Chromatin structure of Schizosaccharomyces pombe
Identifieur interne : 002649 ( Istex/Curation ); précédent : 002648; suivant : 002650Chromatin structure of Schizosaccharomyces pombe
Auteurs : J. S. Godde [États-Unis] ; J. Widom [États-Unis]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 1992.
English descriptors
- KwdEn :
- Teeft :
- Acad, Agarose, Average length, Band number, Beckman rotor, Biochemistry, Biol, Cell biol, Cell types, Cerevisiae, Chicken erythrocyte chromatin, Chicken erythrocyte nuclei, Chromatin, Chromatin fibers, Chromatin structure, Chromatosome, Chromosome, Chromosome structure, Core histones, Core particle, Core particles, Crosslinking, Densitometer tracings, Digestion, Erard barker, Error bars, Erythrocyte, Eukaryote, Extensive digestion, Filament, Finch, Further digestion, Godde, Helical periodicity, Higher cells, Higher order structure, Histone, Holde, Holde yager, Klug, Kornberg, Linker, Linker model, Liquid nitrogen, Lowary, Lowary widom, Micrococcal, Micrococcal nuclease, Micrococcal nuclease digestion, Minimum unit, Molecular biology, Molecular weight, Monomer, Monomer length, Monomer particles, Native chromatin structure, Nidulans, Nuclease, Nuclease digestion, Nucleosome, Nucleosome core particle, Nucleosome filament, Nucleosome oligomers, Nucleosomes, Oligomers, Oligonucleosomal bands, Polizzi clarke, Polyoma virus, Pombe, Pombe cells, Pombe chromatin, Pombe genome, Proc, Protease, Protease inhibitors, Rearrangement, Size standards, Spheroplasted, Spheroplasting, Structural rearrangements, Subunit, Units micrococcal nuclease, Waring blender, Watkins smerdon, Weischet, Widom, Yanagida, Yeast, Yeast dextrose, Zymolyase.
Abstract
We have used new methods for chromatin isolation, together with conventional methods for measuring the nucleosome repeat length, to determine the repeat length of Schizosaccharomyces pombe chromatin. We obtain a result of 156(±2) bp. Equivalent results are obtained using a psoralen crosslinking method for measuring the repeat length in viable spheroplasts. That result, together with other control experiments, rules out many possible artifacts. The measured value of 156(±2) bp is smaller than the length of DNA found in the chromatosome. Thus, the chromatosome cannot be the fundamental unit of chromatin structure in all eukaryotes. The crossed linker model of chromatin higher order structure is incompatible with a nucleosome repeat length of 156 bp, and thus cannot apply to all eukaryotes. The solenoid model of higher order structure is compatible with this repeat length only if the solenoid is right-handed. We note two other properties of this chromatin. (1) Early in digestion, the DNA length of mononucleosomes from S. pombe and Aspergillus nidulans exceeds the nucleosome repeat length. (2) Many methods for isolating chromatin from S. pombe yield an apparent nucleosome repeat length of ≤ 140 bp; this result is found to be an artifactual consequence of nucleosome sliding.
