Electron microscopy of size and shape of viral DNA in solutions of different ionic strengths
Identifieur interne : 002648 ( Istex/Curation ); précédent : 002647; suivant : 002649Electron microscopy of size and shape of viral DNA in solutions of different ionic strengths
Auteurs : D. Lang [États-Unis] ; H. Bujard [États-Unis] ; B. Wolff [États-Unis] ; D. Russell [États-Unis]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 1967.
English descriptors
- Teeft :
- Acad, Acta, Adsorption, Adsorption plane, Ammonium acetate, Average coil, Average conformation, Biol, Biological variability, Biophys, Bovine papilloma virus, Bulk solution, Calibration slide, Coil, Coil volume, Conformation, Contour, Contour length, Contour lengths, Cytochrome, Diff, Different contour lengths, Diffusion experiments, Diffusion method, Distance distribution, Distance distributions, Double helix, Electron microscopy, Error limits, Error sources, First compartment, Freifelder, Freifelder kleinschmidt, Glass slide, Grid, Hall cavalieri, Inman, Intact molecule, Ionic, Ionic strength, Ionic strengths, Kaiser inman, Kleinschmidt, Kleinschmidt zahn, Lang, Langmuir trough, Length distributions, Length interval, Length measurements, Linear expansion, Linear extension, Machattie, Micrographs, Molecular weight, Molecular weights, Molecule, Monolayer, Nucleic acid molecules, Nucleic acids, Papilloma, Parallel projection, Pergamon press, Phage, Phenol, Pincushion distortion, Protein film, Protein surface film, Random coil, Random coils, Salt concentration, Salt concentrations, Salt solution, Shearing forces, Shope papilloma virus, Sodium chloride solution, Specimen grids, Square root, Standard deviation, Standard deviations, Statistical element, Stoeckenius, Surface film, Tangential orientation, Theoretical distribution, Zahn.
Abstract
Diffusion-controlled adsorption of DNA from bulk solution onto a monolayer of surface-denatured cytochrome c has been used to characterize, by electron microscopy, the size and shape of single DNA molecules from bacteriophages T3 and T1 and from bovine papilloma virus. For ionic strengths, I ⩾ 0·14, the following DNA contour lengths and sample standard deviations are found: T3, (11·6 ± 0·4) μ; T1, (16·0 ± 1·0) μ and bovine papilloma virus (circular), (2·45 ± 0·07) μ. For DNA solutions with I < 0·1, the contour length of the adsorbed DNA increases up to 20% with decreasing I, presumably by a uniform, partial unwinding of the B configuration. Within the experimental error (standard deviation = ± 4%), the DNA contour lengths are found equal, whether (a) perchlorate or phenol extraction, (b) diffusion or spreading methods, or (c) ammonium acetate or sodium chloride as added salts were used. Based on experimental and operational values of standard deviation, variability of natural contour length of intact T3 or bovine papilloma virus DNA must be less than about ± 2%, if there is any. The conformation of intact T3 DNA, as measured by the distribution of end-to-end distances with the diffusion method, has been identical, at I = 0·4, within the error limits, with the calculated theoretical distribution for random coils, with small increasing deviations at 0·15 and 0·05 ionic strength.
