Cloning and sequencing of the replication origin ( ori C) of the Spiroplasma citri chromosome and construction of autonomously replicating artificial plasmids
Identifieur interne : 000259 ( Istex/Corpus ); précédent : 000258; suivant : 000260Cloning and sequencing of the replication origin ( ori C) of the Spiroplasma citri chromosome and construction of autonomously replicating artificial plasmids
Auteurs : Fengchun Ye ; Joël Renaudin ; Joseph-Marie Bové ; Frédéric LaigretSource :
- Current Microbiology [ 0343-8651 ] ; 1994-07-01.
English descriptors
- Teeft :
- American society, Amino, Amino acid sequences, Aphidicola, Autonomous replication, Bacillus subtilis, Bacterial chromosome, Buchnera aphidicola, Capricolum, Chromosome, Cirri cells, Cirrus, Citri, Citri cells, Clone, Codon, Coli, Dnaa, Dnaa boxes, Dnaa gene, Dnaa protein, Dnaa region, Dnan, Escherichia, Escherichia coli, Gene, Genome, Gyra, Gyrase, Gyrb, Micrococcus luteus, Molecular biology, Nucleic acids, Nucleotide, Nucleotide sequence, Ogasawara, Other bacteria, Pdna6, Plasmid, Pneumoniae, Pot1, Pot2, Primer, Recf gene, Replication, Replication origin, Sequencing, Spiroplasma, Spiroplasma citri, Spiroplasma citri genome, Subtilis, Taqi, Taqi fragment, Tetm, Tryptophan codon, Yoshikawa.
Abstract
Abstract: A 5.6-kbp fragment ofSpiroplasma citri DNA containing thednaA gene has been cloned and sequenced. Nucleotide sequence analysis shows that this fragment harbors the genes for the replication initiator protein (dnaA), the beta subunit of DNA polymerase III (dnaN), and the DNA gyrase subunits A and B (gyrA andgyrB). The arrangement of these genes,dnaA-dnaN-gyrB-gyrA, is similar to that found in all Gram-positive bacterial genomes studied so far, except that norecF gene was found betweendnaN andgyrB. Several DnaA-box consensus sequences were found upstream ofdnaA and in thednaA-dnaN intergenic region. ThednaA region with the flanking DnaA-boxes and the tetracycline resistance determinant,tetM, were linked into a circular recombinant DNA. This DNA was able to replicate autonomously when introduced by electroporation intoS. citri cells. These experiments show that thednaA region with the DnaA-boxes is the origin of replication ofS. citri and can be used to construct gene vectors.
Url:
DOI: 10.1007/BF01570187
Links to Exploration step
ISTEX:F1074516372ED7167FB9C23431CFD906E0643050Le document en format XML
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Nucleotide sequence analysis shows that this fragment harbors the genes for the replication initiator protein (dnaA), the beta subunit of DNA polymerase III (dnaN), and the DNA gyrase subunits A and B (gyrA andgyrB). The arrangement of these genes,dnaA-dnaN-gyrB-gyrA, is similar to that found in all Gram-positive bacterial genomes studied so far, except that norecF gene was found betweendnaN andgyrB. Several DnaA-box consensus sequences were found upstream ofdnaA and in thednaA-dnaN intergenic region. ThednaA region with the flanking DnaA-boxes and the tetracycline resistance determinant,tetM, were linked into a circular recombinant DNA. This DNA was able to replicate autonomously when introduced by electroporation intoS. citri cells. 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Nucleotide sequence analysis shows that this fragment harbors the genes for the replication initiator protein (dnaA), the beta subunit of DNA polymerase III (dnaN), and the DNA gyrase subunits A and B (gyrA andgyrB). The arrangement of these genes,dnaA-dnaN-gyrB-gyrA, is similar to that found in all Gram-positive bacterial genomes studied so far, except that norecF gene was found betweendnaN andgyrB. Several DnaA-box consensus sequences were found upstream ofdnaA and in thednaA-dnaN intergenic region. ThednaA region with the flanking DnaA-boxes and the tetracycline resistance determinant,tetM, were linked into a circular recombinant DNA. This DNA was able to replicate autonomously when introduced by electroporation intoS. citri cells. These experiments show that thednaA region with the DnaA-boxes is the origin of replication ofS. citri and can be used to construct gene vectors.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Biotechnology</term></item><item><term>Microbiology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1994-07-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-1B7CVXHH-C/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" 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plasmids</ArticleTitle><ArticleFirstPage>23</ArticleFirstPage><ArticleLastPage>29</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2005</Year><Month>4</Month><Day>12</Day></RegistrationDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1994</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>284</JournalID><VolumeIDStart>29</VolumeIDStart><VolumeIDEnd>29</VolumeIDEnd><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1" PresentAffiliationID="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Fengchun</GivenName><FamilyName>Ye</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Joël</GivenName><FamilyName>Renaudin</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><Prefix>Dr.