The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor
Identifieur interne : 002545 ( Istex/Curation ); précédent : 002544; suivant : 002546The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor
Auteurs : He Wang [États-Unis] ; Alan M. Lambowitz [États-Unis]Source :
- Cell [ 0092-8674 ] ; 1993.
English descriptors
- Teeft :
- Akins, Alkaline phosphatase, Biol, Cdna, Cdna primers, Cdna product, Cdna synthesis, Clone, Dgmp, Dgtp, Digestion, Eickbush, England biolabs, Experimental procedures, Initiation, Initiation reaction, Initiation site, Internal site, Intron, Kennell, Kinase, Kuiper, Lambowitz, Ligated, Mauriceville, Mauriceville plasmid, Mauriceville plasmid enzyme, Mitochondrial, Mitochondrion, Molecular weight markers, Neurospora, Nsil, Nucleotide, Oligonucleotide, Oligonucleotides, Pbluescript, Penultimate, Phosphatase, Plasmid, Plasmid transcript, Polyacrylamide, Polymerase, Polynucleotide, Polynucleotide kinase, Prime cdna synthesis, Primer, Protease, Reaction medium, Replication, Retroelements, Rnaase, Sau3al fragments, Standard reaction medium, Strand synthesis, Template, Transcript, Transcriptase, Transcription, Transcription reactions, Trna, Unpublished data, Urea, Wang.
Abstract
Abstract: We show that the reverse transcriptase (RT) encoded by the Mauriceville mitochondrial plasmid of Neurospora closely resembles viral RNA-dependent RNA polymerases in initiating cDNA synthesis opposite the penultimate C residue of a 3′ tRNA-like structure and has the unprecedented ability for a DNA polymerase to initiate DNA synthesis at a specific site in a natural template without a primer. The Mauriceville plasmid enzyme can also use DNA or RNA primers in a manner suggesting how a primitive RT could have evolved from an RNA-dependent RNA polymerase into retroviral and other types of RTs. The characteristics of the Mauriceville plasmid RT suggest that it may be related to the progenitor of present-day RTs and DNA polymerases.
Url:
DOI: 10.1016/0092-8674(93)90317-J
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002545
Links to Exploration step
ISTEX:CCCA4A5509EB1DF412D478F49D5CAFA5EA86708CLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor</title>
<author><name sortKey="Wang, He" sort="Wang, He" uniqKey="Wang H" first="He" last="Wang">He Wang</name>
<affiliation wicri:level="2"><mods:affiliation>Department of Molecular Genetics The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Molecular Genetics The Ohio State University Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><mods:affiliation>Department of Biochemistry The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Biochemistry The Ohio State University Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><mods:affiliation>Department of Medical Biochemistry The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Medical Biochemistry The Ohio State University Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><mods:affiliation>the Biotechnology Center The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>the Biotechnology Center The Ohio State University Columbus</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name>
<affiliation wicri:level="2"><mods:affiliation>Department of Molecular Genetics The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Molecular Genetics The Ohio State University Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><mods:affiliation>Department of Biochemistry The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Biochemistry The Ohio State University Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><mods:affiliation>Department of Medical Biochemistry The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Medical Biochemistry The Ohio State University Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><mods:affiliation>the Biotechnology Center The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>the Biotechnology Center The Ohio State University Columbus</wicri:cityArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:CCCA4A5509EB1DF412D478F49D5CAFA5EA86708C</idno>
<date when="1993" year="1993">1993</date>
<idno type="doi">10.1016/0092-8674(93)90317-J</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-RHKD81FR-N/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">002545</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002545</idno>
<idno type="wicri:Area/Istex/Curation">002545</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor</title>
<author><name sortKey="Wang, He" sort="Wang, He" uniqKey="Wang H" first="He" last="Wang">He Wang</name>
