Inactivated RNA polymerase II open complexes can be reactivated with TFIIE.
Identifieur interne : 002248 ( Main/Exploration ); précédent : 002247; suivant : 002249Inactivated RNA polymerase II open complexes can be reactivated with TFIIE.
Auteurs : Pavel Abart [États-Unis] ; Donal S. LuseSource :
- The Journal of biological chemistry [ 1083-351X ] ; 2012.
Descripteurs français
- KwdFr :
- Adénosine triphosphate (), Adénosine triphosphate (génétique), Adénosine triphosphate (métabolisme), Cellules HeLa, Facteur de transcription TFIIH (), Facteur de transcription TFIIH (génétique), Facteur de transcription TFIIH (métabolisme), Facteurs de transcription TFII (), Facteurs de transcription TFII (génétique), Facteurs de transcription TFII (métabolisme), Humains, RNA polymerase II (), RNA polymerase II (génétique), RNA polymerase II (métabolisme), Transcription génétique (physiologie).
- MESH :
- génétique : Adénosine triphosphate, Facteur de transcription TFIIH, Facteurs de transcription TFII, RNA polymerase II.
- métabolisme : Adénosine triphosphate, Facteur de transcription TFIIH, Facteurs de transcription TFII, RNA polymerase II.
- physiologie : Transcription génétique.
- Adénosine triphosphate, Cellules HeLa, Facteur de transcription TFIIH, Facteurs de transcription TFII, Humains, RNA polymerase II.
English descriptors
- KwdEn :
- Adenosine Triphosphate (chemistry), Adenosine Triphosphate (genetics), Adenosine Triphosphate (metabolism), HeLa Cells, Humans, RNA Polymerase II (chemistry), RNA Polymerase II (genetics), RNA Polymerase II (metabolism), Transcription Factor TFIIH (chemistry), Transcription Factor TFIIH (genetics), Transcription Factor TFIIH (metabolism), Transcription Factors, TFII (chemistry), Transcription Factors, TFII (genetics), Transcription Factors, TFII (metabolism), Transcription, Genetic (physiology).
- MESH :
- chemical , chemistry : Adenosine Triphosphate, RNA Polymerase II, Transcription Factor TFIIH, Transcription Factors, TFII.
- chemical , genetics : Adenosine Triphosphate, RNA Polymerase II, Transcription Factor TFIIH, Transcription Factors, TFII.
- chemical , metabolism : Adenosine Triphosphate, RNA Polymerase II, Transcription Factor TFIIH, Transcription Factors, TFII.
- physiology : Transcription, Genetic.
- HeLa Cells, Humans.
Abstract
Transcript initiation by RNA polymerase II (pol II) requires a helicase within TFIIH to generate the unpaired template strand. However, pol II preinitiation complexes (PICs) lose the ability to synthesize RNA very rapidly upon exposure to ATP alone in the absence of other NTPs. This inactivation is not caused by the TFIIH kinase activity, the loss of transcription factors or pol II from the PIC, or the collapse of the initially formed transcription bubble. TFIIE is necessary for PIC formation, but TFIIE is not retained as a stable component in PICs prepared by our protocol. Nevertheless, activity can be at least partially restored to ATP-treated PICs by the readdition of TFIIE. PICs formed on premelted (bubble) templates require TFIIH for effective transcript elongation to +20. Incubation of bubble template PICs with ATP caused reduced yields of 20-mers, but this effect was partially reversed by the addition of TFIIE. Our results suggest that once the open complex is formed, TFIIH decays into an inactive configuration in the absence of nucleotides for transcription. Although TFIIE does not play a role in transcript initiation itself, inactivation resulting from ATP preincubation can be reversed by a remodeling process mediated by TFIIE. Finally, we have also uncovered a major role for TFIIF in the earliest stages of transcript elongation that is unique to bubble templates.
DOI: 10.1074/jbc.M111.297572
PubMed: 22119917
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001E33
- to stream PubMed, to step Curation: 001E33
- to stream PubMed, to step Checkpoint: 001C74
- to stream Ncbi, to step Merge: 000903
- to stream Ncbi, to step Curation: 000903
- to stream Ncbi, to step Checkpoint: 000903
- to stream Main, to step Merge: 002273
- to stream Main, to step Curation: 002248
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inactivated RNA polymerase II open complexes can be reactivated with TFIIE.</title>
<author><name sortKey=" Abart, Pavel" sort=" Abart, Pavel" uniqKey=" Abart P" first="Pavel" last=" Abart">Pavel Abart</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Genetics, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Molecular Genetics, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195</wicri:regionArea>
<wicri:noRegion>Ohio 44195</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Luse, Donal S" sort="Luse, Donal S" uniqKey="Luse D" first="Donal S" last="Luse">Donal S. Luse</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2012">2012</date>
<idno type="RBID">pubmed:22119917</idno>
<idno type="pmid">22119917</idno>
<idno type="doi">10.1074/jbc.M111.297572</idno>
<idno type="wicri:Area/PubMed/Corpus">001E33</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001E33</idno>
<idno type="wicri:Area/PubMed/Curation">001E33</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001E33</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001C74</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001C74</idno>
<idno type="wicri:Area/Ncbi/Merge">000903</idno>
<idno type="wicri:Area/Ncbi/Curation">000903</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000903</idno>
<idno type="wicri:Area/Main/Merge">002273</idno>
<idno type="wicri:Area/Main/Curation">002248</idno>
<idno type="wicri:Area/Main/Exploration">002248</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Inactivated RNA polymerase II open complexes can be reactivated with TFIIE.</title>
<author><name sortKey=" Abart, Pavel" sort=" Abart, Pavel" uniqKey=" Abart P" first="Pavel" last=" Abart">Pavel Abart</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Genetics, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Molecular Genetics, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195</wicri:regionArea>
<wicri:noRegion>Ohio 44195</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Luse, Donal S" sort="Luse, Donal S" uniqKey="Luse D" first="Donal S" last="Luse">Donal S. Luse</name>
</author>
</analytic>
<series><title level="j">The Journal of biological chemistry</title>
<idno type="eISSN">1083-351X</idno>
<imprint><date when="2012" type="published">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine Triphosphate (chemistry)</term>
<term>Adenosine Triphosphate (genetics)</term>
<term>Adenosine Triphosphate (metabolism)</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>RNA Polymerase II (chemistry)</term>
<term>RNA Polymerase II (genetics)</term>
<term>RNA Polymerase II (metabolism)</term>
<term>Transcription Factor TFIIH (chemistry)</term>
<term>Transcription Factor TFIIH (genetics)</term>
<term>Transcription Factor TFIIH (metabolism)</term>
<term>Transcription Factors, TFII (chemistry)</term>
<term>Transcription Factors, TFII (genetics)</term>
<term>Transcription Factors, TFII (metabolism)</term>
<term>Transcription, Genetic (physiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adénosine triphosphate ()</term>
<term>Adénosine triphosphate (génétique)</term>
<term>Adénosine triphosphate (métabolisme)</term>
<term>Cellules HeLa</term>
<term>Facteur de transcription TFIIH ()</term>
<term>Facteur de transcription TFIIH (génétique)</term>
<term>Facteur de transcription TFIIH (métabolisme)</term>
<term>Facteurs de transcription TFII ()</term>
<term>Facteurs de transcription TFII (génétique)</term>
<term>Facteurs de transcription TFII (métabolisme)</term>
<term>Humains</term>
<term>RNA polymerase II ()</term>
<term>RNA polymerase II (génétique)</term>
<term>RNA polymerase II (métabolisme)</term>
<term>Transcription génétique (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Adenosine Triphosphate</term>
<term>RNA Polymerase II</term>
<term>Transcription Factor TFIIH</term>
<term>Transcription Factors, TFII</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Adenosine Triphosphate</term>
<term>RNA Polymerase II</term>
<term>Transcription Factor TFIIH</term>
<term>Transcription Factors, TFII</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adenosine Triphosphate</term>
<term>RNA Polymerase II</term>
<term>Transcription Factor TFIIH</term>
<term>Transcription Factors, TFII</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adénosine triphosphate</term>
<term>Facteur de transcription TFIIH</term>
<term>Facteurs de transcription TFII</term>
<term>RNA polymerase II</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Adénosine triphosphate</term>
<term>Facteur de transcription TFIIH</term>
<term>Facteurs de transcription TFII</term>
<term>RNA polymerase II</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Transcription génétique</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Transcription, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>HeLa Cells</term>
<term>Humans</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Adénosine triphosphate</term>
<term>Cellules HeLa</term>
<term>Facteur de transcription TFIIH</term>
<term>Facteurs de transcription TFII</term>
<term>Humains</term>
<term>RNA polymerase II</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Transcript initiation by RNA polymerase II (pol II) requires a helicase within TFIIH to generate the unpaired template strand. However, pol II preinitiation complexes (PICs) lose the ability to synthesize RNA very rapidly upon exposure to ATP alone in the absence of other NTPs. This inactivation is not caused by the TFIIH kinase activity, the loss of transcription factors or pol II from the PIC, or the collapse of the initially formed transcription bubble. TFIIE is necessary for PIC formation, but TFIIE is not retained as a stable component in PICs prepared by our protocol. Nevertheless, activity can be at least partially restored to ATP-treated PICs by the readdition of TFIIE. PICs formed on premelted (bubble) templates require TFIIH for effective transcript elongation to +20. Incubation of bubble template PICs with ATP caused reduced yields of 20-mers, but this effect was partially reversed by the addition of TFIIE. Our results suggest that once the open complex is formed, TFIIH decays into an inactive configuration in the absence of nucleotides for transcription. Although TFIIE does not play a role in transcript initiation itself, inactivation resulting from ATP preincubation can be reversed by a remodeling process mediated by TFIIE. Finally, we have also uncovered a major role for TFIIF in the earliest stages of transcript elongation that is unique to bubble templates.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><noCountry><name sortKey="Luse, Donal S" sort="Luse, Donal S" uniqKey="Luse D" first="Donal S" last="Luse">Donal S. Luse</name>
</noCountry>
<country name="États-Unis"><noRegion><name sortKey=" Abart, Pavel" sort=" Abart, Pavel" uniqKey=" Abart P" first="Pavel" last=" Abart">Pavel Abart</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002248 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002248 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:22119917 |texte= Inactivated RNA polymerase II open complexes can be reactivated with TFIIE. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22119917" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |