The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor
Identifieur interne : 002545 ( Istex/Corpus ); précédent : 002544; suivant : 002546The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor
Auteurs : He Wang ; Alan M. LambowitzSource :
- Cell [ 0092-8674 ] ; 1993.
English descriptors
- Teeft :
- Akins, Alkaline phosphatase, Biol, Cdna, Cdna primers, Cdna product, Cdna synthesis, Clone, Dgmp, Dgtp, Digestion, Eickbush, England biolabs, Experimental procedures, Initiation, Initiation reaction, Initiation site, Internal site, Intron, Kennell, Kinase, Kuiper, Lambowitz, Ligated, Mauriceville, Mauriceville plasmid, Mauriceville plasmid enzyme, Mitochondrial, Mitochondrion, Molecular weight markers, Neurospora, Nsil, Nucleotide, Oligonucleotide, Oligonucleotides, Pbluescript, Penultimate, Phosphatase, Plasmid, Plasmid transcript, Polyacrylamide, Polymerase, Polynucleotide, Polynucleotide kinase, Prime cdna synthesis, Primer, Protease, Reaction medium, Replication, Retroelements, Rnaase, Sau3al fragments, Standard reaction medium, Strand synthesis, Template, Transcript, Transcriptase, Transcription, Transcription reactions, Trna, Unpublished data, Urea, Wang.
Abstract
Abstract: We show that the reverse transcriptase (RT) encoded by the Mauriceville mitochondrial plasmid of Neurospora closely resembles viral RNA-dependent RNA polymerases in initiating cDNA synthesis opposite the penultimate C residue of a 3′ tRNA-like structure and has the unprecedented ability for a DNA polymerase to initiate DNA synthesis at a specific site in a natural template without a primer. The Mauriceville plasmid enzyme can also use DNA or RNA primers in a manner suggesting how a primitive RT could have evolved from an RNA-dependent RNA polymerase into retroviral and other types of RTs. The characteristics of the Mauriceville plasmid RT suggest that it may be related to the progenitor of present-day RTs and DNA polymerases.
Url:
DOI: 10.1016/0092-8674(93)90317-J
Links to Exploration step
ISTEX:CCCA4A5509EB1DF412D478F49D5CAFA5EA86708CLe document en format XML
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Lambowitz</name><affiliation><mods:affiliation>Department of Molecular Genetics The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Biochemistry The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Medical Biochemistry The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation></affiliation><affiliation><mods:affiliation>the Biotechnology Center The Ohio State University Columbus, Ohio 43210 USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="ISSN">0092-8674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">75</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1071">1071</biblScope><biblScope unit="page" to="1081">1081</biblScope></imprint><idno type="ISSN">0092-8674</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0092-8674</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Akins</term><term>Alkaline phosphatase</term><term>Biol</term><term>Cdna</term><term>Cdna primers</term><term>Cdna product</term><term>Cdna synthesis</term><term>Clone</term><term>Dgmp</term><term>Dgtp</term><term>Digestion</term><term>Eickbush</term><term>England biolabs</term><term>Experimental procedures</term><term>Initiation</term><term>Initiation reaction</term><term>Initiation site</term><term>Internal site</term><term>Intron</term><term>Kennell</term><term>Kinase</term><term>Kuiper</term><term>Lambowitz</term><term>Ligated</term><term>Mauriceville</term><term>Mauriceville plasmid</term><term>Mauriceville plasmid enzyme</term><term>Mitochondrial</term><term>Mitochondrion</term><term>Molecular weight markers</term><term>Neurospora</term><term>Nsil</term><term>Nucleotide</term><term>Oligonucleotide</term><term>Oligonucleotides</term><term>Pbluescript</term><term>Penultimate</term><term>Phosphatase</term><term>Plasmid</term><term>Plasmid transcript</term><term>Polyacrylamide</term><term>Polymerase</term><term>Polynucleotide</term><term>Polynucleotide kinase</term><term>Prime cdna synthesis</term><term>Primer</term><term>Protease</term><term>Reaction medium</term><term>Replication</term><term>Retroelements</term><term>Rnaase</term><term>Sau3al fragments</term><term>Standard reaction medium</term><term>Strand synthesis</term><term>Template</term><term>Transcript</term><term>Transcriptase</term><term>Transcription</term><term>Transcription reactions</term><term>Trna</term><term>Unpublished data</term><term>Urea</term><term>Wang</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We show that the reverse transcriptase (RT) encoded by the Mauriceville mitochondrial plasmid of Neurospora closely resembles viral RNA-dependent RNA polymerases in initiating cDNA synthesis opposite the penultimate C residue of a 3′ tRNA-like structure and has the unprecedented ability for a DNA polymerase to initiate DNA synthesis at a specific site in a natural template without a primer. The Mauriceville plasmid enzyme can also use DNA or RNA primers in a manner suggesting how a primitive RT could have evolved from an RNA-dependent RNA polymerase into retroviral and other types of RTs. The characteristics of the Mauriceville plasmid RT suggest that it may be related to the progenitor of present-day RTs and DNA polymerases.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>cdna</json:string><json:string>plasmid</json:string><json:string>mauriceville</json:string><json:string>primer</json:string><json:string>mauriceville plasmid</json:string><json:string>polymerase</json:string><json:string>dgtp</json:string><json:string>cdna synthesis</json:string><json:string>wang</json:string><json:string>lambowitz</json:string><json:string>transcriptase</json:string><json:string>oligonucleotide</json:string><json:string>trna</json:string><json:string>transcription reactions</json:string><json:string>retroelements</json:string><json:string>mitochondrial</json:string><json:string>plasmid transcript</json:string><json:string>dgmp</json:string><json:string>intron</json:string><json:string>kuiper</json:string><json:string>kennell</json:string><json:string>neurospora</json:string><json:string>rnaase</json:string><json:string>cdna product</json:string><json:string>phosphatase</json:string><json:string>alkaline phosphatase</json:string><json:string>clone</json:string><json:string>digestion</json:string><json:string>template</json:string><json:string>kinase</json:string><json:string>akins</json:string><json:string>oligonucleotides</json:string><json:string>nsil</json:string><json:string>transcription</json:string><json:string>pbluescript</json:string><json:string>biol</json:string><json:string>eickbush</json:string><json:string>polyacrylamide</json:string><json:string>ligated</json:string><json:string>polynucleotide kinase</json:string><json:string>mitochondrion</json:string><json:string>polynucleotide</json:string><json:string>penultimate</json:string><json:string>unpublished data</json:string><json:string>internal site</json:string><json:string>nucleotide</json:string><json:string>replication</json:string><json:string>initiation reaction</json:string><json:string>experimental procedures</json:string><json:string>initiation site</json:string><json:string>strand synthesis</json:string><json:string>transcript</json:string><json:string>initiation</json:string><json:string>cdna primers</json:string><json:string>molecular weight markers</json:string><json:string>prime cdna synthesis</json:string><json:string>reaction medium</json:string><json:string>england biolabs</json:string><json:string>sau3al fragments</json:string><json:string>standard reaction medium</json:string><json:string>mauriceville plasmid enzyme</json:string><json:string>urea</json:string><json:string>protease</json:string><json:string>same site</json:string><json:string>mauriceville plasmid transcript</json:string><json:string>higher concentrations</json:string><json:string>cdna products</json:string><json:string>nucleic acids</json:string><json:string>cdna copy</json:string><json:string>nontemplated addition</json:string><json:string>cdna band</json:string><json:string>evolutionary significance</json:string><json:string>acid primer</json:string><json:string>internal initiation</json:string></teeft></keywords><author><json:item><name>He Wang</name><affiliations><json:string>Department of Molecular Genetics The Ohio State University Columbus, Ohio 43210 USA</json:string><json:string>Department of Biochemistry The Ohio State University Columbus, Ohio 43210 USA</json:string><json:string>Department of Medical Biochemistry The Ohio State University Columbus, Ohio 43210 USA</json:string><json:string>the Biotechnology Center The Ohio State University Columbus, Ohio 43210 USA</json:string></affiliations></json:item><json:item><name>Alan M. Lambowitz</name><affiliations><json:string>Department of Molecular Genetics The Ohio State University Columbus, Ohio 43210 USA</json:string><json:string>Department of Biochemistry The Ohio State University Columbus, Ohio 43210 USA</json:string><json:string>Department of Medical Biochemistry The Ohio State University Columbus, Ohio 43210 USA</json:string><json:string>the Biotechnology Center The Ohio State University Columbus, Ohio 43210 USA</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-RHKD81FR-N</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: We show that the reverse transcriptase (RT) encoded by the Mauriceville mitochondrial plasmid of Neurospora closely resembles viral RNA-dependent RNA polymerases in initiating cDNA synthesis opposite the penultimate C residue of a 3′ tRNA-like structure and has the unprecedented ability for a DNA polymerase to initiate DNA synthesis at a specific site in a natural template without a primer. The Mauriceville plasmid enzyme can also use DNA or RNA primers in a manner suggesting how a primitive RT could have evolved from an RNA-dependent RNA polymerase into retroviral and other types of RTs. The characteristics of the Mauriceville plasmid RT suggest that it may be related to the progenitor of present-day RTs and DNA polymerases.</abstract><qualityIndicators><score>8.392</score><pdfWordCount>8883</pdfWordCount><pdfCharCount>55476</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>11</pdfPageCount><pdfPageSize>612.28 x 821 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>116</abstractWordCount><abstractCharCount>745</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor</title><pmid><json:string>7505202</json:string></pmid><pii><json:string>0092-8674(93)90317-J</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. Sci. 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(1984)</json:string><json:string>Kennell et al</json:string><json:string>Panet et al., 1975</json:string><json:string>Maizels and Weiner (1993)</json:string><json:string>Weiner and Maizets (1987)</json:string><json:string>Rao et al., 1989</json:string><json:string>Wang et al., 1992</json:string><json:string>Grosshans and Cech, 1991</json:string><json:string>Blackburn, 1991</json:string><json:string>Darnell and Doolittle, 1988</json:string><json:string>Eickbush, 1994</json:string><json:string>Kuiper et al., 1990</json:string><json:string>Inouye and Inouye, 1991</json:string><json:string>Delarue et al., 1990</json:string><json:string>Akins et al., 1989</json:string><json:string>Deininger, 1989</json:string><json:string>Ferat and Michel, 1993</json:string><json:string>Varmus and Brown (1989)</json:string><json:string>Gilbert, 1988</json:string><json:string>Davis and de Serres, 1970</json:string><json:string>Tessier et al., 1988</json:string><json:string>Akins et al., 1988</json:string><json:string>Lambowitz, 1979</json:string><json:string>Kornberg and Baker, 1992</json:string><json:string>Xiong and Eickbush, 1990</json:string><json:string>Akins et al. (1988)</json:string><json:string>Clark, 1988</json:string><json:string>Saville and Collins, 1990</json:string><json:string>Kennell et al.</json:string><json:string>Mans et al. (1991)</json:string><json:string>Wang and Lambowitz, 1993</json:string><json:string>Akins et al., 1986, 1989</json:string><json:string>Blumenthal and Carmichael, 1979</json:string><json:string>Wang and Seeger, 1992</json:string><json:string>Temin, 1989</json:string><json:string>Nargang et al., 1984</json:string><json:string>Miller et al., 1988</json:string><json:string>Kohlstaedt et al., 1992</json:string><json:string>Wintersberger and Wintersberger, 1987</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-RHKD81FR-N</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - cell biology</json:string><json:string>2 - biochemistry & molecular biology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - developmental biology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - 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The Mauriceville plasmid enzyme can also use DNA or RNA primers in a manner suggesting how a primitive RT could have evolved from an RNA-dependent RNA polymerase into retroviral and other types of RTs. The characteristics of the Mauriceville plasmid RT suggest that it may be related to the progenitor of present-day RTs and DNA polymerases.</p></abstract></profileDesc><revisionDesc><change when="1993-09-21">Modified</change><change when="1993">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RHKD81FR-N/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>CELL</jid><aid>9390317J</aid><ce:pii>0092-8674(93)90317-J</ce:pii><ce:doi>10.1016/0092-8674(93)90317-J</ce:doi><ce:copyright type="unknown" year="1993"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Article</ce:textfn></ce:dochead><ce:title>The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor</ce:title><ce:author-group><ce:author><ce:given-name>He</ce:given-name><ce:surname>Wang</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF4"><ce:sup>4</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Alan M.