MersV1, PubMed, Corpus, bibRecord, 001F30

Chemical synthesis of the mouse mitochondrial genome.

Identifieur interne : 001F30 ( PubMed/Corpus ); précédent : 001F29; suivant : 001F31

Chemical synthesis of the mouse mitochondrial genome.

Auteurs : Daniel G. Gibson ; Hamilton O. Smith ; Clyde A. Hutchison ; J Craig Venter ; Chuck Merryman

Source :

Nature methods [ 1548-7105 ] ; 2010.

RBID : pubmed:20935651

English descriptors

KwdEn :
- Animals, Chromosomes, Artificial, Bacterial, DNA, Mitochondrial (chemical synthesis), Genome, Mitochondrial, Mice, Polymerase Chain Reaction, Recombination, Genetic.
MESH :
- chemical , chemical synthesis : DNA, Mitochondrial.
- Animals, Chromosomes, Artificial, Bacterial, Genome, Mitochondrial, Mice, Polymerase Chain Reaction, Recombination, Genetic.

Abstract

We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

DOI: 10.1038/nmeth.1515
PubMed: 20935651

Links to Exploration step

pubmed:20935651

Le document en format XML

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<author><name sortKey="Gibson, Daniel G" sort="Gibson, Daniel G" uniqKey="Gibson D" first="Daniel G" last="Gibson">Daniel G. Gibson</name>
<affiliation><nlm:affiliation>The J. Craig Venter Institute, Rockville, Maryland, USA. dgibson@jcvi.org</nlm:affiliation>
</affiliation>
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<author><name sortKey="Smith, Hamilton O" sort="Smith, Hamilton O" uniqKey="Smith H" first="Hamilton O" last="Smith">Hamilton O. Smith</name>
</author>
<author><name sortKey="Hutchison, Clyde A" sort="Hutchison, Clyde A" uniqKey="Hutchison C" first="Clyde A" last="Hutchison">Clyde A. Hutchison</name>
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<author><name sortKey="Venter, J Craig" sort="Venter, J Craig" uniqKey="Venter J" first="J Craig" last="Venter">J Craig Venter</name>
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<author><name sortKey="Merryman, Chuck" sort="Merryman, Chuck" uniqKey="Merryman C" first="Chuck" last="Merryman">Chuck Merryman</name>
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<author><name sortKey="Gibson, Daniel G" sort="Gibson, Daniel G" uniqKey="Gibson D" first="Daniel G" last="Gibson">Daniel G. Gibson</name>
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<author><name sortKey="Smith, Hamilton O" sort="Smith, Hamilton O" uniqKey="Smith H" first="Hamilton O" last="Smith">Hamilton O. Smith</name>
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<author><name sortKey="Hutchison, Clyde A" sort="Hutchison, Clyde A" uniqKey="Hutchison C" first="Clyde A" last="Hutchison">Clyde A. Hutchison</name>
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<author><name sortKey="Venter, J Craig" sort="Venter, J Craig" uniqKey="Venter J" first="J Craig" last="Venter">J Craig Venter</name>
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<author><name sortKey="Merryman, Chuck" sort="Merryman, Chuck" uniqKey="Merryman C" first="Chuck" last="Merryman">Chuck Merryman</name>
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<front><div type="abstract" xml:lang="en">We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.</div>
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<Title>Nature methods</Title>
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Chemical synthesis of the mouse mitochondrial genome.

Chemical synthesis of the mouse mitochondrial genome.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki