Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus
Identifieur interne : 001D97 ( Istex/Corpus ); précédent : 001D96; suivant : 001D98Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus
Auteurs : Leonard J. Bello ; J. Charles Whitbeck ; William C. LawrenceSource :
- Virology [ 0042-6822 ] ; 1992.
English descriptors
- Teeft :
- Academic press, Bello, Bhvl, Bovine, Bovine herpesvirus, Bovine herpesvirus type, Clone, Cooper sequence, Cooper strain, Current protocols, Davison, Deletion, Endpoint, Herpes, Herpes simplex virus type, Herpesvirus, Homologue, Hsvl, Hybridization, Hybridized, Kinase, Kinase locus, Locus, Mdbk cells, Mittal, Molecular biology, Mrna, Northern blot analysis, Nuclease, Nucleotide, Other herpesviruses, Primer, Probe, Sali fragment, Sequencing, Simplex, Single base substitutions, Synthetic oligonucleotides, Thymidine, Thymidine kinase, Transcript, Transcript analysis, Transcription, Viral, Whitbeck.
Abstract
Abstract: A detailed sequence and transcript analysis of the thymidine kinase (tk) gene of bovine herpesvirus 1 (BHV1, Cooper strain) was carried out. A tk open reading frame (ORF) of 1077 by was identified and compared with tk ORFs previously published for other strains of BHV1. The Cooper sequence was in good agreement with that of strain 03932 but differed significantly from strains 6660 and LA. Reanalysis of the LA tk sequence, however, failed to confirm this difference. Except for five single base substitutions, our results indicate that the Cooper, 03932, and LA strains share the same tk sequence. The size of the tk mRNA was determined by Northern blot analysis. In contrast to the size of other herpesvirus tk mRNAs (∼ 1.5 kb), the BHV1 tk transcript was 4.3 kb. Northern blot analysis indicated that the tk transcript was 3′ coterminal with the downstream 3.1-kb transcript which encodes a BHV1 homologue of the HSV1 H glycoprotein (gH). The 5′ ends of the tk and gH mRNAs were mapped by S1 analysis to positions 135 and 91 bp upstream of their respective translation start sites. The 5′ end of the tk transcript was found to overlap a 5.2-kb transcript with opposite polarity to the tk mRNA.
Url:
DOI: 10.1016/0042-6822(92)90564-6
Links to Exploration step
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<front><div type="abstract" xml:lang="en">Abstract: A detailed sequence and transcript analysis of the thymidine kinase (tk) gene of bovine herpesvirus 1 (BHV1, Cooper strain) was carried out. A tk open reading frame (ORF) of 1077 by was identified and compared with tk ORFs previously published for other strains of BHV1. The Cooper sequence was in good agreement with that of strain 03932 but differed significantly from strains 6660 and LA. Reanalysis of the LA tk sequence, however, failed to confirm this difference. Except for five single base substitutions, our results indicate that the Cooper, 03932, and LA strains share the same tk sequence. The size of the tk mRNA was determined by Northern blot analysis. In contrast to the size of other herpesvirus tk mRNAs (∼ 1.5 kb), the BHV1 tk transcript was 4.3 kb. Northern blot analysis indicated that the tk transcript was 3′ coterminal with the downstream 3.1-kb transcript which encodes a BHV1 homologue of the HSV1 H glycoprotein (gH). The 5′ ends of the tk and gH mRNAs were mapped by S1 analysis to positions 135 and 91 bp upstream of their respective translation start sites. The 5′ end of the tk transcript was found to overlap a 5.2-kb transcript with opposite polarity to the tk mRNA.</div>
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<head><ce:title>Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus</ce:title>
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<ce:abstract-sec><ce:simple-para>A detailed sequence and transcript analysis of the thymidine kinase (<ce:italic>tk</ce:italic>
) gene of bovine herpesvirus 1 (BHV1, Cooper strain) was carried out. A <ce:italic>tk</ce:italic>
open reading frame (ORF) of 1077 by was identified and compared with <ce:italic>tk</ce:italic>
ORFs previously published for other strains of BHV1. The Cooper sequence was in good agreement with that of strain 03932 but differed significantly from strains 6660 and LA. Reanalysis of the LA <ce:italic>tk</ce:italic>
