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Chemical synthesis of the mouse mitochondrial genome.

Identifieur interne : 001F30 ( PubMed/Corpus ); précédent : 001F29; suivant : 001F31

Chemical synthesis of the mouse mitochondrial genome.

Auteurs : Daniel G. Gibson ; Hamilton O. Smith ; Clyde A. Hutchison ; J Craig Venter ; Chuck Merryman

Source :

RBID : pubmed:20935651

English descriptors

Abstract

We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

DOI: 10.1038/nmeth.1515
PubMed: 20935651

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pubmed:20935651

Le document en format XML

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