Biochemical and biological variation of Cydia pomonella (codling Moth) granulosis virus
Identifieur interne : 001D98 ( Istex/Corpus ); précédent : 001D97; suivant : 001D99Biochemical and biological variation of Cydia pomonella (codling Moth) granulosis virus
Auteurs : Jeanne P. Harvey ; Loy E. VolkmanSource :
- Virology [ 0042-6822 ] ; 1983.
English descriptors
- Teeft :
- Acetic acid, Agarose, Agarose slab, Artificial diet, Baculovirus, Bamhi, Biological activity, Biological control, Codling, Codling moth, Cpgv, Cpgvs, Digestion, Dna, Ecori, Edta, Enzymatic digests, Equal volume, Fifth instar, Fifth instar larvae, First instar, First instar larvae, Fragment, Fresh diet, Genome, Granulosis, Granulosis virus, Granulosis viruses, High degree, Hybridization, Incubation buffer, Instar, Kodak film, Large differences, Larva, Molecular weight, Molecular weights, Moth, Nitrocellulose, Nitrocellulose filters, Other granulosis viruses, Paper towels, Pathol, Pieris rapae, Polyhedrosis virus, Polypeptide, Probit analysis, Radioimmune, Radioimmune protein blot, Radiolabeled probe, Restriction, Restriction endonuclease analysis, Restriction enzyme, Restriction enzyme profiles, Restriction fragments, Restriction profiles, Restriction site, Serological reactivity, Serological relatedness, Southern blots, Structural polypeptides, Viral, Viral genomes, Virion, Virion polypeptides, Virology, Virus, Volkman, Weight band, Weight proteins, Xhoi.
Abstract
Abstract: Cydia pomonella granulosis viruses (CpGV) from three different sources were compared biochemically and biologically. Restriction enzyme profiles of a CpGV isolated in Mexico and propagated for 9 years in a laboratory in Darmstadt, Germany (CpGV-MD) were compared with those of the same isolate propagated for 9 years at a Berkeley, California, laboratory (CpGV-MB). Only one of six enzymatic digests revealed a difference in the DNAs, which were subsequently calculated to be 99.94% homologous. No differences were found in molecular weights of enveloped virion polypeptides with SDS-polyacrylamide gel electrophoresis. In contrast, the restriction profiles of a CpGV isolated in Russia (CpGV-R) differed from that of CpGV-MB and CpGV-MD in five of six enzymatic digests due to what appeared to be a deleted fragment of DNA of about 1 × 106 in the CpGV-R genome. SDS-PAGE indicated a small difference in mobility of a high-molecular-weight protein in CpGV-R as compared to CpGV-MB and CpGV-MD. Two low-molecular weight proteins present in both CpGV-MD and CpGV-MB were missing completely in CpGV-R. Southern blot hybridization of restriction endonuclease gels using 32P-labeled CpGV-MB DNA showed homology for all three isolates. A radioimmune protein blot assay revealed no differences in serological reactivity among the three CpGVs. LD50 values for first and fifth instar larvae showed CpGV-MB and CpGV-MD to have similar virulence, while CpGV-R had significantly less.
Url:
DOI: 10.1016/0042-6822(83)90287-8
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ISTEX:83A2F70C87F2FEA5EFAF34A7A738D7B90CFE1A8DLe document en format XML
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Harvey</name><affiliation><mods:affiliation>Department of Entomology and Parasitology, University of California, Berkeley, California 94720, USA</mods:affiliation></affiliation></author><author><name sortKey="Volkman, Loy E" sort="Volkman, Loy E" uniqKey="Volkman L" first="Loy E." last="Volkman">Loy E. Volkman</name><affiliation><mods:affiliation>Department of Entomology and Parasitology, University of California, Berkeley, California 94720, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1983">1983</date><biblScope unit="volume">124</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="21">21</biblScope><biblScope unit="page" to="34">34</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acetic acid</term><term>Agarose</term><term>Agarose slab</term><term>Artificial diet</term><term>Baculovirus</term><term>Bamhi</term><term>Biological activity</term><term>Biological control</term><term>Codling</term><term>Codling moth</term><term>Cpgv</term><term>Cpgvs</term><term>Digestion</term><term>Dna</term><term>Ecori</term><term>Edta</term><term>Enzymatic digests</term><term>Equal volume</term><term>Fifth instar</term><term>Fifth instar larvae</term><term>First instar</term><term>First instar larvae</term><term>Fragment</term><term>Fresh diet</term><term>Genome</term><term>Granulosis</term><term>Granulosis virus</term><term>Granulosis viruses</term><term>High degree</term><term>Hybridization</term><term>Incubation buffer</term><term>Instar</term><term>Kodak film</term><term>Large differences</term><term>Larva</term><term>Molecular weight</term><term>Molecular weights</term><term>Moth</term><term>Nitrocellulose</term><term>Nitrocellulose filters</term><term>Other granulosis viruses</term><term>Paper towels</term><term>Pathol</term><term>Pieris rapae</term><term>Polyhedrosis virus</term><term>Polypeptide</term><term>Probit analysis</term><term>Radioimmune</term><term>Radioimmune protein blot</term><term>Radiolabeled probe</term><term>Restriction</term><term>Restriction endonuclease analysis</term><term>Restriction enzyme</term><term>Restriction enzyme profiles</term><term>Restriction fragments</term><term>Restriction profiles</term><term>Restriction site</term><term>Serological reactivity</term><term>Serological relatedness</term><term>Southern blots</term><term>Structural polypeptides</term><term>Viral</term><term>Viral genomes</term><term>Virion</term><term>Virion polypeptides</term><term>Virology</term><term>Virus</term><term>Volkman</term><term>Weight band</term><term>Weight proteins</term><term>Xhoi</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Cydia pomonella granulosis viruses (CpGV) from three different sources were compared biochemically and biologically. Restriction enzyme profiles of a CpGV isolated in Mexico and propagated for 9 years in a laboratory in Darmstadt, Germany (CpGV-MD) were compared with those of the same isolate propagated for 9 years at a Berkeley, California, laboratory (CpGV-MB). Only one of six enzymatic digests revealed a difference in the DNAs, which were subsequently calculated to be 99.94% homologous. No differences were found in molecular weights of enveloped virion polypeptides with SDS-polyacrylamide gel electrophoresis. In contrast, the restriction profiles of a CpGV isolated in Russia (CpGV-R) differed from that of CpGV-MB and CpGV-MD in five of six enzymatic digests due to what appeared to be a deleted fragment of DNA of about 1 × 106 in the CpGV-R genome. SDS-PAGE indicated a small difference in mobility of a high-molecular-weight protein in CpGV-R as compared to CpGV-MB and CpGV-MD. Two low-molecular weight proteins present in both CpGV-MD and CpGV-MB were missing completely in CpGV-R. Southern blot hybridization of restriction endonuclease gels using 32P-labeled CpGV-MB DNA showed homology for all three isolates. A radioimmune protein blot assay revealed no differences in serological reactivity among the three CpGVs. LD50 values for first and fifth instar larvae showed CpGV-MB and CpGV-MD to have similar virulence, while CpGV-R had significantly less.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>polypeptide</json:string><json:string>granulosis</json:string><json:string>larva</json:string><json:string>instar</json:string><json:string>cpgv</json:string><json:string>genome</json:string><json:string>virion</json:string><json:string>molecular weights</json:string><json:string>digestion</json:string><json:string>ecori</json:string><json:string>nitrocellulose</json:string><json:string>codling</json:string><json:string>dna</json:string><json:string>virology</json:string><json:string>hybridization</json:string><json:string>molecular weight</json:string><json:string>volkman</json:string><json:string>codling moth</json:string><json:string>agarose</json:string><json:string>viral</json:string><json:string>edta</json:string><json:string>pathol</json:string><json:string>bamhi</json:string><json:string>radioimmune</json:string><json:string>baculovirus</json:string><json:string>xhoi</json:string><json:string>cpgvs</json:string><json:string>fifth instar</json:string><json:string>nitrocellulose filters</json:string><json:string>radioimmune protein blot</json:string><json:string>granulosis virus</json:string><json:string>first instar larvae</json:string><json:string>structural polypeptides</json:string><json:string>southern blots</json:string><json:string>fifth instar larvae</json:string><json:string>first instar</json:string><json:string>moth</json:string><json:string>fragment</json:string><json:string>weight proteins</json:string><json:string>virion polypeptides</json:string><json:string>high degree</json:string><json:string>restriction site</json:string><json:string>radiolabeled probe</json:string><json:string>restriction endonuclease analysis</json:string><json:string>biological control</json:string><json:string>restriction profiles</json:string><json:string>viral genomes</json:string><json:string>enzymatic digests</json:string><json:string>paper towels</json:string><json:string>restriction enzyme profiles</json:string><json:string>kodak film</json:string><json:string>acetic acid</json:string><json:string>serological relatedness</json:string><json:string>polyhedrosis virus</json:string><json:string>incubation buffer</json:string><json:string>agarose slab</json:string><json:string>artificial diet</json:string><json:string>restriction fragments</json:string><json:string>large differences</json:string><json:string>probit analysis</json:string><json:string>fresh diet</json:string><json:string>serological reactivity</json:string><json:string>restriction enzyme</json:string><json:string>weight band</json:string><json:string>other granulosis viruses</json:string><json:string>granulosis viruses</json:string><json:string>biological activity</json:string><json:string>pieris rapae</json:string><json:string>equal volume</json:string><json:string>virus</json:string><json:string>restriction</json:string></teeft></keywords><author><json:item><name>Jeanne P. Harvey</name><affiliations><json:string>Department of Entomology and Parasitology, University of California, Berkeley, California 94720, USA</json:string></affiliations></json:item><json:item><name>Loy E. Volkman</name><affiliations><json:string>Department of Entomology and Parasitology, University of California, Berkeley, California 94720, USA</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-BJ1R807R-5</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Cydia pomonella granulosis viruses (CpGV) from three different sources were compared biochemically and biologically. Restriction enzyme profiles of a CpGV isolated in Mexico and propagated for 9 years in a laboratory in Darmstadt, Germany (CpGV-MD) were compared with those of the same isolate propagated for 9 years at a Berkeley, California, laboratory (CpGV-MB). Only one of six enzymatic digests revealed a difference in the DNAs, which were subsequently calculated to be 99.94% homologous. No differences were found in molecular weights of enveloped virion polypeptides with SDS-polyacrylamide gel electrophoresis. In contrast, the restriction profiles of a CpGV isolated in Russia (CpGV-R) differed from that of CpGV-MB and CpGV-MD in five of six enzymatic digests due to what appeared to be a deleted fragment of DNA of about 1 × 106 in the CpGV-R genome. SDS-PAGE indicated a small difference in mobility of a high-molecular-weight protein in CpGV-R as compared to CpGV-MB and CpGV-MD. Two low-molecular weight proteins present in both CpGV-MD and CpGV-MB were missing completely in CpGV-R. Southern blot hybridization of restriction endonuclease gels using 32P-labeled CpGV-MB DNA showed homology for all three isolates. A radioimmune protein blot assay revealed no differences in serological reactivity among the three CpGVs. LD50 values for first and fifth instar larvae showed CpGV-MB and CpGV-MD to have similar virulence, while CpGV-R had significantly less.</abstract><qualityIndicators><score>9.652</score><pdfWordCount>5358</pdfWordCount><pdfCharCount>34143</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>14</pdfPageCount><pdfPageSize>504 x 720 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>221</abstractWordCount><abstractCharCount>1482</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Biochemical and biological variation of Cydia pomonella (codling Moth) granulosis virus</title><pmid><json:string>18644273</json:string></pmid><pii><json:string>0042-6822(83)90287-8</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Ph.D. thesis</title><language><json:string>unknown</json:string></language></serie><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>1983</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0356-X</json:string></pii><volume>124</volume><issue>1</issue><pages><first>21</first><last>34</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1974</json:string><json:string>1963</json:string><json:string>1983</json:string></date><geogName></geogName><orgName><json:string>BRL</json:string><json:string>Bethesda Research Laboratories</json:string><json:string>Sigma Chemical Co.</json:string><json:string>Academic Press, Inc.</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>G. S. Smith</json:string><json:string>Zlatanova</json:string><json:string>Huber</json:string><json:string>PrGV</json:string><json:string>L. Falcon</json:string><json:string>L. A. Falcon</json:string><json:string>M. D. Summers</json:string><json:string>PbGV</json:string><json:string>L.A. Falcon</json:string></persName><placeName><json:string>Germany</json:string><json:string>Darmstadt</json:string><json:string>Berkeley</json:string><json:string>Hungary</json:string><json:string>Netherlands</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Bowen et al. (1979)</json:string><json:string>Jurkovicova et al., 1979</json:string><json:string>Marmur, 1961</json:string><json:string>Kisley and Edelman, 1982</json:string><json:string>Tweeten et al., 1980</json:string><json:string>Finney, 1971</json:string><json:string>Falcon, 1971</json:string><json:string>Summers and Smith (1981)</json:string><json:string>Chauthani et al., 1968</json:string><json:string>Ossowski, 1960</json:string><json:string>Sheppard and Stairs (1977)</json:string><json:string>Rigby et al. (1977)</json:string><json:string>Etzel (1973)</json:string><json:string>Szalay-Marzso and Dezsery, 1975</json:string><json:string>Vlak and Groner 1980</json:string><json:string>Smith and Summers (1981)</json:string><json:string>Smith and Summers 1978</json:string><json:string>Weber and Osborn (1969)</json:string><json:string>Crook, 1981</json:string><json:string>Rohrmann et al.</json:string><json:string>Payne, 1981</json:string><json:string>Vlak et al., 1981</json:string><json:string>Vlak and Groner (1980)</json:string><json:string>Summers and Smith, 1978</json:string><json:string>Laemmli, 1970</json:string><json:string>Miller and Dawson, 1978</json:string><json:string>Renart et al. (1979)</json:string><json:string>Reinganum (1975)</json:string><json:string>Vlak and Groner, 1980</json:string><json:string>McCarthy et al.</json:string><json:string>Schuster, 1965</json:string><json:string>Langridge and Balter, 1981</json:string><json:string>Brown et al., 1977</json:string><json:string>Tanada et al., 1960</json:string><json:string>Shapiro and Ignoffo, 1970</json:string><json:string>Marier et al. (1979)</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-BJ1R807R-5</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1983</publicationDate><copyrightDate>1983</copyrightDate><doi><json:string>10.1016/0042-6822(83)90287-8</json:string></doi><id>83A2F70C87F2FEA5EFAF34A7A738D7B90CFE1A8D</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-BJ1R807R-5/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-BJ1R807R-5/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-BJ1R807R-5/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Biochemical and biological variation of Cydia pomonella (codling Moth) granulosis virus</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1983</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Biochemical and biological variation of Cydia pomonella (codling Moth) granulosis virus</title><author xml:id="author-0000"><persName><forename type="first">Jeanne P.</forename><surname>Harvey</surname></persName><note type="biography">To whom reprint requests should be addressed.</note><affiliation>To whom reprint requests should be addressed.</affiliation><affiliation>Department of Entomology and Parasitology, University of California, Berkeley, California 94720, USA</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Loy E.</forename><surname>Volkman</surname></persName><affiliation>Department of Entomology and Parasitology, University of California, Berkeley, California 94720, USA</affiliation></author><idno type="istex">83A2F70C87F2FEA5EFAF34A7A738D7B90CFE1A8D</idno><idno type="ark">ark:/67375/6H6-BJ1R807R-5</idno><idno type="DOI">10.1016/0042-6822(83)90287-8</idno><idno type="PII">0042-6822(83)90287-8</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0356-X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1983"></date><biblScope unit="volume">124</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="21">21</biblScope><biblScope unit="page" to="34">34</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1983</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Cydia pomonella granulosis viruses (CpGV) from three different sources were compared biochemically and biologically. Restriction enzyme profiles of a CpGV isolated in Mexico and propagated for 9 years in a laboratory in Darmstadt, Germany (CpGV-MD) were compared with those of the same isolate propagated for 9 years at a Berkeley, California, laboratory (CpGV-MB). Only one of six enzymatic digests revealed a difference in the DNAs, which were subsequently calculated to be 99.94% homologous. No differences were found in molecular weights of enveloped virion polypeptides with SDS-polyacrylamide gel electrophoresis. In contrast, the restriction profiles of a CpGV isolated in Russia (CpGV-R) differed from that of CpGV-MB and CpGV-MD in five of six enzymatic digests due to what appeared to be a deleted fragment of DNA of about 1 × 106 in the CpGV-R genome. SDS-PAGE indicated a small difference in mobility of a high-molecular-weight protein in CpGV-R as compared to CpGV-MB and CpGV-MD. Two low-molecular weight proteins present in both CpGV-MD and CpGV-MB were missing completely in CpGV-R. Southern blot hybridization of restriction endonuclease gels using 32P-labeled CpGV-MB DNA showed homology for all three isolates. A radioimmune protein blot assay revealed no differences in serological reactivity among the three CpGVs. LD50 values for first and fifth instar larvae showed CpGV-MB and CpGV-MD to have similar virulence, while CpGV-R had significantly less.</p></abstract></profileDesc><revisionDesc><change when="1983">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-BJ1R807R-5/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>83902878</aid><ce:pii>0042-6822(83)90287-8</ce:pii><ce:doi>10.1016/0042-6822(83)90287-8</ce:doi><ce:copyright type="unknown" year="1983"></ce:copyright></item-info><head><ce:title>Biochemical and biological variation of <ce:italic>Cydia pomonella</ce:italic> (codling Moth) granulosis virus</ce:title><ce:author-group><ce:author><ce:given-name>Jeanne P.</ce:given-name><ce:surname>Harvey</ce:surname><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Loy E.</ce:given-name><ce:surname>Volkman</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Entomology and Parasitology, University of California, Berkeley, California 94720, USA</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>To whom reprint requests should be addressed.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="30" month="3" year="1982"></ce:date-received><ce:date-accepted day="1" month="8" year="1982"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para><ce:italic>Cydia pomonella</ce:italic> granulosis viruses (CpGV) from three different sources were compared biochemically and biologically. Restriction enzyme profiles of a CpGV isolated in Mexico and propagated for 9 years in a laboratory in Darmstadt, Germany (CpGV-MD) were compared with those of the same isolate propagated for 9 years at a Berkeley, California, laboratory (CpGV-MB). Only one of six enzymatic digests revealed a difference in the DNAs, which were subsequently calculated to be 99.94% homologous. No differences were found in molecular weights of enveloped virion polypeptides with SDS-polyacrylamide gel electrophoresis. In contrast, the restriction profiles of a CpGV isolated in Russia (CpGV-R) differed from that of CpGV-MB and CpGV-MD in five of six enzymatic digests due to what appeared to be a deleted fragment of DNA of about 1 × 10<ce:sup>6</ce:sup> in the CpGV-R genome. SDS-PAGE indicated a small difference in mobility of a high-molecular-weight protein in CpGV-R as compared to CpGV-MB and CpGV-MD. Two low-molecular weight proteins present in both CpGV-MD and CpGV-MB were missing completely in CpGV-R. Southern blot hybridization of restriction endonuclease gels using <ce:sup loc="pre">32</ce:sup>P-labeled CpGV-MB DNA showed homology for all three isolates. A radioimmune protein blot assay revealed no differences in serological reactivity among the three CpGVs. LD<ce:inf>50</ce:inf> values for first and fifth instar larvae showed CpGV-MB and CpGV-MD to have similar virulence, while CpGV-R had significantly less.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Biochemical and biological variation of Cydia pomonella (codling Moth) granulosis virus</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Biochemical and biological variation of</title></titleInfo><name type="personal"><namePart type="given">Jeanne P.</namePart><namePart type="family">Harvey</namePart><affiliation>Department of Entomology and Parasitology, University of California, Berkeley, California 94720, USA</affiliation><description>To whom reprint requests should be addressed.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Loy E.</namePart><namePart type="family">Volkman</namePart><affiliation>Department of Entomology and Parasitology, University of California, Berkeley, California 94720, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1983</dateIssued><copyrightDate encoding="w3cdtf">1983</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Cydia pomonella granulosis viruses (CpGV) from three different sources were compared biochemically and biologically. Restriction enzyme profiles of a CpGV isolated in Mexico and propagated for 9 years in a laboratory in Darmstadt, Germany (CpGV-MD) were compared with those of the same isolate propagated for 9 years at a Berkeley, California, laboratory (CpGV-MB). Only one of six enzymatic digests revealed a difference in the DNAs, which were subsequently calculated to be 99.94% homologous. No differences were found in molecular weights of enveloped virion polypeptides with SDS-polyacrylamide gel electrophoresis. In contrast, the restriction profiles of a CpGV isolated in Russia (CpGV-R) differed from that of CpGV-MB and CpGV-MD in five of six enzymatic digests due to what appeared to be a deleted fragment of DNA of about 1 × 106 in the CpGV-R genome. SDS-PAGE indicated a small difference in mobility of a high-molecular-weight protein in CpGV-R as compared to CpGV-MB and CpGV-MD. Two low-molecular weight proteins present in both CpGV-MD and CpGV-MB were missing completely in CpGV-R. Southern blot hybridization of restriction endonuclease gels using 32P-labeled CpGV-MB DNA showed homology for all three isolates. A radioimmune protein blot assay revealed no differences in serological reactivity among the three CpGVs. LD50 values for first and fifth instar larvae showed CpGV-MB and CpGV-MD to have similar virulence, while CpGV-R had significantly less.</abstract><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1983</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0356-X</identifier><part><date>1983</date><detail type="volume"><number>124</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>220</end></extent><extent unit="pages"><start>21</start><end>34</end></extent></part></relatedItem><identifier type="istex">83A2F70C87F2FEA5EFAF34A7A738D7B90CFE1A8D</identifier><identifier type="ark">ark:/67375/6H6-BJ1R807R-5</identifier><identifier type="DOI">10.1016/0042-6822(83)90287-8</identifier><identifier type="PII">0042-6822(83)90287-8</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-BJ1R807R-5/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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