MersV1, Istex, Corpus, bibRecord, 001E14

Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner

Identifieur interne : 001E14 ( Istex/Corpus ); précédent : 001E13; suivant : 001E15

Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner

Auteurs : Maria R. Rojas ; Amine O. Noueiry ; William J. Lucas ; Robert L. Gilbertson

Source :

Cell [ 0092-8674 ] ; 1998.

RBID : ISTEX:0BC8612A0715E465962B785D74463947AAA69932

English descriptors

Teeft :
- Agarose, Assay, Bdmv, Bdmv fragment, Bean dwarf mosaic geminivirus, Bind, Bind dsdna, Bind ssdna, Binding buffer, Binding properties, Binding studies, Bipartite, Bipartite geminiviruses, Circular forms, Circular ssdna, Coli proteins, Common region, Direct interaction, Dna, Dsdna, Final volume, Geminivirus, Geminiviruses, Genome, Genome size, Genomic, Genomic size, Gilbertson, Linear dsdna, Linear dsdna ladder, Linear forms, Lucas, Microinjection studies, Mobility, Mosaic, Movement protein, Movement proteins, Nondenaturing, Nondenaturing agarose, Noueiry, Nucleic, Nucleic acids, Pbda1, Plant cell, Plasmodesma, Plasmodesmal boundaries, Protein, Renatured, Rojas, Room temperature, Sequence specificity, Southern blot hybridization analysis, Ssdna, Supercoiled, Supercoiled form, Systemic infection, Viral, Viral movement, Virol, Virology.

Abstract

Abstract: Plant viral movement proteins mediate the cell-to-cell movement of nucleic acids. This involves either a direct interaction between the viral movement protein and the nucleic acid or an indirect interaction involving host factors. The bipartite geminiviruses possess two movement proteins, BV1 and BC1, that coordinate movement of viral DNA across nuclear and plasmodesmal boundaries, respectively. Here, we demonstrate that both BV1 and BC1 interact directly with DNA and, in addition, that they have the unique property to recognize DNA on the basis of form and size rather than sequence. This is a novel feature for plant virus movement proteins and raises the possibility that BV1 and BC1 may be determinants of genome size in the bipartite geminiviruses.

Url:

https://api.istex.fr/ark:/67375/6H6-9NJW2TSN-8/fulltext.pdf

DOI: 10.1016/S0092-8674(00)81786-9

Links to Exploration step

ISTEX:0BC8612A0715E465962B785D74463947AAA69932

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner</title>
<author><name sortKey="Rojas, Maria R" sort="Rojas, Maria R" uniqKey="Rojas M" first="Maria R" last="Rojas">Maria R. Rojas</name>
<affiliation><mods:affiliation>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Noueiry, Amine O" sort="Noueiry, Amine O" uniqKey="Noueiry A" first="Amine O" last="Noueiry">Amine O. Noueiry</name>
<affiliation><mods:affiliation>Department of Plant Pathology, University of California, Davis, Davis, California 95616, USA</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>§ Present address: Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin 53706.</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Lucas, William J" sort="Lucas, William J" uniqKey="Lucas W" first="William J" last="Lucas">William J. Lucas</name>
<affiliation><mods:affiliation>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Gilbertson, Robert L" sort="Gilbertson, Robert L" uniqKey="Gilbertson R" first="Robert L" last="Gilbertson">Robert L. Gilbertson</name>
<affiliation><mods:affiliation>Department of Plant Pathology, University of California, Davis, Davis, California 95616, USA</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>Correspondence: Robert L. Gilbertson, 530 752 3163 (phone), 530 752 5674 (fax)</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>‡ To whom correspondence should be addressed (e-mail: rlgilbertson@ucdavis.edu).</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>E-mail: rlgilbertson@ucdavis.edu</mods:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:0BC8612A0715E465962B785D74463947AAA69932</idno>
<date when="1998" year="1998">1998</date>
<idno type="doi">10.1016/S0092-8674(00)81786-9</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-9NJW2TSN-8/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001E14</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001E14</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner</title>
<author><name sortKey="Rojas, Maria R" sort="Rojas, Maria R" uniqKey="Rojas M" first="Maria R" last="Rojas">Maria R. Rojas</name>
<affiliation><mods:affiliation>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Noueiry, Amine O" sort="Noueiry, Amine O" uniqKey="Noueiry A" first="Amine O" last="Noueiry">Amine O. Noueiry</name>
<affiliation><mods:affiliation>Department of Plant Pathology, University of California, Davis, Davis, California 95616, USA</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>§ Present address: Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin 53706.</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Lucas, William J" sort="Lucas, William J" uniqKey="Lucas W" first="William J" last="Lucas">William J. Lucas</name>
<affiliation><mods:affiliation>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Gilbertson, Robert L" sort="Gilbertson, Robert L" uniqKey="Gilbertson R" first="Robert L" last="Gilbertson">Robert L. Gilbertson</name>
<affiliation><mods:affiliation>Department of Plant Pathology, University of California, Davis, Davis, California 95616, USA</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>Correspondence: Robert L. Gilbertson, 530 752 3163 (phone), 530 752 5674 (fax)</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>‡ To whom correspondence should be addressed (e-mail: rlgilbertson@ucdavis.edu).</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>E-mail: rlgilbertson@ucdavis.edu</mods:affiliation>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Cell</title>
<title level="j" type="abbrev">CELL</title>
<idno type="ISSN">0092-8674</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1998">1998</date>
<biblScope unit="volume">95</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="105">105</biblScope>
<biblScope unit="page" to="113">113</biblScope>
</imprint>
<idno type="ISSN">0092-8674</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0092-8674</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Agarose</term>
<term>Assay</term>
<term>Bdmv</term>
<term>Bdmv fragment</term>
<term>Bean dwarf mosaic geminivirus</term>
<term>Bind</term>
<term>Bind dsdna</term>
<term>Bind ssdna</term>
<term>Binding buffer</term>
<term>Binding properties</term>
<term>Binding studies</term>
<term>Bipartite</term>
<term>Bipartite geminiviruses</term>
<term>Circular forms</term>
<term>Circular ssdna</term>
<term>Coli proteins</term>
<term>Common region</term>
<term>Direct interaction</term>
<term>Dna</term>
<term>Dsdna</term>
<term>Final volume</term>
<term>Geminivirus</term>
<term>Geminiviruses</term>
<term>Genome</term>
<term>Genome size</term>
<term>Genomic</term>
<term>Genomic size</term>
<term>Gilbertson</term>
<term>Linear dsdna</term>
<term>Linear dsdna ladder</term>
<term>Linear forms</term>
<term>Lucas</term>
<term>Microinjection studies</term>
<term>Mobility</term>
<term>Mosaic</term>
<term>Movement protein</term>
<term>Movement proteins</term>
<term>Nondenaturing</term>
<term>Nondenaturing agarose</term>
<term>Noueiry</term>
<term>Nucleic</term>
<term>Nucleic acids</term>
<term>Pbda1</term>
<term>Plant cell</term>
<term>Plasmodesma</term>
<term>Plasmodesmal boundaries</term>
<term>Protein</term>
<term>Renatured</term>
<term>Rojas</term>
<term>Room temperature</term>
<term>Sequence specificity</term>
<term>Southern blot hybridization analysis</term>
<term>Ssdna</term>
<term>Supercoiled</term>
<term>Supercoiled form</term>
<term>Systemic infection</term>
<term>Viral</term>
<term>Viral movement</term>
<term>Virol</term>
<term>Virology</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Plant viral movement proteins mediate the cell-to-cell movement of nucleic acids. This involves either a direct interaction between the viral movement protein and the nucleic acid or an indirect interaction involving host factors. The bipartite geminiviruses possess two movement proteins, BV1 and BC1, that coordinate movement of viral DNA across nuclear and plasmodesmal boundaries, respectively. Here, we demonstrate that both BV1 and BC1 interact directly with DNA and, in addition, that they have the unique property to recognize DNA on the basis of form and size rather than sequence. This is a novel feature for plant virus movement proteins and raises the possibility that BV1 and BC1 may be determinants of genome size in the bipartite geminiviruses.</div>
</front>
</TEI>
<istex><corpusName>elsevier</corpusName>
<keywords><teeft><json:string>dsdna</json:string>
<json:string>ssdna</json:string>
<json:string>bdmv</json:string>
<json:string>pbda1</json:string>
<json:string>geminivirus</json:string>
<json:string>viral</json:string>
<json:string>genome</json:string>
<json:string>gilbertson</json:string>
<json:string>supercoiled</json:string>
<json:string>noueiry</json:string>
<json:string>geminiviruses</json:string>
<json:string>agarose</json:string>
<json:string>bipartite</json:string>
<json:string>plasmodesma</json:string>
<json:string>binding properties</json:string>
<json:string>dna</json:string>
<json:string>rojas</json:string>
<json:string>assay</json:string>
<json:string>nondenaturing agarose</json:string>
<json:string>virol</json:string>
<json:string>renatured</json:string>
<json:string>nondenaturing</json:string>
<json:string>genomic</json:string>
<json:string>bipartite geminiviruses</json:string>
<json:string>nucleic acids</json:string>
<json:string>binding buffer</json:string>
<json:string>linear forms</json:string>
<json:string>virology</json:string>
<json:string>plant cell</json:string>
<json:string>final volume</json:string>
<json:string>genome size</json:string>
<json:string>nucleic</json:string>
<json:string>movement protein</json:string>
<json:string>viral movement</json:string>
<json:string>bean dwarf mosaic geminivirus</json:string>
<json:string>systemic infection</json:string>
<json:string>common region</json:string>
<json:string>bdmv fragment</json:string>
<json:string>circular ssdna</json:string>
<json:string>bind</json:string>
<json:string>movement proteins</json:string>
<json:string>room temperature</json:string>
<json:string>plasmodesmal boundaries</json:string>
<json:string>supercoiled form</json:string>
<json:string>direct interaction</json:string>
<json:string>bind dsdna</json:string>
<json:string>genomic size</json:string>
<json:string>coli proteins</json:string>
<json:string>sequence specificity</json:string>
<json:string>linear dsdna ladder</json:string>
<json:string>circular forms</json:string>
<json:string>linear dsdna</json:string>
<json:string>bind ssdna</json:string>
<json:string>microinjection studies</json:string>
<json:string>southern blot hybridization analysis</json:string>
<json:string>binding studies</json:string>
<json:string>protein</json:string>
<json:string>lucas</json:string>
<json:string>mosaic</json:string>
<json:string>mobility</json:string>
</teeft>
</keywords>
<author><json:item><name>Maria R Rojas</name>
<affiliations><json:string>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA</json:string>
</affiliations>
</json:item>
<json:item><name>Amine O Noueiry</name>
<affiliations><json:string>Department of Plant Pathology, University of California, Davis, Davis, California 95616, USA</json:string>
<json:string>§ Present address: Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin 53706.</json:string>
</affiliations>
</json:item>
<json:item><name>William J Lucas</name>
<affiliations><json:string>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA</json:string>
</affiliations>
</json:item>
<json:item><name>Robert L Gilbertson</name>
<affiliations><json:string>Department of Plant Pathology, University of California, Davis, Davis, California 95616, USA</json:string>
<json:string>Correspondence: Robert L. Gilbertson, 530 752 3163 (phone), 530 752 5674 (fax)</json:string>
<json:string>‡ To whom correspondence should be addressed (e-mail: rlgilbertson@ucdavis.edu).</json:string>
<json:string>E-mail: rlgilbertson@ucdavis.edu</json:string>
</affiliations>
</json:item>
</author>
<arkIstex>ark:/67375/6H6-9NJW2TSN-8</arkIstex>
<language><json:string>eng</json:string>
</language>
<originalGenre><json:string>Full-length article</json:string>
</originalGenre>
<abstract>Abstract: Plant viral movement proteins mediate the cell-to-cell movement of nucleic acids. This involves either a direct interaction between the viral movement protein and the nucleic acid or an indirect interaction involving host factors. The bipartite geminiviruses possess two movement proteins, BV1 and BC1, that coordinate movement of viral DNA across nuclear and plasmodesmal boundaries, respectively. Here, we demonstrate that both BV1 and BC1 interact directly with DNA and, in addition, that they have the unique property to recognize DNA on the basis of form and size rather than sequence. This is a novel feature for plant virus movement proteins and raises the possibility that BV1 and BC1 may be determinants of genome size in the bipartite geminiviruses.</abstract>
<qualityIndicators><score>8.416</score>
<pdfWordCount>6844</pdfWordCount>
<pdfCharCount>40400</pdfCharCount>
<pdfVersion>1.3</pdfVersion>
<pdfPageCount>9</pdfPageCount>
<pdfPageSize>612 x 792 pts (letter)</pdfPageSize>
<refBibsNative>true</refBibsNative>
<abstractWordCount>118</abstractWordCount>
<abstractCharCount>769</abstractCharCount>
<keywordCount>0</keywordCount>
</qualityIndicators>
<title>Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner</title>
<pmid><json:string>9778251</json:string>
</pmid>
<pii><json:string>S0092-8674(00)81786-9</json:string>
</pii>
<genre><json:string>research-article</json:string>
</genre>
<host><title>Cell</title>
<language><json:string>unknown</json:string>
</language>
<publicationDate>1998</publicationDate>
<issn><json:string>0092-8674</json:string>
</issn>
<pii><json:string>S0092-8674(00)X0027-X</json:string>
</pii>
<volume>95</volume>
<issue>1</issue>
<pages><first>105</first>
<last>113</last>
</pages>
<genre><json:string>journal</json:string>
</genre>
</host>
<namedEntities><unitex><date><json:string>1998</json:string>
</date>
<geogName></geogName>
<orgName><json:string>Department of Agriculture National Research Initiative Competitive Grants Program</json:string>
<json:string>Department of Plant Pathology University of California, Davis Davis, California</json:string>
<json:string>Alpha Innotech Corporation</json:string>
<json:string>Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin</json:string>
<json:string>National Science Foundation</json:string>
<json:string>IBN</json:string>
</orgName>
<orgName_funder><json:string>National Science Foundation</json:string>
<json:string>IBN</json:string>
</orgName_funder>
<orgName_provider></orgName_provider>
<persName><json:string>R. Salati</json:string>
<json:string>Jan Monzer</json:string>
<json:string>B. Xoconostle-Cazares</json:string>
<json:string>M. Hou</json:string>
<json:string>Robert L. Gilbertson</json:string>
<json:string>Results</json:string>
<json:string>C. Kao</json:string>
<json:string>William J. Lucas</json:string>
<json:string>J. Gen</json:string>
<json:string>B. Ding</json:string>
<json:string>Viral Clones</json:string>
<json:string>Amine O. Noueiry</json:string>
<json:string>La Jolla</json:string>
<json:string>M. R. Sudarshana</json:string>
<json:string>Y. Lee</json:string>
</persName>
<placeName><json:string>San Leandro</json:string>
<json:string>CA</json:string>
</placeName>
<ref_url></ref_url>
<ref_bibl><json:string>Paplomatas et al., 1994</json:string>
<json:string>Maia et al., 1996</json:string>
<json:string>Ding et al., 1995</json:string>
<json:string>Azzam et al., 1994</json:string>
<json:string>Rojas et al., 1993</json:string>
<json:string>Ward et al., 1997</json:string>
<json:string>Schaffer et al., 1995</json:string>
<json:string>Pascal et al., 1994</json:string>
<json:string>Li and Palukaitis, 1996</json:string>
<json:string>Fujiwara et al., 1993</json:string>
<json:string>Giesman-Cookmeyer and Lommel, 1993</json:string>
<json:string>Hidayat et al., 1993</json:string>
<json:string>Brough et al., 1988</json:string>
<json:string>Gilbertson et al., 1991</json:string>
<json:string>Carrington et al, 1996</json:string>
<json:string>Rojas et al., 1997</json:string>
<json:string>Osman et al., 1992</json:string>
<json:string>Klinkenberg et al., 1989</json:string>
<json:string>Etessami et al., 1989</json:string>
<json:string>Sanderfoot and Lazarowitz, 1995</json:string>
<json:string>Carrington et al., 1996</json:string>
<json:string>Lucas and Gilbertson, 1994</json:string>
<json:string>Ghoshroy et al., 1997</json:string>
<json:string>McLean et al., 1997</json:string>
<json:string>Liu et al., 1997</json:string>
<json:string>Wang et al., 1996</json:string>
<json:string>Noueiry et al., 1994</json:string>
<json:string>Etessami et al., 1988</json:string>
<json:string>Gilbertson and Lucas, 1996</json:string>
<json:string>Ingham et al., 1995</json:string>
<json:string>Sudarshana et al., 1998</json:string>
<json:string>Qu and Morris, 1997</json:string>
<json:string>Stanley and Townsend, 1985</json:string>
<json:string>Citovsky et al., 1990</json:string>
<json:string>Timmermans et al., 1994</json:string>
<json:string>Elmer and Rogers, 1990</json:string>
<json:string>Brown et al., 1995</json:string>
<json:string>Hoefert, 1987</json:string>
<json:string>Deom et al., 1992</json:string>
<json:string>Hou et al., 1998</json:string>
</ref_bibl>
<bibl></bibl>
</unitex>
</namedEntities>
<ark><json:string>ark:/67375/6H6-9NJW2TSN-8</json:string>
</ark>
<categories><wos><json:string>1 - science</json:string>
<json:string>2 - cell biology</json:string>
<json:string>2 - biochemistry & molecular biology</json:string>
</wos>
<scienceMetrix><json:string>1 - health sciences</json:string>
<json:string>2 - biomedical research</json:string>
<json:string>3 - developmental biology</json:string>
</scienceMetrix>
<scopus><json:string>1 - Life Sciences</json:string>
<json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string>
<json:string>3 - General Biochemistry, Genetics and Molecular Biology</json:string>
</scopus>
<inist><json:string>1 - sciences appliquees, technologies et medecines</json:string>
<json:string>2 - sciences biologiques et medicales</json:string>
<json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string>
<json:string>4 - phytopathologie. zoologie agricole. protection des cultures et des forets</json:string>
</inist>
</categories>
<publicationDate>1998</publicationDate>
<copyrightDate>1998</copyrightDate>
<doi><json:string>10.1016/S0092-8674(00)81786-9</json:string>
</doi>
<id>0BC8612A0715E465962B785D74463947AAA69932</id>
<score>1</score>
<fulltext><json:item><extension>pdf</extension>
<original>true</original>
<mimetype>application/pdf</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-9NJW2TSN-8/fulltext.pdf</uri>
</json:item>
<json:item><extension>zip</extension>
<original>false</original>
<mimetype>application/zip</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-9NJW2TSN-8/bundle.zip</uri>
</json:item>
<istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-9NJW2TSN-8/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner</title>
</titleStmt>
<publicationStmt><authority>ISTEX</authority>
<publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher>
<availability><licence><p>©1998 Cell Press</p>
</licence>
<p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p>
</availability>
<date>1998</date>
</publicationStmt>
<notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note>
<note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note>
<note type="content">Section title: Article</note>
<note type="content">Figure 1: Expression of BDMV BV1 and BC1 Proteins and Analysis of Their In Vitro DNA Binding Properties (A) SDS-PAGE analysis showing E. coli-expressed BV1 and BC1 proteins purified by Ni-NTA column chromatography and renaturation. (B) Gel mobility-shift assays showing that the purified BV1 and BC1 fusion proteins bind dsDNA. Controls are the recombinant plasmid pBDA1 alone (lane 1), mixed with column-purified total renatured E. coli proteins (lane 2), or mixed with bovine serum albumin (lane 3). pBDA1 mixed with 1 μg of column-purified BV1 or BC1 protein before (lanes 4 and 8, respectively) or after (lanes 5 and 9, respectively) renaturation. Renatured BV1 or BC1 protein mixed with 0.1% SDS and then added to pBDA1 (lanes 6 and 10, respectively). E. coli–expressed BV1 or BC1 protein, lacking the N-terminal histidine tag (i.e., nonfusion proteins) and purified by electroelution, mixed with pBDA1 (lanes 7 and 11, respectively). All reactions were conducted with 50 ng of pBDA1 and 1 μg each of BV1 or BC1 protein in a final volume of 20 μl of binding buffer. Mixtures were analyzed in 0.7% nondenaturing agarose gels in TAE buffer.</note>
<note type="content">Figure 2: Kinetics of BDMV BV1 and BC1 Binding to Circular and Linear Forms of dsDNA (A) Circular or linear forms of pBDA1 mixed with increasing amounts (0.5–2.0 μg) of BV1 protein. (B) Circular or linear forms of pBDA1 mixed with increasing amounts (0.5–4.0 μg) of BC1 protein. (C) Circular forms of pSP72 mixed with increasing amounts (0.5–2.0 μg) of BV1 protein. The BRL supercoiled (sc) DNA ladder (0.5 μg) mixed with 1.0 or 2.0 μg of BV1 protein. As a control, pBDA1 was mixed with 1 μg of BV1 protein. (D) Circular forms of pSP72 mixed with increasing amounts (0.5–4.0 μg) of BC1 protein. The BRL sc DNA ladder (0.5 μg) mixed with 2.0 or 4.0 μg of BC1 protein. As a control, pBDA1 was mixed with 2 μg of BC1 protein. (E) Linear 1.3 kb BDMV DNA-A fragment (containing the common region) and linear double-stranded forms of pSP72 (∼2.4 kb), the BDMV DNA-B component (∼2.6 kb), or pKS (∼2.9 kb) mixed with 1.0 or 2.0 μg of BV1 protein. (F) Linear 1.3 kb BDMV DNA-A fragment (containing the common region) and linear double-stranded forms of pSP72, the BDMV DNA-A component, or pKS mixed with 2.0 or 4.0 μg of BC1 protein. All reactions were conducted with 50 ng of DNA (except for pSP72, in which 65 ng were used) in a final volume of 20 μl of binding buffer. Mixtures were analyzed in 0.7% nondenaturing agarose gels in TAE buffer. DNA forms are identified as follows: open circular (oc), relaxed circular (rc), supercoiled (sc), and linear (l).</note>
<note type="content">Figure 3: The BDMV BV1 and BC1 Proteins Recognize Linear DNAs on the Basis of Size (A) Increasing amounts of BV1 (0.5–2.0 μg) mixed with 0.5 μg of the BRL 1 kb linear dsDNA ladder. (B) Increasing amounts of BC1 (0.5–2.0 μg) mixed with 0.5 μg of the BRL 1 kb linear dsDNA ladder. All reactions were conducted in a final volume of 20 μl of binding buffer, and mixtures were analyzed in 0.7% nondenaturing agarose gels in TAE buffer.</note>
<note type="content">Figure 4: Kinetics of BDMV BV1 and BC1 Protein Binding to Circular ssDNA (A) BV1 protein (0.5 or 1.0 μg) mixed with increasing amounts of M13 ssDNA. (B) BC1 protein (2.0 or 4.0 μg) mixed with increasing amounts of M13 ssDNA. Lane 1 in (A) and (B) is the BRL 1 kb linear dsDNA ladder, and lane 2 is 100 ng of M13 ssDNA alone. All reactions were conducted in a final volume of 20 μl of binding buffer, and mixtures were analyzed in 0.7% nondenaturing agarose gels in TAE buffer.</note>
<note type="content">Figure 5: Analysis of the Affinity of BDMV BV1 Protein for Binding ssDNA and dsDNA (A) BV1 protein (1 μg) mixed with 32P-labeled pBDA1 (50 ng) and kept at 22°C for 15 min. Increasing amounts of M13 ssDNA (25–200 ng) were then added to individual BV1 + pBDA1 mixtures and kept at 22°C for a further 15 min. Mixtures were then analyzed in 0.7% nondenaturing agarose gels in TAE buffer. DNA forms are shown as follows: open circular (oc), relaxed circular (rc), linear (l), and single-stranded (ss). Results shown as an ethidium bromide–stained gel. (B) Southern blot analysis of agarose gel presented in (A) showing migration of 32P-labeled pBDA1. Controls in (A) and (B): 32P-labeled pBDA1 (50 ng) and M13 ssDNA (50 or 100 ng) were run alone ([−], lanes 1, 3, and 5, respectively) or mixed with 1 μg of BV1 ([+], lanes 2, 4, and 6, respectively). As an additional control, 32P-labeled pBDA1 (50 ng) and M13 ssDNA (50 ng) were mixed and kept at 22°C for 15 min (lane 7).</note>
<note type="content">Figure 6: Analysis of the Affinity of BDMV BC1 Protein for Binding ssDNA and dsDNA BC1 protein (2 μg) was mixed with ssDNA (50 ng) and kept at 22°C for 15 min. Increasing amounts of pBDA1 (25–200 ng) were then added to individual BC1 + ssDNA mixtures and kept at 22°C for another 15 min. As controls, pBDA1 (50 ng) and M13 ssDNA (50 ng) were run alone ([−], lanes 1 and 3, respectively) or mixed with 2 μg of BC1 ([+], lanes 2 and 4, respectively). As an additional control, pBDA1 (50 ng) and M13 ssDNA (50 ng) were run alone (lane 5). All reactions were conducted in a final volume of 20 μl of binding buffer, and mixtures were analyzed in 0.7% nondenaturing agarose gels in TAE buffer. DNA forms are shown as follows: open circular (oc), supercoiled (sc), and single-stranded (ss).</note>
<note type="content">Figure 7: Binding of BV1 and BC1 Proteins to Viral DNA Forms Extracted from BDMV-Infected Tobacco Suspension Culture Cells (A) BV1 (1.0 or 2.0 μg), BC1 (2.0 or 4.0 μg), or a combination of BV1 and BC1 mixed with DNA extracted from BDMV-infected N. tabacum suspension culture cells. As a control, pBDA1 (50 ng) was mixed with 2 μg of BV1 (lane 3) or BC1 (lane 4). Mixtures were analyzed in 0.7% nondenaturing agarose gels in TAE buffer. Results shown as an ethidium bromide–stained gel. (B) Southern blot hybridization analysis of agarose gel presented in (A) showing migration of the BDMV DNA forms and pBDA1. Blot was probed with 32P-labeled pBDA1 under conditions of low stringency. DNA forms are shown as follows: open circular (oc), linear (l), single-stranded (ss), and supercoiled (sc).</note>
</notesStmt>
<sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner</title>
<author xml:id="author-0000"><persName><forename type="first">Maria R</forename>
<surname>Rojas</surname>
</persName>
<affiliation>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA</affiliation>
</author>
<author xml:id="author-0001"><persName><forename type="first">Amine O</forename>
<surname>Noueiry</surname>
</persName>
<affiliation>Department of Plant Pathology, University of California, Davis, Davis, California 95616, USA</affiliation>
<affiliation>§ Present address: Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin 53706.</affiliation>
</author>
<author xml:id="author-0002"><persName><forename type="first">William J</forename>
<surname>Lucas</surname>
</persName>
<affiliation>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA</affiliation>
</author>
<author xml:id="author-0003"><persName><forename type="first">Robert L</forename>
<surname>Gilbertson</surname>
</persName>
<email>rlgilbertson@ucdavis.edu</email>
<affiliation>Department of Plant Pathology, University of California, Davis, Davis, California 95616, USA</affiliation>
<affiliation>Correspondence: Robert L. Gilbertson, 530 752 3163 (phone), 530 752 5674 (fax)</affiliation>
<affiliation>‡ To whom correspondence should be addressed (e-mail: rlgilbertson@ucdavis.edu).</affiliation>
</author>
<idno type="istex">0BC8612A0715E465962B785D74463947AAA69932</idno>
<idno type="ark">ark:/67375/6H6-9NJW2TSN-8</idno>
<idno type="DOI">10.1016/S0092-8674(00)81786-9</idno>
<idno type="PII">S0092-8674(00)81786-9</idno>
</analytic>
<monogr><title level="j">Cell</title>
<title level="j" type="abbrev">CELL</title>
<idno type="pISSN">0092-8674</idno>
<idno type="PII">S0092-8674(00)X0027-X</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1998"></date>
<biblScope unit="volume">95</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="105">105</biblScope>
<biblScope unit="page" to="113">113</biblScope>
</imprint>
</monogr>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><creation><date>1998</date>
</creation>
<langUsage><language ident="en">en</language>
</langUsage>
<abstract xml:lang="en"><p>Abstract: Plant viral movement proteins mediate the cell-to-cell movement of nucleic acids. This involves either a direct interaction between the viral movement protein and the nucleic acid or an indirect interaction involving host factors. The bipartite geminiviruses possess two movement proteins, BV1 and BC1, that coordinate movement of viral DNA across nuclear and plasmodesmal boundaries, respectively. Here, we demonstrate that both BV1 and BC1 interact directly with DNA and, in addition, that they have the unique property to recognize DNA on the basis of form and size rather than sequence. This is a novel feature for plant virus movement proteins and raises the possibility that BV1 and BC1 may be determinants of genome size in the bipartite geminiviruses.</p>
</abstract>
</profileDesc>
<revisionDesc><change when="1998-08-12">Modified</change>
<change when="1998">Published</change>
</revisionDesc>
</teiHeader>
</istex:fulltextTEI>
<json:item><extension>txt</extension>
<original>false</original>
<mimetype>text/plain</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-9NJW2TSN-8/fulltext.txt</uri>
</json:item>
</fulltext>
<metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration>
<istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity>
<istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity>
<istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity>
<istex:entity SYSTEM="gr4" NDATA="IMAGE" name="gr4"></istex:entity>
<istex:entity SYSTEM="gr5" NDATA="IMAGE" name="gr5"></istex:entity>
<istex:entity SYSTEM="gr6" NDATA="IMAGE" name="gr6"></istex:entity>
<istex:entity SYSTEM="gr7" NDATA="IMAGE" name="gr7"></istex:entity>
</istex:docType>
<istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>CELL</jid>
<aid>5147</aid>
<ce:pii>S0092-8674(00)81786-9</ce:pii>
<ce:doi>10.1016/S0092-8674(00)81786-9</ce:doi>
<ce:copyright type="other" year="1998">Cell Press</ce:copyright>
</item-info>
<head><ce:dochead><ce:textfn>Article</ce:textfn>
</ce:dochead>
<ce:title>Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner</ce:title>
<ce:author-group><ce:author><ce:given-name>Maria R</ce:given-name>
<ce:surname>Rojas</ce:surname>
<ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Amine O</ce:given-name>
<ce:surname>Noueiry</ce:surname>
<ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup>
</ce:cross-ref>
<ce:cross-ref refid="FN2">§</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>William J</ce:given-name>
<ce:surname>Lucas</ce:surname>
<ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Robert L</ce:given-name>
<ce:surname>Gilbertson</ce:surname>
<ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup>
</ce:cross-ref>
<ce:cross-ref refid="FN1">‡</ce:cross-ref>
<ce:cross-ref refid="COR1">*</ce:cross-ref>
<ce:e-address>rlgilbertson@ucdavis.edu</ce:e-address>
</ce:author>
<ce:affiliation id="AFF1"><ce:label>1</ce:label>
<ce:textfn>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA</ce:textfn>
</ce:affiliation>
<ce:affiliation id="AFF2"><ce:label>2</ce:label>
<ce:textfn>Department of Plant Pathology, University of California, Davis, Davis, California 95616, USA</ce:textfn>
</ce:affiliation>
<ce:correspondence id="COR1"><ce:label>*</ce:label>
<ce:text>Correspondence: Robert L. Gilbertson, 530 752 3163 (phone), 530 752 5674 (fax)</ce:text>
</ce:correspondence>
</ce:author-group>
<ce:date-received day="26" month="5" year="1998"></ce:date-received>
<ce:date-revised day="12" month="8" year="1998"></ce:date-revised>
<ce:abstract><ce:section-title>Abstract</ce:section-title>
