T cell responses to peptides covering the gag p24 region of HIV -1 occur in HIV -1 seronegative individuals
Identifieur interne : 000025 ( Istex/Curation ); précédent : 000024; suivant : 000026T cell responses to peptides covering the gag p24 region of HIV -1 occur in HIV -1 seronegative individuals
Auteurs : Annapurna Vyakarnam [Suède, Royaume-Uni] ; Patricia M. Matear [Suède] ; Charmaine Cranenburg [Royaume-Uni] ; Colin Michie [Royaume-Uni] ; Peter C. L. Beverley [Royaume-Uni] ; Britta Wahren [Suède] ; Sarah K. Gill [Suède] ; Ian Weller [Suède]Source :
- International Immunology [ 0953-8178 ] ; 1991.
Abstract
We demonstrate that peptides (16 amino acids long) covering the sequence of the HIV-1 core protein p24 induce significant proliferation in peripheral blood mononuclear cells (PBMC) of several (>50%) healthy seronegative volunteers as well as seronegative homosexual men. The nature of this response was characterized and compared with those of HIV-infected patients. Several peptides induced responses; however, the most frequent responses in both seropositive and seronegative individuals were noted to the following peptides: 1 and 2 (aa 133–157); 6 and 7 (aa 183–207); 15 (aa 273–287); and 17 and 18 (aa 293–317). The response pattern was related to the disease stage of the patients; seronegative individuals as well as asymptomatic seropositive individuals (CDC II/III) responded to low concentrations of several peptides, but symptomatic patients (CDC IV) only responded to high concentrations of a few peptides. Cell separation studies of PBMC from healthy volunteers showed that the responding cells were CD4+ and expressed the CD45RO differentiation antigen. Furthermore, cord-blood mononuclear cells with <5% of CD45RO T cells did not proliferative to any of the peptides. Finally, CD4+ T cell lines specific for both peptides and p24 protein were successfully established from the PBMC of seronegative individuals confirming the data obtained with freshly isolated cells. These studies therefore suggest that the CD4+ T cell response to p24 is not strictly disease related, instead, the response may be due to priming of the host with cross-reactive antigens.
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DOI: 10.1093/intimm/3.10.939
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Matear</name><affiliation wicri:level="1"><mods:affiliation>Academic Department of Genito-Urinary Medicine, University College London, London UK, London UK, London UK, Stockholm, Sweden</mods:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Academic Department of Genito-Urinary Medicine, University College London, London UK, London UK, London UK, Stockholm</wicri:regionArea></affiliation></author><author><name sortKey="Cranenburg, Charmaine" sort="Cranenburg, Charmaine" uniqKey="Cranenburg C" first="Charmaine" last="Cranenburg">Charmaine Cranenburg</name><affiliation wicri:level="1"><mods:affiliation>Department of Immunology, University College London London UK</mods:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Immunology</wicri:regionArea></affiliation></author><author><name sortKey="Michie, Colin" sort="Michie, Colin" uniqKey="Michie C" first="Colin" last="Michie">Colin Michie</name><affiliation wicri:level="1"><mods:affiliation>ICRF Human Tumour Immunology Group, University College London London UK</mods:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>ICRF Human Tumour Immunology Group</wicri:regionArea></affiliation></author><author><name sortKey="Beverley, Peter C L" sort="Beverley, Peter C L" uniqKey="Beverley P" first="Peter C. 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Gill</name><affiliation wicri:level="1"><mods:affiliation>Academic Department of Genito-Urinary Medicine, University College London, London UK, London UK, London UK, Stockholm, Sweden</mods:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Academic Department of Genito-Urinary Medicine, University College London, London UK, London UK, London UK, Stockholm</wicri:regionArea></affiliation></author><author><name sortKey="Weller, Ian" sort="Weller, Ian" uniqKey="Weller I" first="Ian" last="Weller">Ian Weller</name><affiliation wicri:level="1"><mods:affiliation>Academic Department of Genito-Urinary Medicine, University College London, London UK, London UK, London UK, Stockholm, Sweden</mods:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Academic Department of Genito-Urinary Medicine, University College London, London UK, London UK, London UK, Stockholm</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">International Immunology</title><idno type="eISSN">1460-2377</idno><idno type="ISSN">0953-8178</idno><imprint><publisher>Oxford University Press</publisher><date type="published">1991</date><biblScope unit="vol">3</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="939">939</biblScope><biblScope unit="page" to="947">947</biblScope></imprint><idno type="ISSN">0953-8178</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0953-8178</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">We demonstrate that peptides (16 amino acids long) covering the sequence of the HIV-1 core protein p24 induce significant proliferation in peripheral blood mononuclear cells (PBMC) of several (>50%) healthy seronegative volunteers as well as seronegative homosexual men. The nature of this response was characterized and compared with those of HIV-infected patients. Several peptides induced responses; however, the most frequent responses in both seropositive and seronegative individuals were noted to the following peptides: 1 and 2 (aa 133–157); 6 and 7 (aa 183–207); 15 (aa 273–287); and 17 and 18 (aa 293–317). The response pattern was related to the disease stage of the patients; seronegative individuals as well as asymptomatic seropositive individuals (CDC II/III) responded to low concentrations of several peptides, but symptomatic patients (CDC IV) only responded to high concentrations of a few peptides. Cell separation studies of PBMC from healthy volunteers showed that the responding cells were CD4+ and expressed the CD45RO differentiation antigen. Furthermore, cord-blood mononuclear cells with <5% of CD45RO T cells did not proliferative to any of the peptides. Finally, CD4+ T cell lines specific for both peptides and p24 protein were successfully established from the PBMC of seronegative individuals confirming the data obtained with freshly isolated cells. These studies therefore suggest that the CD4+ T cell response to p24 is not strictly disease related, instead, the response may be due to priming of the host with cross-reactive antigens.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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