Url:
DOI: 10.1016/S0022-2836(67)80024-X
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Lang</name><affiliation wicri:level="1"><mods:affiliation>Division of Biology Southwest Center for Advanced Studies Dallas, Texas, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Biology Southwest Center for Advanced Studies Dallas, Texas</wicri:regionArea></affiliation></author><author><name sortKey="Bujard, H" sort="Bujard, H" uniqKey="Bujard H" first="H." last="Bujard">H. Bujard</name><affiliation wicri:level="1"><mods:affiliation>Division of Biology Southwest Center for Advanced Studies Dallas, Texas, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Biology Southwest Center for Advanced Studies Dallas, Texas</wicri:regionArea></affiliation></author><author><name sortKey="Wolff, B" sort="Wolff, B" uniqKey="Wolff B" first="B." last="Wolff">B. Wolff</name><affiliation wicri:level="1"><mods:affiliation>Division of Biology Southwest Center for Advanced Studies Dallas, Texas, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Biology Southwest Center for Advanced Studies Dallas, Texas</wicri:regionArea></affiliation></author><author><name sortKey="Russell, D" sort="Russell, D" uniqKey="Russell D" first="D." last="Russell">D. Russell</name><affiliation wicri:level="1"><mods:affiliation>Division of Biology Southwest Center for Advanced Studies Dallas, Texas, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Biology Southwest Center for Advanced Studies Dallas, Texas</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Molecular Biology</title><title level="j" type="abbrev">YJMBI</title><idno type="ISSN">0022-2836</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1967">1967</date><biblScope unit="volume">23</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="163">163</biblScope><biblScope unit="page" to="166">166</biblScope></imprint><idno type="ISSN">0022-2836</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-2836</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Acta</term><term>Adsorption</term><term>Adsorption plane</term><term>Ammonium acetate</term><term>Average coil</term><term>Average conformation</term><term>Biol</term><term>Biological variability</term><term>Biophys</term><term>Bovine papilloma virus</term><term>Bulk solution</term><term>Calibration slide</term><term>Coil</term><term>Coil volume</term><term>Conformation</term><term>Contour</term><term>Contour length</term><term>Contour lengths</term><term>Cytochrome</term><term>Diff</term><term>Different contour lengths</term><term>Diffusion experiments</term><term>Diffusion method</term><term>Distance distribution</term><term>Distance distributions</term><term>Double helix</term><term>Electron microscopy</term><term>Error limits</term><term>Error sources</term><term>First compartment</term><term>Freifelder</term><term>Freifelder kleinschmidt</term><term>Glass slide</term><term>Grid</term><term>Hall cavalieri</term><term>Inman</term><term>Intact molecule</term><term>Ionic</term><term>Ionic strength</term><term>Ionic strengths</term><term>Kaiser inman</term><term>Kleinschmidt</term><term>Kleinschmidt zahn</term><term>Lang</term><term>Langmuir trough</term><term>Length distributions</term><term>Length interval</term><term>Length measurements</term><term>Linear expansion</term><term>Linear extension</term><term>Machattie</term><term>Micrographs</term><term>Molecular weight</term><term>Molecular weights</term><term>Molecule</term><term>Monolayer</term><term>Nucleic acid molecules</term><term>Nucleic acids</term><term>Papilloma</term><term>Parallel projection</term><term>Pergamon press</term><term>Phage</term><term>Phenol</term><term>Pincushion distortion</term><term>Protein film</term><term>Protein surface film</term><term>Random coil</term><term>Random coils</term><term>Salt concentration</term><term>Salt concentrations</term><term>Salt solution</term><term>Shearing forces</term><term>Shope papilloma virus</term><term>Sodium chloride solution</term><term>Specimen grids</term><term>Square root</term><term>Standard deviation</term><term>Standard deviations</term><term>Statistical element</term><term>Stoeckenius</term><term>Surface film</term><term>Tangential orientation</term><term>Theoretical distribution</term><term>Zahn</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Diffusion-controlled adsorption of DNA from bulk solution onto a monolayer of surface-denatured cytochrome c has been used to characterize, by electron microscopy, the size and shape of single DNA molecules from bacteriophages T3 and T1 and from bovine papilloma virus. For ionic strengths, I ⩾ 0·14, the following DNA contour lengths and sample standard deviations are found: T3, (11·6 ± 0·4) μ; T1, (16·0 ± 1·0) μ and bovine papilloma virus (circular), (2·45 ± 0·07) μ. For DNA solutions with I < 0·1, the contour length of the adsorbed DNA increases up to 20% with decreasing I, presumably by a uniform, partial unwinding of the B configuration. Within the experimental error (standard deviation = ± 4%), the DNA contour lengths are found equal, whether (a) perchlorate or phenol extraction, (b) diffusion or spreading methods, or (c) ammonium acetate or sodium chloride as added salts were used. Based on experimental and operational values of standard deviation, variability of natural contour length of intact T3 or bovine papilloma virus DNA must be less than about ± 2%, if there is any. The conformation of intact T3 DNA, as measured by the distribution of end-to-end distances with the diffusion method, has been identical, at I = 0·4, within the error limits, with the calculated theoretical distribution for random coils, with small increasing deviations at 0·15 and 0·05 ionic strength.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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