Analysis of imprinting in mice with uniparental duplication of proximal chromosomes 7 and 15 by use of a custom oligonucleotide microarray
Identifieur interne : 001958 ( Istex/Curation ); précédent : 001957; suivant : 001959Analysis of imprinting in mice with uniparental duplication of proximal chromosomes 7 and 15 by use of a custom oligonucleotide microarray
Auteurs : Victoria L. Buettner [États-Unis] ; Jeffrey A. Longmate [États-Unis] ; Michael E. Barish [États-Unis] ; Jeffrey R. Mann [États-Unis] ; Judith Singer-Sam [États-Unis]Source :
- Mammalian Genome [ 0938-8990 ] ; 2004-03-01.
Abstract
Abstract: We have developed an imprinting assay combining the use of mice carrying maternal or paternal duplication of chromosomal regions of interest with custom oligonucleotide microarrays. As a model system, we analyzed RNA from CNS tissue of neonatal mice carrying the reciprocal translocation T(7;15)9H and uniparental duplication of proximal Chr 7 and 15. The duplicated region includes the locus on proximal Chr 7 corresponding to the human Prader-Willi/Angelman Syndrome. The microarray contained 322 oligonucleotides, including probes to detect major genes involved in neural excitability and synaptic transmission, as well as known imprinted genes mapping to proximal Chr 7: Ndn, Snrpn, Mkrn3, Magel2, Peg3, and Ube3a. Imprinting of these genes in neonatal cortex and cerebellum was first confirmed by quantitative RT-PCR. Their inclusion on the microarray thus provided positive controls for evaluating the effect of background on the sensitivity of the assay, and for establishing the minimum level of expression required to detect imprinting. Our analysis extended previous work by revealing bi-allelic expression in CNS tissue of those queried genes mapping to proximal Chr 7 or 15, including the Gabrb3 gene, for which there have been conflicting reports. Microarray analysis also revealed no effect of the maternal or paternal disomy on expression levels of the unlinked genes detected, including those potentially implicated in the Prader-Willi or Angelman Syndrome. In addition, quantitative RT-PCR revealed a gene dosage effect in both cerebellum and cortex for all of the known imprinted genes assayed, except for Ube3a in cerebellum.
Url:
DOI: 10.1007/s00335-003-2322-8
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001958
Links to Exploration step
ISTEX:0BAE691C77D7E54C579F4AD458BB48F1784B0B33Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Analysis of imprinting in mice with uniparental duplication of proximal chromosomes 7 and 15 by use of a custom oligonucleotide microarray</title><author><name sortKey="Buettner, Victoria L" sort="Buettner, Victoria L" uniqKey="Buettner V" first="Victoria L." last="Buettner">Victoria L. Buettner</name><affiliation wicri:level="1"><mods:affiliation>Division of Biology, Beckman Research Institute, City of Hope, CA 91010, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Biology, Beckman Research Institute, City of Hope, CA 91010</wicri:regionArea></affiliation></author><author><name sortKey="Longmate, Jeffrey A" sort="Longmate, Jeffrey A" uniqKey="Longmate J" first="Jeffrey A." last="Longmate">Jeffrey A. Longmate</name><affiliation wicri:level="1"><mods:affiliation>Department of Biostatistics, City of Hope National Medical Center, Duarte, CA 91010, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biostatistics, City of Hope National Medical Center, Duarte, CA 91010</wicri:regionArea></affiliation></author><author><name sortKey="Barish, Michael E" sort="Barish, Michael E" uniqKey="Barish M" first="Michael E." last="Barish">Michael E. Barish</name><affiliation wicri:level="1"><mods:affiliation>Division of Neurosciences, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Neurosciences, Beckman Research Institute, City of Hope, Duarte, CA 91010</wicri:regionArea></affiliation></author><author><name sortKey="Mann, Jeffrey R" sort="Mann, Jeffrey R" uniqKey="Mann J" first="Jeffrey R." last="Mann">Jeffrey R. Mann</name><affiliation wicri:level="1"><mods:affiliation>Division of Biology, Beckman Research Institute, City of Hope, CA 91010, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Biology, Beckman Research Institute, City of Hope, CA 91010</wicri:regionArea></affiliation></author><author><name sortKey="Singer Sam, Judith" sort="Singer Sam, Judith" uniqKey="Singer Sam J" first="Judith" last="Singer-Sam">Judith Singer-Sam</name><affiliation wicri:level="1"><mods:affiliation>Division of Biology, Beckman Research Institute, City of Hope, CA 91010, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Biology, Beckman Research Institute, City of Hope, CA 91010</wicri:regionArea></affiliation><affiliation><mods:affiliation>E-mail: jsam@coh.org</mods:affiliation><wicri:noCountry code="no comma">E-mail: jsam@coh.org</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0BAE691C77D7E54C579F4AD458BB48F1784B0B33</idno><date when="2004" year="2004">2004</date><idno type="doi">10.1007/s00335-003-2322-8</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-JRJHB64N-4/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001958</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001958</idno><idno type="wicri:Area/Istex/Curation">001958</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Analysis of imprinting in mice with uniparental duplication of proximal chromosomes 7 and 15 by use of a custom oligonucleotide microarray</title><author><name sortKey="Buettner, Victoria L" sort="Buettner, Victoria L" uniqKey="Buettner V" first="Victoria L." last="Buettner">Victoria L. Buettner</name><affiliation wicri:level="1"><mods:affiliation>Division of Biology, Beckman Research Institute, City of Hope, CA 91010, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Biology, Beckman Research Institute, City of Hope, CA 91010</wicri:regionArea></affiliation></author><author><name sortKey="Longmate, Jeffrey A" sort="Longmate, Jeffrey A" uniqKey="Longmate J" first="Jeffrey A." last="Longmate">Jeffrey A. Longmate</name><affiliation wicri:level="1"><mods:affiliation>Department of Biostatistics, City of Hope National Medical Center, Duarte, CA 91010, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biostatistics, City of Hope National Medical Center, Duarte, CA 91010</wicri:regionArea></affiliation></author><author><name sortKey="Barish, Michael E" sort="Barish, Michael E" uniqKey="Barish M" first="Michael E." last="Barish">Michael E. Barish</name><affiliation wicri:level="1"><mods:affiliation>Division of Neurosciences, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Neurosciences, Beckman Research Institute, City of Hope, Duarte, CA 91010</wicri:regionArea></affiliation></author><author><name sortKey="Mann, Jeffrey R" sort="Mann, Jeffrey R" uniqKey="Mann J" first="Jeffrey R." last="Mann">Jeffrey R. Mann</name><affiliation wicri:level="1"><mods:affiliation>Division of Biology, Beckman Research Institute, City of Hope, CA 91010, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Biology, Beckman Research Institute, City of Hope, CA 91010</wicri:regionArea></affiliation></author><author><name sortKey="Singer Sam, Judith" sort="Singer Sam, Judith" uniqKey="Singer Sam J" first="Judith" last="Singer-Sam">Judith Singer-Sam</name><affiliation wicri:level="1"><mods:affiliation>Division of Biology, Beckman Research Institute, City of Hope, CA 91010, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Biology, Beckman Research Institute, City of Hope, CA 91010</wicri:regionArea></affiliation><affiliation><mods:affiliation>E-mail: jsam@coh.org</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Mammalian Genome</title><title level="j" type="sub">Incorporating Mouse Genome</title><title level="j" type="abbrev">Mamm Genome</title><idno type="ISSN">0938-8990</idno><idno type="eISSN">1432-1777</idno><imprint><publisher>Springer-Verlag; www.springer-ny.com</publisher><pubPlace>New York</pubPlace><date type="published" when="2004-03-01">2004-03-01</date><biblScope unit="volume">15</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="199">199</biblScope><biblScope unit="page" to="209">209</biblScope></imprint><idno type="ISSN">0938-8990</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0938-8990</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We have developed an imprinting assay combining the use of mice carrying maternal or paternal duplication of chromosomal regions of interest with custom oligonucleotide microarrays. As a model system, we analyzed RNA from CNS tissue of neonatal mice carrying the reciprocal translocation T(7;15)9H and uniparental duplication of proximal Chr 7 and 15. The duplicated region includes the locus on proximal Chr 7 corresponding to the human Prader-Willi/Angelman Syndrome. The microarray contained 322 oligonucleotides, including probes to detect major genes involved in neural excitability and synaptic transmission, as well as known imprinted genes mapping to proximal Chr 7: Ndn, Snrpn, Mkrn3, Magel2, Peg3, and Ube3a. Imprinting of these genes in neonatal cortex and cerebellum was first confirmed by quantitative RT-PCR. Their inclusion on the microarray thus provided positive controls for evaluating the effect of background on the sensitivity of the assay, and for establishing the minimum level of expression required to detect imprinting. Our analysis extended previous work by revealing bi-allelic expression in CNS tissue of those queried genes mapping to proximal Chr 7 or 15, including the Gabrb3 gene, for which there have been conflicting reports. Microarray analysis also revealed no effect of the maternal or paternal disomy on expression levels of the unlinked genes detected, including those potentially implicated in the Prader-Willi or Angelman Syndrome. In addition, quantitative RT-PCR revealed a gene dosage effect in both cerebellum and cortex for all of the known imprinted genes assayed, except for Ube3a in cerebellum.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001958 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 001958 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:0BAE691C77D7E54C579F4AD458BB48F1784B0B33
|texte= Analysis of imprinting in mice with uniparental duplication of proximal chromosomes 7 and 15 by use of a custom oligonucleotide microarray
}}
| This area was generated with Dilib version V0.6.33. |  |