Chemical synthesis of the mouse mitochondrial genome.
Identifieur interne : 001F30 ( PubMed/Corpus ); précédent : 001F29; suivant : 001F31Chemical synthesis of the mouse mitochondrial genome.
Auteurs : Daniel G. Gibson ; Hamilton O. Smith ; Clyde A. Hutchison ; J Craig Venter ; Chuck MerrymanSource :
- Nature methods [ 1548-7105 ] ; 2010.
English descriptors
- KwdEn :
- MESH :
- chemical , chemical synthesis : DNA, Mitochondrial.
- Animals, Chromosomes, Artificial, Bacterial, Genome, Mitochondrial, Mice, Polymerase Chain Reaction, Recombination, Genetic.
Abstract
We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.
DOI: 10.1038/nmeth.1515
PubMed: 20935651
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pubmed:20935651Le document en format XML
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<author><name sortKey="Venter, J Craig" sort="Venter, J Craig" uniqKey="Venter J" first="J Craig" last="Venter">J Craig Venter</name>
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<front><div type="abstract" xml:lang="en">We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.</div>
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