Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus
Identifieur interne : 001D97 ( Istex/Corpus ); précédent : 001D96; suivant : 001D98Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus
Auteurs : Leonard J. Bello ; J. Charles Whitbeck ; William C. LawrenceSource :
- Virology [ 0042-6822 ] ; 1992.
English descriptors
- Teeft :
- Academic press, Bello, Bhvl, Bovine, Bovine herpesvirus, Bovine herpesvirus type, Clone, Cooper sequence, Cooper strain, Current protocols, Davison, Deletion, Endpoint, Herpes, Herpes simplex virus type, Herpesvirus, Homologue, Hsvl, Hybridization, Hybridized, Kinase, Kinase locus, Locus, Mdbk cells, Mittal, Molecular biology, Mrna, Northern blot analysis, Nuclease, Nucleotide, Other herpesviruses, Primer, Probe, Sali fragment, Sequencing, Simplex, Single base substitutions, Synthetic oligonucleotides, Thymidine, Thymidine kinase, Transcript, Transcript analysis, Transcription, Viral, Whitbeck.
Abstract
Abstract: A detailed sequence and transcript analysis of the thymidine kinase (tk) gene of bovine herpesvirus 1 (BHV1, Cooper strain) was carried out. A tk open reading frame (ORF) of 1077 by was identified and compared with tk ORFs previously published for other strains of BHV1. The Cooper sequence was in good agreement with that of strain 03932 but differed significantly from strains 6660 and LA. Reanalysis of the LA tk sequence, however, failed to confirm this difference. Except for five single base substitutions, our results indicate that the Cooper, 03932, and LA strains share the same tk sequence. The size of the tk mRNA was determined by Northern blot analysis. In contrast to the size of other herpesvirus tk mRNAs (∼ 1.5 kb), the BHV1 tk transcript was 4.3 kb. Northern blot analysis indicated that the tk transcript was 3′ coterminal with the downstream 3.1-kb transcript which encodes a BHV1 homologue of the HSV1 H glycoprotein (gH). The 5′ ends of the tk and gH mRNAs were mapped by S1 analysis to positions 135 and 91 bp upstream of their respective translation start sites. The 5′ end of the tk transcript was found to overlap a 5.2-kb transcript with opposite polarity to the tk mRNA.
Url:
DOI: 10.1016/0042-6822(92)90564-6
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ISTEX:1DEE90DE5DC0F0E817466B81AFD85694B5EEFF86Le document en format XML
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Charles Whitbeck</name><affiliation><mods:affiliation>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</mods:affiliation></affiliation></author><author><name sortKey="Lawrence, William C" sort="Lawrence, William C" uniqKey="Lawrence W" first="William C." last="Lawrence">William C. 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Bello</name><affiliation><mods:affiliation>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</mods:affiliation></affiliation></author><author><name sortKey="Charles Whitbeck, J" sort="Charles Whitbeck, J" uniqKey="Charles Whitbeck J" first="J." last="Charles Whitbeck">J. Charles Whitbeck</name><affiliation><mods:affiliation>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</mods:affiliation></affiliation></author><author><name sortKey="Lawrence, William C" sort="Lawrence, William C" uniqKey="Lawrence W" first="William C." last="Lawrence">William C. 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A tk open reading frame (ORF) of 1077 by was identified and compared with tk ORFs previously published for other strains of BHV1. The Cooper sequence was in good agreement with that of strain 03932 but differed significantly from strains 6660 and LA. Reanalysis of the LA tk sequence, however, failed to confirm this difference. Except for five single base substitutions, our results indicate that the Cooper, 03932, and LA strains share the same tk sequence. The size of the tk mRNA was determined by Northern blot analysis. In contrast to the size of other herpesvirus tk mRNAs (∼ 1.5 kb), the BHV1 tk transcript was 4.3 kb. Northern blot analysis indicated that the tk transcript was 3′ coterminal with the downstream 3.1-kb transcript which encodes a BHV1 homologue of the HSV1 H glycoprotein (gH). The 5′ ends of the tk and gH mRNAs were mapped by S1 analysis to positions 135 and 91 bp upstream of their respective translation start sites. The 5′ end of the tk transcript was found to overlap a 5.2-kb transcript with opposite polarity to the tk mRNA.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>bhvl</json:string><json:string>mrna</json:string><json:string>hybridized</json:string><json:string>thymidine</json:string><json:string>kinase</json:string><json:string>nuclease</json:string><json:string>herpesvirus</json:string><json:string>herpes</json:string><json:string>sequencing</json:string><json:string>hsvl</json:string><json:string>deletion</json:string><json:string>whitbeck</json:string><json:string>bello</json:string><json:string>homologue</json:string><json:string>mittal</json:string><json:string>endpoint</json:string><json:string>viral</json:string><json:string>transcript</json:string><json:string>davison</json:string><json:string>cooper strain</json:string><json:string>hybridization</json:string><json:string>clone</json:string><json:string>primer</json:string><json:string>transcript analysis</json:string><json:string>northern blot analysis</json:string><json:string>bovine herpesvirus</json:string><json:string>herpes simplex virus type</json:string><json:string>simplex</json:string><json:string>single base substitutions</json:string><json:string>thymidine kinase</json:string><json:string>mdbk cells</json:string><json:string>kinase locus</json:string><json:string>cooper sequence</json:string><json:string>sali fragment</json:string><json:string>other herpesviruses</json:string><json:string>probe</json:string><json:string>transcription</json:string><json:string>locus</json:string><json:string>nucleotide</json:string><json:string>bovine</json:string><json:string>synthetic oligonucleotides</json:string><json:string>academic press</json:string><json:string>current protocols</json:string><json:string>molecular biology</json:string><json:string>bovine herpesvirus type</json:string></teeft></keywords><author><json:item><name>Leonard J Bello</name><affiliations><json:string>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</json:string></affiliations></json:item><json:item><name>J. Charles Whitbeck</name><affiliations><json:string>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</json:string></affiliations></json:item><json:item><name>William C. Lawrence</name><affiliations><json:string>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-N928B7MF-0</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: A detailed sequence and transcript analysis of the thymidine kinase (tk) gene of bovine herpesvirus 1 (BHV1, Cooper strain) was carried out. A tk open reading frame (ORF) of 1077 by was identified and compared with tk ORFs previously published for other strains of BHV1. The Cooper sequence was in good agreement with that of strain 03932 but differed significantly from strains 6660 and LA. Reanalysis of the LA tk sequence, however, failed to confirm this difference. Except for five single base substitutions, our results indicate that the Cooper, 03932, and LA strains share the same tk sequence. The size of the tk mRNA was determined by Northern blot analysis. In contrast to the size of other herpesvirus tk mRNAs (∼ 1.5 kb), the BHV1 tk transcript was 4.3 kb. Northern blot analysis indicated that the tk transcript was 3′ coterminal with the downstream 3.1-kb transcript which encodes a BHV1 homologue of the HSV1 H glycoprotein (gH). The 5′ ends of the tk and gH mRNAs were mapped by S1 analysis to positions 135 and 91 bp upstream of their respective translation start sites. The 5′ end of the tk transcript was found to overlap a 5.2-kb transcript with opposite polarity to the tk mRNA.</abstract><qualityIndicators><score>9.472</score><pdfWordCount>5030</pdfWordCount><pdfCharCount>30884</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>206</abstractWordCount><abstractCharCount>1208</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus</title><pmid><json:string>1322582</json:string></pmid><pii><json:string>0042-6822(92)90564-6</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Methods in Enzymology</title><language><json:string>unknown</json:string></language><volume>Vol. 152</volume><pages><first>611</first><last>632</last></pages><editor><json:item><name>S.L. 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(1991)</json:string><json:string>Kingston, 1987</json:string><json:string>Kahrs, 1977</json:string><json:string>Green and Struhl, 1987</json:string><json:string>Smith et al., 1990</json:string><json:string>Desai et al., 1988</json:string><json:string>Wagner et al., 1981</json:string><json:string>Kit and Qavi, 1983</json:string><json:string>Ludwig, 1983</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-N928B7MF-0</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - microbiologie</json:string></inist></categories><publicationDate>1992</publicationDate><copyrightDate>1992</copyrightDate><doi><json:string>10.1016/0042-6822(92)90564-6</json:string></doi><id>1DEE90DE5DC0F0E817466B81AFD85694B5EEFF86</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-N928B7MF-0/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-N928B7MF-0/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-N928B7MF-0/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1992</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus</title><author xml:id="author-0000"><persName><forename type="first">Leonard J</forename><surname>Bello</surname></persName><note type="biography">To whom reprint requests should be addressed.</note><affiliation>To whom reprint requests should be addressed.</affiliation><affiliation>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</affiliation></author><author xml:id="author-0001"><persName><forename type="first">J.</forename><surname>Charles Whitbeck</surname></persName><affiliation>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</affiliation></author><author xml:id="author-0002"><persName><forename type="first">William C.</forename><surname>Lawrence</surname></persName><affiliation>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</affiliation></author><idno type="istex">1DEE90DE5DC0F0E817466B81AFD85694B5EEFF86</idno><idno type="ark">ark:/67375/6H6-N928B7MF-0</idno><idno type="DOI">10.1016/0042-6822(92)90564-6</idno><idno type="PII">0042-6822(92)90564-6</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0481-3</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992"></date><biblScope unit="volume">189</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="407">407</biblScope><biblScope unit="page" to="414">414</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1992</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: A detailed sequence and transcript analysis of the thymidine kinase (tk) gene of bovine herpesvirus 1 (BHV1, Cooper strain) was carried out. A tk open reading frame (ORF) of 1077 by was identified and compared with tk ORFs previously published for other strains of BHV1. The Cooper sequence was in good agreement with that of strain 03932 but differed significantly from strains 6660 and LA. Reanalysis of the LA tk sequence, however, failed to confirm this difference. Except for five single base substitutions, our results indicate that the Cooper, 03932, and LA strains share the same tk sequence. The size of the tk mRNA was determined by Northern blot analysis. In contrast to the size of other herpesvirus tk mRNAs (∼ 1.5 kb), the BHV1 tk transcript was 4.3 kb. Northern blot analysis indicated that the tk transcript was 3′ coterminal with the downstream 3.1-kb transcript which encodes a BHV1 homologue of the HSV1 H glycoprotein (gH). The 5′ ends of the tk and gH mRNAs were mapped by S1 analysis to positions 135 and 91 bp upstream of their respective translation start sites. The 5′ end of the tk transcript was found to overlap a 5.2-kb transcript with opposite polarity to the tk mRNA.</p></abstract></profileDesc><revisionDesc><change when="1992">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-N928B7MF-0/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>92905646</aid><ce:pii>0042-6822(92)90564-6</ce:pii><ce:doi>10.1016/0042-6822(92)90564-6</ce:doi><ce:copyright type="unknown" year="1992"></ce:copyright></item-info><head><ce:title>Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus</ce:title><ce:author-group><ce:author><ce:given-name>Leonard J</ce:given-name><ce:surname>Bello</ce:surname><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Charles Whitbeck</ce:surname></ce:author><ce:author><ce:given-name>William C.</ce:given-name><ce:surname>Lawrence</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>To whom reprint requests should be addressed.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="23" month="1" year="1992"></ce:date-received><ce:date-accepted day="10" month="4" year="1992"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A detailed sequence and transcript analysis of the thymidine kinase (<ce:italic>tk</ce:italic>) gene of bovine herpesvirus 1 (BHV1, Cooper strain) was carried out. A <ce:italic>tk</ce:italic> open reading frame (ORF) of 1077 by was identified and compared with <ce:italic>tk</ce:italic> ORFs previously published for other strains of BHV1. The Cooper sequence was in good agreement with that of strain 03932 but differed significantly from strains 6660 and LA. Reanalysis of the LA <ce:italic>tk</ce:italic> sequence, however, failed to confirm this difference. Except for five single base substitutions, our results indicate that the Cooper, 03932, and LA strains share the same <ce:italic>tk</ce:italic> sequence. The size of the <ce:italic>tk</ce:italic> mRNA was determined by Northern blot analysis. In contrast to the size of other herpesvirus <ce:italic>tk</ce:italic> mRNAs (∼ 1.5 kb), the BHV1 <ce:italic>tk</ce:italic> transcript was 4.3 kb. Northern blot analysis indicated that the <ce:italic>tk</ce:italic> transcript was 3′ coterminal with the downstream 3.1-kb transcript which encodes a BHV1 homologue of the HSV1 H glycoprotein (gH). The 5′ ends of the <ce:italic>tk</ce:italic> and <ce:italic>gH</ce:italic> mRNAs were mapped by S1 analysis to positions 135 and 91 bp upstream of their respective translation start sites. The 5′ end of the <ce:italic>tk</ce:italic> transcript was found to overlap a 5.2-kb transcript with opposite polarity to the tk mRNA.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus</title></titleInfo><name type="personal"><namePart type="given">Leonard J</namePart><namePart type="family">Bello</namePart><affiliation>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</affiliation><description>To whom reprint requests should be addressed.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Charles Whitbeck</namePart><affiliation>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">William C.</namePart><namePart type="family">Lawrence</namePart><affiliation>Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6049, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: A detailed sequence and transcript analysis of the thymidine kinase (tk) gene of bovine herpesvirus 1 (BHV1, Cooper strain) was carried out. A tk open reading frame (ORF) of 1077 by was identified and compared with tk ORFs previously published for other strains of BHV1. The Cooper sequence was in good agreement with that of strain 03932 but differed significantly from strains 6660 and LA. Reanalysis of the LA tk sequence, however, failed to confirm this difference. Except for five single base substitutions, our results indicate that the Cooper, 03932, and LA strains share the same tk sequence. The size of the tk mRNA was determined by Northern blot analysis. In contrast to the size of other herpesvirus tk mRNAs (∼ 1.5 kb), the BHV1 tk transcript was 4.3 kb. Northern blot analysis indicated that the tk transcript was 3′ coterminal with the downstream 3.1-kb transcript which encodes a BHV1 homologue of the HSV1 H glycoprotein (gH). The 5′ ends of the tk and gH mRNAs were mapped by S1 analysis to positions 135 and 91 bp upstream of their respective translation start sites. The 5′ end of the tk transcript was found to overlap a 5.2-kb transcript with opposite polarity to the tk mRNA.</abstract><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0481-3</identifier><part><date>1992</date><detail type="volume"><number>189</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>407</start><end>851</end></extent><extent unit="pages"><start>407</start><end>414</end></extent></part></relatedItem><identifier type="istex">1DEE90DE5DC0F0E817466B81AFD85694B5EEFF86</identifier><identifier type="ark">ark:/67375/6H6-N928B7MF-0</identifier><identifier type="DOI">10.1016/0042-6822(92)90564-6</identifier><identifier type="PII">0042-6822(92)90564-6</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-N928B7MF-0/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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