Genetic Analysis of the Monopartite Tomato Yellow Leaf Curl Geminivirus: Roles of V1, V2, and C2 ORFs in Viral Pathogenesis
Identifieur interne : 001E57 ( Istex/Corpus ); précédent : 001E56; suivant : 001E58Genetic Analysis of the Monopartite Tomato Yellow Leaf Curl Geminivirus: Roles of V1, V2, and C2 ORFs in Viral Pathogenesis
Auteurs : Leila Wartig ; Ahmed Kheyr-Pour ; Emanuela Noris ; Françoise De Kouchkovsky ; Françoise Jouanneau ; Bruno Gronenborn ; Isabelle JupinSource :
- Virology [ 0042-6822 ] ; 1997.
English descriptors
- Teeft :
- Alkaline treatment, Amino acids, Benthamiana, Benthamiana leaf discs, Benthamiana plants, Bipartite, Bipartite geminiviruses, Bisaro, Capsid protein, Coat protein, Dsdna, Geminivirus, Geminiviruses, Gene function, Gene product, Gene products, Genetic analysis, Genome, Infectivity, Inoculated, Inoculated plants, Jupin, Leaf discs, Leaf discs inoculated, Maize streak virus, Monopartite, Monopartite geminiviruses, Mosaic virus, Mutant, Mutation, Mutational analysis, Nucleic acids, Orfs, Other hand, Other subgroup, Plants inoculated, Protoplast, Replication, Rigden, Southern blot, Ssdna, Ssdna forms, Subgroup, Systemic movement, Systemic spread, Tomato, Tomato leaf, Tomato plants, Tomato protoplasts, Tylcv, Tylcv mutants, Tylcv pathogenicity, Vira, Viral, Virol, Virology, Virology determinants, Virus particles, Western blots.
Abstract
Abstract: Tomato yellow leaf curl virus (TYLCV) is a whitefly-transmitted geminivirus with a monopartite genome. We have investigated the functions of the V1, V2, and C2 ORFs by mutational analysis. We analyzed the ability of TYLCV mutants containing disrupted ORFs V1, V2, or C2 to replicate, spread, and cause symptoms inNicotiana benthamianaand tomato plants. All the mutants retained the capability of autonomous replication in protoplast-derived cells of tomato and leaf discs ofN. benthamiana,although both V1 and V2 gene products appeared to play a role in the accumulation of viral single-stranded DNA. In contrast, none of the mutants was able to systemically infect tomato plants, demonstrating that the V1, V2, and C2 gene products are all required for a successful infection process in this host. The effect of the mutation in ORF C2 appeared to be host-specific, sinceN. benthamianaplants were systemically infected, although symptom development was attenuated.
Url:
DOI: 10.1006/viro.1996.8406
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ISTEX:5FE9B029172FB7971658DBB5C1CECAD1448A3F47Le document en format XML
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Curl Geminivirus: Roles of V1, V2, and C2 ORFs in Viral Pathogenesis</title><author><name sortKey="Wartig, Leila" sort="Wartig, Leila" uniqKey="Wartig L" first="Leila" last="Wartig">Leila Wartig</name><affiliation><mods:affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</mods:affiliation></affiliation></author><author><name sortKey="Kheyr Pour, Ahmed" sort="Kheyr Pour, Ahmed" uniqKey="Kheyr Pour A" first="Ahmed" last="Kheyr-Pour">Ahmed Kheyr-Pour</name><affiliation><mods:affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</mods:affiliation></affiliation></author><author><name sortKey="Noris, Emanuela" sort="Noris, Emanuela" uniqKey="Noris E" first="Emanuela" last="Noris">Emanuela Noris</name><affiliation><mods:affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</mods:affiliation></affiliation></author><author><name sortKey="De Kouchkovsky, Francoise" sort="De Kouchkovsky, Francoise" uniqKey="De Kouchkovsky F" first="Françoise" last="De Kouchkovsky">Françoise De Kouchkovsky</name><affiliation><mods:affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</mods:affiliation></affiliation></author><author><name sortKey="Jouanneau, Francoise" sort="Jouanneau, Francoise" uniqKey="Jouanneau F" first="Françoise" last="Jouanneau">Françoise Jouanneau</name><affiliation><mods:affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</mods:affiliation></affiliation></author><author><name sortKey="Gronenborn, Bruno" sort="Gronenborn, Bruno" uniqKey="Gronenborn B" first="Bruno" last="Gronenborn">Bruno Gronenborn</name><affiliation><mods:affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</mods:affiliation></affiliation></author><author><name sortKey="Jupin, Isabelle" sort="Jupin, Isabelle" uniqKey="Jupin I" first="Isabelle" last="Jupin">Isabelle Jupin</name><affiliation><mods:affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997">1997</date><biblScope