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Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia

Identifieur interne : 000A83 ( Pmc/Curation ); précédent : 000A82; suivant : 000A84

Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia

Auteurs : Nor-Aziyah Mat-Rahim ; Tengku Rogayah Tengku Abdul Rashid ; Jeyanthi Suppiah ; Ravindran Thayan ; Apandi Mohd Yusof ; Zainah Sa'At

Source :

Asian Pacific Journal of Tropical Disease [ 2222-1808 ] ; 2015.

RBID : PMC:7129196

Abstract

Objective

To describe the complete nucleocapsid (N) gene region of Middle East respiratory syndrome coronavirus (MERS-CoV) from imported case in Malaysia and the relations with human- and camel-derived MERS-CoV.

Methods

Combination of throat and nasal swab specimens was subjected to viral RNA extraction. For screening, the extracted RNA was subjected to real-time RT-PCR targeting upstream of E gene, open reading frame 1b and open reading frame 1a. For confirmation, the RNA was subjected to RT-PCR targeting partial part of the RNA-dependent RNA polymerase and nucleocapsid, followed by amplification of complete N gene region. Nucleotide sequencing of the first Malaysian case of MERS-CoV was performed following the confirmation with real-time RT-PCR detection.

Results

Initial analysis of partial RNA-dependent RNA polymerase and N gene revealed that the nucleotides had high similarity to Jeddah_1_2013 strain. Analysis of complete N gene region (1 242 nucleotides) from the case showed high similarity and yet distinct to the nucleotide sequences of camel-derived MERS-CoV.

Conclusions

From the finding, there are possibilities that the patient acquired the infection from zoonotic transmission from dromedary camels.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129196

DOI: 10.1016/S2222-1808(15)60833-7
PubMed: 32289031
PubMed Central: 7129196

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<author><name sortKey="Mat Rahim, Nor Aziyah" sort="Mat Rahim, Nor Aziyah" uniqKey="Mat Rahim N" first="Nor-Aziyah" last="Mat-Rahim">Nor-Aziyah Mat-Rahim</name>
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<author><name sortKey="Rashid, Tengku Rogayah Tengku Abdul" sort="Rashid, Tengku Rogayah Tengku Abdul" uniqKey="Rashid T" first="Tengku Rogayah Tengku Abdul" last="Rashid">Tengku Rogayah Tengku Abdul Rashid</name>
</author>
<author><name sortKey="Suppiah, Jeyanthi" sort="Suppiah, Jeyanthi" uniqKey="Suppiah J" first="Jeyanthi" last="Suppiah">Jeyanthi Suppiah</name>
</author>
<author><name sortKey="Thayan, Ravindran" sort="Thayan, Ravindran" uniqKey="Thayan R" first="Ravindran" last="Thayan">Ravindran Thayan</name>
</author>
<author><name sortKey="Yusof, Apandi Mohd" sort="Yusof, Apandi Mohd" uniqKey="Yusof A" first="Apandi Mohd" last="Yusof">Apandi Mohd Yusof</name>
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<author><name sortKey="Sa At, Zainah" sort="Sa At, Zainah" uniqKey="Sa At Z" first="Zainah" last="Sa'At">Zainah Sa'At</name>
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<author><name sortKey="Rashid, Tengku Rogayah Tengku Abdul" sort="Rashid, Tengku Rogayah Tengku Abdul" uniqKey="Rashid T" first="Tengku Rogayah Tengku Abdul" last="Rashid">Tengku Rogayah Tengku Abdul Rashid</name>
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<author><name sortKey="Suppiah, Jeyanthi" sort="Suppiah, Jeyanthi" uniqKey="Suppiah J" first="Jeyanthi" last="Suppiah">Jeyanthi Suppiah</name>
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<author><name sortKey="Thayan, Ravindran" sort="Thayan, Ravindran" uniqKey="Thayan R" first="Ravindran" last="Thayan">Ravindran Thayan</name>
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<author><name sortKey="Yusof, Apandi Mohd" sort="Yusof, Apandi Mohd" uniqKey="Yusof A" first="Apandi Mohd" last="Yusof">Apandi Mohd Yusof</name>
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<front><div type="abstract" xml:lang="en"><sec><title>Objective</title>
<p>To describe the complete nucleocapsid (<italic>N</italic>
) gene region of Middle East respiratory syndrome coronavirus (MERS-CoV) from imported case in Malaysia and the relations with human- and camel-derived MERS-CoV.</p>
</sec>
<sec><title>Methods</title>
<p>Combination of throat and nasal swab specimens was subjected to viral RNA extraction. For screening, the extracted RNA was subjected to real-time RT-PCR targeting upstream of <italic>E</italic>
 gene, open reading frame 1b and open reading frame 1a. For confirmation, the RNA was subjected to RT-PCR targeting partial part of the RNA-dependent RNA polymerase and nucleocapsid, followed by amplification of complete <italic>N</italic>
 gene region. Nucleotide sequencing of the first Malaysian case of MERS-CoV was performed following the confirmation with real-time RT-PCR detection.</p>
</sec>
<sec><title>Results</title>
<p>Initial analysis of partial RNA-dependent RNA polymerase and <italic>N</italic>
 gene revealed that the nucleotides had high similarity to Jeddah_1_2013 strain. Analysis of complete <italic>N</italic>
 gene region (1 242 nucleotides) from the case showed high similarity and yet distinct to the nucleotide sequences of camel-derived MERS-CoV.</p>
</sec>
<sec><title>Conclusions</title>
<p>From the finding, there are possibilities that the patient acquired the infection from zoonotic transmission from dromedary camels.</p>
</sec>
</div>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Asian Pac J Trop Dis</journal-id>
<journal-id journal-id-type="iso-abbrev">Asian Pac J Trop Dis</journal-id>
<journal-title-group><journal-title>Asian Pacific Journal of Tropical Disease</journal-title>
</journal-title-group>
<issn pub-type="epub">2222-1808</issn>
<publisher><publisher-name>Asian Pacific Tropical Medicine Press. Published by Elsevier (Singapore) Pte Ltd.</publisher-name>
</publisher>
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<article-meta><article-id pub-id-type="pmid">32289031</article-id>
<article-id pub-id-type="pmc">7129196</article-id>
<article-id pub-id-type="publisher-id">S2222-1808(15)60833-7</article-id>
<article-id pub-id-type="doi">10.1016/S2222-1808(15)60833-7</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" id="au10"><name><surname>Mat-Rahim</surname>
<given-names>Nor-Aziyah</given-names>
</name>
<email>aziyah@imr.gov.my</email>
<xref rid="cor1" ref-type="corresp">*</xref>
</contrib>
<contrib contrib-type="author" id="au20"><name><surname>Rashid</surname>
<given-names>Tengku Rogayah Tengku Abdul</given-names>
</name>
</contrib>
<contrib contrib-type="author" id="au30"><name><surname>Suppiah</surname>
<given-names>Jeyanthi</given-names>
</name>
</contrib>
<contrib contrib-type="author" id="au40"><name><surname>Thayan</surname>
<given-names>Ravindran</given-names>
</name>
</contrib>
<contrib contrib-type="author" id="au50"><name><surname>Yusof</surname>
<given-names>Apandi Mohd</given-names>
</name>
</contrib>
<contrib contrib-type="author" id="au60"><name><surname>Sa'at</surname>
<given-names>Zainah</given-names>
</name>
</contrib>
</contrib-group>
<aff id="aff1">Virology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia</aff>
<author-notes><corresp id="cor1"><label>*</label>
Corresponding author: Nor-Aziyah Mat-Rahim, Research Officer, Virology Unit, Institute for Medical Research, Jalan Pahang 50588 Kuala Lumpur, Malaysia <email>aziyah@imr.gov.my</email>
</corresp>
</author-notes>
<pub-date pub-type="pmc-release"><day>12</day>
<month>6</month>
<year>2015</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
      <pub-date pub-type="ppub"><month>7</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub"><day>12</day>
<month>6</month>
<year>2015</year>
</pub-date>
<volume>5</volume>
<issue>7</issue>
<fpage>543</fpage>
<lpage>546</lpage>
<history><date date-type="received"><day>13</day>
<month>1</month>
<year>2015</year>
</date>
<date date-type="rev-recd"><day>26</day>
<month>1</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>14</day>
<month>4</month>
<year>2015</year>
</date>
</history>
<permissions><copyright-statement>© 2015 Asian Pacific Tropical Medicine Press</copyright-statement>
<copyright-year>2015</copyright-year>
<license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p>
</license>
</permissions>
<abstract id="ceab10"><sec><title>Objective</title>
<p>To describe the complete nucleocapsid (<italic>N</italic>
) gene region of Middle East respiratory syndrome coronavirus (MERS-CoV) from imported case in Malaysia and the relations with human- and camel-derived MERS-CoV.</p>
</sec>
<sec><title>Methods</title>
<p>Combination of throat and nasal swab specimens was subjected to viral RNA extraction. For screening, the extracted RNA was subjected to real-time RT-PCR targeting upstream of <italic>E</italic>
 gene, open reading frame 1b and open reading frame 1a. For confirmation, the RNA was subjected to RT-PCR targeting partial part of the RNA-dependent RNA polymerase and nucleocapsid, followed by amplification of complete <italic>N</italic>
 gene region. Nucleotide sequencing of the first Malaysian case of MERS-CoV was performed following the confirmation with real-time RT-PCR detection.</p>
</sec>
<sec><title>Results</title>
<p>Initial analysis of partial RNA-dependent RNA polymerase and <italic>N</italic>
 gene revealed that the nucleotides had high similarity to Jeddah_1_2013 strain. Analysis of complete <italic>N</italic>
 gene region (1 242 nucleotides) from the case showed high similarity and yet distinct to the nucleotide sequences of camel-derived MERS-CoV.</p>
</sec>
<sec><title>Conclusions</title>
<p>From the finding, there are possibilities that the patient acquired the infection from zoonotic transmission from dromedary camels.</p>
</sec>
</abstract>
<kwd-group id="cekeyws10"><title>Keywords</title>
<kwd>Middle East respiratory syndrome coronavirus</kwd>
<kwd>Imported case</kwd>
<kwd>Malaysia</kwd>
<kwd>Nucleocapsid gene</kwd>
<kwd>Dromedary camels</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>

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Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia

Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia

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Abstract

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