</ce:given-name><ce:surname>Lambowitz</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF4"><ce:sup>4</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Department of Molecular Genetics The Ohio State University Columbus, Ohio 43210 USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Department of Biochemistry The Ohio State University Columbus, Ohio 43210 USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Department of Medical Biochemistry The Ohio State University Columbus, Ohio 43210 USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF4"><ce:label>d</ce:label><ce:textfn>the Biotechnology Center The Ohio State University Columbus, Ohio 43210 USA</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="17" month="8" year="1993"></ce:date-received><ce:date-revised day="21" month="9" year="1993"></ce:date-revised><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>We show that the reverse transcriptase (RT) encoded by the Mauriceville mitochondrial plasmid of Neurospora closely resembles viral RNA-dependent RNA polymerases in initiating cDNA synthesis opposite the penultimate C residue of a 3′ tRNA-like structure and has the unprecedented ability for a DNA polymerase to initiate DNA synthesis at a specific site in a natural template without a primer. The Mauriceville plasmid enzyme can also use DNA or RNA primers in a manner suggesting how a primitive RT could have evolved from an RNA-dependent RNA polymerase into retroviral and other types of RTs. The characteristics of the Mauriceville plasmid RT suggest that it may be related to the progenitor of present-day RTs and DNA polymerases.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor</title></titleInfo><name type="personal"><namePart type="given">He</namePart><namePart type="family">Wang</namePart><affiliation>Department of Molecular Genetics The Ohio State University Columbus, Ohio 43210 USA</affiliation><affiliation>Department of Biochemistry The Ohio State University Columbus, Ohio 43210 USA</affiliation><affiliation>Department of Medical Biochemistry The Ohio State University Columbus, Ohio 43210 USA</affiliation><affiliation>the Biotechnology Center The Ohio State University Columbus, Ohio 43210 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Alan M.</namePart><namePart type="family">Lambowitz</namePart><affiliation>Department of Molecular Genetics The Ohio State University Columbus, Ohio 43210 USA</affiliation><affiliation>Department of Biochemistry The Ohio State University Columbus, Ohio 43210 USA</affiliation><affiliation>Department of Medical Biochemistry The Ohio State University Columbus, Ohio 43210 USA</affiliation><affiliation>the Biotechnology Center The Ohio State University Columbus, Ohio 43210 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued><dateModified encoding="w3cdtf">1993-09-21</dateModified><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: We show that the reverse transcriptase (RT) encoded by the Mauriceville mitochondrial plasmid of Neurospora closely resembles viral RNA-dependent RNA polymerases in initiating cDNA synthesis opposite the penultimate C residue of a 3′ tRNA-like structure and has the unprecedented ability for a DNA polymerase to initiate DNA synthesis at a specific site in a natural template without a primer. The Mauriceville plasmid enzyme can also use DNA or RNA primers in a manner suggesting how a primitive RT could have evolved from an RNA-dependent RNA polymerase into retroviral and other types of RTs. The characteristics of the Mauriceville plasmid RT suggest that it may be related to the progenitor of present-day RTs and DNA polymerases.</abstract><note type="content">Section title: Article</note><relatedItem type="host"><titleInfo><title>Cell</title></titleInfo><titleInfo type="abbreviated"><title>CELL</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued></originInfo><identifier type="ISSN">0092-8674</identifier><identifier type="PII">S0092-8674(00)X0364-9</identifier><part><date>1993</date><detail type="volume"><number>75</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1039</start><end>1249</end></extent><extent unit="pages"><start>1071</start><end>1081</end></extent></part></relatedItem><identifier type="istex">CCCA4A5509EB1DF412D478F49D5CAFA5EA86708C</identifier><identifier type="ark">ark:/67375/6H6-RHKD81FR-N</identifier><identifier type="DOI">10.1016/0092-8674(93)90317-J</identifier><identifier type="PII">0092-8674(93)90317-J</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RHKD81FR-N/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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