unit="volume">228</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="132">132</biblScope><biblScope unit="page" to="140">140</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Alkaline treatment</term><term>Amino acids</term><term>Benthamiana</term><term>Benthamiana leaf discs</term><term>Benthamiana plants</term><term>Bipartite</term><term>Bipartite geminiviruses</term><term>Bisaro</term><term>Capsid protein</term><term>Coat protein</term><term>Dsdna</term><term>Geminivirus</term><term>Geminiviruses</term><term>Gene function</term><term>Gene product</term><term>Gene products</term><term>Genetic analysis</term><term>Genome</term><term>Infectivity</term><term>Inoculated</term><term>Inoculated plants</term><term>Jupin</term><term>Leaf discs</term><term>Leaf discs inoculated</term><term>Maize streak virus</term><term>Monopartite</term><term>Monopartite geminiviruses</term><term>Mosaic virus</term><term>Mutant</term><term>Mutation</term><term>Mutational analysis</term><term>Nucleic acids</term><term>Orfs</term><term>Other hand</term><term>Other subgroup</term><term>Plants inoculated</term><term>Protoplast</term><term>Replication</term><term>Rigden</term><term>Southern blot</term><term>Ssdna</term><term>Ssdna forms</term><term>Subgroup</term><term>Systemic movement</term><term>Systemic spread</term><term>Tomato</term><term>Tomato leaf</term><term>Tomato plants</term><term>Tomato protoplasts</term><term>Tylcv</term><term>Tylcv mutants</term><term>Tylcv pathogenicity</term><term>Vira</term><term>Viral</term><term>Virol</term><term>Virology</term><term>Virology determinants</term><term>Virus particles</term><term>Western blots</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Tomato yellow leaf curl virus (TYLCV) is a whitefly-transmitted geminivirus with a monopartite genome. We have investigated the functions of the V1, V2, and C2 ORFs by mutational analysis. We analyzed the ability of TYLCV mutants containing disrupted ORFs V1, V2, or C2 to replicate, spread, and cause symptoms inNicotiana benthamianaand tomato plants. All the mutants retained the capability of autonomous replication in protoplast-derived cells of tomato and leaf discs ofN. benthamiana,although both V1 and V2 gene products appeared to play a role in the accumulation of viral single-stranded DNA. In contrast, none of the mutants was able to systemically infect tomato plants, demonstrating that the V1, V2, and C2 gene products are all required for a successful infection process in this host. The effect of the mutation in ORF C2 appeared to be host-specific, sinceN. benthamianaplants were systemically infected, although symptom development was attenuated.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>mutant</json:string><json:string>tylcv</json:string><json:string>viral</json:string><json:string>benthamiana</json:string><json:string>virology</json:string><json:string>geminivirus</json:string><json:string>inoculated</json:string><json:string>ssdna</json:string><json:string>geminiviruses</json:string><json:string>mutation</json:string><json:string>orfs</json:string><json:string>genome</json:string><json:string>dsdna</json:string><json:string>subgroup</json:string><json:string>infectivity</json:string><json:string>monopartite</json:string><json:string>bisaro</json:string><json:string>protoplast</json:string><json:string>jupin</json:string><json:string>leaf discs</json:string><json:string>vira</json:string><json:string>tylcv mutants</json:string><json:string>rigden</json:string><json:string>replication</json:string><json:string>bipartite</json:string><json:string>virol</json:string><json:string>benthamiana leaf discs</json:string><json:string>tomato plants</json:string><json:string>benthamiana plants</json:string><json:string>tomato</json:string><json:string>coat protein</json:string><json:string>gene products</json:string><json:string>genetic analysis</json:string><json:string>gene product</json:string><json:string>inoculated plants</json:string><json:string>mutational analysis</json:string><json:string>tomato leaf</json:string><json:string>nucleic acids</json:string><json:string>plants inoculated</json:string><json:string>tylcv pathogenicity</json:string><json:string>amino acids</json:string><json:string>mosaic virus</json:string><json:string>monopartite geminiviruses</json:string><json:string>bipartite geminiviruses</json:string><json:string>western blots</json:string><json:string>leaf discs inoculated</json:string><json:string>systemic movement</json:string><json:string>other subgroup</json:string><json:string>ssdna forms</json:string><json:string>tomato protoplasts</json:string><json:string>southern blot</json:string><json:string>virus particles</json:string><json:string>gene function</json:string><json:string>other hand</json:string><json:string>capsid protein</json:string><json:string>alkaline treatment</json:string><json:string>virology determinants</json:string><json:string>maize streak virus</json:string><json:string>systemic spread</json:string></teeft></keywords><author><json:item><name>Leila Wartig</name><affiliations><json:string>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</json:string></affiliations></json:item><json:item><name>Ahmed Kheyr-Pour</name><affiliations><json:string>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</json:string></affiliations></json:item><json:item><name>Emanuela Noris</name><affiliations><json:string>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</json:string></affiliations></json:item><json:item><name>Françoise De Kouchkovsky</name><affiliations><json:string>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</json:string></affiliations></json:item><json:item><name>Françoise Jouanneau</name><affiliations><json:string>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</json:string></affiliations></json:item><json:item><name>Bruno Gronenborn</name><affiliations><json:string>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</json:string></affiliations></json:item><json:item><name>Isabelle Jupin</name><affiliations><json:string>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-GC8XX063-R</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Tomato yellow leaf curl virus (TYLCV) is a whitefly-transmitted geminivirus with a monopartite genome. We have investigated the functions of the V1, V2, and C2 ORFs by mutational analysis. We analyzed the ability of TYLCV mutants containing disrupted ORFs V1, V2, or C2 to replicate, spread, and cause symptoms inNicotiana benthamianaand tomato plants. All the mutants retained the capability of autonomous replication in protoplast-derived cells of tomato and leaf discs ofN. benthamiana,although both V1 and V2 gene products appeared to play a role in the accumulation of viral single-stranded DNA. In contrast, none of the mutants was able to systemically infect tomato plants, demonstrating that the V1, V2, and C2 gene products are all required for a successful infection process in this host. The effect of the mutation in ORF C2 appeared to be host-specific, sinceN. benthamianaplants were systemically infected, although symptom development was attenuated.</abstract><qualityIndicators><score>8.752</score><pdfWordCount>6307</pdfWordCount><pdfCharCount>38332</pdfCharCount><pdfVersion>1.1</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>532 x 711 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>146</abstractWordCount><abstractCharCount>974</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Genetic Analysis of the Monopartite Tomato Yellow Leaf Curl Geminivirus: Roles of V1, V2, and C2 ORFs in Viral Pathogenesis</title><pmid><json:string>9123819</json:string></pmid><pii><json:string>S0042-6822(96)98406-8</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>1997</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0125-0</json:string></pii><volume>228</volume><issue>2</issue><pages><first>132</first><last>140</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1997</json:string><json:string>01-16-97</json:string></date><geogName></geogName><orgName><json:string>National Research Council of Italy</json:string></orgName><orgName_funder><json:string>National Research Council of Italy</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>BglII</json:string><json:string>R. Milne</json:string><json:string>E. Luisoni</json:string><json:string>P. Accotto</json:string><json:string>ClaI</json:string><json:string>The</json:string><json:string>L. Haenni</json:string><json:string>J. Gen</json:string><json:string>A. J. Gen</json:string><json:string>Mac Farlane</json:string><json:string>La Rosa</json:string></persName><placeName><json:string>Jordan</json:string><json:string>America</json:string><json:string>York</json:string><json:string>Italy</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Jupin et al., 1994</json:string><json:string>Kheyr-Pour et al., 1991</json:string><json:string>Azzam et al., 1994</json:string><json:string>Francki et al., 1991</json:string><json:string>Haley et al., 1992</json:string><json:string>Hamilton et al., 1983</json:string><json:string>Etessami et al., 1991</json:string><json:string>Rigden et al., 1993</json:string><json:string>Pooma and Petty, 1996</json:string><json:string>Boulton et al., 1993</json:string><json:string>Grimsley et al., 1986</json:string><json:string>Jupin et al. (1994)</json:string><json:string>Pooma et al., 1996</json:string><json:string>Hormuzdi and Bisaro, 1993</json:string><json:string>reviewed in Maule, 1991</json:string><json:string>Noris et al., 1994</json:string><json:string>ArguelloAstorga et al., 1994</json:string><json:string>Horsch et al. (1984)</json:string><json:string>Lazarowitz, 1992</json:string><json:string>Wu and Wang (1984)</json:string><json:string>Etessami et al., 1989</json:string><json:string>Boulton et al., 1989, 1993</json:string><json:string>Kunkel, 1985</json:string><json:string>Dickinson et al., 1996</json:string><json:string>Padidam et al., 1996</json:string><json:string>Elmer et al., 1988</json:string><json:string>Ausubel et al., 1987</json:string><json:string>Sunter and Bisaro, 1991, 1992</json:string><json:string>Noueiry et al., 1994</json:string><json:string>Sunter et al., 1990</json:string><json:string>Bevan, 1984</json:string><json:string>Luisoni et al., 1992</json:string><json:string>Stanley, 1983</json:string><json:string>Stanley et al., 1992</json:string><json:string>Czosnek et al., 1990</json:string><json:string>Bendhamane et al., 1993</json:string><json:string>Cohen and Nitzany, 1966</json:string><json:string>Kornberg and Baker, 1992</json:string><json:string>for reviews see Stanley, 1991</json:string><json:string>Kheyr-Pour et al. (1991)</json:string><json:string>Boulton et al., 1989</json:string><json:string>Sambrook et al., 1989</json:string><json:string>Hormuzdi and Bisaro, 1995</json:string><json:string>Briddon et al., 1990</json:string><json:string>Noris et al., 1996</json:string><json:string>Navot et al., 1991</json:string><json:string>Rigden et al., 1994</json:string><json:string>Czosnek et al., 1988</json:string><json:string>Briddon et al., 1989</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-GC8XX063-R</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - 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xml:lang="en">Genetic Analysis of the Monopartite Tomato Yellow Leaf Curl Geminivirus: Roles of V1, V2, and C2 ORFs in Viral Pathogenesis</title><author xml:id="author-0000"><persName><forename type="first">Leila</forename><surname>Wartig</surname></persName><note type="biography">These authors contributed equally to this work.</note><affiliation>These authors contributed equally to this work.</affiliation><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Ahmed</forename><surname>Kheyr-Pour</surname></persName><note type="biography">These authors contributed equally to this work.</note><affiliation>These authors contributed equally to this work.</affiliation><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Emanuela</forename><surname>Noris</surname></persName><note type="biography">Present address: Istituto di Fitovirologia Applicata, CNR, Strada delle Cacce 73, 10135 Torino, Italy.</note><affiliation>Present address: Istituto di Fitovirologia Applicata, CNR, Strada delle Cacce 73, 10135 Torino, Italy.</affiliation><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Françoise</forename><surname>De Kouchkovsky</surname></persName><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Françoise</forename><surname>Jouanneau</surname></persName><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Bruno</forename><surname>Gronenborn</surname></persName><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation></author><author xml:id="author-0006"><persName><forename type="first">Isabelle</forename><surname>Jupin</surname></persName><affiliation>To whom correspondence and reprint requests should be addressed at present address: Institut Jacques Monod, CNRS-Université Paris 7, 2 place Jussieu, 75251 Paris Cedex 05, France.</affiliation><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation></author><idno type="istex">5FE9B029172FB7971658DBB5C1CECAD1448A3F47</idno><idno type="ark">ark:/67375/6H6-GC8XX063-R</idno><idno type="DOI">10.1006/viro.1996.8406</idno><idno type="PII">S0042-6822(96)98406-8</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0125-0</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997"></date><biblScope unit="volume">228</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="132">132</biblScope><biblScope unit="page" to="140">140</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1997</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Tomato yellow leaf curl virus (TYLCV) is a whitefly-transmitted geminivirus with a monopartite genome. We have investigated the functions of the V1, V2, and C2 ORFs by mutational analysis. We analyzed the ability of TYLCV mutants containing disrupted ORFs V1, V2, or C2 to replicate, spread, and cause symptoms inNicotiana benthamianaand tomato plants. All the mutants retained the capability of autonomous replication in protoplast-derived cells of tomato and leaf discs ofN. benthamiana,although both V1 and V2 gene products appeared to play a role in the accumulation of viral single-stranded DNA. In contrast, none of the mutants was able to systemically infect tomato plants, demonstrating that the V1, V2, and C2 gene products are all required for a successful infection process in this host. The effect of the mutation in ORF C2 appeared to be host-specific, sinceN. benthamianaplants were systemically infected, although symptom development was attenuated.</p></abstract></profileDesc><revisionDesc><change when="1996-10-04">Modified</change><change when="1997">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-GC8XX063-R/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YVIRO</jid><aid>98406</aid><ce:pii>S0042-6822(96)98406-8</ce:pii><ce:doi>10.1006/viro.1996.8406</ce:doi><ce:copyright type="full-transfer" year="1997">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Genetic Analysis of the Monopartite Tomato Yellow Leaf Curl Geminivirus: Roles of V1, V2, and C2 ORFs in Viral Pathogenesis</ce:title><ce:author-group><ce:author><ce:given-name>Leila</ce:given-name><ce:surname>Wartig</ce:surname><ce:cross-ref refid="VY968406FN1">1<ce:footnote id="VY968406FN1"><ce:label>1</ce:label><ce:note-para>These authors contributed equally to this work.</ce:note-para></ce:footnote></ce:cross-ref></ce:author><ce:author><ce:given-name>Ahmed</ce:given-name><ce:surname>Kheyr-Pour</ce:surname><ce:cross-ref refid="VY968406FN1">1</ce:cross-ref></ce:author><ce:author><ce:given-name>Emanuela</ce:given-name><ce:surname>Noris</ce:surname><ce:cross-ref refid="VY968406FN2">2<ce:footnote id="VY968406FN2"><ce:label>2</ce:label><ce:note-para>Present address: Istituto di Fitovirologia Applicata, CNR, Strada delle Cacce 73, 10135 Torino, Italy.</ce:note-para></ce:footnote></ce:cross-ref></ce:author><ce:author><ce:given-name>Françoise</ce:given-name><ce:surname>De Kouchkovsky</ce:surname></ce:author><ce:author><ce:given-name>Françoise</ce:given-name><ce:surname>Jouanneau</ce:surname></ce:author><ce:author><ce:given-name>Bruno</ce:given-name><ce:surname>Gronenborn</ce:surname></ce:author><ce:author><ce:given-name>Isabelle</ce:given-name><ce:surname>Jupin</ce:surname><ce:cross-ref refid="VY968406FN3">3<ce:footnote id="VY968406FN3"><ce:label>3</ce:label><ce:note-para>To whom correspondence and reprint requests should be addressed at present address: Institut Jacques Monod, CNRS-Université Paris 7, 2 place Jussieu, 75251 Paris Cedex 05, France.</ce:note-para></ce:footnote></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="27" month="8" year="1996"></ce:date-received><ce:date-revised day="4" month="10" year="1996"></ce:date-revised><ce:date-accepted day="12" month="12" year="1996"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Tomato yellow leaf curl virus (TYLCV) is a whitefly-transmitted geminivirus with a monopartite genome. We have investigated the functions of the V1, V2, and C2 ORFs by mutational analysis. We analyzed the ability of TYLCV mutants containing disrupted ORFs V1, V2, or C2 to replicate, spread, and cause symptoms in<ce:italic>Nicotiana benthamiana</ce:italic>and tomato plants. All the mutants retained the capability of autonomous replication in protoplast-derived cells of tomato and leaf discs of<ce:italic>N. benthamiana,</ce:italic>although both V1 and V2 gene products appeared to play a role in the accumulation of viral single-stranded DNA. In contrast, none of the mutants was able to systemically infect tomato plants, demonstrating that the V1, V2, and C2 gene products are all required for a successful infection process in this host. The effect of the mutation in ORF C2 appeared to be host-specific, since<ce:italic>N. benthamiana</ce:italic>plants were systemically infected, although symptom development was attenuated.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Genetic Analysis of the Monopartite Tomato Yellow Leaf Curl Geminivirus: Roles of V1, V2, and C2 ORFs in Viral Pathogenesis</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Genetic Analysis of the Monopartite Tomato Yellow Leaf Curl Geminivirus: Roles of V1, V2, and C2 ORFs in Viral Pathogenesis</title></titleInfo><name type="personal"><namePart type="given">Leila</namePart><namePart type="family">Wartig</namePart><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation><description>These authors contributed equally to this work.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ahmed</namePart><namePart type="family">Kheyr-Pour</namePart><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation><description>These authors contributed equally to this work.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Emanuela</namePart><namePart type="family">Noris</namePart><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation><description>Present address: Istituto di Fitovirologia Applicata, CNR, Strada delle Cacce 73, 10135 Torino, Italy.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Françoise</namePart><namePart type="family">De Kouchkovsky</namePart><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Françoise</namePart><namePart type="family">Jouanneau</namePart><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bruno</namePart><namePart type="family">Gronenborn</namePart><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Isabelle</namePart><namePart type="family">Jupin</namePart><affiliation>Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France</affiliation><description>To whom correspondence and reprint requests should be addressed at present address: Institut Jacques Monod, CNRS-Université Paris 7, 2 place Jussieu, 75251 Paris Cedex 05, France.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1997</dateIssued><dateModified encoding="w3cdtf">1996-10-04</dateModified><copyrightDate encoding="w3cdtf">1997</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Tomato yellow leaf curl virus (TYLCV) is a whitefly-transmitted geminivirus with a monopartite genome. We have investigated the functions of the V1, V2, and C2 ORFs by mutational analysis. We analyzed the ability of TYLCV mutants containing disrupted ORFs V1, V2, or C2 to replicate, spread, and cause symptoms inNicotiana benthamianaand tomato plants. All the mutants retained the capability of autonomous replication in protoplast-derived cells of tomato and leaf discs ofN. benthamiana,although both V1 and V2 gene products appeared to play a role in the accumulation of viral single-stranded DNA. In contrast, none of the mutants was able to systemically infect tomato plants, demonstrating that the V1, V2, and C2 gene products are all required for a successful infection process in this host. The effect of the mutation in ORF C2 appeared to be host-specific, sinceN. benthamianaplants were systemically infected, although symptom development was attenuated.</abstract><note type="content">Section title: Regular Article</note><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1997</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0125-0</identifier><part><date>1997</date><detail type="volume"><number>228</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>123</start><end>407</end></extent><extent unit="pages"><start>132</start><end>140</end></extent></part></relatedItem><identifier type="istex">5FE9B029172FB7971658DBB5C1CECAD1448A3F47</identifier><identifier type="ark">ark:/67375/6H6-GC8XX063-R</identifier><identifier type="DOI">10.1006/viro.1996.8406</identifier><identifier type="PII">S0042-6822(96)98406-8</identifier><accessCondition type="use and reproduction" contentType="copyright">©1997 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©1997</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-GC8XX063-R/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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