A Liquid Chromatographic Preparation of Retroviral RNA
Identifieur interne : 001019 ( Istex/Curation ); précédent : 001018; suivant : 001020A Liquid Chromatographic Preparation of Retroviral RNA
Auteurs : J. Pager [France]Source :
- Analytical Biochemistry [ 0003-2697 ] ; 1993.
Abstract
Abstract: Liquid chromatography was used to prepare native dimer RNA from Moloney murine leukemia retrovirus by gel filtration on a TSK 6000PW column. Three RNA peaks were separated from viral lysate. RNA from the first eluted peak migrated in gel electrophoresis as a native dimer prepared by phenolic extraction and saccharose gradient separation. The last eluted RNA peak likely represents tRNA for proline. HPLC preparation was twice as fast and 20 times more productive than the other method, considering the quantity of pure RNA obtained for the same volume of viral lysate. Using several natural RNAs, it was verified that the dispersion coefficient was inversely proportional to RNA size, at least between 3.6 and 16.6 kb. Within the range of laboratory use, peak surface was in a direct ratio to the injected quantity of a given RNA species. Thus size exclusion chromatography could represent a valuable tool for preparation, analysis, and quantitation of large RNAs.
Url:
DOI: 10.1006/abio.1993.1580
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Liquid Chromatographic Preparation of Retroviral RNA</title><author><name sortKey="Pager, J" sort="Pager, J" uniqKey="Pager J" first="J." last="Pager">J. Pager</name><affiliation wicri:level="1"><mods:affiliation>Inst Gustave Roussy, Biochim Lab, CNRS, LA 147, 39 Rue Camille Desmoulins, F 94805 Villejuif, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Inst Gustave Roussy, Biochim Lab, CNRS, LA 147, 39 Rue Camille Desmoulins, F 94805 Villejuif</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:DDEB40318196ADBC52EE8396224DC4CB75630F7F</idno><date when="1993" year="1993">1993</date><idno type="doi">10.1006/abio.1993.1580</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-817B0J7Q-1/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001019</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001019</idno><idno type="wicri:Area/Istex/Curation">001019</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">A Liquid Chromatographic Preparation of Retroviral RNA</title><author><name sortKey="Pager, J" sort="Pager, J" uniqKey="Pager J" first="J." last="Pager">J. Pager</name><affiliation wicri:level="1"><mods:affiliation>Inst Gustave Roussy, Biochim Lab, CNRS, LA 147, 39 Rue Camille Desmoulins, F 94805 Villejuif, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Inst Gustave Roussy, Biochim Lab, CNRS, LA 147, 39 Rue Camille Desmoulins, F 94805 Villejuif</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Analytical Biochemistry</title><title level="j" type="abbrev">YABIO</title><idno type="ISSN">0003-2697</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">215</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="231">231</biblScope><biblScope unit="page" to="235">235</biblScope></imprint><idno type="ISSN">0003-2697</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-2697</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Liquid chromatography was used to prepare native dimer RNA from Moloney murine leukemia retrovirus by gel filtration on a TSK 6000PW column. Three RNA peaks were separated from viral lysate. RNA from the first eluted peak migrated in gel electrophoresis as a native dimer prepared by phenolic extraction and saccharose gradient separation. The last eluted RNA peak likely represents tRNA for proline. HPLC preparation was twice as fast and 20 times more productive than the other method, considering the quantity of pure RNA obtained for the same volume of viral lysate. Using several natural RNAs, it was verified that the dispersion coefficient was inversely proportional to RNA size, at least between 3.6 and 16.6 kb. Within the range of laboratory use, peak surface was in a direct ratio to the injected quantity of a given RNA species. Thus size exclusion chromatography could represent a valuable tool for preparation, analysis, and quantitation of large RNAs.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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