<ce:abstract-sec><ce:simple-para>Plant viral movement proteins mediate the cell-to-cell movement of nucleic acids. This involves either a direct interaction between the viral movement protein and the nucleic acid or an indirect interaction involving host factors. The bipartite geminiviruses possess two movement proteins, BV1 and BC1, that coordinate movement of viral DNA across nuclear and plasmodesmal boundaries, respectively. Here, we demonstrate that both BV1 and BC1 interact directly with DNA and, in addition, that they have the unique property to recognize DNA on the basis of form and size rather than sequence. This is a novel feature for plant virus movement proteins and raises the possibility that BV1 and BC1 may be determinants of genome size in the bipartite geminiviruses.</ce:simple-para>
</ce:abstract-sec>
</ce:abstract>
</head>
</converted-article>
</istex:document>
</istex:metadataXml>
<mods version="3.6"><titleInfo lang="en"><title>Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner</title>
</titleInfo>
<titleInfo type="alternative" lang="en" contentType="CDATA"><title>Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner</title>
</titleInfo>
<name type="personal"><namePart type="given">Maria R</namePart>
<namePart type="family">Rojas</namePart>
<affiliation>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Amine O</namePart>
<namePart type="family">Noueiry</namePart>
<affiliation>Department of Plant Pathology, University of California, Davis, Davis, California 95616, USA</affiliation>
<affiliation>§ Present address: Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin 53706.</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">William J</namePart>
<namePart type="family">Lucas</namePart>
<affiliation>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Robert L</namePart>
<namePart type="family">Gilbertson</namePart>
<affiliation>Department of Plant Pathology, University of California, Davis, Davis, California 95616, USA</affiliation>
<affiliation>Correspondence: Robert L. Gilbertson, 530 752 3163 (phone), 530 752 5674 (fax)</affiliation>
<affiliation>‡ To whom correspondence should be addressed (e-mail: rlgilbertson@ucdavis.edu).</affiliation>
<affiliation>E-mail: rlgilbertson@ucdavis.edu</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
<genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre>
<originInfo><publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1998</dateIssued>
<dateModified encoding="w3cdtf">1998-08-12</dateModified>
<copyrightDate encoding="w3cdtf">1998</copyrightDate>
</originInfo>
<language><languageTerm type="code" authority="iso639-2b">eng</languageTerm>
<languageTerm type="code" authority="rfc3066">en</languageTerm>
</language>
<abstract lang="en">Abstract: Plant viral movement proteins mediate the cell-to-cell movement of nucleic acids. This involves either a direct interaction between the viral movement protein and the nucleic acid or an indirect interaction involving host factors. The bipartite geminiviruses possess two movement proteins, BV1 and BC1, that coordinate movement of viral DNA across nuclear and plasmodesmal boundaries, respectively. Here, we demonstrate that both BV1 and BC1 interact directly with DNA and, in addition, that they have the unique property to recognize DNA on the basis of form and size rather than sequence. This is a novel feature for plant virus movement proteins and raises the possibility that BV1 and BC1 may be determinants of genome size in the bipartite geminiviruses.</abstract>
<note type="content">Section title: Article</note>
<note type="content">Figure 1: Expression of BDMV BV1 and BC1 Proteins and Analysis of Their In Vitro DNA Binding Properties (A) SDS-PAGE analysis showing E. coli-expressed BV1 and BC1 proteins purified by Ni-NTA column chromatography and renaturation. (B) Gel mobility-shift assays showing that the purified BV1 and BC1 fusion proteins bind dsDNA. Controls are the recombinant plasmid pBDA1 alone (lane 1), mixed with column-purified total renatured E. coli proteins (lane 2), or mixed with bovine serum albumin (lane 3). pBDA1 mixed with 1 μg of column-purified BV1 or BC1 protein before (lanes 4 and 8, respectively) or after (lanes 5 and 9, respectively) renaturation. Renatured BV1 or BC1 protein mixed with 0.1% SDS and then added to pBDA1 (lanes 6 and 10, respectively). E. coli–expressed BV1 or BC1 protein, lacking the N-terminal histidine tag (i.e., nonfusion proteins) and purified by electroelution, mixed with pBDA1 (lanes 7 and 11, respectively). All reactions were conducted with 50 ng of pBDA1 and 1 μg each of BV1 or BC1 protein in a final volume of 20 μl of binding buffer. Mixtures were analyzed in 0.7% nondenaturing agarose gels in TAE buffer.</note>
<note type="content">Figure 2: Kinetics of BDMV BV1 and BC1 Binding to Circular and Linear Forms of dsDNA (A) Circular or linear forms of pBDA1 mixed with increasing amounts (0.5–2.0 μg) of BV1 protein. (B) Circular or linear forms of pBDA1 mixed with increasing amounts (0.5–4.0 μg) of BC1 protein. (C) Circular forms of pSP72 mixed with increasing amounts (0.5–2.0 μg) of BV1 protein. The BRL supercoiled (sc) DNA ladder (0.5 μg) mixed with 1.0 or 2.0 μg of BV1 protein. As a control, pBDA1 was mixed with 1 μg of BV1 protein. (D) Circular forms of pSP72 mixed with increasing amounts (0.5–4.0 μg) of BC1 protein. The BRL sc DNA ladder (0.5 μg) mixed with 2.0 or 4.0 μg of BC1 protein. As a control, pBDA1 was mixed with 2 μg of BC1 protein. (E) Linear 1.3 kb BDMV DNA-A fragment (containing the common region) and linear double-stranded forms of pSP72 (∼2.4 kb), the BDMV DNA-B component (∼2.6 kb), or pKS (∼2.9 kb) mixed with 1.0 or 2.0 μg of BV1 protein. (F) Linear 1.3 kb BDMV DNA-A fragment (containing the common region) and linear double-stranded forms of pSP72, the BDMV DNA-A component, or pKS mixed with 2.0 or 4.0 μg of BC1 protein. All reactions were conducted with 50 ng of DNA (except for pSP72, in which 65 ng were used) in a final volume of 20 μl of binding buffer. Mixtures were analyzed in 0.7% nondenaturing agarose gels in TAE buffer. DNA forms are identified as follows: open circular (oc), relaxed circular (rc), supercoiled (sc), and linear (l).</note>
<note type="content">Figure 3: The BDMV BV1 and BC1 Proteins Recognize Linear DNAs on the Basis of Size (A) Increasing amounts of BV1 (0.5–2.0 μg) mixed with 0.5 μg of the BRL 1 kb linear dsDNA ladder. (B) Increasing amounts of BC1 (0.5–2.0 μg) mixed with 0.5 μg of the BRL 1 kb linear dsDNA ladder. All reactions were conducted in a final volume of 20 μl of binding buffer, and mixtures were analyzed in 0.7% nondenaturing agarose gels in TAE buffer.</note>
<note type="content">Figure 4: Kinetics of BDMV BV1 and BC1 Protein Binding to Circular ssDNA (A) BV1 protein (0.5 or 1.0 μg) mixed with increasing amounts of M13 ssDNA. (B) BC1 protein (2.0 or 4.0 μg) mixed with increasing amounts of M13 ssDNA. Lane 1 in (A) and (B) is the BRL 1 kb linear dsDNA ladder, and lane 2 is 100 ng of M13 ssDNA alone. All reactions were conducted in a final volume of 20 μl of binding buffer, and mixtures were analyzed in 0.7% nondenaturing agarose gels in TAE buffer.</note>
<note type="content">Figure 5: Analysis of the Affinity of BDMV BV1 Protein for Binding ssDNA and dsDNA (A) BV1 protein (1 μg) mixed with 32P-labeled pBDA1 (50 ng) and kept at 22°C for 15 min. Increasing amounts of M13 ssDNA (25–200 ng) were then added to individual BV1 + pBDA1 mixtures and kept at 22°C for a further 15 min. Mixtures were then analyzed in 0.7% nondenaturing agarose gels in TAE buffer. DNA forms are shown as follows: open circular (oc), relaxed circular (rc), linear (l), and single-stranded (ss). Results shown as an ethidium bromide–stained gel. (B) Southern blot analysis of agarose gel presented in (A) showing migration of 32P-labeled pBDA1. Controls in (A) and (B): 32P-labeled pBDA1 (50 ng) and M13 ssDNA (50 or 100 ng) were run alone ([−], lanes 1, 3, and 5, respectively) or mixed with 1 μg of BV1 ([+], lanes 2, 4, and 6, respectively). As an additional control, 32P-labeled pBDA1 (50 ng) and M13 ssDNA (50 ng) were mixed and kept at 22°C for 15 min (lane 7).</note>
<note type="content">Figure 6: Analysis of the Affinity of BDMV BC1 Protein for Binding ssDNA and dsDNA BC1 protein (2 μg) was mixed with ssDNA (50 ng) and kept at 22°C for 15 min. Increasing amounts of pBDA1 (25–200 ng) were then added to individual BC1 + ssDNA mixtures and kept at 22°C for another 15 min. As controls, pBDA1 (50 ng) and M13 ssDNA (50 ng) were run alone ([−], lanes 1 and 3, respectively) or mixed with 2 μg of BC1 ([+], lanes 2 and 4, respectively). As an additional control, pBDA1 (50 ng) and M13 ssDNA (50 ng) were run alone (lane 5). All reactions were conducted in a final volume of 20 μl of binding buffer, and mixtures were analyzed in 0.7% nondenaturing agarose gels in TAE buffer. DNA forms are shown as follows: open circular (oc), supercoiled (sc), and single-stranded (ss).</note>
<note type="content">Figure 7: Binding of BV1 and BC1 Proteins to Viral DNA Forms Extracted from BDMV-Infected Tobacco Suspension Culture Cells (A) BV1 (1.0 or 2.0 μg), BC1 (2.0 or 4.0 μg), or a combination of BV1 and BC1 mixed with DNA extracted from BDMV-infected N. tabacum suspension culture cells. As a control, pBDA1 (50 ng) was mixed with 2 μg of BV1 (lane 3) or BC1 (lane 4). Mixtures were analyzed in 0.7% nondenaturing agarose gels in TAE buffer. Results shown as an ethidium bromide–stained gel. (B) Southern blot hybridization analysis of agarose gel presented in (A) showing migration of the BDMV DNA forms and pBDA1. Blot was probed with 32P-labeled pBDA1 under conditions of low stringency. DNA forms are shown as follows: open circular (oc), linear (l), single-stranded (ss), and supercoiled (sc).</note>
<relatedItem type="host"><titleInfo><title>Cell</title>
</titleInfo>
<titleInfo type="abbreviated"><title>CELL</title>
</titleInfo>
<genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre>
<originInfo><publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1998</dateIssued>
</originInfo>
<identifier type="ISSN">0092-8674</identifier>
<identifier type="PII">S0092-8674(00)X0027-X</identifier>
<part><date>1998</date>
<detail type="volume"><number>95</number>
<caption>vol.</caption>
</detail>
<detail type="issue"><number>1</number>
<caption>no.</caption>
</detail>
<extent unit="issue-pages"><start>1</start>
<end>146</end>
</extent>
<extent unit="pages"><start>105</start>
<end>113</end>
</extent>
</part>
</relatedItem>
<identifier type="istex">0BC8612A0715E465962B785D74463947AAA69932</identifier>
<identifier type="ark">ark:/67375/6H6-9NJW2TSN-8</identifier>
<identifier type="DOI">10.1016/S0092-8674(00)81786-9</identifier>
<identifier type="PII">S0092-8674(00)81786-9</identifier>
<accessCondition type="use and reproduction" contentType="copyright">©1998 Cell Press</accessCondition>
<recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource>
<recordOrigin>Cell Press, ©1998</recordOrigin>
</recordInfo>
</mods>
<json:item><extension>json</extension>
<original>false</original>
<mimetype>application/json</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-9NJW2TSN-8/record.json</uri>
</json:item>
</metadata>
<serie></serie>
</istex>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E14 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001E14 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Istex
   |étape=   Corpus
   |type=    RBID
   |clé=     ISTEX:0BC8612A0715E465962B785D74463947AAA69932
   |texte=   Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner
}}

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner

Bean Dwarf Mosaic Geminivirus Movement Proteins Recognize DNA in a Form- and Size-Specific Manner

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri