Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs
Identifieur interne : 001E13 ( Istex/Corpus ); précédent : 001E12; suivant : 001E14Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs
Auteurs : Emanuelle Pascolo ; Denis Hudrisier ; Brian Sproat ; Nguyen T. Thuong ; Jean-Jacques ToulmeSource :
- BBA - Gene Structure and Expression [ 0167-4781 ] ; 1994.
English descriptors
- Teeft :
- Acad, Acta, Amazonensis, Antisense, Antisense effect, Antisense oligonucleotide, Antisense oligonucleotides, Base pairs, Biochimica, Biophysica, Biophysica acta, Denaturing polyacrylamide, Duplex, Electrophoretic analysis, Gene expression, Hairpin, Hairpin structure, Helper oligonucleotide, Leishmania, Leishmania amazonensis, Leishmania mrnas, Mrna, Natl, Nucleic, Nucleic acids, Oligomer, Oligomers, Oligonucleotide, Oligonucleotides, Pascolo, Polyacrylamide, Previous study, Proc, Protein synthesis, Psoralen, Psoralen derivatives, Rabbit reticulocyte lysate, Reticulocyte, Rnase, Simultaneous addition, Thuong, Toulm, Toulm6, Wheat germ.
Abstract
Abstract: We investigated the properties of two antisense oligonucleotides, 11αPso and 14TMP, 11 and 14 nucleotides long, respectively, and conjugated to psoralen derivatives. These oligonucleotides were complementary to the mini-exon sequence of Leishmania amazonensis. Upon ultraviolet (UV) irradiation these oligomers were selectively cross-linked to DNA or RNA target sequences, either 14 or 35 nucleotides long. The yield of photo-addition was much lower on the longer targets than on the shorter ones, due to the presence of a hairpin structure. The co-addition of a helper oligonucleotide, whose binding site, on the 35-mer, was adjacent to that of the psoralen-derivatized antisense oligomer, improved the cross-linking efficiency. We then determined the effect of 14TMP on in vitro translation of Leishmania mRNA in cell-free extracts. Non-irradiated antisense oligonucleotide/mRNA complexes reduced the protein synthesis in wheat germ extract but not in rabbit reticulocyte lysate. Conversely, UV irradiation induced a 14TMP-dependent reduction of translation in reticulocyte lysate whereas the inhibition was not improved in the wheat germ extract. These results are discussed with respect to the involvement of RNase-H in the oligonucleotide-mediated effect on protein synthesis.
Url:
DOI: 10.1016/0167-4781(94)90251-8
Links to Exploration step
ISTEX:3245568FFAB7443833309DB61736BFA3E1F3CD79Le document en format XML
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Fax: + 33 57 571015</mods:affiliation></affiliation><affiliation><mods:affiliation>Laboratoire de Biophysique Moléculaire, INSERM U386, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux cédex, France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:3245568FFAB7443833309DB61736BFA3E1F3CD79</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1016/0167-4781(94)90251-8</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-RHHJRVB1-G/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001E13</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001E13</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs</title><author><name sortKey="Pascolo, Emanuelle" sort="Pascolo, Emanuelle" uniqKey="Pascolo E" first="Emanuelle" last="Pascolo">Emanuelle Pascolo</name><affiliation><mods:affiliation>Laboratoire de Biophysique Moléculaire, INSERM U386, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux cédex, France</mods:affiliation></affiliation></author><author><name sortKey="Hudrisier, Denis" sort="Hudrisier, Denis" uniqKey="Hudrisier D" first="Denis" last="Hudrisier">Denis Hudrisier</name><affiliation><mods:affiliation>Sanofi Elf-Biorecherches, Labège Innopole, BP 137, 31676 Labège cédex, France</mods:affiliation></affiliation></author><author><name sortKey="Sproat, Brian" sort="Sproat, Brian" uniqKey="Sproat B" first="Brian" last="Sproat">Brian Sproat</name><affiliation><mods:affiliation>EMBL, Meyerhofstrasse 1, D-69012 Heidelberg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Thuong, Nguyen T" sort="Thuong, Nguyen T" uniqKey="Thuong N" first="Nguyen T." last="Thuong">Nguyen T. 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Fax: + 33 57 571015</mods:affiliation></affiliation><affiliation><mods:affiliation>Laboratoire de Biophysique Moléculaire, INSERM U386, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux cédex, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">BBA - Gene Structure and Expression</title><title level="j" type="abbrev">BBAEXP</title><idno type="ISSN">0167-4781</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">1219</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="98">98</biblScope><biblScope unit="page" to="106">106</biblScope></imprint><idno type="ISSN">0167-4781</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0167-4781</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Acta</term><term>Amazonensis</term><term>Antisense</term><term>Antisense effect</term><term>Antisense oligonucleotide</term><term>Antisense oligonucleotides</term><term>Base pairs</term><term>Biochimica</term><term>Biophysica</term><term>Biophysica acta</term><term>Denaturing polyacrylamide</term><term>Duplex</term><term>Electrophoretic analysis</term><term>Gene expression</term><term>Hairpin</term><term>Hairpin structure</term><term>Helper oligonucleotide</term><term>Leishmania</term><term>Leishmania amazonensis</term><term>Leishmania mrnas</term><term>Mrna</term><term>Natl</term><term>Nucleic</term><term>Nucleic acids</term><term>Oligomer</term><term>Oligomers</term><term>Oligonucleotide</term><term>Oligonucleotides</term><term>Pascolo</term><term>Polyacrylamide</term><term>Previous study</term><term>Proc</term><term>Protein synthesis</term><term>Psoralen</term><term>Psoralen derivatives</term><term>Rabbit reticulocyte lysate</term><term>Reticulocyte</term><term>Rnase</term><term>Simultaneous addition</term><term>Thuong</term><term>Toulm</term><term>Toulm6</term><term>Wheat germ</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We investigated the properties of two antisense oligonucleotides, 11αPso and 14TMP, 11 and 14 nucleotides long, respectively, and conjugated to psoralen derivatives. These oligonucleotides were complementary to the mini-exon sequence of Leishmania amazonensis. Upon ultraviolet (UV) irradiation these oligomers were selectively cross-linked to DNA or RNA target sequences, either 14 or 35 nucleotides long. The yield of photo-addition was much lower on the longer targets than on the shorter ones, due to the presence of a hairpin structure. The co-addition of a helper oligonucleotide, whose binding site, on the 35-mer, was adjacent to that of the psoralen-derivatized antisense oligomer, improved the cross-linking efficiency. We then determined the effect of 14TMP on in vitro translation of Leishmania mRNA in cell-free extracts. Non-irradiated antisense oligonucleotide/mRNA complexes reduced the protein synthesis in wheat germ extract but not in rabbit reticulocyte lysate. Conversely, UV irradiation induced a 14TMP-dependent reduction of translation in reticulocyte lysate whereas the inhibition was not improved in the wheat germ extract. 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Thuong</name><affiliations><json:string>Centre de Biophysique Moléculaire, CNRS, 45071 Orléans, France</json:string></affiliations></json:item><json:item><name>Jean-Jacques Toulme</name><affiliations><json:string>Corresponding author. Fax: + 33 57 571015</json:string><json:string>Laboratoire de Biophysique Moléculaire, INSERM U386, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux cédex, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Antisense oligonucleotide</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Cell-free translation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Alpha oligonucleotide</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Photosensitizer</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Hairpin structure</value></json:item></subject><arkIstex>ark:/67375/6H6-RHHJRVB1-G</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: We investigated the properties of two antisense oligonucleotides, 11αPso and 14TMP, 11 and 14 nucleotides long, respectively, and conjugated to psoralen derivatives. These oligonucleotides were complementary to the mini-exon sequence of Leishmania amazonensis. Upon ultraviolet (UV) irradiation these oligomers were selectively cross-linked to DNA or RNA target sequences, either 14 or 35 nucleotides long. The yield of photo-addition was much lower on the longer targets than on the shorter ones, due to the presence of a hairpin structure. The co-addition of a helper oligonucleotide, whose binding site, on the 35-mer, was adjacent to that of the psoralen-derivatized antisense oligomer, improved the cross-linking efficiency. We then determined the effect of 14TMP on in vitro translation of Leishmania mRNA in cell-free extracts. Non-irradiated antisense oligonucleotide/mRNA complexes reduced the protein synthesis in wheat germ extract but not in rabbit reticulocyte lysate. Conversely, UV irradiation induced a 14TMP-dependent reduction of translation in reticulocyte lysate whereas the inhibition was not improved in the wheat germ extract. 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Pascolo</json:string></persName><placeName><json:string>France</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>[4]</json:string><json:string>[10,11]</json:string><json:string>[23]</json:string><json:string>[29]</json:string><json:string>[3,43]</json:string><json:string>[2,3]</json:string><json:string>[32-35]</json:string><json:string>[44]</json:string><json:string>[20,21]</json:string><json:string>[8]</json:string><json:string>[39]</json:string><json:string>[1]</json:string><json:string>[32]</json:string><json:string>[38]</json:string><json:string>[46,47]</json:string><json:string>[31,42]</json:string><json:string>[31,45]</json:string><json:string>[5]</json:string><json:string>[48]</json:string><json:string>[6,7]</json:string><json:string>[41]</json:string><json:string>Verspieren et al. [12]</json:string><json:string>[25]</json:string><json:string>E. Pascolo et al.</json:string><json:string>[9]</json:string><json:string>[40]</json:string><json:string>E. Pascolo et aL</json:string><json:string>[13]</json:string><json:string>[19]</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-RHHJRVB1-G</json:string></ark><categories><wos></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - biochemistry & molecular biology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Genetics</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Biochemistry</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Biophysics</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Structural Biology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1994</publicationDate><copyrightDate>1994</copyrightDate><doi><json:string>10.1016/0167-4781(94)90251-8</json:string></doi><id>3245568FFAB7443833309DB61736BFA3E1F3CD79</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RHHJRVB1-G/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RHHJRVB1-G/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-RHHJRVB1-G/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1994</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Regular paper</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs</title><author xml:id="author-0000"><persName><forename type="first">Emanuelle</forename><surname>Pascolo</surname></persName><affiliation>Laboratoire de Biophysique Moléculaire, INSERM U386, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux cédex, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Denis</forename><surname>Hudrisier</surname></persName><affiliation>Sanofi Elf-Biorecherches, Labège Innopole, BP 137, 31676 Labège cédex, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Brian</forename><surname>Sproat</surname></persName><affiliation>EMBL, Meyerhofstrasse 1, D-69012 Heidelberg, Germany</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Nguyen T.</forename><surname>Thuong</surname></persName><affiliation>Centre de Biophysique Moléculaire, CNRS, 45071 Orléans, France</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Jean-Jacques</forename><surname>Toulme</surname></persName><affiliation>Corresponding author. Fax: + 33 57 571015</affiliation><affiliation>Laboratoire de Biophysique Moléculaire, INSERM U386, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux cédex, France</affiliation></author><idno type="istex">3245568FFAB7443833309DB61736BFA3E1F3CD79</idno><idno type="ark">ark:/67375/6H6-RHHJRVB1-G</idno><idno type="DOI">10.1016/0167-4781(94)90251-8</idno><idno type="PII">0167-4781(94)90251-8</idno></analytic><monogr><title level="j">BBA - Gene Structure and Expression</title><title level="j" type="abbrev">BBAEXP</title><idno type="pISSN">0167-4781</idno><idno type="PII">S0167-4781(00)X0203-5</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994"></date><biblScope unit="volume">1219</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="98">98</biblScope><biblScope unit="page" to="106">106</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1994</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: We investigated the properties of two antisense oligonucleotides, 11αPso and 14TMP, 11 and 14 nucleotides long, respectively, and conjugated to psoralen derivatives. These oligonucleotides were complementary to the mini-exon sequence of Leishmania amazonensis. Upon ultraviolet (UV) irradiation these oligomers were selectively cross-linked to DNA or RNA target sequences, either 14 or 35 nucleotides long. The yield of photo-addition was much lower on the longer targets than on the shorter ones, due to the presence of a hairpin structure. The co-addition of a helper oligonucleotide, whose binding site, on the 35-mer, was adjacent to that of the psoralen-derivatized antisense oligomer, improved the cross-linking efficiency. We then determined the effect of 14TMP on in vitro translation of Leishmania mRNA in cell-free extracts. Non-irradiated antisense oligonucleotide/mRNA complexes reduced the protein synthesis in wheat germ extract but not in rabbit reticulocyte lysate. Conversely, UV irradiation induced a 14TMP-dependent reduction of translation in reticulocyte lysate whereas the inhibition was not improved in the wheat germ extract. These results are discussed with respect to the involvement of RNase-H in the oligonucleotide-mediated effect on protein synthesis.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Antisense oligonucleotide</term></item><item><term>Cell-free translation</term></item><item><term>Alpha oligonucleotide</term></item><item><term>Photosensitizer</term></item><item><term>Hairpin structure</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1994">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RHHJRVB1-G/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>BBAEXP</jid><aid>94902518</aid><ce:pii>0167-4781(94)90251-8</ce:pii><ce:doi>10.1016/0167-4781(94)90251-8</ce:doi><ce:copyright type="unknown" year="1994"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular paper</ce:textfn></ce:dochead><ce:title>Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of <ce:italic>Leishmania</ce:italic> mRNAs</ce:title><ce:author-group><ce:author><ce:given-name>Emanuelle</ce:given-name><ce:surname>Pascolo</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Denis</ce:given-name><ce:surname>Hudrisier</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Brian</ce:given-name><ce:surname>Sproat</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Nguyen T.</ce:given-name><ce:surname>Thuong</ce:surname><ce:cross-ref refid="AFF4"><ce:sup>d</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jean-Jacques</ce:given-name><ce:surname>Toulme</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Laboratoire de Biophysique Moléculaire, INSERM U386, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux cédex, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Sanofi Elf-Biorecherches, Labège Innopole, BP 137, 31676 Labège cédex, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>EMBL, Meyerhofstrasse 1, D-69012 Heidelberg, Germany</ce:textfn></ce:affiliation><ce:affiliation id="AFF4"><ce:label>d</ce:label><ce:textfn>Centre de Biophysique Moléculaire, CNRS, 45071 Orléans, France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Corresponding author. Fax: + 33 57 571015</ce:text></ce:correspondence></ce:author-group><ce:date-received day="23" month="12" year="1994"></ce:date-received><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>We investigated the properties of two antisense oligonucleotides, 11αPso and 14TMP, 11 and 14 nucleotides long, respectively, and conjugated to psoralen derivatives. These oligonucleotides were complementary to the mini-exon sequence of <ce:italic>Leishmania amazonensis</ce:italic>. Upon ultraviolet (UV) irradiation these oligomers were selectively cross-linked to DNA or RNA target sequences, either 14 or 35 nucleotides long. The yield of photo-addition was much lower on the longer targets than on the shorter ones, due to the presence of a hairpin structure. The co-addition of a helper oligonucleotide, whose binding site, on the 35-mer, was adjacent to that of the psoralen-derivatized antisense oligomer, improved the cross-linking efficiency. We then determined the effect of 14TMP on in vitro translation of <ce:italic>Leishmania</ce:italic> mRNA in cell-free extracts. Non-irradiated antisense oligonucleotide/mRNA complexes reduced the protein synthesis in wheat germ extract but not in rabbit reticulocyte lysate. Conversely, UV irradiation induced a 14TMP-dependent reduction of translation in reticulocyte lysate whereas the inhibition was not improved in the wheat germ extract. These results are discussed with respect to the involvement of RNase-H in the oligonucleotide-mediated effect on protein synthesis.