Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N(2)-dG adducts by capillary electrophoresis immunoassay.
Identifieur interne : 002616 ( Main/Curation ); précédent : 002615; suivant : 002617Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N(2)-dG adducts by capillary electrophoresis immunoassay.
Auteurs : Chao Wang [République populaire de Chine] ; Tao Li ; Zhixin Wang ; Feng Feng ; Hailin WangSource :
- Journal of chromatography. A [ 1873-3778 ] ; 2010.
Descripteurs français
- KwdFr :
- 7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol (), 7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol (analogues et dérivés), 7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol (métabolisme), Adduits à l'ADN (métabolisme), Anticorps monoclonaux (métabolisme), Dosage immunologique (), Désoxyguanosine (), Désoxyguanosine (analogues et dérivés), Désoxyguanosine (métabolisme), Fixation compétitive, Fluorescence, Génome humain (génétique), Humains, Lasers, Lignée cellulaire tumorale, Modèles linéaires, Rhodamines (métabolisme), Stéréoisomérie, Électrophorèse capillaire ().
- MESH :
- analogues et dérivés : 7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol, Désoxyguanosine.
- génétique : Génome humain.
- métabolisme : 7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol, Adduits à l'ADN, Anticorps monoclonaux, Désoxyguanosine, Rhodamines.
- 7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol, Dosage immunologique, Désoxyguanosine, Fixation compétitive, Fluorescence, Humains, Lasers, Lignée cellulaire tumorale, Modèles linéaires, Stéréoisomérie, Électrophorèse capillaire.
English descriptors
- KwdEn :
- 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (analogs & derivatives), 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (chemistry), 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (metabolism), Antibodies, Monoclonal (metabolism), Binding, Competitive, Cell Line, Tumor, DNA Adducts (metabolism), Deoxyguanosine (analogs & derivatives), Deoxyguanosine (chemistry), Deoxyguanosine (metabolism), Electrophoresis, Capillary (methods), Fluorescence, Genome, Human (genetics), Humans, Immunoassay (methods), Lasers, Linear Models, Rhodamines (metabolism), Stereoisomerism.
- MESH :
- chemical , analogs & derivatives : 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, Deoxyguanosine.
- chemical , chemistry : 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, Deoxyguanosine.
- chemical , metabolism : 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, Antibodies, Monoclonal, DNA Adducts, Deoxyguanosine, Rhodamines.
- genetics : Genome, Human.
- methods : Electrophoresis, Capillary, Immunoassay.
- Binding, Competitive, Cell Line, Tumor, Fluorescence, Humans, Lasers, Linear Models, Stereoisomerism.
Abstract
The stereospecific binding of monoclonal antibody (mAb) 8E11 to anti-benzo(a)pyrene diol epoxide (BPDE)-dG adducts in single nucleoside, long oligonucleotide, and genomic DNA were quantitatively evaluated using noncompetitive and competitive capillary electrophoresis (CE) immunoassays. Two single-stranded TMR-BPDE-90 mers containing a single anti-BPDE-dG adduct with defined stereochemistry and a fluorescent label at 5'-end were used as fluorescent probes for competitive CE immunoassay. To quantitatively evaluate the binding affinity through competitive CE immunoassays, a series of equations were derived according to the binding stoichiometry. The binding of mAb 8E11 to trans-(+)-anti-BPDE-dG displays strongest affinity (K(b): 3.57 x 10(8) M(-1)) among all four investigated anti-BPDE-dG mononucleoside adducts, and the cis-(-)-anti-BPDE-dG displays lowest affinity (K(b): 1.14 x10(7) M(-1)). The binding of monoclonal antibody (mAb) 8E11 to BPDE-dG adducts in long DNA (90 mer) preferentially forms the complex with a stoichiometry of 1:1, and that mAb 8E11 displays a slightly higher affinity with trans-(+)-anti-BPDE-90 mers (K(b): 6.36+/-0.54 x 10(8)M(-1)) than trans-(-)-anti-BPDE-90 mers (K(b): 4.52+/-0.52 x 10(8) M(-1)). The mAb 8E11 also displays high affinity with BPDE-dG adducts in genomic DNA (K(b): 3.74 x 10(8) M(-1)), indicating its promising applications for sensitive immuno-detection of BPDE-DNA adducts in genomic DNA.
DOI: 10.1016/j.chroma.2010.02.024
PubMed: 20223464
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001F63
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :001F63
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001E25
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000740
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :000740
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000740
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :002641
Links to Exploration step
pubmed:20223464Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N(2)-dG adducts by capillary electrophoresis immunoassay.</title>
<author><name sortKey="Wang, Chao" sort="Wang, Chao" uniqKey="Wang C" first="Chao" last="Wang">Chao Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Haidian Distr., Beijing 100085, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Haidian Distr., Beijing 100085</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Tao" sort="Li, Tao" uniqKey="Li T" first="Tao" last="Li">Tao Li</name>
</author>
<author><name sortKey="Wang, Zhixin" sort="Wang, Zhixin" uniqKey="Wang Z" first="Zhixin" last="Wang">Zhixin Wang</name>
</author>
<author><name sortKey="Feng, Feng" sort="Feng, Feng" uniqKey="Feng F" first="Feng" last="Feng">Feng Feng</name>
</author>
<author><name sortKey="Wang, Hailin" sort="Wang, Hailin" uniqKey="Wang H" first="Hailin" last="Wang">Hailin Wang</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2010">2010</date>
<idno type="RBID">pubmed:20223464</idno>
<idno type="pmid">20223464</idno>
<idno type="doi">10.1016/j.chroma.2010.02.024</idno>
<idno type="wicri:Area/PubMed/Corpus">001F63</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001F63</idno>
<idno type="wicri:Area/PubMed/Curation">001F63</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001F63</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001E25</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001E25</idno>
<idno type="wicri:Area/Ncbi/Merge">000740</idno>
<idno type="wicri:Area/Ncbi/Curation">000740</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000740</idno>
<idno type="wicri:Area/Main/Merge">002641</idno>
<idno type="wicri:Area/Main/Curation">002616</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N(2)-dG adducts by capillary electrophoresis immunoassay.</title>
<author><name sortKey="Wang, Chao" sort="Wang, Chao" uniqKey="Wang C" first="Chao" last="Wang">Chao Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Haidian Distr., Beijing 100085, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Haidian Distr., Beijing 100085</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Tao" sort="Li, Tao" uniqKey="Li T" first="Tao" last="Li">Tao Li</name>
</author>
<author><name sortKey="Wang, Zhixin" sort="Wang, Zhixin" uniqKey="Wang Z" first="Zhixin" last="Wang">Zhixin Wang</name>
</author>
<author><name sortKey="Feng, Feng" sort="Feng, Feng" uniqKey="Feng F" first="Feng" last="Feng">Feng Feng</name>
</author>
<author><name sortKey="Wang, Hailin" sort="Wang, Hailin" uniqKey="Wang H" first="Hailin" last="Wang">Hailin Wang</name>
</author>
</analytic>
<series><title level="j">Journal of chromatography. A</title>
<idno type="eISSN">1873-3778</idno>
<imprint><date when="2010" type="published">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (analogs & derivatives)</term>
<term>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (chemistry)</term>
<term>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (metabolism)</term>
<term>Antibodies, Monoclonal (metabolism)</term>
<term>Binding, Competitive</term>
<term>Cell Line, Tumor</term>
<term>DNA Adducts (metabolism)</term>
<term>Deoxyguanosine (analogs & derivatives)</term>
<term>Deoxyguanosine (chemistry)</term>
<term>Deoxyguanosine (metabolism)</term>
<term>Electrophoresis, Capillary (methods)</term>
<term>Fluorescence</term>
<term>Genome, Human (genetics)</term>
<term>Humans</term>
<term>Immunoassay (methods)</term>
<term>Lasers</term>
<term>Linear Models</term>
<term>Rhodamines (metabolism)</term>
<term>Stereoisomerism</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol ()</term>
<term>7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol (analogues et dérivés)</term>
<term>7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol (métabolisme)</term>
<term>Adduits à l'ADN (métabolisme)</term>
<term>Anticorps monoclonaux (métabolisme)</term>
<term>Dosage immunologique ()</term>
<term>Désoxyguanosine ()</term>
<term>Désoxyguanosine (analogues et dérivés)</term>
<term>Désoxyguanosine (métabolisme)</term>
<term>Fixation compétitive</term>
<term>Fluorescence</term>
<term>Génome humain (génétique)</term>
<term>Humains</term>
<term>Lasers</term>
<term>Lignée cellulaire tumorale</term>
<term>Modèles linéaires</term>
<term>Rhodamines (métabolisme)</term>
<term>Stéréoisomérie</term>
<term>Électrophorèse capillaire ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide</term>
<term>Deoxyguanosine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide</term>
<term>Deoxyguanosine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide</term>
<term>Antibodies, Monoclonal</term>
<term>DNA Adducts</term>
<term>Deoxyguanosine</term>
<term>Rhodamines</term>
</keywords>
<keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol</term>
<term>Désoxyguanosine</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genome, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Génome humain</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophoresis, Capillary</term>
<term>Immunoassay</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol</term>
<term>Adduits à l'ADN</term>
<term>Anticorps monoclonaux</term>
<term>Désoxyguanosine</term>
<term>Rhodamines</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Binding, Competitive</term>
<term>Cell Line, Tumor</term>
<term>Fluorescence</term>
<term>Humans</term>
<term>Lasers</term>
<term>Linear Models</term>
<term>Stereoisomerism</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol</term>
<term>Dosage immunologique</term>
<term>Désoxyguanosine</term>
<term>Fixation compétitive</term>
<term>Fluorescence</term>
<term>Humains</term>
<term>Lasers</term>
<term>Lignée cellulaire tumorale</term>
<term>Modèles linéaires</term>
<term>Stéréoisomérie</term>
<term>Électrophorèse capillaire</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The stereospecific binding of monoclonal antibody (mAb) 8E11 to anti-benzo(a)pyrene diol epoxide (BPDE)-dG adducts in single nucleoside, long oligonucleotide, and genomic DNA were quantitatively evaluated using noncompetitive and competitive capillary electrophoresis (CE) immunoassays. Two single-stranded TMR-BPDE-90 mers containing a single anti-BPDE-dG adduct with defined stereochemistry and a fluorescent label at 5'-end were used as fluorescent probes for competitive CE immunoassay. To quantitatively evaluate the binding affinity through competitive CE immunoassays, a series of equations were derived according to the binding stoichiometry. The binding of mAb 8E11 to trans-(+)-anti-BPDE-dG displays strongest affinity (K(b): 3.57 x 10(8) M(-1)) among all four investigated anti-BPDE-dG mononucleoside adducts, and the cis-(-)-anti-BPDE-dG displays lowest affinity (K(b): 1.14 x10(7) M(-1)). The binding of monoclonal antibody (mAb) 8E11 to BPDE-dG adducts in long DNA (90 mer) preferentially forms the complex with a stoichiometry of 1:1, and that mAb 8E11 displays a slightly higher affinity with trans-(+)-anti-BPDE-90 mers (K(b): 6.36+/-0.54 x 10(8)M(-1)) than trans-(-)-anti-BPDE-90 mers (K(b): 4.52+/-0.52 x 10(8) M(-1)). The mAb 8E11 also displays high affinity with BPDE-dG adducts in genomic DNA (K(b): 3.74 x 10(8) M(-1)), indicating its promising applications for sensitive immuno-detection of BPDE-DNA adducts in genomic DNA.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002616 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 002616 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Curation |type= RBID |clé= pubmed:20223464 |texte= Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N(2)-dG adducts by capillary electrophoresis immunoassay. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:20223464" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
This area was generated with Dilib version V0.6.33. |