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Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N(2)-dG adducts by capillary electrophoresis immunoassay.

Identifieur interne : 001E25 ( PubMed/Checkpoint ); précédent : 001E24; suivant : 001E26

Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N(2)-dG adducts by capillary electrophoresis immunoassay.

Auteurs : Chao Wang [République populaire de Chine] ; Tao Li ; Zhixin Wang ; Feng Feng ; Hailin Wang

Source :

Journal of chromatography. A [ 1873-3778 ] ; 2010.

RBID : pubmed:20223464

Descripteurs français

English descriptors

KwdEn :
- 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (analogs & derivatives), 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (chemistry), 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (metabolism), Antibodies, Monoclonal (metabolism), Binding, Competitive, Cell Line, Tumor, DNA Adducts (metabolism), Deoxyguanosine (analogs & derivatives), Deoxyguanosine (chemistry), Deoxyguanosine (metabolism), Electrophoresis, Capillary (methods), Fluorescence, Genome, Human (genetics), Humans, Immunoassay (methods), Lasers, Linear Models, Rhodamines (metabolism), Stereoisomerism.
MESH :
- chemical , analogs & derivatives : 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, Deoxyguanosine.
- chemical , chemistry : 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, Deoxyguanosine.
- chemical , metabolism : 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, Antibodies, Monoclonal, DNA Adducts, Deoxyguanosine, Rhodamines.
- genetics : Genome, Human.
- methods : Electrophoresis, Capillary, Immunoassay.
- Binding, Competitive, Cell Line, Tumor, Fluorescence, Humans, Lasers, Linear Models, Stereoisomerism.

Abstract

The stereospecific binding of monoclonal antibody (mAb) 8E11 to anti-benzo(a)pyrene diol epoxide (BPDE)-dG adducts in single nucleoside, long oligonucleotide, and genomic DNA were quantitatively evaluated using noncompetitive and competitive capillary electrophoresis (CE) immunoassays. Two single-stranded TMR-BPDE-90 mers containing a single anti-BPDE-dG adduct with defined stereochemistry and a fluorescent label at 5'-end were used as fluorescent probes for competitive CE immunoassay. To quantitatively evaluate the binding affinity through competitive CE immunoassays, a series of equations were derived according to the binding stoichiometry. The binding of mAb 8E11 to trans-(+)-anti-BPDE-dG displays strongest affinity (K(b): 3.57 x 10(8) M(-1)) among all four investigated anti-BPDE-dG mononucleoside adducts, and the cis-(-)-anti-BPDE-dG displays lowest affinity (K(b): 1.14 x10(7) M(-1)). The binding of monoclonal antibody (mAb) 8E11 to BPDE-dG adducts in long DNA (90 mer) preferentially forms the complex with a stoichiometry of 1:1, and that mAb 8E11 displays a slightly higher affinity with trans-(+)-anti-BPDE-90 mers (K(b): 6.36+/-0.54 x 10(8)M(-1)) than trans-(-)-anti-BPDE-90 mers (K(b): 4.52+/-0.52 x 10(8) M(-1)). The mAb 8E11 also displays high affinity with BPDE-dG adducts in genomic DNA (K(b): 3.74 x 10(8) M(-1)), indicating its promising applications for sensitive immuno-detection of BPDE-DNA adducts in genomic DNA.

DOI: 10.1016/j.chroma.2010.02.024
PubMed: 20223464

Affiliations:

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<term>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (chemistry)</term>
<term>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (metabolism)</term>
<term>Antibodies, Monoclonal (metabolism)</term>
<term>Binding, Competitive</term>
<term>Cell Line, Tumor</term>
<term>DNA Adducts (metabolism)</term>
<term>Deoxyguanosine (analogs & derivatives)</term>
<term>Deoxyguanosine (chemistry)</term>
<term>Deoxyguanosine (metabolism)</term>
<term>Electrophoresis, Capillary (methods)</term>
<term>Fluorescence</term>
<term>Genome, Human (genetics)</term>
<term>Humans</term>
<term>Immunoassay (methods)</term>
<term>Lasers</term>
<term>Linear Models</term>
<term>Rhodamines (metabolism)</term>
<term>Stereoisomerism</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol ()</term>
<term>7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol (analogues et dérivés)</term>
<term>7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol (métabolisme)</term>
<term>Adduits à l'ADN (métabolisme)</term>
<term>Anticorps monoclonaux (métabolisme)</term>
<term>Dosage immunologique ()</term>
<term>Désoxyguanosine ()</term>
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<term>Fluorescence</term>
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<term>Lignée cellulaire tumorale</term>
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<term>Rhodamines (métabolisme)</term>
<term>Stéréoisomérie</term>
<term>Électrophorèse capillaire ()</term>
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<term>Deoxyguanosine</term>
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<term>Antibodies, Monoclonal</term>
<term>DNA Adducts</term>
<term>Deoxyguanosine</term>
<term>Rhodamines</term>
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<term>Désoxyguanosine</term>
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<term>Linear Models</term>
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<term>Désoxyguanosine</term>
<term>Fixation compétitive</term>
<term>Fluorescence</term>
<term>Humains</term>
<term>Lasers</term>
<term>Lignée cellulaire tumorale</term>
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<front><div type="abstract" xml:lang="en">The stereospecific binding of monoclonal antibody (mAb) 8E11 to anti-benzo(a)pyrene diol epoxide (BPDE)-dG adducts in single nucleoside, long oligonucleotide, and genomic DNA were quantitatively evaluated using noncompetitive and competitive capillary electrophoresis (CE) immunoassays. Two single-stranded TMR-BPDE-90 mers containing a single anti-BPDE-dG adduct with defined stereochemistry and a fluorescent label at 5'-end were used as fluorescent probes for competitive CE immunoassay. To quantitatively evaluate the binding affinity through competitive CE immunoassays, a series of equations were derived according to the binding stoichiometry. The binding of mAb 8E11 to trans-(+)-anti-BPDE-dG displays strongest affinity (K(b): 3.57 x 10(8) M(-1)) among all four investigated anti-BPDE-dG mononucleoside adducts, and the cis-(-)-anti-BPDE-dG displays lowest affinity (K(b): 1.14 x10(7) M(-1)). The binding of monoclonal antibody (mAb) 8E11 to BPDE-dG adducts in long DNA (90 mer) preferentially forms the complex with a stoichiometry of 1:1, and that mAb 8E11 displays a slightly higher affinity with trans-(+)-anti-BPDE-90 mers (K(b): 6.36+/-0.54 x 10(8)M(-1)) than trans-(-)-anti-BPDE-90 mers (K(b): 4.52+/-0.52 x 10(8) M(-1)). The mAb 8E11 also displays high affinity with BPDE-dG adducts in genomic DNA (K(b): 3.74 x 10(8) M(-1)), indicating its promising applications for sensitive immuno-detection of BPDE-DNA adducts in genomic DNA.</div>
</front>
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<Abstract><AbstractText>The stereospecific binding of monoclonal antibody (mAb) 8E11 to anti-benzo(a)pyrene diol epoxide (BPDE)-dG adducts in single nucleoside, long oligonucleotide, and genomic DNA were quantitatively evaluated using noncompetitive and competitive capillary electrophoresis (CE) immunoassays. Two single-stranded TMR-BPDE-90 mers containing a single anti-BPDE-dG adduct with defined stereochemistry and a fluorescent label at 5'-end were used as fluorescent probes for competitive CE immunoassay. To quantitatively evaluate the binding affinity through competitive CE immunoassays, a series of equations were derived according to the binding stoichiometry. The binding of mAb 8E11 to trans-(+)-anti-BPDE-dG displays strongest affinity (K(b): 3.57 x 10(8) M(-1)) among all four investigated anti-BPDE-dG mononucleoside adducts, and the cis-(-)-anti-BPDE-dG displays lowest affinity (K(b): 1.14 x10(7) M(-1)). The binding of monoclonal antibody (mAb) 8E11 to BPDE-dG adducts in long DNA (90 mer) preferentially forms the complex with a stoichiometry of 1:1, and that mAb 8E11 displays a slightly higher affinity with trans-(+)-anti-BPDE-90 mers (K(b): 6.36+/-0.54 x 10(8)M(-1)) than trans-(-)-anti-BPDE-90 mers (K(b): 4.52+/-0.52 x 10(8) M(-1)). The mAb 8E11 also displays high affinity with BPDE-dG adducts in genomic DNA (K(b): 3.74 x 10(8) M(-1)), indicating its promising applications for sensitive immuno-detection of BPDE-DNA adducts in genomic DNA.</AbstractText>
<CopyrightInformation>Copyright 2010 Elsevier B.V. All rights reserved.</CopyrightInformation>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Chao</ForeName>
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   |étape=   Checkpoint
   |type=    RBID
   |clé=     pubmed:20223464
   |texte=   Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N(2)-dG adducts by capillary electrophoresis immunoassay.
}}

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HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i   -Sk "pubmed:20223464" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

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Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N(2)-dG adducts by capillary electrophoresis immunoassay.

Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N(2)-dG adducts by capillary electrophoresis immunoassay.

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