Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia
Identifieur interne : 001645 ( Main/Exploration ); précédent : 001644; suivant : 001646Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia
Auteurs : Nor-Aziyah Mat-Rahim ; Tengku Rogayah Tengku Abdul Rashid ; Jeyanthi Suppiah ; Ravindran Thayan ; Apandi Mohd Yusof ; Zainah Sa'AtSource :
- Asian Pacific Journal of Tropical Disease [ 2222-1808 ] ; 2015.
Abstract
To describe the complete nucleocapsid (
Combination of throat and nasal swab specimens was subjected to viral RNA extraction. For screening, the extracted RNA was subjected to real-time RT-PCR targeting upstream of
Initial analysis of partial RNA-dependent RNA polymerase and
From the finding, there are possibilities that the patient acquired the infection from zoonotic transmission from dromedary camels.
Url:
DOI: 10.1016/S2222-1808(15)60833-7
PubMed: 32289031
PubMed Central: 7129196
Affiliations:
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<author><name sortKey="Mat Rahim, Nor Aziyah" sort="Mat Rahim, Nor Aziyah" uniqKey="Mat Rahim N" first="Nor-Aziyah" last="Mat-Rahim">Nor-Aziyah Mat-Rahim</name>
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<author><name sortKey="Rashid, Tengku Rogayah Tengku Abdul" sort="Rashid, Tengku Rogayah Tengku Abdul" uniqKey="Rashid T" first="Tengku Rogayah Tengku Abdul" last="Rashid">Tengku Rogayah Tengku Abdul Rashid</name>
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<author><name sortKey="Suppiah, Jeyanthi" sort="Suppiah, Jeyanthi" uniqKey="Suppiah J" first="Jeyanthi" last="Suppiah">Jeyanthi Suppiah</name>
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<author><name sortKey="Thayan, Ravindran" sort="Thayan, Ravindran" uniqKey="Thayan R" first="Ravindran" last="Thayan">Ravindran Thayan</name>
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<author><name sortKey="Yusof, Apandi Mohd" sort="Yusof, Apandi Mohd" uniqKey="Yusof A" first="Apandi Mohd" last="Yusof">Apandi Mohd Yusof</name>
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<author><name sortKey="Sa At, Zainah" sort="Sa At, Zainah" uniqKey="Sa At Z" first="Zainah" last="Sa'At">Zainah Sa'At</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia</title>
<author><name sortKey="Mat Rahim, Nor Aziyah" sort="Mat Rahim, Nor Aziyah" uniqKey="Mat Rahim N" first="Nor-Aziyah" last="Mat-Rahim">Nor-Aziyah Mat-Rahim</name>
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<author><name sortKey="Rashid, Tengku Rogayah Tengku Abdul" sort="Rashid, Tengku Rogayah Tengku Abdul" uniqKey="Rashid T" first="Tengku Rogayah Tengku Abdul" last="Rashid">Tengku Rogayah Tengku Abdul Rashid</name>
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<author><name sortKey="Suppiah, Jeyanthi" sort="Suppiah, Jeyanthi" uniqKey="Suppiah J" first="Jeyanthi" last="Suppiah">Jeyanthi Suppiah</name>
</author>
<author><name sortKey="Thayan, Ravindran" sort="Thayan, Ravindran" uniqKey="Thayan R" first="Ravindran" last="Thayan">Ravindran Thayan</name>
</author>
<author><name sortKey="Yusof, Apandi Mohd" sort="Yusof, Apandi Mohd" uniqKey="Yusof A" first="Apandi Mohd" last="Yusof">Apandi Mohd Yusof</name>
</author>
<author><name sortKey="Sa At, Zainah" sort="Sa At, Zainah" uniqKey="Sa At Z" first="Zainah" last="Sa'At">Zainah Sa'At</name>
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<series><title level="j">Asian Pacific Journal of Tropical Disease</title>
<idno type="eISSN">2222-1808</idno>
<imprint><date when="2015">2015</date>
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<front><div type="abstract" xml:lang="en"><sec><title>Objective</title>
<p>To describe the complete nucleocapsid (<italic>N</italic>
) gene region of Middle East respiratory syndrome coronavirus (MERS-CoV) from imported case in Malaysia and the relations with human- and camel-derived MERS-CoV.</p>
</sec>
<sec><title>Methods</title>
<p>Combination of throat and nasal swab specimens was subjected to viral RNA extraction. For screening, the extracted RNA was subjected to real-time RT-PCR targeting upstream of <italic>E</italic>
gene, open reading frame 1b and open reading frame 1a. For confirmation, the RNA was subjected to RT-PCR targeting partial part of the RNA-dependent RNA polymerase and nucleocapsid, followed by amplification of complete <italic>N</italic>
gene region. Nucleotide sequencing of the first Malaysian case of MERS-CoV was performed following the confirmation with real-time RT-PCR detection.</p>
</sec>
<sec><title>Results</title>
<p>Initial analysis of partial RNA-dependent RNA polymerase and <italic>N</italic>
gene revealed that the nucleotides had high similarity to Jeddah_1_2013 strain. Analysis of complete <italic>N</italic>
gene region (1 242 nucleotides) from the case showed high similarity and yet distinct to the nucleotide sequences of camel-derived MERS-CoV.</p>
</sec>
<sec><title>Conclusions</title>
<p>From the finding, there are possibilities that the patient acquired the infection from zoonotic transmission from dromedary camels.</p>
</sec>
</div>
</front>
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<tree><noCountry><name sortKey="Mat Rahim, Nor Aziyah" sort="Mat Rahim, Nor Aziyah" uniqKey="Mat Rahim N" first="Nor-Aziyah" last="Mat-Rahim">Nor-Aziyah Mat-Rahim</name>
<name sortKey="Rashid, Tengku Rogayah Tengku Abdul" sort="Rashid, Tengku Rogayah Tengku Abdul" uniqKey="Rashid T" first="Tengku Rogayah Tengku Abdul" last="Rashid">Tengku Rogayah Tengku Abdul Rashid</name>
<name sortKey="Sa At, Zainah" sort="Sa At, Zainah" uniqKey="Sa At Z" first="Zainah" last="Sa'At">Zainah Sa'At</name>
<name sortKey="Suppiah, Jeyanthi" sort="Suppiah, Jeyanthi" uniqKey="Suppiah J" first="Jeyanthi" last="Suppiah">Jeyanthi Suppiah</name>
<name sortKey="Thayan, Ravindran" sort="Thayan, Ravindran" uniqKey="Thayan R" first="Ravindran" last="Thayan">Ravindran Thayan</name>
<name sortKey="Yusof, Apandi Mohd" sort="Yusof, Apandi Mohd" uniqKey="Yusof A" first="Apandi Mohd" last="Yusof">Apandi Mohd Yusof</name>
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