MersV1, Main, Curation, bibRecord, 002649

Chemical synthesis of the mouse mitochondrial genome.

Identifieur interne : 002649 ( Main/Curation ); précédent : 002648; suivant : 002650

Chemical synthesis of the mouse mitochondrial genome.

Auteurs : Daniel G. Gibson [États-Unis] ; Hamilton O. Smith ; Clyde A. Hutchison ; J Craig Venter ; Chuck Merryman

Source :

Nature methods [ 1548-7105 ] ; 2010.

RBID : pubmed:20935651

Descripteurs français

KwdFr :
- ADN mitochondrial (synthèse chimique), Animaux, Chromosomes artificiels de bactérie, Génome mitochondrial, Recombinaison génétique, Réaction de polymérisation en chaîne, Souris.
MESH :
- synthèse chimique : ADN mitochondrial.
- Animaux, Chromosomes artificiels de bactérie, Génome mitochondrial, Recombinaison génétique, Réaction de polymérisation en chaîne, Souris.

English descriptors

KwdEn :
- Animals, Chromosomes, Artificial, Bacterial, DNA, Mitochondrial (chemical synthesis), Genome, Mitochondrial, Mice, Polymerase Chain Reaction, Recombination, Genetic.
MESH :
- chemical , chemical synthesis : DNA, Mitochondrial.
- Animals, Chromosomes, Artificial, Bacterial, Genome, Mitochondrial, Mice, Polymerase Chain Reaction, Recombination, Genetic.

Abstract

We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

DOI: 10.1038/nmeth.1515
PubMed: 20935651

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pubmed:20935651

Le document en format XML

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<author><name sortKey="Gibson, Daniel G" sort="Gibson, Daniel G" uniqKey="Gibson D" first="Daniel G" last="Gibson">Daniel G. Gibson</name>
<affiliation wicri:level="2"><nlm:affiliation>The J. Craig Venter Institute, Rockville, Maryland, USA. dgibson@jcvi.org</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The J. Craig Venter Institute, Rockville, Maryland</wicri:regionArea>
<placeName><region type="state">Maryland</region>
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<author><name sortKey="Smith, Hamilton O" sort="Smith, Hamilton O" uniqKey="Smith H" first="Hamilton O" last="Smith">Hamilton O. Smith</name>
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<author><name sortKey="Hutchison, Clyde A" sort="Hutchison, Clyde A" uniqKey="Hutchison C" first="Clyde A" last="Hutchison">Clyde A. Hutchison</name>
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<author><name sortKey="Venter, J Craig" sort="Venter, J Craig" uniqKey="Venter J" first="J Craig" last="Venter">J Craig Venter</name>
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<author><name sortKey="Merryman, Chuck" sort="Merryman, Chuck" uniqKey="Merryman C" first="Chuck" last="Merryman">Chuck Merryman</name>
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<affiliation wicri:level="2"><nlm:affiliation>The J. Craig Venter Institute, Rockville, Maryland, USA. dgibson@jcvi.org</nlm:affiliation>
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<placeName><region type="state">Maryland</region>
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<author><name sortKey="Smith, Hamilton O" sort="Smith, Hamilton O" uniqKey="Smith H" first="Hamilton O" last="Smith">Hamilton O. Smith</name>
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<author><name sortKey="Hutchison, Clyde A" sort="Hutchison, Clyde A" uniqKey="Hutchison C" first="Clyde A" last="Hutchison">Clyde A. Hutchison</name>
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<author><name sortKey="Venter, J Craig" sort="Venter, J Craig" uniqKey="Venter J" first="J Craig" last="Venter">J Craig Venter</name>
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<author><name sortKey="Merryman, Chuck" sort="Merryman, Chuck" uniqKey="Merryman C" first="Chuck" last="Merryman">Chuck Merryman</name>
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<series><title level="j">Nature methods</title>
<idno type="eISSN">1548-7105</idno>
<imprint><date when="2010" type="published">2010</date>
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<term>Genome, Mitochondrial</term>
<term>Mice</term>
<term>Polymerase Chain Reaction</term>
<term>Recombination, Genetic</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN mitochondrial (synthèse chimique)</term>
<term>Animaux</term>
<term>Chromosomes artificiels de bactérie</term>
<term>Génome mitochondrial</term>
<term>Recombinaison génétique</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Souris</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>DNA, Mitochondrial</term>
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<keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>ADN mitochondrial</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chromosomes, Artificial, Bacterial</term>
<term>Genome, Mitochondrial</term>
<term>Mice</term>
<term>Polymerase Chain Reaction</term>
<term>Recombination, Genetic</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Chromosomes artificiels de bactérie</term>
<term>Génome mitochondrial</term>
<term>Recombinaison génétique</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Souris</term>
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<front><div type="abstract" xml:lang="en">We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.</div>
</front>
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Chemical synthesis of the mouse mitochondrial genome.

Chemical synthesis of the mouse mitochondrial genome.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki