Structure and Function of a Long Alternating Purine-Pyrimidine Sequence in the Mouse Alcohol Dehydrogenase Adh-1 Gene
Identifieur interne : 001039 ( Istex/Curation ); précédent : 001038; suivant : 001040Structure and Function of a Long Alternating Purine-Pyrimidine Sequence in the Mouse Alcohol Dehydrogenase Adh-1 Gene
Auteurs : M. Yu [États-Unis] ; H. J. Edenberg [États-Unis]Source :
- Archives of Biochemistry and Biophysics [ 0003-9861 ] ; 1995.
Abstract
Abstract: The mouse alcohol dehydrogenase gene Adh-1 contains an unusually long alternating purine-pyrimidine sequence within its first intron. This alternating sequence differs in length between strains that differ in the extent of Adh-1 expression, and it has been suggested that it plays a role in gene expression. We demonstrate that this alternating sequence can form Z-DNA in vitro. The alternating sequence can act as a positive regulatory element in transient transfection assays in hepatoma cell lines, but not in CV-1 (monkey kidney) cells, suggesting that it can act as a tissue-specific regulatory element. Nuclear run-on experiments showed that the differential expression of Adh-1 from high- and low-activity strains is, however, controlled at the post-transcription level.
Url:
DOI: 10.1006/abbi.1995.1054
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