Hydrogen-bonding Contacts in the Major Groove are required for Human Immunodeficiency Virus Type-1 tat Protein Recognition of TAR RNA
Identifieur interne : 000230 ( Istex/Curation ); précédent : 000229; suivant : 000231Hydrogen-bonding Contacts in the Major Groove are required for Human Immunodeficiency Virus Type-1 tat Protein Recognition of TAR RNA
Auteurs : François Hamy [Royaume-Uni] ; Ulysse Asseline [Royaume-Uni] ; Jane Grasby [Royaume-Uni] ; Shigenori Iwai [Royaume-Uni] ; Clare Pritchard [Royaume-Uni] ; George Slim [Royaume-Uni] ; P. Jonathan G. Butler [Royaume-Uni] ; Jonathan Karn [Royaume-Uni] ; Michael J. Gait [Royaume-Uni]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 1993.
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Abstract
Abstract: The binding site for tat on TAR RNA was analysed by preparing a series of model RNA substrates carrying site-specific functional group modifications. The test RNAs were prepared by annealing two short synthetic oligoribonucleotides to form a duplex structure with a U-rich bulge and flanking sequences identical to TAR RNA. Tat binds these duplex RNAs with approximately half the affinity for wild-type TAR RNA. Substitution at positions U23 or U25 by the base analogue. O4-methyl-dT, which is deficient in its ability to hydrogen-bond at the N3 position reduces tat affinity more than 20-fold. Modifications to purines in the stem of TAR RNA that affect hydrogen-bonding ability in either the major or the minor groove of duplex RNA were also tested. Removal of the nitrogen atom at either the N7 position of G26 or the N7 position of A27 reduces tat affinity 10- to 20-fold. By contrast removal of the exocyclic amino group in the minor groove at position G26, by substitution with inosine, does not affect tat binding significantly. A single methylphosphonate substitution at the phosphate bond between A22 and U23 also leads to a significant loss of tat binding ability, whereas all other methylphosphonate substitutions in the U-rich bulge are not harmful to tat binding. We conclude that tat forms multiple specific hydrogen bonds to a series of dispersed sites displayed in the major groove of the TAR RNA molecule. These include the N3-H of U23, the N7 of G26, the N7 of A26 and the phosphate between A22 and U23.
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DOI: 10.1006/jmbi.1993.1129
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<term>TAR RNA structure</term>
<term>human immunodeficiency virus type 1 (HIV-1)</term>
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<term>trans-activation</term>
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<front><div type="abstract" xml:lang="en">Abstract: The binding site for tat on TAR RNA was analysed by preparing a series of model RNA substrates carrying site-specific functional group modifications. The test RNAs were prepared by annealing two short synthetic oligoribonucleotides to form a duplex structure with a U-rich bulge and flanking sequences identical to TAR RNA. Tat binds these duplex RNAs with approximately half the affinity for wild-type TAR RNA. Substitution at positions U23 or U25 by the base analogue. O4-methyl-dT, which is deficient in its ability to hydrogen-bond at the N3 position reduces tat affinity more than 20-fold. Modifications to purines in the stem of TAR RNA that affect hydrogen-bonding ability in either the major or the minor groove of duplex RNA were also tested. Removal of the nitrogen atom at either the N7 position of G26 or the N7 position of A27 reduces tat affinity 10- to 20-fold. By contrast removal of the exocyclic amino group in the minor groove at position G26, by substitution with inosine, does not affect tat binding significantly. A single methylphosphonate substitution at the phosphate bond between A22 and U23 also leads to a significant loss of tat binding ability, whereas all other methylphosphonate substitutions in the U-rich bulge are not harmful to tat binding. We conclude that tat forms multiple specific hydrogen bonds to a series of dispersed sites displayed in the major groove of the TAR RNA molecule. These include the N3-H of U23, the N7 of G26, the N7 of A26 and the phosphate between A22 and U23.</div>
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