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Genetic Analysis of the Monopartite Tomato Yellow Leaf Curl Geminivirus: Roles of V1, V2, and C2 ORFs in Viral Pathogenesis

Identifieur interne : 001E57 ( Istex/Curation ); précédent : 001E56; suivant : 001E58

Genetic Analysis of the Monopartite Tomato Yellow Leaf Curl Geminivirus: Roles of V1, V2, and C2 ORFs in Viral Pathogenesis

Auteurs : Leila Wartig [France] ; Ahmed Kheyr-Pour [France] ; Emanuela Noris [France] ; Françoise De Kouchkovsky [France] ; Françoise Jouanneau [France] ; Bruno Gronenborn [France] ; Isabelle Jupin [France]

Source :

Virology [ 0042-6822 ] ; 1997.

RBID : ISTEX:5FE9B029172FB7971658DBB5C1CECAD1448A3F47

English descriptors

Teeft :
- Alkaline treatment, Amino acids, Benthamiana, Benthamiana leaf discs, Benthamiana plants, Bipartite, Bipartite geminiviruses, Bisaro, Capsid protein, Coat protein, Dsdna, Geminivirus, Geminiviruses, Gene function, Gene product, Gene products, Genetic analysis, Genome, Infectivity, Inoculated, Inoculated plants, Jupin, Leaf discs, Leaf discs inoculated, Maize streak virus, Monopartite, Monopartite geminiviruses, Mosaic virus, Mutant, Mutation, Mutational analysis, Nucleic acids, Orfs, Other hand, Other subgroup, Plants inoculated, Protoplast, Replication, Rigden, Southern blot, Ssdna, Ssdna forms, Subgroup, Systemic movement, Systemic spread, Tomato, Tomato leaf, Tomato plants, Tomato protoplasts, Tylcv, Tylcv mutants, Tylcv pathogenicity, Vira, Viral, Virol, Virology, Virology determinants, Virus particles, Western blots.

Abstract

Abstract: Tomato yellow leaf curl virus (TYLCV) is a whitefly-transmitted geminivirus with a monopartite genome. We have investigated the functions of the V1, V2, and C2 ORFs by mutational analysis. We analyzed the ability of TYLCV mutants containing disrupted ORFs V1, V2, or C2 to replicate, spread, and cause symptoms inNicotiana benthamianaand tomato plants. All the mutants retained the capability of autonomous replication in protoplast-derived cells of tomato and leaf discs ofN. benthamiana,although both V1 and V2 gene products appeared to play a role in the accumulation of viral single-stranded DNA. In contrast, none of the mutants was able to systemically infect tomato plants, demonstrating that the V1, V2, and C2 gene products are all required for a successful infection process in this host. The effect of the mutation in ORF C2 appeared to be host-specific, sinceN. benthamianaplants were systemically infected, although symptom development was attenuated.

Url:

https://api.istex.fr/ark:/67375/6H6-GC8XX063-R/fulltext.pdf

DOI: 10.1006/viro.1996.8406

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<term>Benthamiana plants</term>
<term>Bipartite</term>
<term>Bipartite geminiviruses</term>
<term>Bisaro</term>
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<term>Geminiviruses</term>
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<term>Gene products</term>
<term>Genetic analysis</term>
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<term>Infectivity</term>
<term>Inoculated</term>
<term>Inoculated plants</term>
<term>Jupin</term>
<term>Leaf discs</term>
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<term>Monopartite geminiviruses</term>
<term>Mosaic virus</term>
<term>Mutant</term>
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<term>Mutational analysis</term>
<term>Nucleic acids</term>
<term>Orfs</term>
<term>Other hand</term>
<term>Other subgroup</term>
<term>Plants inoculated</term>
<term>Protoplast</term>
<term>Replication</term>
<term>Rigden</term>
<term>Southern blot</term>
<term>Ssdna</term>
<term>Ssdna forms</term>
<term>Subgroup</term>
<term>Systemic movement</term>
<term>Systemic spread</term>
<term>Tomato</term>
<term>Tomato leaf</term>
<term>Tomato plants</term>
<term>Tomato protoplasts</term>
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<term>Tylcv mutants</term>
<term>Tylcv pathogenicity</term>
<term>Vira</term>
<term>Viral</term>
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<front><div type="abstract" xml:lang="en">Abstract: Tomato yellow leaf curl virus (TYLCV) is a whitefly-transmitted geminivirus with a monopartite genome. We have investigated the functions of the V1, V2, and C2 ORFs by mutational analysis. We analyzed the ability of TYLCV mutants containing disrupted ORFs V1, V2, or C2 to replicate, spread, and cause symptoms inNicotiana benthamianaand tomato plants. All the mutants retained the capability of autonomous replication in protoplast-derived cells of tomato and leaf discs ofN. benthamiana,although both V1 and V2 gene products appeared to play a role in the accumulation of viral single-stranded DNA. In contrast, none of the mutants was able to systemically infect tomato plants, demonstrating that the V1, V2, and C2 gene products are all required for a successful infection process in this host. The effect of the mutation in ORF C2 appeared to be host-specific, sinceN. benthamianaplants were systemically infected, although symptom development was attenuated.</div>
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Genetic Analysis of the Monopartite Tomato Yellow Leaf Curl Geminivirus: Roles of V1, V2, and C2 ORFs in Viral Pathogenesis

Genetic Analysis of the Monopartite Tomato Yellow Leaf Curl Geminivirus: Roles of V1, V2, and C2 ORFs in Viral Pathogenesis

Source :

English descriptors

Abstract

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