</Prefix><GivenName>Joseph-Marie</GivenName><FamilyName>Bové</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Frédéric</GivenName><FamilyName>Laigret</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Laboratory of Cellular and Molecular Biology</OrgDivision><OrgName>National Institute of Agronomy Research, and University of Bordeaux II</OrgName><OrgAddress><Postcode>33883</Postcode><City>Villenave d' Ornon</City><Country>France</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Department of Plant Pathology</OrgDivision><OrgName>Oklahoma State University, 110 Noble Research Center</OrgName><OrgAddress><Postcode>74078</Postcode><City>Stillwater</City><State>OK</State><Country>USA</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>A 5.6-kbp fragment of<Emphasis Type="Italic">Spiroplasma citri</Emphasis> DNA containing the<Emphasis Type="Italic">dna</Emphasis>A gene has been cloned and sequenced. Nucleotide sequence analysis shows that this fragment harbors the genes for the replication initiator protein (<Emphasis Type="Italic">dna</Emphasis>A), the beta subunit of DNA polymerase III (<Emphasis Type="Italic">dna</Emphasis>N), and the DNA gyrase subunits A and B (<Emphasis Type="Italic">gyr</Emphasis>A and<Emphasis Type="Italic">gyr</Emphasis>B). The arrangement of these genes,<Emphasis Type="Italic">dna</Emphasis>A-<Emphasis Type="Italic">dna</Emphasis>N-<Emphasis Type="Italic">gyr</Emphasis>B-<Emphasis Type="Italic">gyr</Emphasis>A, is similar to that found in all Gram-positive bacterial genomes studied so far, except that no<Emphasis Type="Italic">rec</Emphasis>F gene was found between<Emphasis Type="Italic">dna</Emphasis>N and<Emphasis Type="Italic">gyr</Emphasis>B. Several DnaA-box consensus sequences were found upstream of<Emphasis Type="Italic">dna</Emphasis>A and in the<Emphasis Type="Italic">dna</Emphasis>A-<Emphasis Type="Italic">dna</Emphasis>N intergenic region. The<Emphasis Type="Italic">dna</Emphasis>A region with the flanking DnaA-boxes and the tetracycline resistance determinant,<Emphasis Type="Italic">tet</Emphasis>M, were linked into a circular recombinant DNA. This DNA was able to replicate autonomously when introduced by electroporation into<Emphasis Type="Italic">S. citri</Emphasis> cells. These experiments show that the<Emphasis Type="Italic">dna</Emphasis>A region with the DnaA-boxes is the origin of replication of<Emphasis Type="Italic">S. citri</Emphasis> and can be used to construct gene vectors.</Para></Abstract></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Cloning and sequencing of the replication origin ( ori C) of the Spiroplasma citri chromosome and construction of autonomously replicating artificial plasmids</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Cloning and sequencing of the replication origin ( ori C) of the Spiroplasma citri chromosome and construction of autonomously replicating artificial plasmids</title></titleInfo><name type="personal"><namePart type="given">Fengchun</namePart><namePart type="family">Ye</namePart><affiliation>Laboratory of Cellular and Molecular Biology, National Institute of Agronomy Research, and University of Bordeaux II, 33883, Villenave d' Ornon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Joël</namePart><namePart type="family">Renaudin</namePart><affiliation>Laboratory of Cellular and Molecular Biology, National Institute of Agronomy Research, and University of Bordeaux II, 33883, Villenave d' Ornon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="termsOfAddress">Dr.</namePart><namePart type="given">Joseph-Marie</namePart><namePart type="family">Bové</namePart><affiliation>Laboratory of Cellular and Molecular Biology, National Institute of Agronomy Research, and University of Bordeaux II, 33883, Villenave d' Ornon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Frédéric</namePart><namePart type="family">Laigret</namePart><affiliation>Laboratory of Cellular and Molecular Biology, National Institute of Agronomy Research, and University of Bordeaux II, 33883, Villenave d' Ornon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">1994-07-01</dateIssued><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: A 5.6-kbp fragment ofSpiroplasma citri DNA containing thednaA gene has been cloned and sequenced. Nucleotide sequence analysis shows that this fragment harbors the genes for the replication initiator protein (dnaA), the beta subunit of DNA polymerase III (dnaN), and the DNA gyrase subunits A and B (gyrA andgyrB). The arrangement of these genes,dnaA-dnaN-gyrB-gyrA, is similar to that found in all Gram-positive bacterial genomes studied so far, except that norecF gene was found betweendnaN andgyrB. Several DnaA-box consensus sequences were found upstream ofdnaA and in thednaA-dnaN intergenic region. ThednaA region with the flanking DnaA-boxes and the tetracycline resistance determinant,tetM, were linked into a circular recombinant DNA. This DNA was able to replicate autonomously when introduced by electroporation intoS. citri cells. These experiments show that thednaA region with the DnaA-boxes is the origin of replication ofS. citri and can be used to construct gene vectors.</abstract><relatedItem type="host"><titleInfo><title>Current Microbiology</title><subTitle>An International Journal</subTitle></titleInfo><titleInfo type="abbreviated"><title>Current Microbiology</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1994-07-01</dateIssued><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Biotechnology</topic><topic>Microbiology</topic></subject><identifier type="ISSN">0343-8651</identifier><identifier type="eISSN">1432-0991</identifier><identifier type="JournalID">284</identifier><identifier type="IssueArticleCount">11</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>1994</date><detail type="volume"><number>29</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="pages"><start>23</start><end>29</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1994</recordOrigin></recordInfo></relatedItem><identifier type="istex">F1074516372ED7167FB9C23431CFD906E0643050</identifier><identifier type="ark">ark:/67375/1BB-1B7CVXHH-C</identifier><identifier type="DOI">10.1007/BF01570187</identifier><identifier type="ArticleID">BF01570187</identifier><identifier type="ArticleID">Art5</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1994</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 1994</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-1B7CVXHH-C/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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