contact></Editor><Editor AffiliationIDS="AffID2"><EditorName DisplayOrder="Western"><GivenName>Sabo</GivenName><FamilyName>Sambath</FamilyName></EditorName><Contact><Email>sambath@yeah.net</Email></Contact></Editor><Affiliation ID="AffID1"><OrgName>South China Normal University</OrgName><OrgAddress><City>Guangzhou</City><Postcode>510631</Postcode><Country>China, People's Republic</Country></OrgAddress></Affiliation><Affiliation ID="AffID2"><OrgName>South China Normal University</OrgName><OrgAddress><City>Guangzhou</City><Postcode>510631</Postcode><Country>China, People's Republic</Country></OrgAddress></Affiliation></EditorGroup></BookHeader><Chapter ID="Chap93" Language="En"><ChapterInfo ChapterType="OriginalPaper" ContainsESM="No" NumberingDepth="2" NumberingStyle="ContentOnly" OutputMedium="All" TocLevels="0"><ChapterID>93</ChapterID><ChapterDOI>10.1007/978-3-642-27537-1_93</ChapterDOI><ChapterSequenceNumber>93</ChapterSequenceNumber><ChapterTitle Language="En">Downregulation of IL-15 and IL-18 in Chicken Embryo Fibroblasts in Response to Virulent or Lentogenic Newcastle Disease Virus Infection</ChapterTitle><ChapterFirstPage>789</ChapterFirstPage><ChapterLastPage>800</ChapterLastPage><ChapterCopyright><CopyrightHolderName>Springer-Verlag GmbH Berlin Heidelberg</CopyrightHolderName><CopyrightYear>2012</CopyrightYear></ChapterCopyright><ChapterContext><SeriesID>4240</SeriesID><BookID>978-3-642-27537-1</BookID><BookTitle>Information Technology and Agricultural Engineering</BookTitle></ChapterContext></ChapterInfo><ChapterHeader><AuthorGroup><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yuanping</GivenName><FamilyName>Wang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Wenquan</GivenName><FamilyName>Liu</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Mingxing</GivenName><FamilyName>Tian</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yan</GivenName><FamilyName>Lin</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Fangfang</GivenName><FamilyName>Zhao</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Min</GivenName><FamilyName>Shi</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Sanjie</GivenName><FamilyName>Cao</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Xintian</GivenName><FamilyName>Wen</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yong</GivenName><FamilyName>Huang</FamilyName></AuthorName><Contact><Email>hyong601@163.com</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>College of Veterinary Medicine</OrgDivision><OrgName>Sichuan Agricultural University</OrgName><OrgAddress><City>Ya’an</City><State>Sichuan</State><Postcode>625014</Postcode><Country>China</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Newcastle disease virus (NDV) is an important pathogen hazardous to poultry industry, and the pathogenicity of NDV strains varies with different virulence. Chicken Embryo Fibroblasts can serve as a laboratory host systems for NDV, and cytopathic effects (CPE) has some relationship to the strain’s virulence. In order to explore the rhythm of cytokine expression in CEF following velogenic or lentogenic strain infection, NDV virulent strain F48E9 and vaccine strain Lasota were used to infect CEF separately, and cells were collected at 0, 4, 8, 12, 24, 36 hours post-infection (h.p.i.). The total RNA was isolated and real-time quantitative RT-PCR was used to detect the expression of interleukin (IL)-15 and IL-18 gene. The results showed that the expression of both IL-15 and IL-18 gene of F48E9 and Lasota groups were significantly lower than the control group at 4 h, 8 h, 12 h, 24 h.p.i.. But at 36 h.p.i., the expression level of both IL-15 and IL-18 gene of F48E9 group increased rapidly to the level higher than that of control group, while in the Lasota group, the picture was quite different, the expression level of both IL-15 and IL-18 are still lower than that of the control group. The expression change of other cytokines showed no clear regularity in F48E9 or Lasota group. Expression of IL-15 and IL-18 may play an important role in mediation of NDV pathogenesis in CEF.