Cloning and sequencing of the replication origin ( ori C) of the Spiroplasma citri chromosome and construction of autonomously replicating artificial plasmids
Identifieur interne : 000259 ( Istex/Curation ); précédent : 000258; suivant : 000260Cloning and sequencing of the replication origin ( ori C) of the Spiroplasma citri chromosome and construction of autonomously replicating artificial plasmids
Auteurs : Fengchun Ye [France] ; Joël Renaudin [France] ; Joseph-Marie Bové [France] ; Frédéric Laigret [France]Source :
- Current Microbiology [ 0343-8651 ] ; 1994-07-01.
English descriptors
- Teeft :
- American society, Amino, Amino acid sequences, Aphidicola, Autonomous replication, Bacillus subtilis, Bacterial chromosome, Buchnera aphidicola, Capricolum, Chromosome, Cirri cells, Cirrus, Citri, Citri cells, Clone, Codon, Coli, Dnaa, Dnaa boxes, Dnaa gene, Dnaa protein, Dnaa region, Dnan, Escherichia, Escherichia coli, Gene, Genome, Gyra, Gyrase, Gyrb, Micrococcus luteus, Molecular biology, Nucleic acids, Nucleotide, Nucleotide sequence, Ogasawara, Other bacteria, Pdna6, Plasmid, Pneumoniae, Pot1, Pot2, Primer, Recf gene, Replication, Replication origin, Sequencing, Spiroplasma, Spiroplasma citri, Spiroplasma citri genome, Subtilis, Taqi, Taqi fragment, Tetm, Tryptophan codon, Yoshikawa.
Abstract
Abstract: A 5.6-kbp fragment ofSpiroplasma citri DNA containing thednaA gene has been cloned and sequenced. Nucleotide sequence analysis shows that this fragment harbors the genes for the replication initiator protein (dnaA), the beta subunit of DNA polymerase III (dnaN), and the DNA gyrase subunits A and B (gyrA andgyrB). The arrangement of these genes,dnaA-dnaN-gyrB-gyrA, is similar to that found in all Gram-positive bacterial genomes studied so far, except that norecF gene was found betweendnaN andgyrB. Several DnaA-box consensus sequences were found upstream ofdnaA and in thednaA-dnaN intergenic region. ThednaA region with the flanking DnaA-boxes and the tetracycline resistance determinant,tetM, were linked into a circular recombinant DNA. This DNA was able to replicate autonomously when introduced by electroporation intoS. citri cells. These experiments show that thednaA region with the DnaA-boxes is the origin of replication ofS. citri and can be used to construct gene vectors.
Url:
DOI: 10.1007/BF01570187
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France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Cellular and Molecular Biology, National Institute of Agronomy Research, and University of Bordeaux II, 33883, Villenave d' Ornon</wicri:regionArea></affiliation></author><author><name sortKey="Laigret, Frederic" sort="Laigret, Frederic" uniqKey="Laigret F" first="Frédéric" last="Laigret">Frédéric Laigret</name><affiliation wicri:level="1"><mods:affiliation>Laboratory of Cellular and Molecular Biology, National Institute of Agronomy Research, and University of Bordeaux II, 33883, Villenave d' Ornon, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Cellular and Molecular Biology, National Institute of Agronomy Research, and University of Bordeaux II, 33883, Villenave d' Ornon</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Current Microbiology</title><title level="j" type="sub">An International 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cells</term><term>Cirrus</term><term>Citri</term><term>Citri cells</term><term>Clone</term><term>Codon</term><term>Coli</term><term>Dnaa</term><term>Dnaa boxes</term><term>Dnaa gene</term><term>Dnaa protein</term><term>Dnaa region</term><term>Dnan</term><term>Escherichia</term><term>Escherichia coli</term><term>Gene</term><term>Genome</term><term>Gyra</term><term>Gyrase</term><term>Gyrb</term><term>Micrococcus luteus</term><term>Molecular biology</term><term>Nucleic acids</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Ogasawara</term><term>Other bacteria</term><term>Pdna6</term><term>Plasmid</term><term>Pneumoniae</term><term>Pot1</term><term>Pot2</term><term>Primer</term><term>Recf gene</term><term>Replication</term><term>Replication origin</term><term>Sequencing</term><term>Spiroplasma</term><term>Spiroplasma citri</term><term>Spiroplasma citri genome</term><term>Subtilis</term><term>Taqi</term><term>Taqi fragment</term><term>Tetm</term><term>Tryptophan 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Nucleotide sequence analysis shows that this fragment harbors the genes for the replication initiator protein (dnaA), the beta subunit of DNA polymerase III (dnaN), and the DNA gyrase subunits A and B (gyrA andgyrB). The arrangement of these genes,dnaA-dnaN-gyrB-gyrA, is similar to that found in all Gram-positive bacterial genomes studied so far, except that norecF gene was found betweendnaN andgyrB. Several DnaA-box consensus sequences were found upstream ofdnaA and in thednaA-dnaN intergenic region. ThednaA region with the flanking DnaA-boxes and the tetracycline resistance determinant,tetM, were linked into a circular recombinant DNA. This DNA was able to replicate autonomously when introduced by electroporation intoS. citri cells. These experiments show that thednaA region with the DnaA-boxes is the origin of replication ofS. citri and can be used to construct gene vectors.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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