Biochemical and biological variation of Cydia pomonella (codling Moth) granulosis virus
Identifieur interne : 001D98 ( Istex/Corpus ); précédent : 001D97; suivant : 001D99Biochemical and biological variation of Cydia pomonella (codling Moth) granulosis virus
Auteurs : Jeanne P. Harvey ; Loy E. VolkmanSource :
- Virology [ 0042-6822 ] ; 1983.
English descriptors
- Teeft :
- Acetic acid, Agarose, Agarose slab, Artificial diet, Baculovirus, Bamhi, Biological activity, Biological control, Codling, Codling moth, Cpgv, Cpgvs, Digestion, Dna, Ecori, Edta, Enzymatic digests, Equal volume, Fifth instar, Fifth instar larvae, First instar, First instar larvae, Fragment, Fresh diet, Genome, Granulosis, Granulosis virus, Granulosis viruses, High degree, Hybridization, Incubation buffer, Instar, Kodak film, Large differences, Larva, Molecular weight, Molecular weights, Moth, Nitrocellulose, Nitrocellulose filters, Other granulosis viruses, Paper towels, Pathol, Pieris rapae, Polyhedrosis virus, Polypeptide, Probit analysis, Radioimmune, Radioimmune protein blot, Radiolabeled probe, Restriction, Restriction endonuclease analysis, Restriction enzyme, Restriction enzyme profiles, Restriction fragments, Restriction profiles, Restriction site, Serological reactivity, Serological relatedness, Southern blots, Structural polypeptides, Viral, Viral genomes, Virion, Virion polypeptides, Virology, Virus, Volkman, Weight band, Weight proteins, Xhoi.
Abstract
Abstract: Cydia pomonella granulosis viruses (CpGV) from three different sources were compared biochemically and biologically. Restriction enzyme profiles of a CpGV isolated in Mexico and propagated for 9 years in a laboratory in Darmstadt, Germany (CpGV-MD) were compared with those of the same isolate propagated for 9 years at a Berkeley, California, laboratory (CpGV-MB). Only one of six enzymatic digests revealed a difference in the DNAs, which were subsequently calculated to be 99.94% homologous. No differences were found in molecular weights of enveloped virion polypeptides with SDS-polyacrylamide gel electrophoresis. In contrast, the restriction profiles of a CpGV isolated in Russia (CpGV-R) differed from that of CpGV-MB and CpGV-MD in five of six enzymatic digests due to what appeared to be a deleted fragment of DNA of about 1 × 106 in the CpGV-R genome. SDS-PAGE indicated a small difference in mobility of a high-molecular-weight protein in CpGV-R as compared to CpGV-MB and CpGV-MD. Two low-molecular weight proteins present in both CpGV-MD and CpGV-MB were missing completely in CpGV-R. Southern blot hybridization of restriction endonuclease gels using 32P-labeled CpGV-MB DNA showed homology for all three isolates. A radioimmune protein blot assay revealed no differences in serological reactivity among the three CpGVs. LD50 values for first and fifth instar larvae showed CpGV-MB and CpGV-MD to have similar virulence, while CpGV-R had significantly less.
Url:
DOI: 10.1016/0042-6822(83)90287-8
Links to Exploration step
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<front><div type="abstract" xml:lang="en">Abstract: Cydia pomonella granulosis viruses (CpGV) from three different sources were compared biochemically and biologically. Restriction enzyme profiles of a CpGV isolated in Mexico and propagated for 9 years in a laboratory in Darmstadt, Germany (CpGV-MD) were compared with those of the same isolate propagated for 9 years at a Berkeley, California, laboratory (CpGV-MB). Only one of six enzymatic digests revealed a difference in the DNAs, which were subsequently calculated to be 99.94% homologous. No differences were found in molecular weights of enveloped virion polypeptides with SDS-polyacrylamide gel electrophoresis. In contrast, the restriction profiles of a CpGV isolated in Russia (CpGV-R) differed from that of CpGV-MB and CpGV-MD in five of six enzymatic digests due to what appeared to be a deleted fragment of DNA of about 1 × 106 in the CpGV-R genome. SDS-PAGE indicated a small difference in mobility of a high-molecular-weight protein in CpGV-R as compared to CpGV-MB and CpGV-MD. Two low-molecular weight proteins present in both CpGV-MD and CpGV-MB were missing completely in CpGV-R. Southern blot hybridization of restriction endonuclease gels using 32P-labeled CpGV-MB DNA showed homology for all three isolates. A radioimmune protein blot assay revealed no differences in serological reactivity among the three CpGVs. LD50 values for first and fifth instar larvae showed CpGV-MB and CpGV-MD to have similar virulence, while CpGV-R had significantly less.</div>
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<abstract>Abstract: Cydia pomonella granulosis viruses (CpGV) from three different sources were compared biochemically and biologically. Restriction enzyme profiles of a CpGV isolated in Mexico and propagated for 9 years in a laboratory in Darmstadt, Germany (CpGV-MD) were compared with those of the same isolate propagated for 9 years at a Berkeley, California, laboratory (CpGV-MB). Only one of six enzymatic digests revealed a difference in the DNAs, which were subsequently calculated to be 99.94% homologous. No differences were found in molecular weights of enveloped virion polypeptides with SDS-polyacrylamide gel electrophoresis. In contrast, the restriction profiles of a CpGV isolated in Russia (CpGV-R) differed from that of CpGV-MB and CpGV-MD in five of six enzymatic digests due to what appeared to be a deleted fragment of DNA of about 1 × 106 in the CpGV-R genome. SDS-PAGE indicated a small difference in mobility of a high-molecular-weight protein in CpGV-R as compared to CpGV-MB and CpGV-MD. Two low-molecular weight proteins present in both CpGV-MD and CpGV-MB were missing completely in CpGV-R. Southern blot hybridization of restriction endonuclease gels using 32P-labeled CpGV-MB DNA showed homology for all three isolates. A radioimmune protein blot assay revealed no differences in serological reactivity among the three CpGVs. LD50 values for first and fifth instar larvae showed CpGV-MB and CpGV-MD to have similar virulence, while CpGV-R had significantly less.</abstract>
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<abstract xml:lang="en"><p>Abstract: Cydia pomonella granulosis viruses (CpGV) from three different sources were compared biochemically and biologically. Restriction enzyme profiles of a CpGV isolated in Mexico and propagated for 9 years in a laboratory in Darmstadt, Germany (CpGV-MD) were compared with those of the same isolate propagated for 9 years at a Berkeley, California, laboratory (CpGV-MB). Only one of six enzymatic digests revealed a difference in the DNAs, which were subsequently calculated to be 99.94% homologous. No differences were found in molecular weights of enveloped virion polypeptides with SDS-polyacrylamide gel electrophoresis. In contrast, the restriction profiles of a CpGV isolated in Russia (CpGV-R) differed from that of CpGV-MB and CpGV-MD in five of six enzymatic digests due to what appeared to be a deleted fragment of DNA of about 1 × 106 in the CpGV-R genome. SDS-PAGE indicated a small difference in mobility of a high-molecular-weight protein in CpGV-R as compared to CpGV-MB and CpGV-MD. Two low-molecular weight proteins present in both CpGV-MD and CpGV-MB were missing completely in CpGV-R. Southern blot hybridization of restriction endonuclease gels using 32P-labeled CpGV-MB DNA showed homology for all three isolates. A radioimmune protein blot assay revealed no differences in serological reactivity among the three CpGVs. LD50 values for first and fifth instar larvae showed CpGV-MB and CpGV-MD to have similar virulence, while CpGV-R had significantly less.</p>
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<head><ce:title>Biochemical and biological variation of <ce:italic>Cydia pomonella</ce:italic>
(codling Moth) granulosis virus</ce:title>
<ce:author-group><ce:author><ce:given-name>Jeanne P.</ce:given-name>
<ce:surname>Harvey</ce:surname>
<ce:cross-ref refid="COR1"><ce:sup>1</ce:sup>
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<ce:author><ce:given-name>Loy E.</ce:given-name>
<ce:surname>Volkman</ce:surname>
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<ce:affiliation><ce:textfn>Department of Entomology and Parasitology, University of California, Berkeley, California 94720, USA</ce:textfn>
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<ce:abstract><ce:section-title>Abstract</ce:section-title>
<ce:abstract-sec><ce:simple-para><ce:italic>Cydia pomonella</ce:italic>
granulosis viruses (CpGV) from three different sources were compared biochemically and biologically. Restriction enzyme profiles of a CpGV isolated in Mexico and propagated for 9 years in a laboratory in Darmstadt, Germany (CpGV-MD) were compared with those of the same isolate propagated for 9 years at a Berkeley, California, laboratory (CpGV-MB). Only one of six enzymatic digests revealed a difference in the DNAs, which were subsequently calculated to be 99.94% homologous. No differences were found in molecular weights of enveloped virion polypeptides with SDS-polyacrylamide gel electrophoresis. In contrast, the restriction profiles of a CpGV isolated in Russia (CpGV-R) differed from that of CpGV-MB and CpGV-MD in five of six enzymatic digests due to what appeared to be a deleted fragment of DNA of about 1 × 10<ce:sup>6</ce:sup>
in the CpGV-R genome. SDS-PAGE indicated a small difference in mobility of a high-molecular-weight protein in CpGV-R as compared to CpGV-MB and CpGV-MD. Two low-molecular weight proteins present in both CpGV-MD and CpGV-MB were missing completely in CpGV-R. Southern blot hybridization of restriction endonuclease gels using <ce:sup loc="pre">32</ce:sup>
P-labeled CpGV-MB DNA showed homology for all three isolates. A radioimmune protein blot assay revealed no differences in serological reactivity among the three CpGVs. LD<ce:inf>50</ce:inf>
values for first and fifth instar larvae showed CpGV-MB and CpGV-MD to have similar virulence, while CpGV-R had significantly less.</ce:simple-para>
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<abstract lang="en">Abstract: Cydia pomonella granulosis viruses (CpGV) from three different sources were compared biochemically and biologically. Restriction enzyme profiles of a CpGV isolated in Mexico and propagated for 9 years in a laboratory in Darmstadt, Germany (CpGV-MD) were compared with those of the same isolate propagated for 9 years at a Berkeley, California, laboratory (CpGV-MB). Only one of six enzymatic digests revealed a difference in the DNAs, which were subsequently calculated to be 99.94% homologous. No differences were found in molecular weights of enveloped virion polypeptides with SDS-polyacrylamide gel electrophoresis. In contrast, the restriction profiles of a CpGV isolated in Russia (CpGV-R) differed from that of CpGV-MB and CpGV-MD in five of six enzymatic digests due to what appeared to be a deleted fragment of DNA of about 1 × 106 in the CpGV-R genome. SDS-PAGE indicated a small difference in mobility of a high-molecular-weight protein in CpGV-R as compared to CpGV-MB and CpGV-MD. Two low-molecular weight proteins present in both CpGV-MD and CpGV-MB were missing completely in CpGV-R. Southern blot hybridization of restriction endonuclease gels using 32P-labeled CpGV-MB DNA showed homology for all three isolates. A radioimmune protein blot assay revealed no differences in serological reactivity among the three CpGVs. LD50 values for first and fifth instar larvae showed CpGV-MB and CpGV-MD to have similar virulence, while CpGV-R had significantly less.</abstract>
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