</Para></Abstract><KeywordGroup Language="En"><Heading>Keywords</Heading><Keyword>CEF</Keyword><Keyword>Newcastle disease virus</Keyword><Keyword>cytokine</Keyword><Keyword>IL-15</Keyword><Keyword>IL-18</Keyword><Keyword>Real-time PCR</Keyword></KeywordGroup></ChapterHeader><NoBody></NoBody></Chapter></Book></Series></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Downregulation of IL-15 and IL-18 in Chicken Embryo Fibroblasts in Response to Virulent or Lentogenic Newcastle Disease Virus Infection</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Downregulation of IL-15 and IL-18 in Chicken Embryo Fibroblasts in Response to Virulent or Lentogenic Newcastle Disease Virus Infection</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Yuanping</namePart><namePart type="family">Wang</namePart><affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wenquan</namePart><namePart type="family">Liu</namePart><affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mingxing</namePart><namePart type="family">Tian</namePart><affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yan</namePart><namePart type="family">Lin</namePart><affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Fangfang</namePart><namePart type="family">Zhao</namePart><affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Min</namePart><namePart type="family">Shi</namePart><affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sanjie</namePart><namePart type="family">Cao</namePart><affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Xintian</namePart><namePart type="family">Wen</namePart><affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yong</namePart><namePart type="family">Huang</namePart><affiliation>College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China</affiliation><affiliation>E-mail: hyong601@163.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" type="research-article" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Berlin Heidelberg</publisher><place><placeTerm type="text">Berlin, Heidelberg</placeTerm></place><dateIssued encoding="w3cdtf">2012</dateIssued><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Newcastle disease virus (NDV) is an important pathogen hazardous to poultry industry, and the pathogenicity of NDV strains varies with different virulence. Chicken Embryo Fibroblasts can serve as a laboratory host systems for NDV, and cytopathic effects (CPE) has some relationship to the strain’s virulence. In order to explore the rhythm of cytokine expression in CEF following velogenic or lentogenic strain infection, NDV virulent strain F48E9 and vaccine strain Lasota were used to infect CEF separately, and cells were collected at 0, 4, 8, 12, 24, 36 hours post-infection (h.p.i.). The total RNA was isolated and real-time quantitative RT-PCR was used to detect the expression of interleukin (IL)-15 and IL-18 gene. The results showed that the expression of both IL-15 and IL-18 gene of F48E9 and Lasota groups were significantly lower than the control group at 4 h, 8 h, 12 h, 24 h.p.i.. But at 36 h.p.i., the expression level of both IL-15 and IL-18 gene of F48E9 group increased rapidly to the level higher than that of control group, while in the Lasota group, the picture was quite different, the expression level of both IL-15 and IL-18 are still lower than that of the control group. The expression change of other cytokines showed no clear regularity in F48E9 or Lasota group. Expression of IL-15 and IL-18 may play an important role in mediation of NDV pathogenesis in CEF.</abstract><subject lang="en"><genre>Keywords</genre><topic>CEF</topic><topic>Newcastle disease virus</topic><topic>cytokine</topic><topic>IL-15</topic><topic>IL-18</topic><topic>Real-time PCR</topic></subject><relatedItem type="host"><titleInfo><title>Information Technology and Agricultural Engineering</title></titleInfo><name type="personal"><namePart type="given">Egui</namePart><namePart type="family">Zhu</namePart><affiliation>South China Normal University, 510631, Guangzhou, China, People's Republic</affiliation><affiliation>E-mail: scnuzhu@yahoo.ca</affiliation><role><roleTerm type="text">editor</roleTerm></role></name><name type="personal"><namePart type="given">Sabo</namePart><namePart type="family">Sambath</namePart><affiliation>South China Normal University, 510631, Guangzhou, China, People's Republic</affiliation><affiliation>E-mail: sambath@yeah.net</affiliation><role><roleTerm type="text">editor</roleTerm></role></name><genre type="book-series" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0G6R5W5T-Z">book-series</genre><originInfo><publisher>Springer</publisher><copyrightDate encoding="w3cdtf">2012</copyrightDate><issuance>monographic</issuance><edition>2012</edition></originInfo><subject><genre>Book-Subject-Collection</genre><topic authority="SpringerSubjectCodes" authorityURI="SUCO11647">Engineering</topic></subject><subject><genre>Book-Subject-Group</genre><topic authority="SpringerSubjectCodes" authorityURI="SCT">Engineering</topic><topic authority="SpringerSubjectCodes" authorityURI="SCT11014">Computational Intelligence</topic><topic authority="SpringerSubjectCodes" authorityURI="SCI21017">Artificial Intelligence (incl. 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