Identification of multiple genes and their expression profiles in four strains of Oreochromis spp. in response to Streptococcus iniae
Identifieur interne : 001B01 ( Istex/Curation ); précédent : 001B00; suivant : 001B02Identification of multiple genes and their expression profiles in four strains of Oreochromis spp. in response to Streptococcus iniae
Auteurs : R. Wang [République populaire de Chine] ; M. Chen [République populaire de Chine] ; C. Li [République populaire de Chine] ; L. Li [République populaire de Chine] ; X. Gan [République populaire de Chine] ; J. Huang [République populaire de Chine] ; A. Lei [République populaire de Chine] ; Z. Xu [République populaire de Chine] ; W. Liang [République populaire de Chine]Source :
- Journal of Fish Biology [ 0022-1112 ] ; 2013-02.
English descriptors
- Teeft :
- Aureus, Aureus strain, Authors journal, British isles, Cdna, Challenge group, Chemokine receptor, Clone, Contig, Contigs, Cxcr4, Danio rerio, Expression levels, Fish biology, Fisheries society, Fishery, Fkcs, Gene expression, Guangxi, Head kidney, Heat shock protein, Immune, Immune response, Infectious salmon anemia virus, Iniae, Leucocyte, Matrix metalloproteinase, Mmp9, Mossambicus, Mrna, Negative correlation, Niloticus, Novel genes, Oreochromis, Oreochromis aureus, Oreochromis mossambicus, Paralichthys olivaceus, Peripheral blood leucocytes, Pgrn, Progranulin, Receptor, Relative expression level, Ribosomal protein, Rna, Salar, Salmo, Salmo salar, Scophthalmus maximus, Seriola quinqueradiata, Singleton, Spleen, Streptococcus, Streptococcus iniae, Subtractive, Suppression subtractive hybridization, Tetraodon nigroviridis, Tilapia, Total rnas, Unnamed protein product, Whole blood.
Abstract
Two subtractive complementary DNA libraries were constructed from Nile tilapia Oreochromis niloticus vaccinated with formalin‐killed Streptococcus iniae cells, and a further two constructed from O. niloticus infected with S. iniae. Of the 68 distinct expressed sequence tag (EST) contigs and singletons, 45 and 13 EST shared high similarities with genes of known and unknown functions, respectively. Ten EST contigs and singletons had no significant similarity to any sequences. Five putative immune‐relevant genes, β2m, α‐ha, mmp9, pgrn and cxcr4, were selected for quantitative reverse‐transcription polymerase chain reaction in four strains of Oreochromis spp.: genetically improved farmed tilapia (O. niloticus), Oreochromis aureus, O. niloticus and O. niloticus×O. aureus, with different disease resistance following infection with S. iniae. pgrn was up‐regulated more significantly in disease‐resistant strains than in the susceptible. α‐ha was markedly down‐regulated, and no significant differences in the expression level of β2m were detected. A negative correlation was observed between the expression of mmp9 and that of cxcr4. The results provide insight into the molecular response of O. niloticus to S. iniae infection.
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DOI: 10.1111/jfb.12004
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Wang</name><affiliation wicri:level="1"><mods:affiliation>Guangxi Institute of Fisheries, Nanning, Guangxi, People's Republic of China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangxi Institute of Fisheries, Nanning, Guangxi</wicri:regionArea></affiliation></author><author><name sortKey="Chen, M" sort="Chen, M" uniqKey="Chen M" first="M." last="Chen">M. Chen</name><affiliation wicri:level="1"><mods:affiliation>Guangxi Institute of Fisheries, Nanning, Guangxi, People's Republic of China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangxi Institute of Fisheries, Nanning, Guangxi</wicri:regionArea></affiliation></author><author><name sortKey="Li, C" sort="Li, C" uniqKey="Li C" first="C." last="Li">C. Li</name><affiliation wicri:level="1"><mods:affiliation>College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, People's Republic of China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Animal Science and Technology, Guangxi University, Nanning, Guangxi</wicri:regionArea></affiliation></author><author><name sortKey="Li, L" sort="Li, L" uniqKey="Li L" first="L." last="Li">L. Li</name><affiliation wicri:level="1"><mods:affiliation>Guangxi Institute of Fisheries, Nanning, Guangxi, People's Republic of China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangxi Institute of Fisheries, Nanning, Guangxi</wicri:regionArea></affiliation></author><author><name sortKey="Gan, X" sort="Gan, X" uniqKey="Gan X" first="X." last="Gan">X. Gan</name><affiliation wicri:level="1"><mods:affiliation>Guangxi Institute of Fisheries, Nanning, Guangxi, People's Republic of China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangxi Institute of Fisheries, Nanning, Guangxi</wicri:regionArea></affiliation></author><author><name sortKey="Huang, J" sort="Huang, J" uniqKey="Huang J" first="J." last="Huang">J. Huang</name><affiliation wicri:level="1"><mods:affiliation>College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, People's Republic of China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Animal Science and Technology, Guangxi University, Nanning, Guangxi</wicri:regionArea></affiliation></author><author><name sortKey="Lei, A" sort="Lei, A" uniqKey="Lei A" first="A." last="Lei">A. 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Liang</name><affiliation wicri:level="1"><mods:affiliation>Guangxi Institute of Fisheries, Nanning, Guangxi, People's Republic of China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangxi Institute of Fisheries, Nanning, Guangxi</wicri:regionArea></affiliation><affiliation><mods:affiliation>E-mail: nnlww@126.com</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Fish Biology</title><title level="j" type="alt">JOURNAL OF FISH BIOLOGY</title><idno type="ISSN">0022-1112</idno><idno type="eISSN">1095-8649</idno><imprint><biblScope unit="vol">82</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="492">492</biblScope><biblScope unit="page" to="504">504</biblScope><biblScope unit="page-count">13</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2013-02">2013-02</date></imprint><idno type="ISSN">0022-1112</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1112</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aureus</term><term>Aureus strain</term><term>Authors journal</term><term>British isles</term><term>Cdna</term><term>Challenge group</term><term>Chemokine receptor</term><term>Clone</term><term>Contig</term><term>Contigs</term><term>Cxcr4</term><term>Danio rerio</term><term>Expression levels</term><term>Fish biology</term><term>Fisheries society</term><term>Fishery</term><term>Fkcs</term><term>Gene expression</term><term>Guangxi</term><term>Head kidney</term><term>Heat shock protein</term><term>Immune</term><term>Immune response</term><term>Infectious salmon anemia virus</term><term>Iniae</term><term>Leucocyte</term><term>Matrix metalloproteinase</term><term>Mmp9</term><term>Mossambicus</term><term>Mrna</term><term>Negative correlation</term><term>Niloticus</term><term>Novel genes</term><term>Oreochromis</term><term>Oreochromis aureus</term><term>Oreochromis mossambicus</term><term>Paralichthys olivaceus</term><term>Peripheral blood leucocytes</term><term>Pgrn</term><term>Progranulin</term><term>Receptor</term><term>Relative expression level</term><term>Ribosomal protein</term><term>Rna</term><term>Salar</term><term>Salmo</term><term>Salmo salar</term><term>Scophthalmus maximus</term><term>Seriola quinqueradiata</term><term>Singleton</term><term>Spleen</term><term>Streptococcus</term><term>Streptococcus iniae</term><term>Subtractive</term><term>Suppression subtractive hybridization</term><term>Tetraodon nigroviridis</term><term>Tilapia</term><term>Total rnas</term><term>Unnamed protein product</term><term>Whole blood</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two subtractive complementary DNA libraries were constructed from Nile tilapia Oreochromis niloticus vaccinated with formalin‐killed Streptococcus iniae cells, and a further two constructed from O. niloticus infected with S. iniae. Of the 68 distinct expressed sequence tag (EST) contigs and singletons, 45 and 13 EST shared high similarities with genes of known and unknown functions, respectively. Ten EST contigs and singletons had no significant similarity to any sequences. Five putative immune‐relevant genes, β2m, α‐ha, mmp9, pgrn and cxcr4, were selected for quantitative reverse‐transcription polymerase chain reaction in four strains of Oreochromis spp.: genetically improved farmed tilapia (O. niloticus), Oreochromis aureus, O. niloticus and O. niloticus×O. aureus, with different disease resistance following infection with S. iniae. pgrn was up‐regulated more significantly in disease‐resistant strains than in the susceptible. α‐ha was markedly down‐regulated, and no significant differences in the expression level of β2m were detected. A negative correlation was observed between the expression of mmp9 and that of cxcr4. The results provide insight into the molecular response of O. niloticus to S. iniae infection.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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