Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis
Identifieur interne : 001E45 ( Istex/Corpus ); précédent : 001E44; suivant : 001E46Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis
Auteurs : James C. Stone ; Tom Atkinson ; Michael Smith ; Tony PawsonSource :
- Cell [ 0092-8674 ] ; 1984.
English descriptors
- Teeft :
- Agar, Amino acid residues, Catalytic domain, Cell lines, Cellular proteins, Cellular transformation, Central position, Coding sequence, Deletion, Digestion, England biolabs, Ethidium bromide, Experimental procedures, Fibroblast, Fibroblast transformation, Fujinami, Fujinami sarcoma virus, Functional domains, Functional regions, Further analysis, Further study, Genome, Hanafusa, Insertion, Insertion mutagenesis, Insertion mutants, Insertion mutations, Kinase, Kinase activity, Kinase reaction, Linear molecules, Linker, Linker deletion, Linker mutations, Local disruption, Locus, Mammalian cells, Medical research council, Monolinker mutation, Morphological transformation, Mutagenesis, Mutant, Mutation, National cancer institute, Nucleotide sequence, Oncogene, Oncogene product, Peptide insertion, Personal communication, Phosphorylation, Plasmid, Primary structure, Protein kinase activity, Reading frame, Recognition sequence, Regular medium, Restriction sites, Rous sarcoma virus, Sarcoma, Shibuya, Small colonies, Soft agar, Thymidine kinase gene, Transfection, Transfection experiments, Viral, Viral protein.
Abstract
Abstract: A novel mutagenesis procedure based on the insertion of a hexameric nucleotide sequence into Rsa I restriction sites of cloned DNA has been applied to a copy of the Fujinami sarcoma virus (FSV) genome with the aim of identifying functional regions in the transforming protein. Mutations specifying peptide insertions in both the NH2- and the COOH-terminal fps-specific portions of the transforming protein reduce or abolish the capacity of the genome to induce transformed foci in rat-2 cells. Insertion of multiple copies of the hexamer into one central position in the oncogene results in dislocation of the NH2- and COOH-terminal regions in the primary structure, but has no inhibitory effect on focus induction. Taken together, the results imply that both the NH2- and COOH-terminal fps-specific portions of the FSV oncogene product possess determinants which function in fibroblast transformation, and that cooperation of these two regions is not sensitive to their separation in the primary structure.
Url:
DOI: 10.1016/0092-8674(84)90385-4
Links to Exploration step
ISTEX:9C8A70F9B8A7B7B1C12E7B56F0760924634D3C16Le document en format XML
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Mutations specifying peptide insertions in both the NH2- and the COOH-terminal fps-specific portions of the transforming protein reduce or abolish the capacity of the genome to induce transformed foci in rat-2 cells. Insertion of multiple copies of the hexamer into one central position in the oncogene results in dislocation of the NH2- and COOH-terminal regions in the primary structure, but has no inhibitory effect on focus induction. 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Stone</name><affiliations><json:string>Department of Microbiology University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</json:string></affiliations></json:item><json:item><name>Tom Atkinson</name><affiliations><json:string>Department of Biochemistry University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</json:string></affiliations></json:item><json:item><name>Michael Smith</name><affiliations><json:string>Department of Biochemistry University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</json:string></affiliations></json:item><json:item><name>Tony Pawson</name><affiliations><json:string>Department of Microbiology University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-5T4VSB05-G</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: A novel mutagenesis procedure based on the insertion of a hexameric nucleotide sequence into Rsa I restriction sites of cloned DNA has been applied to a copy of the Fujinami sarcoma virus (FSV) genome with the aim of identifying functional regions in the transforming protein. Mutations specifying peptide insertions in both the NH2- and the COOH-terminal fps-specific portions of the transforming protein reduce or abolish the capacity of the genome to induce transformed foci in rat-2 cells. Insertion of multiple copies of the hexamer into one central position in the oncogene results in dislocation of the NH2- and COOH-terminal regions in the primary structure, but has no inhibitory effect on focus induction. Taken together, the results imply that both the NH2- and COOH-terminal fps-specific portions of the FSV oncogene product possess determinants which function in fibroblast transformation, and that cooperation of these two regions is not sensitive to their separation in the primary structure.</abstract><qualityIndicators><score>8.86</score><pdfWordCount>6184</pdfWordCount><pdfCharCount>40083</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>10</pdfPageCount><pdfPageSize>598 x 812.56 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>155</abstractWordCount><abstractCharCount>1017</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis</title><pmid><json:string>6327075</json:string></pmid><pii><json:string>0092-8674(84)90385-4</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Cold Spring Harbor Symp. 