Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines
Identifieur interne : 002620 ( Istex/Curation ); précédent : 002619; suivant : 002621Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines
Auteurs : Anne Kjerrström [Suède] ; Jorma Hinkula [Suède] ; Gunnel Engström [Suède] ; Vladimir Ovod [Finlande] ; Kai Krohn [Finlande] ; Reinhold Benthin [Suède] ; Britta Wahren [Suède]Source :
- Virology [ 0042-6822 ] ; 2001.
English descriptors
- Teeft :
- Absorbence, Absorbence value, Assay, Cell debris, Cell supernatants, Cellular responses, Citric acid, Color development, Combination formula, Complete antibody response, Control antigen, Deionized water, Elisa, Empty vector, Empty vector pkcmv, Encoding, Epitope, Epitope mapping, Epitope pattern, Epitope reactivity, Fetal calf serum, Fugene, Full speed, Gene, Gene administration, Gene expression, Hcmvtat, Hela, Hela cells, Hela cells transfected, Hinkula, Human immunodeficiency virus type, Humoral, Immune, Immune responses, Immunity humoral responses, Immunization, Immunodeficiency, Immunogen, Immunogenicity, Immunostimulatory, Kjerrstrom, Life technologies, Linear regression analysis, Major histocompatibility, Mouse, Nalge nunc, Negative controls, Pbluescript, Peptide, Peptide responses, Pkcmv, Pkcmvnef, Pkcmvrev, Pkcmvtat, Plasmid, Plasmid pbrev, Plasmids encoding, Polya, Polya signal, Polyclonal, Positive control, Promoter, Protein expression, Protein expression capacity, Reagent, Recombinant, Reference wavelength, Regulatory genes, Regulatory proteins, Representative data, Representative peptides, Respective antigen, Respective plasmid, Responder, Rnef, Roche, Roche diagnostics corp, Rrev, Rtat, Separate tubes, Several experiments, Single gene, Single genes, Sodium bicarbonate, Standard curve, Standard errors, Subclass, Subclass determination, Sulfuric acid, Transfected, Transfected cells, Transfection, Transfection reagent, Triton, Tween, Untransfected cells, Vaccine, Viral protein, Virol.
Abstract
Abstract: DNA immunization permits evaluation of possible antagonistic or synergistic effects between the encoded components. The protein expression capacity in vitro was related to the immunogenicity in vivo of plasmids encoding the HIV-1 regulatory genes tat rev, and nef. Neither Tat nor Rev expression was influenced by co-expression in vitro of all three proteins, while Nef expression was slightly inhibited. With the combination of genes, the T-cellular responses of mice against Rev and Nef were inhibited compared with those when single gene immunization was used. No interference was detected for the Tat T-cell response. Thus, co-immunization with certain genes may result in inhibition of specific immune responses.
Url:
DOI: 10.1006/viro.2001.0905
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responses</term><term>Immunization</term><term>Immunodeficiency</term><term>Immunogen</term><term>Immunogenicity</term><term>Immunostimulatory</term><term>Kjerrstrom</term><term>Life technologies</term><term>Linear regression analysis</term><term>Major histocompatibility</term><term>Mouse</term><term>Nalge nunc</term><term>Negative controls</term><term>Pbluescript</term><term>Peptide</term><term>Peptide responses</term><term>Pkcmv</term><term>Pkcmvnef</term><term>Pkcmvrev</term><term>Pkcmvtat</term><term>Plasmid</term><term>Plasmid pbrev</term><term>Plasmids encoding</term><term>Polya</term><term>Polya signal</term><term>Polyclonal</term><term>Positive control</term><term>Promoter</term><term>Protein expression</term><term>Protein expression capacity</term><term>Reagent</term><term>Recombinant</term><term>Reference wavelength</term><term>Regulatory genes</term><term>Regulatory proteins</term><term>Representative data</term><term>Representative peptides</term><term>Respective antigen</term><term>Respective plasmid</term><term>Responder</term><term>Rnef</term><term>Roche</term><term>Roche diagnostics corp</term><term>Rrev</term><term>Rtat</term><term>Separate tubes</term><term>Several experiments</term><term>Single gene</term><term>Single genes</term><term>Sodium bicarbonate</term><term>Standard curve</term><term>Standard errors</term><term>Subclass</term><term>Subclass determination</term><term>Sulfuric acid</term><term>Transfected</term><term>Transfected cells</term><term>Transfection</term><term>Transfection reagent</term><term>Triton</term><term>Tween</term><term>Untransfected cells</term><term>Vaccine</term><term>Viral protein</term><term>Virol</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: DNA immunization permits evaluation of possible antagonistic or synergistic effects between the encoded components. The protein expression capacity in vitro was related to the immunogenicity in vivo of plasmids encoding the HIV-1 regulatory genes tat rev, and nef. Neither Tat nor Rev expression was influenced by co-expression in vitro of all three proteins, while Nef expression was slightly inhibited. With the combination of genes, the T-cellular responses of mice against Rev and Nef were inhibited compared with those when single gene immunization was used. No interference was detected for the Tat T-cell response. Thus, co-immunization with certain genes may result in inhibition of specific immune responses.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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