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Antisense oligonucleotide</ce:text></ce:keyword><ce:keyword><ce:text>Cell-free translation</ce:text></ce:keyword><ce:keyword><ce:text>Alpha oligonucleotide</ce:text></ce:keyword><ce:keyword><ce:text>Photosensitizer</ce:text></ce:keyword><ce:keyword><ce:text>Hairpin structure</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of</title></titleInfo><name type="personal"><namePart type="given">Emanuelle</namePart><namePart type="family">Pascolo</namePart><affiliation>Laboratoire de Biophysique Moléculaire, INSERM U386, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux cédex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Denis</namePart><namePart type="family">Hudrisier</namePart><affiliation>Sanofi Elf-Biorecherches, Labège Innopole, BP 137, 31676 Labège cédex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Brian</namePart><namePart type="family">Sproat</namePart><affiliation>EMBL, Meyerhofstrasse 1, D-69012 Heidelberg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nguyen T.</namePart><namePart type="family">Thuong</namePart><affiliation>Centre de Biophysique Moléculaire, CNRS, 45071 Orléans, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean-Jacques</namePart><namePart type="family">Toulme</namePart><affiliation>Corresponding author. Fax: + 33 57 571015</affiliation><affiliation>Laboratoire de Biophysique Moléculaire, INSERM U386, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux cédex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: We investigated the properties of two antisense oligonucleotides, 11αPso and 14TMP, 11 and 14 nucleotides long, respectively, and conjugated to psoralen derivatives. These oligonucleotides were complementary to the mini-exon sequence of Leishmania amazonensis. Upon ultraviolet (UV) irradiation these oligomers were selectively cross-linked to DNA or RNA target sequences, either 14 or 35 nucleotides long. The yield of photo-addition was much lower on the longer targets than on the shorter ones, due to the presence of a hairpin structure. The co-addition of a helper oligonucleotide, whose binding site, on the 35-mer, was adjacent to that of the psoralen-derivatized antisense oligomer, improved the cross-linking efficiency. We then determined the effect of 14TMP on in vitro translation of Leishmania mRNA in cell-free extracts. Non-irradiated antisense oligonucleotide/mRNA complexes reduced the protein synthesis in wheat germ extract but not in rabbit reticulocyte lysate. Conversely, UV irradiation induced a 14TMP-dependent reduction of translation in reticulocyte lysate whereas the inhibition was not improved in the wheat germ extract. These results are discussed with respect to the involvement of RNase-H in the oligonucleotide-mediated effect on protein synthesis.</abstract><note type="content">Section title: Regular paper</note><subject><genre>Keywords</genre><topic>Antisense oligonucleotide</topic><topic>Cell-free translation</topic><topic>Alpha oligonucleotide</topic><topic>Photosensitizer</topic><topic>Hairpin structure</topic></subject><relatedItem type="host"><titleInfo><title>BBA - Gene Structure and Expression</title></titleInfo><titleInfo type="abbreviated"><title>BBAEXP</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued></originInfo><identifier type="ISSN">0167-4781</identifier><identifier type="PII">S0167-4781(00)X0203-5</identifier><part><date>1994</date><detail type="volume"><number>1219</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>249</end></extent><extent unit="pages"><start>98</start><end>106</end></extent></part></relatedItem><identifier type="istex">3245568FFAB7443833309DB61736BFA3E1F3CD79</identifier><identifier type="ark">ark:/67375/6H6-RHHJRVB1-G</identifier><identifier type="DOI">10.1016/0167-4781(94)90251-8</identifier><identifier type="PII">0167-4781(94)90251-8</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RHHJRVB1-G/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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