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Auersperg</json:string><json:string>Joan McPherson</json:string><json:string>Gerry Weinmaster</json:string><json:string>C. Huang</json:string><json:string>Ross MacGrllivray</json:string><json:string>U.S.C. Section</json:string><json:string>H. Hanafusa</json:string><json:string>C. Seimans</json:string><json:string>W. Topp</json:string><json:string>D. Bolognesi</json:string><json:string>M. Shrbuya</json:string><json:string>C. Transformation</json:string><json:string>To</json:string><json:string>Michael Smith</json:string><json:string>R. C. Miller</json:string><json:string>Nancy Dower</json:string><json:string>Tony Pawson</json:string><json:string>Mark Zoller</json:string><json:string>M. Shlbuya</json:string><json:string>Tom Atkinson</json:string></persName><placeName></placeName><ref_url></ref_url><ref_bibl><json:string>Sanger et al., 1977</json:string><json:string>Heffron et al., 1978</json:string><json:string>Cooper et al., 1984</json:string><json:string>Heffron et al. (1978)</json:string><json:string>Lee et al., 1983</json:string><json:string>Shlbuya and Hanafusa, 1982</json:string><json:string>Messing, 1983</json:string><json:string>Neil et al., 1981</json:string><json:string>Topp, 1981</json:string><json:string>Breitman et al., 1981</json:string><json:string>Dagert and Ehrlich (1979)</json:string><json:string>Kitamura and Yoshida, 1983</json:string><json:string>Mathey-Prevot et al., 1982</json:string><json:string>Feldman et al., 1980</json:string><json:string>Hanafusa et al. (1981)</json:string><json:string>Graham et al. (1980)</json:string><json:string>Shibuya and Hanafusa, 1982</json:string><json:string>Lee et al., 1981</json:string><json:string>Levtnson et al., 1981</json:string><json:string>Enqulst et al., 1979</json:string><json:string>Foster and Hanafusa (1983)</json:string><json:string>Hanafusa et al., 1981</json:string><json:string>Levinson et al., 1981</json:string><json:string>Shibuya et al., 1982</json:string><json:string>Weinmaster et al. (1983)</json:string><json:string>Wong et al., 1980</json:string><json:string>McDonnel et al. (1977)</json:string><json:string>Cooper and Hunter, 1981</json:string><json:string>Campbell et al., 1978</json:string><json:string>Feldman et al., 1983</json:string><json:string>Tatchell et al., 1981</json:string><json:string>Birnboim and Doly, 1979</json:string><json:string>Krueger et al., 1982</json:string><json:string>Prywes et al. (1983)</json:string><json:string>Messelson and Yuan, 1968</json:string><json:string>Adams et al., 1983</json:string><json:string>Wigler et al. (1979)</json:string><json:string>Pawson et al., 1980</json:string><json:string>Weinmaster et al., 1983</json:string><json:string>Holmes and Quigley, 1981</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-5T4VSB05-G</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - cell biology</json:string><json:string>2 - biochemistry & molecular biology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - developmental biology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - General Biochemistry, Genetics and Molecular Biology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - generalites</json:string></inist></categories><publicationDate>1984</publicationDate><copyrightDate>1984</copyrightDate><doi><json:string>10.1016/0092-8674(84)90385-4</json:string></doi><id>9C8A70F9B8A7B7B1C12E7B56F0760924634D3C16</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5T4VSB05-G/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5T4VSB05-G/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-5T4VSB05-G/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1984</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis</title><author xml:id="author-0000"><persName><forename type="first">James C.</forename><surname>Stone</surname></persName><affiliation>Department of Microbiology University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Tom</forename><surname>Atkinson</surname></persName><affiliation>Department of Biochemistry University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Michael</forename><surname>Smith</surname></persName><affiliation>Department of Biochemistry University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Tony</forename><surname>Pawson</surname></persName><affiliation>Department of Microbiology University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</affiliation></author><idno type="istex">9C8A70F9B8A7B7B1C12E7B56F0760924634D3C16</idno><idno type="ark">ark:/67375/6H6-5T4VSB05-G</idno><idno type="DOI">10.1016/0092-8674(84)90385-4</idno><idno type="PII">0092-8674(84)90385-4</idno></analytic><monogr><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="pISSN">0092-8674</idno><idno type="PII">S0092-8674(00)X0555-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1984"></date><biblScope unit="volume">37</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="549">549</biblScope><biblScope unit="page" to="558">558</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1984</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: A novel mutagenesis procedure based on the insertion of a hexameric nucleotide sequence into Rsa I restriction sites of cloned DNA has been applied to a copy of the Fujinami sarcoma virus (FSV) genome with the aim of identifying functional regions in the transforming protein. Mutations specifying peptide insertions in both the NH2- and the COOH-terminal fps-specific portions of the transforming protein reduce or abolish the capacity of the genome to induce transformed foci in rat-2 cells. Insertion of multiple copies of the hexamer into one central position in the oncogene results in dislocation of the NH2- and COOH-terminal regions in the primary structure, but has no inhibitory effect on focus induction. Taken together, the results imply that both the NH2- and COOH-terminal fps-specific portions of the FSV oncogene product possess determinants which function in fibroblast transformation, and that cooperation of these two regions is not sensitive to their separation in the primary structure.</p></abstract></profileDesc><revisionDesc><change when="1984-03-19">Modified</change><change when="1984">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5T4VSB05-G/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>CELL</jid><aid>84903854</aid><ce:pii>0092-8674(84)90385-4</ce:pii><ce:doi>10.1016/0092-8674(84)90385-4</ce:doi><ce:copyright type="unknown" year="1984"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Article</ce:textfn></ce:dochead><ce:title>Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis</ce:title><ce:author-group><ce:author><ce:given-name>James C.</ce:given-name><ce:surname>Stone</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Tom</ce:given-name><ce:surname>Atkinson</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Michael</ce:given-name><ce:surname>Smith</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Tony</ce:given-name><ce:surname>Pawson</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Department of Microbiology University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Department of Biochemistry University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="30" month="1" year="1984"></ce:date-received><ce:date-revised day="19" month="3" year="1984"></ce:date-revised><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A novel mutagenesis procedure based on the insertion of a hexameric nucleotide sequence into Rsa I restriction sites of cloned DNA has been applied to a copy of the Fujinami sarcoma virus (FSV) genome with the aim of identifying functional regions in the transforming protein. Mutations specifying peptide insertions in both the NH<ce:inf>2</ce:inf>- and the COOH-terminal <ce:italic>fps</ce:italic>-specific portions of the transforming protein reduce or abolish the capacity of the genome to induce transformed foci in rat-2 cells. Insertion of multiple copies of the hexamer into one central position in the oncogene results in dislocation of the NH<ce:inf>2</ce:inf>- and COOH-terminal regions in the primary structure, but has no inhibitory effect on focus induction. Taken together, the results imply that both the NH<ce:inf>2</ce:inf>- and COOH-terminal <ce:italic>fps</ce:italic>-specific portions of the FSV oncogene product possess determinants which function in fibroblast transformation, and that cooperation of these two regions is not sensitive to their separation in the primary structure.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis</title></titleInfo><name type="personal"><namePart type="given">James C.</namePart><namePart type="family">Stone</namePart><affiliation>Department of Microbiology University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tom</namePart><namePart type="family">Atkinson</namePart><affiliation>Department of Biochemistry University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Michael</namePart><namePart type="family">Smith</namePart><affiliation>Department of Biochemistry University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tony</namePart><namePart type="family">Pawson</namePart><affiliation>Department of Microbiology University of British Columbia Vancouver, British Columbia, Canada V6T 1W5</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1984</dateIssued><dateModified encoding="w3cdtf">1984-03-19</dateModified><copyrightDate encoding="w3cdtf">1984</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: A novel mutagenesis procedure based on the insertion of a hexameric nucleotide sequence into Rsa I restriction sites of cloned DNA has been applied to a copy of the Fujinami sarcoma virus (FSV) genome with the aim of identifying functional regions in the transforming protein. Mutations specifying peptide insertions in both the NH2- and the COOH-terminal fps-specific portions of the transforming protein reduce or abolish the capacity of the genome to induce transformed foci in rat-2 cells. Insertion of multiple copies of the hexamer into one central position in the oncogene results in dislocation of the NH2- and COOH-terminal regions in the primary structure, but has no inhibitory effect on focus induction. Taken together, the results imply that both the NH2- and COOH-terminal fps-specific portions of the FSV oncogene product possess determinants which function in fibroblast transformation, and that cooperation of these two regions is not sensitive to their separation in the primary structure.</abstract><note type="content">Section title: Article</note><relatedItem type="host"><titleInfo><title>Cell</title></titleInfo><titleInfo type="abbreviated"><title>CELL</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1984</dateIssued></originInfo><identifier type="ISSN">0092-8674</identifier><identifier type="PII">S0092-8674(00)X0555-7</identifier><part><date>1984</date><detail type="volume"><number>37</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>349</start><end>695</end></extent><extent unit="pages"><start>549</start><end>558</end></extent></part></relatedItem><identifier type="istex">9C8A70F9B8A7B7B1C12E7B56F0760924634D3C16</identifier><identifier type="ark">ark:/67375/6H6-5T4VSB05-G</identifier><identifier type="DOI">10.1016/0092-8674(84)90385-4</identifier><identifier type="PII">0092-8674(84)90385-4</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5T4VSB05-G/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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