sequence, however, failed to confirm this difference. Except for five single base substitutions, our results indicate that the Cooper, 03932, and LA strains share the same <ce:italic>tk</ce:italic>
sequence. The size of the <ce:italic>tk</ce:italic>
mRNA was determined by Northern blot analysis. In contrast to the size of other herpesvirus <ce:italic>tk</ce:italic>
mRNAs (∼ 1.5 kb), the BHV1 <ce:italic>tk</ce:italic>
transcript was 4.3 kb. Northern blot analysis indicated that the <ce:italic>tk</ce:italic>
transcript was 3′ coterminal with the downstream 3.1-kb transcript which encodes a BHV1 homologue of the HSV1 H glycoprotein (gH). The 5′ ends of the <ce:italic>tk</ce:italic>
and <ce:italic>gH</ce:italic>
mRNAs were mapped by S1 analysis to positions 135 and 91 bp upstream of their respective translation start sites. The 5′ end of the <ce:italic>tk</ce:italic>
transcript was found to overlap a 5.2-kb transcript with opposite polarity to the tk mRNA.</ce:simple-para>
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<mods version="3.6"><titleInfo><title>Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus</title>
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<titleInfo type="alternative" contentType="CDATA"><title>Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus</title>
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<name type="personal"><namePart type="given">Leonard J</namePart>
<namePart type="family">Bello</namePart>
<affiliation>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</affiliation>
<description>To whom reprint requests should be addressed.</description>
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<name type="personal"><namePart type="given">J.</namePart>
<namePart type="family">Charles Whitbeck</namePart>
<affiliation>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</affiliation>
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<name type="personal"><namePart type="given">William C.</namePart>
<namePart type="family">Lawrence</namePart>
<affiliation>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</affiliation>
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<dateIssued encoding="w3cdtf">1992</dateIssued>
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<abstract lang="en">Abstract: A detailed sequence and transcript analysis of the thymidine kinase (tk) gene of bovine herpesvirus 1 (BHV1, Cooper strain) was carried out. A tk open reading frame (ORF) of 1077 by was identified and compared with tk ORFs previously published for other strains of BHV1. The Cooper sequence was in good agreement with that of strain 03932 but differed significantly from strains 6660 and LA. Reanalysis of the LA tk sequence, however, failed to confirm this difference. Except for five single base substitutions, our results indicate that the Cooper, 03932, and LA strains share the same tk sequence. The size of the tk mRNA was determined by Northern blot analysis. In contrast to the size of other herpesvirus tk mRNAs (∼ 1.5 kb), the BHV1 tk transcript was 4.3 kb. Northern blot analysis indicated that the tk transcript was 3′ coterminal with the downstream 3.1-kb transcript which encodes a BHV1 homologue of the HSV1 H glycoprotein (gH). The 5′ ends of the tk and gH mRNAs were mapped by S1 analysis to positions 135 and 91 bp upstream of their respective translation start sites. The 5′ end of the tk transcript was found to overlap a 5.2-kb transcript with opposite polarity to the tk mRNA.</abstract>
<relatedItem type="host"><titleInfo><title>Virology</title>
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<dateIssued encoding="w3cdtf">1992</dateIssued>
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<identifier type="ISSN">0042-6822</identifier>
<identifier type="PII">S0042-6822(00)X0481-3</identifier>
<part><date>1992</date>
<detail type="volume"><number>189</number>
<caption>vol.</caption>
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<detail type="issue"><number>2</number>
<caption>no.</caption>
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<extent unit="issue-pages"><start>407</start>
<end>851</end>
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<extent unit="pages"><start>407</start>
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