A Liquid Chromatographic Preparation of Retroviral RNA
Identifieur interne : 001019 ( Istex/Corpus ); précédent : 001018; suivant : 001020A Liquid Chromatographic Preparation of Retroviral RNA
Auteurs : J. PagerSource :
- Analytical Biochemistry [ 0003-2697 ] ; 1993.
Abstract
Abstract: Liquid chromatography was used to prepare native dimer RNA from Moloney murine leukemia retrovirus by gel filtration on a TSK 6000PW column. Three RNA peaks were separated from viral lysate. RNA from the first eluted peak migrated in gel electrophoresis as a native dimer prepared by phenolic extraction and saccharose gradient separation. The last eluted RNA peak likely represents tRNA for proline. HPLC preparation was twice as fast and 20 times more productive than the other method, considering the quantity of pure RNA obtained for the same volume of viral lysate. Using several natural RNAs, it was verified that the dispersion coefficient was inversely proportional to RNA size, at least between 3.6 and 16.6 kb. Within the range of laboratory use, peak surface was in a direct ratio to the injected quantity of a given RNA species. Thus size exclusion chromatography could represent a valuable tool for preparation, analysis, and quantitation of large RNAs.
Url:
DOI: 10.1006/abio.1993.1580
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Pager</name><affiliation><mods:affiliation>Inst Gustave Roussy, Biochim Lab, CNRS, LA 147, 39 Rue Camille Desmoulins, F 94805 Villejuif, France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:DDEB40318196ADBC52EE8396224DC4CB75630F7F</idno><date when="1993" year="1993">1993</date><idno type="doi">10.1006/abio.1993.1580</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-817B0J7Q-1/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001019</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001019</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">A Liquid Chromatographic Preparation of Retroviral RNA</title><author><name sortKey="Pager, J" sort="Pager, J" uniqKey="Pager J" first="J." last="Pager">J. Pager</name><affiliation><mods:affiliation>Inst Gustave Roussy, Biochim Lab, CNRS, LA 147, 39 Rue Camille Desmoulins, F 94805 Villejuif, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Analytical Biochemistry</title><title level="j" type="abbrev">YABIO</title><idno type="ISSN">0003-2697</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">215</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="231">231</biblScope><biblScope unit="page" to="235">235</biblScope></imprint><idno type="ISSN">0003-2697</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-2697</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Liquid chromatography was used to prepare native dimer RNA from Moloney murine leukemia retrovirus by gel filtration on a TSK 6000PW column. Three RNA peaks were separated from viral lysate. RNA from the first eluted peak migrated in gel electrophoresis as a native dimer prepared by phenolic extraction and saccharose gradient separation. The last eluted RNA peak likely represents tRNA for proline. HPLC preparation was twice as fast and 20 times more productive than the other method, considering the quantity of pure RNA obtained for the same volume of viral lysate. Using several natural RNAs, it was verified that the dispersion coefficient was inversely proportional to RNA size, at least between 3.6 and 16.6 kb. Within the range of laboratory use, peak surface was in a direct ratio to the injected quantity of a given RNA species. Thus size exclusion chromatography could represent a valuable tool for preparation, analysis, and quantitation of large RNAs.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>J. 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Three RNA peaks were separated from viral lysate. RNA from the first eluted peak migrated in gel electrophoresis as a native dimer prepared by phenolic extraction and saccharose gradient separation. The last eluted RNA peak likely represents tRNA for proline. HPLC preparation was twice as fast and 20 times more productive than the other method, considering the quantity of pure RNA obtained for the same volume of viral lysate. Using several natural RNAs, it was verified that the dispersion coefficient was inversely proportional to RNA size, at least between 3.6 and 16.6 kb. Within the range of laboratory use, peak surface was in a direct ratio to the injected quantity of a given RNA species. Thus size exclusion chromatography could represent a valuable tool for preparation, analysis, and quantitation of large RNAs.</p></abstract></profileDesc><revisionDesc><change when="1993">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-817B0J7Q-1/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YABIO</jid><aid>71580</aid><ce:pii>S0003-2697(83)71580-0</ce:pii><ce:doi>10.1006/abio.1993.1580</ce:doi><ce:copyright type="full-transfer" year="1993">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>A Liquid Chromatographic Preparation of Retroviral RNA</ce:title><ce:author-group><ce:author><ce:surname>Pager</ce:surname><ce:given-name>J.</ce:given-name></ce:author><ce:affiliation><ce:textfn>Inst Gustave Roussy, Biochim Lab, CNRS, LA 147, 39 Rue Camille Desmoulins, F 94805 Villejuif, France</ce:textfn></ce:affiliation></ce:author-group><ce:abstract class="author"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0010">Liquid chromatography was used to prepare native dimer RNA from Moloney murine leukemia retrovirus by gel filtration on a TSK 6000PW column. Three RNA peaks were separated from viral lysate. RNA from the first eluted peak migrated in gel electrophoresis as a native dimer prepared by phenolic extraction and saccharose gradient separation. The last eluted RNA peak likely represents tRNA for proline. HPLC preparation was twice as fast and 20 times more productive than the other method, considering the quantity of pure RNA obtained for the same volume of viral lysate. Using several natural RNAs, it was verified that the dispersion coefficient was inversely proportional to RNA size, at least between 3.6 and 16.6 kb. Within the range of laboratory use, peak surface was in a direct ratio to the injected quantity of a given RNA species. Thus size exclusion chromatography could represent a valuable tool for preparation, analysis, and quantitation of large RNAs.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>A Liquid Chromatographic Preparation of Retroviral RNA</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>A Liquid Chromatographic Preparation of Retroviral RNA</title></titleInfo><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Pager</namePart><affiliation>Inst Gustave Roussy, Biochim Lab, CNRS, LA 147, 39 Rue Camille Desmoulins, F 94805 Villejuif, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Liquid chromatography was used to prepare native dimer RNA from Moloney murine leukemia retrovirus by gel filtration on a TSK 6000PW column. Three RNA peaks were separated from viral lysate. RNA from the first eluted peak migrated in gel electrophoresis as a native dimer prepared by phenolic extraction and saccharose gradient separation. The last eluted RNA peak likely represents tRNA for proline. HPLC preparation was twice as fast and 20 times more productive than the other method, considering the quantity of pure RNA obtained for the same volume of viral lysate. Using several natural RNAs, it was verified that the dispersion coefficient was inversely proportional to RNA size, at least between 3.6 and 16.6 kb. Within the range of laboratory use, peak surface was in a direct ratio to the injected quantity of a given RNA species. 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