MersV1, Main, Exploration, bibRecord, 001645

Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia

Identifieur interne : 001645 ( Main/Exploration ); précédent : 001644; suivant : 001646

Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia

Auteurs : Nor-Aziyah Mat-Rahim ; Tengku Rogayah Tengku Abdul Rashid ; Jeyanthi Suppiah ; Ravindran Thayan ; Apandi Mohd Yusof ; Zainah Sa'At

Source :

Asian Pacific Journal of Tropical Disease [ 2222-1808 ] ; 2015.

RBID : PMC:7129196

Abstract

Objective

To describe the complete nucleocapsid (N) gene region of Middle East respiratory syndrome coronavirus (MERS-CoV) from imported case in Malaysia and the relations with human- and camel-derived MERS-CoV.

Methods

Combination of throat and nasal swab specimens was subjected to viral RNA extraction. For screening, the extracted RNA was subjected to real-time RT-PCR targeting upstream of E gene, open reading frame 1b and open reading frame 1a. For confirmation, the RNA was subjected to RT-PCR targeting partial part of the RNA-dependent RNA polymerase and nucleocapsid, followed by amplification of complete N gene region. Nucleotide sequencing of the first Malaysian case of MERS-CoV was performed following the confirmation with real-time RT-PCR detection.

Results

Initial analysis of partial RNA-dependent RNA polymerase and N gene revealed that the nucleotides had high similarity to Jeddah_1_2013 strain. Analysis of complete N gene region (1 242 nucleotides) from the case showed high similarity and yet distinct to the nucleotide sequences of camel-derived MERS-CoV.

Conclusions

From the finding, there are possibilities that the patient acquired the infection from zoonotic transmission from dromedary camels.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129196

DOI: 10.1016/S2222-1808(15)60833-7
PubMed: 32289031
PubMed Central: 7129196

Affiliations:

Le document en format XML

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<author><name sortKey="Mat Rahim, Nor Aziyah" sort="Mat Rahim, Nor Aziyah" uniqKey="Mat Rahim N" first="Nor-Aziyah" last="Mat-Rahim">Nor-Aziyah Mat-Rahim</name>
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<author><name sortKey="Rashid, Tengku Rogayah Tengku Abdul" sort="Rashid, Tengku Rogayah Tengku Abdul" uniqKey="Rashid T" first="Tengku Rogayah Tengku Abdul" last="Rashid">Tengku Rogayah Tengku Abdul Rashid</name>
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<author><name sortKey="Suppiah, Jeyanthi" sort="Suppiah, Jeyanthi" uniqKey="Suppiah J" first="Jeyanthi" last="Suppiah">Jeyanthi Suppiah</name>
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<author><name sortKey="Thayan, Ravindran" sort="Thayan, Ravindran" uniqKey="Thayan R" first="Ravindran" last="Thayan">Ravindran Thayan</name>
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<author><name sortKey="Thayan, Ravindran" sort="Thayan, Ravindran" uniqKey="Thayan R" first="Ravindran" last="Thayan">Ravindran Thayan</name>
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<author><name sortKey="Yusof, Apandi Mohd" sort="Yusof, Apandi Mohd" uniqKey="Yusof A" first="Apandi Mohd" last="Yusof">Apandi Mohd Yusof</name>
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<front><div type="abstract" xml:lang="en"><sec><title>Objective</title>
<p>To describe the complete nucleocapsid (<italic>N</italic>
) gene region of Middle East respiratory syndrome coronavirus (MERS-CoV) from imported case in Malaysia and the relations with human- and camel-derived MERS-CoV.</p>
</sec>
<sec><title>Methods</title>
<p>Combination of throat and nasal swab specimens was subjected to viral RNA extraction. For screening, the extracted RNA was subjected to real-time RT-PCR targeting upstream of <italic>E</italic>
 gene, open reading frame 1b and open reading frame 1a. For confirmation, the RNA was subjected to RT-PCR targeting partial part of the RNA-dependent RNA polymerase and nucleocapsid, followed by amplification of complete <italic>N</italic>
 gene region. Nucleotide sequencing of the first Malaysian case of MERS-CoV was performed following the confirmation with real-time RT-PCR detection.</p>
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<sec><title>Results</title>
<p>Initial analysis of partial RNA-dependent RNA polymerase and <italic>N</italic>
 gene revealed that the nucleotides had high similarity to Jeddah_1_2013 strain. Analysis of complete <italic>N</italic>
 gene region (1 242 nucleotides) from the case showed high similarity and yet distinct to the nucleotide sequences of camel-derived MERS-CoV.</p>
</sec>
<sec><title>Conclusions</title>
<p>From the finding, there are possibilities that the patient acquired the infection from zoonotic transmission from dromedary camels.</p>
</sec>
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Serveur d'exploration MERS

Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia

Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia

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