Url:
DOI: 10.1016/0022-2836(92)91049-U
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Godde</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Illinois Urbana, IL 61801, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, University of Illinois Urbana, IL 61801</wicri:regionArea></affiliation><affiliation wicri:level="2"><mods:affiliation>†Present address: Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208-3500 U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>†Present address: Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston</wicri:cityArea></affiliation></author><author><name sortKey="Widom, J" sort="Widom, J" uniqKey="Widom J" first="J." last="Widom">J. 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S." last="Godde">J. S. Godde</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Illinois Urbana, IL 61801, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, University of Illinois Urbana, IL 61801</wicri:regionArea></affiliation><affiliation wicri:level="2"><mods:affiliation>†Present address: Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208-3500 U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>†Present address: Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston</wicri:cityArea></affiliation></author><author><name sortKey="Widom, J" sort="Widom, J" uniqKey="Widom J" first="J." last="Widom">J. Widom</name><affiliation><mods:affiliation>To whom all correspondence should be addressed.</mods:affiliation></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, Molecular Biology and Cell Biology, and Department of Chemistry, Northwestern University Evanston, IL 60208-3500, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, Molecular Biology and Cell Biology, and Department of Chemistry, Northwestern University Evanston, IL 60208-3500</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Molecular Biology</title><title level="j" type="abbrev">YJMBI</title><idno type="ISSN">0022-2836</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">226</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="1009">1009</biblScope><biblScope unit="page" to="1025">1025</biblScope></imprint><idno type="ISSN">0022-2836</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-2836</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>A. nidulans</term><term>S. pombe</term><term>TMP</term><term>bp</term><term>chromatin</term><term>chromatosome</term><term>nucleosome repeat length</term><term>u.v.</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Agarose</term><term>Average length</term><term>Band number</term><term>Beckman rotor</term><term>Biochemistry</term><term>Biol</term><term>Cell biol</term><term>Cell types</term><term>Cerevisiae</term><term>Chicken erythrocyte chromatin</term><term>Chicken erythrocyte nuclei</term><term>Chromatin</term><term>Chromatin fibers</term><term>Chromatin structure</term><term>Chromatosome</term><term>Chromosome</term><term>Chromosome structure</term><term>Core histones</term><term>Core particle</term><term>Core particles</term><term>Crosslinking</term><term>Densitometer tracings</term><term>Digestion</term><term>Erard barker</term><term>Error bars</term><term>Erythrocyte</term><term>Eukaryote</term><term>Extensive digestion</term><term>Filament</term><term>Finch</term><term>Further digestion</term><term>Godde</term><term>Helical periodicity</term><term>Higher cells</term><term>Higher order structure</term><term>Histone</term><term>Holde</term><term>Holde yager</term><term>Klug</term><term>Kornberg</term><term>Linker</term><term>Linker model</term><term>Liquid nitrogen</term><term>Lowary</term><term>Lowary widom</term><term>Micrococcal</term><term>Micrococcal nuclease</term><term>Micrococcal nuclease digestion</term><term>Minimum unit</term><term>Molecular biology</term><term>Molecular weight</term><term>Monomer</term><term>Monomer length</term><term>Monomer particles</term><term>Native chromatin structure</term><term>Nidulans</term><term>Nuclease</term><term>Nuclease digestion</term><term>Nucleosome</term><term>Nucleosome core particle</term><term>Nucleosome filament</term><term>Nucleosome oligomers</term><term>Nucleosomes</term><term>Oligomers</term><term>Oligonucleosomal bands</term><term>Polizzi clarke</term><term>Polyoma virus</term><term>Pombe</term><term>Pombe cells</term><term>Pombe chromatin</term><term>Pombe genome</term><term>Proc</term><term>Protease</term><term>Protease inhibitors</term><term>Rearrangement</term><term>Size standards</term><term>Spheroplasted</term><term>Spheroplasting</term><term>Structural rearrangements</term><term>Subunit</term><term>Units micrococcal nuclease</term><term>Waring blender</term><term>Watkins smerdon</term><term>Weischet</term><term>Widom</term><term>Yanagida</term><term>Yeast</term><term>Yeast dextrose</term><term>Zymolyase</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have used new methods for chromatin isolation, together with conventional methods for measuring the nucleosome repeat length, to determine the repeat length of Schizosaccharomyces pombe chromatin. We obtain a result of 156(±2) bp. Equivalent results are obtained using a psoralen crosslinking method for measuring the repeat length in viable spheroplasts. That result, together with other control experiments, rules out many possible artifacts. The measured value of 156(±2) bp is smaller than the length of DNA found in the chromatosome. Thus, the chromatosome cannot be the fundamental unit of chromatin structure in all eukaryotes. The crossed linker model of chromatin higher order structure is incompatible with a nucleosome repeat length of 156 bp, and thus cannot apply to all eukaryotes. The solenoid model of higher order structure is compatible with this repeat length only if the solenoid is right-handed. We note two other properties of this chromatin. (1) Early in digestion, the DNA length of mononucleosomes from S. pombe and Aspergillus nidulans exceeds the nucleosome repeat length. (2) Many methods for isolating chromatin from S. pombe yield an apparent nucleosome repeat length of ≤ 140 bp; this result is found to be an artifactual consequence of nucleosome sliding.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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