<affiliation wicri:level="2"><mods:affiliation>Department of Molecular Genetics The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Molecular Genetics The Ohio State University Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><mods:affiliation>Department of Biochemistry The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Biochemistry The Ohio State University Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><mods:affiliation>Department of Medical Biochemistry The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Medical Biochemistry The Ohio State University Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><mods:affiliation>the Biotechnology Center The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>the Biotechnology Center The Ohio State University Columbus</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name>
<affiliation wicri:level="2"><mods:affiliation>Department of Molecular Genetics The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Molecular Genetics The Ohio State University Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><mods:affiliation>Department of Biochemistry The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Biochemistry The Ohio State University Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><mods:affiliation>Department of Medical Biochemistry The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Medical Biochemistry The Ohio State University Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><mods:affiliation>the Biotechnology Center The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>the Biotechnology Center The Ohio State University Columbus</wicri:cityArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Cell</title>
<title level="j" type="abbrev">CELL</title>
<idno type="ISSN">0092-8674</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1993">1993</date>
<biblScope unit="volume">75</biblScope>
<biblScope unit="issue">6</biblScope>
<biblScope unit="page" from="1071">1071</biblScope>
<biblScope unit="page" to="1081">1081</biblScope>
</imprint>
<idno type="ISSN">0092-8674</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0092-8674</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Akins</term>
<term>Alkaline phosphatase</term>
<term>Biol</term>
<term>Cdna</term>
<term>Cdna primers</term>
<term>Cdna product</term>
<term>Cdna synthesis</term>
<term>Clone</term>
<term>Dgmp</term>
<term>Dgtp</term>
<term>Digestion</term>
<term>Eickbush</term>
<term>England biolabs</term>
<term>Experimental procedures</term>
<term>Initiation</term>
<term>Initiation reaction</term>
<term>Initiation site</term>
<term>Internal site</term>
<term>Intron</term>
<term>Kennell</term>
<term>Kinase</term>
<term>Kuiper</term>
<term>Lambowitz</term>
<term>Ligated</term>
<term>Mauriceville</term>
<term>Mauriceville plasmid</term>
<term>Mauriceville plasmid enzyme</term>
<term>Mitochondrial</term>
<term>Mitochondrion</term>
<term>Molecular weight markers</term>
<term>Neurospora</term>
<term>Nsil</term>
<term>Nucleotide</term>
<term>Oligonucleotide</term>
<term>Oligonucleotides</term>
<term>Pbluescript</term>
<term>Penultimate</term>
<term>Phosphatase</term>
<term>Plasmid</term>
<term>Plasmid transcript</term>
<term>Polyacrylamide</term>
<term>Polymerase</term>
<term>Polynucleotide</term>
<term>Polynucleotide kinase</term>
<term>Prime cdna synthesis</term>
<term>Primer</term>
<term>Protease</term>
<term>Reaction medium</term>
<term>Replication</term>
<term>Retroelements</term>
<term>Rnaase</term>
<term>Sau3al fragments</term>
<term>Standard reaction medium</term>
<term>Strand synthesis</term>
<term>Template</term>
<term>Transcript</term>
<term>Transcriptase</term>
<term>Transcription</term>
<term>Transcription reactions</term>
<term>Trna</term>
<term>Unpublished data</term>
<term>Urea</term>
<term>Wang</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: We show that the reverse transcriptase (RT) encoded by the Mauriceville mitochondrial plasmid of Neurospora closely resembles viral RNA-dependent RNA polymerases in initiating cDNA synthesis opposite the penultimate C residue of a 3′ tRNA-like structure and has the unprecedented ability for a DNA polymerase to initiate DNA synthesis at a specific site in a natural template without a primer. The Mauriceville plasmid enzyme can also use DNA or RNA primers in a manner suggesting how a primitive RT could have evolved from an RNA-dependent RNA polymerase into retroviral and other types of RTs. The characteristics of the Mauriceville plasmid RT suggest that it may be related to the progenitor of present-day RTs and DNA polymerases.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002545 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 002545 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:CCCA4A5509EB1DF412D478F49D5CAFA5EA86708C |texte= The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor }}
This area was generated with Dilib version V0.6.33. |